ABSTRACT
A fascinating question in neuroscience is how sensory stimuli evoke calcium dynamics in neurons. Caenorhabditis elegans is one of the most suitable models for optically recording high-throughput calcium spikes at single-cell resolution. However, calcium imaging in C. elegans is challenging due to the difficulties associated with immobilizing the organism. Currently, methods for immobilizing worms include entrapment in a microfluidic channel, anesthesia, or adhesion to a glass slide. We have developed a new method to immobilize worms by trapping them in sodium alginate gel. The sodium alginate solution (5%), polymerized with divalent ions, effectively immobilizes worms in the gel. This technique is especially useful for imaging neuronal calcium dynamics during olfactory stimulation. The highly porous and transparent nature of alginate gel allows the optical recording of cellular calcium oscillations in neurons when briefly exposed to odor stimulation.
ABSTRACT
Learning and memory are fundamental processes for an organism's normal physiological function. Learning can occur at any stage of the organism's physiological development. Imprinted memories formed during the early developmental stage, unlike learning and memory, can last a lifetime. It is not clear whether these two types of memories are interlinked. In this study, we investigated whether imprinted memory influences adult learning and memory in a C. elegans model system. We trained the worms for short-term (STAM) and long-term associated memory (LTAM) towards butanone (BT) after conditioning them for imprinted memory towards isoamyl alcohol (IAA). We observed that these worms had improved learning abilities. However, functional imaging revealed that the worms had a long-term depression in the firing pattern in the AIY interneuron, indicating that there were significant changes in neuronal excitation pattern after imprinting, which could explain the enhanced behavioural alterations in animals after imprinting.
