[Detection of Trichomonas vaginalis in men]. / Wykrywalnosc zakazen rzesistkiem pochwowym u mezczyzn.

An infection with T. vaginalis in male patients is difficult to diagnose in case of latent or asymptomatic trichomoniasis. This infection is caused by the round forms of T. vaginalis, which are significantly more frequent in male patients, than trophozoites. Trichomonas vaginalis round forms called pseudocyst due to their specific biological properties are difficult to observe with actually used techniques. It was shown, that round forms of T. vaginalis may be detected by Giemsa modified technique and modified survival technique employing acridine orange. Therefore routine diagnosis of trichomoniasis included direct examination of the discharge from urethra, cultures and survival technique employing acridine orange. In the problematic cases, especially in male patients with the latent or asymptomatic trichomoniasis, full range of methods should be used to examine specimen collected from the each part of the urinary tract.

[Virulence of Trichomonas vaginalis strains in relation to clinical forms of Trichomonas vaginitis]. / Zjadliwosc szczepów Trichomonas vaginalis w zaleznosci od postaci klinicznej rzesistkowicy.

Estimation of virulence of T. vaginalis strains injected subcutaneously to mice was done. T. vaginalis strains were obtained from the urogenital tracts of patients with different clinical forms of trichomoniasis. The virulence of T. vaginalis strains measured by infiltration size was found to depend upon a clinical form of trichomoniasis . It was moreover shown that the cutaneous infiltration size is proportional to a dose of Trichomonas administered subcutaneously. Histopathological analysis of cutaneous infiltration itself is not decisive as to the strains virulence, yet in addition to macroscopic examination it may be a criterium in the studies on strain pathogenicity.

[Usefulness of polysaccharide and glycoprotein fractions of Trichomonas vaginalis in the serodiagnosis of Trichomonas infections]. / Przydatnosc frakcji polisacharydowej i glikoproteidowej Trichomonas vaginalis w serodiagnostyce rzesistkowicy.

It has been shown that glycoprotein and polysaccharide fractions obtained from T. vaginalis cells can be employed as antigens in serodiagnosis of trichomoniasis. This refers especially to the polysaccharide fraction because of its easy availability, solubility in aqueous solutions, high diagnostic specificity and sensitivity. It has been stated that the diagnostic value of polysaccharide antigen of T. vaginalis, in occult trichomoniasis in men particularly, is higher than the effect of the secretion examination by means of culture, even after the prostatic gland massage.

[Mixed infections of the urogenital tract with Trichomonas vaginalis and yeast-like fungi in humans with reference to treatment]. / Mischinfektionen des Urogenitaltraktes mit Trichomonas vaginalis und hefeähnlichen Pilzen beim Menschen unter Berücksichtigung der Behandlung.

Comparative studies on the incidence of mixed infections with T. vaginalis and yeast-like fungi in man carried out within the years 1969-1980 and 1981-1984 showed a progressive increase of this kind of disease. Clinical treatment with Clotrimazole was performed in 35 patients. Local therapy with Clotrimazole was effective, if 3-5 days later the patients receive Tinidazole at one dosage. In some patients, the therapy has to be continued with Metronidazole.

[Trichomoniasis of the urogenital tract in patients hospitalized in 1976-1983]. / Trichomoniase des Urogenitaltraktes bei hospitalisierten Kranken in den Jahren 1976-1983.

We report on investigations carried out on T. vaginalis and mixed infection (T. vaginalis and yeast-like fungi). Special attention was paid to metronidazol resistance on T. vaginalis strains isolated from patients who had been repeatedly treated with this medicament. Therefore we point to the necessity of defining the patients' susceptibility to this remedy.

[Diagnostic and epidemiologic significance of multinuclear and round forms of Trichomonas vaginalis]. / Diagnostik und epidemiologische Bedeutung der multinuklearen und Rundformen von Trichomonas vaginalis.

Trichomonas are analyzed in the material taken directly from the urogenital tract of male and female patients cultivated on Roiron medium and using different methods for staining the preparations was carried out. It is observed that some of the preparations examined show round nuclear forms of T. vaginalis impossible the detect by laboratory methods used up to now. The techniques used in this study decrease the risk of false diagnosis of these forms of trichomoniasis by less experienced examiners thus facilitating correct diagnosis and therapy. It should be also said, that they are simple and easy to carry out in the laboratory.

[Effect of different cytostatic agents and antibiotics on the biology of Trichomonas in vitro]. / Einfluss verschiedener Zytostatika und Antibiotika auf die Biologie der Trichomonaden in vitro.

The purpose of these examinations was an attempt to elucidate the influence of some cytostatic preparations (Fluorouracil, Triaziquone (Treminon), Mechlorethaminee Oxide Hydrochloride (Mitomen), Methotroxate, Mitomycins, Cyclophosphamide (Endoxan), Mechlorethamin (Nitrogranulogen) and antibiotics (Penicillin, Streptomycin, Aureomycin, Nystatin) on formation of the multinuclear forms of Trichomonas under the conditions of culture on artificial medium and to investigate the cidal and static effects of chosen cytostatics and antibiotics of different species of Trichomonas in vitro. It was found that Mechlorethamine (Nitrogranulogen) and Mitomycins have distinct influence on formation of the multinuclear forms of Trichomonas in culture. Similar effects were observed in the antibiotic group with aureomycin only if applied pure or combined with other antibiotic drugs. In addition, it was shown that Triaziquone (Treminon) exerts the strongest effects on the examined species of Trichomonas.

[Virulence of candida-like fungi, isolated from the urogenital tract (author's transl)]. / Bewertung der Virulenz aus dem Urogenitaltrakt isolierter hefeähnlicher Pilze.

The virulence of candida-like fungi, isolated from the urogenital tract, was determined (enzymatic techniques, morphology, biologic tests in mice). A good correlation between the results of the various techniques and the clinical signs of the patients was found.