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1.
Oncogene ; 28(24): 2337-47, 2009 Jun 18.
Article in English | MEDLINE | ID: mdl-19421152

ABSTRACT

The spleen tyrosine kinase Syk has predominantly been studied in hematopoietic cells in which it is involved in immunoreceptor-mediated signaling. Recently, Syk expression was evidenced in numerous nonhematopoietic cells and shown to be involved in tumor formation and progression. The Syk downstream signaling effectors in nonhematopoietic cells remain, however, to be uncovered, and were investigated using MS-based quantitative phosphoproteomics. Two strategies, based on the inhibition of the Syk catalytic activity and on the loss of Syk expression were employed to identify phosphotyrosine-dependent complexes. Quantitative measurements were obtained on 350 proteins purified with phosphotyrosine affinity columns using the SILAC method. Forty-one proteins are dependent on both Syk expression and catalytic activity and were selected as signaling effectors. They are involved in a variety of biological processes such as signal transduction, cell-cell adhesion and cell polarization. We investigated the functional involvement of Syk in cell-cell adhesion and demonstrated the phosphorylation of E-cadherin and alpha-catenin. In addition, Syk is localized at cell-cell contacts, and Syk-mediated phosphorylation of E-cadherin seems to be important for the proper localization of p120-catenin at adherens junctions. Identification of the biochemical pathways regulated by Syk in human cancer cells will help to uncover its role in tumor formation and progression.


Subject(s)
Intracellular Signaling Peptides and Proteins/metabolism , Phosphoproteins/analysis , Protein-Tyrosine Kinases/metabolism , Proteomics/methods , Signal Transduction , Blotting, Western , Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Breast Neoplasms/pathology , Cadherins/metabolism , Catenins , Cell Adhesion , Cell Adhesion Molecules/genetics , Cell Adhesion Molecules/metabolism , Cell Line, Tumor , Electrophoresis, Polyacrylamide Gel , Fluorescent Antibody Technique , Humans , Immunoprecipitation , Intercellular Junctions/metabolism , Intracellular Signaling Peptides and Proteins/genetics , Phosphoproteins/genetics , Phosphoproteins/metabolism , Phosphorylation , Protein Binding , Protein-Tyrosine Kinases/genetics , RNA, Small Interfering/genetics , Syk Kinase , Transfection , Delta Catenin
2.
Immunity ; 15(5): 715-28, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11728334

ABSTRACT

Dynamic interactions between membrane and cytoskeleton components are crucial for T cell antigen recognition and subsequent cellular activation. We report here that the membrane-microfilament linker ezrin plays an important role in these processes. First, ezrin relocalizes to the contact area between T cells and stimulatory antigen-presenting cells (APCs), accumulating in F-actin-rich membrane protrusions at the periphery of the immunological synapse. Second, T cell receptor (TCR)-mediated intracellular signals are sufficient to induce ezrin relocalization, indicating that this protein is an effector of TCR signaling. Third, overexpression of the membrane binding domain of ezrin perturbs T cell receptor clustering in the T cell-APC contact area and inhibits the activation of nuclear factor for activated T cells (NF-AT).


Subject(s)
Phosphoproteins/immunology , T-Lymphocytes/immunology , Cell Communication/immunology , Cytoskeletal Proteins , Humans , Immunity, Cellular , Jurkat Cells , Lymphocyte Activation , Receptors, Antigen, T-Cell/immunology , Signal Transduction/immunology
3.
J Mol Med (Berl) ; 79(5-6): 254-61, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11485017

ABSTRACT

Limb girdle muscular dystrophies (LGMDs) are a group of clinically heterogeneous genetic diseases characterized by progressive weakness and atrophy of scapular and pelvic muscles, with either a dominant or recessive autosomic mode of inheritance. The first symptoms of the disorder appear during the first 20 years of life and progresses gradually, and a walking disability develops 10-20 years later. The gene responsible for LGMD2A has been identified and encodes calpain 3, a protease expressed mainly in skeletal muscle. Apoptotic myonuclei were recently detected in muscular biopsy specimens of LGMD2A patients, and apoptosis was found to be correlated with altered subcellular distribution of inhibitory protein kappaBalpha (IkappaBalpha) and nuclear factor kappaB (NF-kappaB), resulting in sarcoplasmic sequestration of NF-kappaB. Calpain 3 dependent IkappaBalpha degradation was reconstituted in vitro, supporting a possible in vivo sequence of events leading from calpain 3 deficiency to IkappaBkappa accumulation, prevention of nuclear translocation of NF-kappaB, and ultimately apoptosis. Therefore calpain 3, present in healthy muscle as sarcoplasmic and nuclear forms, may control IkappaBalpha turnover and indirectly regulate NF-kappaB dependent expression of survival genes. Recent data reported from a new model of LGMD2A in mice and from other muscular disorders strengthen understanding of the molecular links between calpain 3 and the Ikappaalpha/NF-kappaB pathway. Finally, in light of the lack of apoptosis observed in inflammatory myopathies, a unifying model for the control of cell survival in muscle is proposed and discussed


