Six- and seven-membered 1-oxa-4-aza-2-silacyclanes as possible correctors of adaptational mechanisms.

The biological activity of eight 1-oxa-4-aza-2-silacyclanes with the OSiCH(2)N fragment including 6-membered 2-sila-5-morpholinones (1-3) and 4-acyl-2-silamorpholines (4-6)and previously unknown 7-membered derivatives of salicylic acid (7, 8) was studied. Compounds 1 and 3-6 show the certain antihypoxic action. Compounds 2 (40 mg/kg), 4 (20 mg/kg), 6 (40 mg/kg), 7 (20 mg/kg) and 8 (40 mg/kg) reduce the physical serviceability of intact animals. Compound 1 (20 mg/kg) influences the physical serviceability in a moderate-positive way on the background of chlorophos-poisoning. Compounds 5-8 displayed protective properties against chlorophos-poisoning at the LD(50) dose and compounds 2, 4, 5, 7 at the LD(100) dose. Influence of compounds 1 and 2 on the emotional-research behavior of mice was studied.

[A coagglutination method in the identification of Bordetella genus microbes]. / Metod koaggliutinatsii v identifikatsii mikrobov roda bordetell.

Trials of the coagglutination test for the identification of Bordetella bacteria and for the detection of B. pertussis in the material from healthy and sick children have demonstrated the possibility of using this test for the detection of B. pertussis in mixed cultures on the third day after preliminary inoculation in the growth medium (casein-coal agar) and as a test for the differentiation between B. pertussis and gram-negative bacteria similar to it by their morphologic and serologic characteristics.

[Glutamine synthetase content and immunochemical analysis of different strains of Bordetella pertussis]. / Soderzhanie glutaminsintetazy i immunokhimicheskii analiz razlichnykh shtammov Bordetella pertussis.

In this investigation 3 groups of strains isolated from pertussis patients have been studied: typical (group 1), atypical in their cultural properties (group 2), unidentified Gram-negative bacilli agglutinated by pertussis and parapertussis antitoxins (group 3). Besides, B. pertussis cultures, obtained by subculturing 2 museum strains and 2 newly isolated strains on synthetic casein-charcoal agar with subinhibiting doses of antibiotics or specific immune sera added, have been studied. As indicated by the results of this study, strains belonging to groups 1 and 2 contain glutamine synthetase, while in strains of group 3 this enzyme is absent. In immunoelectrophoresis strains of group 3 have been found to contain not a single antigen similar to the antigens of strains belonging to groups 1 and 2. Electrophoresis in polyacrylamide gel has revealed to differences in the protein spectrum of the strains of these 3 groups. The investigation has shown that the determination of glutamine synthetase and immunoelectrophoresis can be used for the differentiation of B. pertussis from similar Gram-negative bacilli. B. pertussis strains, changed as the result of experiments with antibiotics and specific immune sera, have also been shown to retain their antigenic composition and protein spectrum and to have no essential difference in the content of glutamine synthetase.

[Importance of immunoelectrophoresis in agar and disc electrophoresis in polyacrylamide gel for characterizing different strains of Bordetella pertussis]. / Znachenie metoda immunoélektroforeza v agare i diskovogo élektroforeza v poliakrilamidnom gele dlia kharakteristiki razlichnykh shtammov Bordetella pertussis.

The antigenic composition of typical and atypical B. pertussis strains obtained in the foci of pertussis infection, as well as experimentally obtained antibiotic-resistant B. pertussis strains, has been studied by the methods of immunoelectrophoresis in agar and electrophoresis in polyacrylamide gel (PAAG). Immunoelectrophoresis in agar has been found capable of differentiating B. pertussis culture from a group of unidentified morphologically similar Gram-negative bacilli by their antigenic composition and thus suitable for use as an additional criterion in the identification of atypical B. pertussis strains. PAAG electrophoresis has permitted finding differences in the set of protein antigens in the control strain and in its clones obtained by multiple subculturing in media with antibiotics added.

