[Changes of liposomes electrophoretic mobility under the effect of ribonuclease and cytochrome c]. / Izmenenie elektroforeticheskoi podvizhnosti liposom pod vliianiem ribonukleasy i tsitokhroma c.

The effect of ribonuclease and cytochrome c on electrophoretic mobility of liposomes composed of phosphatidylcholine and diphosphatidylglycerol has been studied. zeta-Potential of lipid vesicles was found to decrease in the presence of proteins. Parameters of proteins binding to phospholipids were evaluated from the changes of surface charge density of model membranes. The constants of protein association with phospholipids were calculated to be about 4 x 10(4) M-1 for ribonuclease and about 5.4 x 10(4) M-1 for cytochrome c.