ABSTRACT
It has been shown that UV-A (lambda=320-400 nm) and UV-B (lambda=280-320 nm) inhibit photosynthesis, nitrogen fixation and nitrification. The purpose of this study was to determine the effects, if any, on denitrification in a microbial community inhabiting the intertidal. The community studied is the microbial mat consisting primarily of Lyngbya that inhabits the Pacific marine intertidal, Baja California, Mexico. Rates of denitrification were determined using the acetylene blockage technique. Pseudomonas fluorescens (ATCC #17400) was used as a control organism, and treated similarly to the mat samples. Samples were incubated either beneath a PAR transparent, UV opaque screen (OP3), or a mylar screen to block UV-B, or a UV transparent screen (UVT) for 2 to 3 hours. Sets of samples were also treated with nitrapyrin to inhibit nitrification, or DCMU to inhibit photosynthesis and treated similarly. Denitrification rates were greater in the UV protected samples than in the UV exposed samples the mat samples as well as for the Ps fluorescens cultures. Killed controls exhibited no activity. In the DCMU and nitrapyrin treated samples denitrification rates were the same as in the untreated samples. These data indicate that denitrification is directly inhibited by UV radiation.
Subject(s)
Atmosphere/chemistry , Environmental Microbiology , Nitrogen/metabolism , Nitrous Oxide/metabolism , Ozone/chemistry , Ultraviolet Rays , Ammonia/metabolism , Ammonia/radiation effects , Cyanobacteria/metabolism , Cyanobacteria/radiation effects , Diuron , Greenhouse Effect , Mexico , Nitrous Oxide/analysis , Nitrous Oxide/radiation effects , Oxidation-Reduction , Ozone/analysis , Pacific Ocean , Photosynthesis/radiation effects , Picolines , Pseudomonas fluorescens/metabolism , Pseudomonas fluorescens/radiation effectsABSTRACT
Crystalline salt is generally considered so hostile to most forms of life that it has been used for centuries as a preservative. Here, we present evidence that prokaryotes inhabiting a natural evaporite crust of halite and gypsum are metabolically active while inside the evaporite for at least 10 months. In situ measurements demonstrated that some of these "endoevaporitic" microorganisms (probably the cyanobacterium Synechococcus Nageli) fixed carbon and nitrogen. Denitrification was not observed. Our results quantified the slow microbial activity that can occur in salt crystals. Implications of this study include the possibility that microorganisms found in ancient evaporite deposits may have been part of an evaporite community.
Subject(s)
Calcium Sulfate , Carbon/metabolism , Cyanobacteria/metabolism , Environmental Microbiology , Geologic Sediments/microbiology , Sodium Chloride , Chlorophyll/metabolism , Chlorophyll A , Cyanobacteria/growth & development , Mexico , Nitrogen/metabolism , Nitrogen Fixation , Pheophytins/metabolism , Photosynthesis , Seawater , Water MicrobiologyABSTRACT
Biological carbon fixation is an important part of global carbon cycling and ecology. Fixation that took place 3,500 million years ago is recorded in the laminated sedimentary rock structures known as stromatolites, which are fossilized remains of microbial mat communities. Stromatolites are the most abundant type of fossil found in the Proterozoic (2,500 to 590 Myr ago), but they then declined, possibly because of predation and competition. Using modern microbial mats as analogues for ancient stromatolites, we show that the rate of carbon fixation is higher at the greater levels of atmospheric CO2 that were probably present in the past. We suggest that carbon fixation in microbial mats was not carbon-limited during the early Precambrian, but became carbon-limited as the supply of inorganic carbon decreased. Carbon limitation led to a lower rate of carbon fixation, especially towards the end of the Precambrian. Thus, another reason for the decline of the stromatolites could have been a decrease in available CO2.