[Polyacrylamide gel disc electrophoretic study of laboratory and freshly isolated strains of Bordetella pertussis and Bordetella parapertussis]. / Izuchenie laboratornykh i svezhevydelennykh shtammov Bordetella pertussis i Bordetella parapertussis metodom diskovogo élektroforeza v poliakrilamidnom gele.

Differences in the antigenic complexes of laboratory and newly isolated B. pertussis and B. parapertussis strains have established by means of disc electrophoresis in polyacrylamide gel. B. pertussis strains have been found to contain 2 or 3 antigens more. Laboratory and newly isolated B. pertussis strains have been found to differ in antigenic complexes, the antigens in these complexes having different electrophoretic mobility. The antigens detected in newly isolated strains have greater electrophoretic mobility in comparison with that of the antigens detected in laboratory strains. No such differences have been revealed in laboratory and newly isolated B. parapertussis strains. Disc electrophoresis in polyacrylamide gel allows to detect 2 or 3 more antigens than immunoelectrophoresis in agar gel.

[Isolation of fractions from Bordetella parapertussis extracts and their use for preparing an erythrocyte diagnostic preparation]. / Vydelenie fraktsii iz ékstraktov parakokliushnogo mikroba i ispol'zovanie ikh dlia prigotovleniia éritrotsitarnogo diagnosticheskogo preparata.

Soluble parapertusis antigen, serologically active, having hemosensitive properties and containing only 2 antigenic components was obtained by the method of ethanol fractionation of microbial extracts. This method is simple and convenient for production purposes. The antigen thus obtained was used for the production of a highly specific erythrocytic diagnostic preparation (formalinized, liquid). When tested in reaction with animal and human sera, the new diagnostic preparation proved to be sufficiently active and species specific.

[Immunochemical study of the antigenic structure of microbes of the genus Bordetella. 5. Fractionation of the extracts of the parapertussis microbes and the study of the immunochemical and biochemical properties of the isolated fractions]. / Immunokhimicheskoe issledovanie antigennoi struktury mikrobov roda Bordetella. Soobshchenie V. Fraktsionirovanie ekstraktov parakokliushnykh mikrobov i izuchenie immunokhimicheskikh i biokhimicheskikh svoistv vydelennykh fraktsii.

Fractions responsible for the main part of the serological and immunogenic activity differing by the set of antigens were isolated from the salt extracts of parapertussis microbes by gel-filtration on Sephadex G-100, ion exchange chromatography on DEAE-cellulose, and preparative electrophoresis in agar. Fractions, disclosing a sufficiently high serological activity and possessing the immunological properties, but containing the minimal set (2--3 out of 7) antigens, which were included in the initial extract, were isolated in the agar gel in the use of the preparative electrophoresis method.

[Immunochemical study of the antigenic structure of bacteria of genus Bordetella. IV. Fractionation of B. pertussis extracts and study of the immunochemical and biological properties of the isolated fractions]. / Immunokhimicheskoe issledovanie antigennoi struktury mikrobov roda Bordetella. Soobshchenie IV. Fraktsionirovanie ékstraktov kokliushnykh mikrobov i izuchenie immunokhimicheskikh i biologicheskikh svoistv vydelennykh fraktsii

Fractionation of an extract of pertussis microbes was carried out with the aid of gel-filtration on Sephadex G-100, ion-exchange chromatography on DEAE-cellulose, and preparative electrophoresis. Fractions differing in serological, immunogenic activity and the content of antigenic components were isolated. In using the method of gel-filtration of sefadex G-100 the greatest serological, immunogenic and histamine-sensitizing activity was possessed by the high-molecular fraction containing 8 of 11 antigenic components detected in the initial extract. The antigenic components were distributed into 5 fractions by the ion exchange chromatography on DEAE-cellulose. The greatest serological activity was possessed by fractions exiting from the column at the 0.01--0.04M interval of the phosphate buffer concentration. A method of preparative electrophoresis from the pertussis microbes extract was applied and two fractions were isolated from the anode and the cathode zones, each containing 4 antigenic components only, but possessing serological and immunogenic activity and having no histamine-sensitizing properties.