Subject(s)
DNA-Binding Proteins/metabolism , I-kappa B Proteins , Isoenzymes , Muscle Proteins , Muscle, Skeletal/metabolism , Muscular Dystrophies/physiopathology , NF-kappa B/metabolism , Animals , Apoptosis , Calpain/deficiency , Calpain/metabolism , Cell Survival , Humans , Models, Biological , Muscle, Skeletal/pathology , Muscle, Skeletal/physiopathology , Muscular Dystrophies/enzymology , Muscular Dystrophies/metabolism , Muscular Dystrophies/pathology , Myositis/metabolism , Myositis/pathology , NF-KappaB Inhibitor alpha
4.
Dev Biol ; 233(1): 161-73, 2001 May 01.
Article in English | MEDLINE | ID: mdl-11319865

ABSTRACT

During mouse blastocyst formation, a layer of outer cells differentiates in less than 48 h into a functional epithelium (the trophectoderm). Ezrin, an actin-binding structural component of microvilli in epithelial cells, is also involved in signal transduction and ionic pump control. In the mouse embryo, ezrin becomes restricted to the apical cortex of all blastomeres at compaction and of outer cells at later stages. Here we investigated the function of ezrin in living embryos during epithelial differentiation using mutant forms of ezrin tagged with green fluorescent protein (GFP). GFP-tagged wild-type ezrin (Ez/GFPc) behaved like endogenous ezrin and did not interfere with development. Deletion of the last 53 amino acids (Delta53/GFP) changed the localization of ezrin: after compaction, Delta53/GFP remained associated with the apical and basolateral cortex in all blastomeres, and its expression slightly disturbed the cavitation process. Finally, full-length ezrin with GFP inserted at position 234 (Ez/GFPi) was localized all around the cortex throughout development, although it was concentrated at the apical pole after compaction. In embryos expressing Ez/GFPi, the duration of the 16-cell stage was reduced, while the onset of cavitation was delayed. Moreover, cavitation was abnormal, and the blastocoele was small and retracted almost completely several times as if there were major leakages of blastocoelic fluid. Our results suggest that, in addition to its role in microvilli organization, ezrin is involved in the formation of a functional epithelium through a still unknown mechanism.


Subject(s)
Blastocyst/ultrastructure , Phosphoproteins/isolation & purification , Animals , Cytoskeletal Proteins , Embryology/methods , Green Fluorescent Proteins , Luminescent Proteins , Mice , Microscopy, Video , Morphogenesis , Mutation , Phosphoproteins/genetics , Phosphoproteins/metabolism , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , Sequence Deletion
5.
Infect Immun ; 69(2): 1061-71, 2001 Feb.
Article in English | MEDLINE | ID: mdl-11160003

ABSTRACT

It is essential to investigate the apical surface properties of both M cells and dome enterocytes to understand the mechanisms involved in the binding of pathogens to M cells. In rabbit appendix tissue, monoclonal antibodies (MAbs) highlight differences between M cells (MAb 58) and dome enterocytes (MAb 214). Such antibodies ultimately recognized intestinal mucin-related epitopes. To further characterize these differences, the labeling patterns obtained with these MAbs were compared to those obtained with other antibodies to intestinal mucins on dissected domes from all gut-associated lymphoid tissues. A glycoprotein recognized by MAb 58 was purified on a CsCl isopycnic density gradient and microsequenced, and its mRNA expression was localized by in situ hybridization. It was identified as the rabbit homologue of human Muc2, i.e., the major mucin secreted in intestine tissue. Two other Muc2 carbohydrate epitopes were also expressed on M cells, although Muc2 mRNA was not detected. All results indicated that M cells express, on their apical membrane, glycoconjugates bearing at least three glycosidic epitopes from Muc2. MAb 214 and MAb 6G2, which recognized a partially characterized mucin expressed on dome enterocytes, were negative markers for M cells in rabbit gut-associated lymphoid tissues. We propose that the presence, on the surface of M cells, of carbohydrates also expressed on Muc2, together with the absence of an enterocyte-associated mucin, could favor pathogen attachment and accessibility to the M-cell luminal membrane.


Subject(s)
Glycocalyx/chemistry , Intestinal Mucosa/chemistry , Mucins/analysis , Animals , Antibodies, Monoclonal/immunology , Centrifugation, Density Gradient , Epitopes , Goats , Humans , Intestinal Mucosa/immunology , Mice , Mucin-2 , Mucins/genetics , Mucins/immunology , Peyer's Patches/chemistry , RNA, Messenger/analysis , Rabbits , Rats
6.
J Cell Biol ; 151(5): 1067-80, 2000 Nov 27.
Article in English | MEDLINE | ID: mdl-11086008

ABSTRACT

The cytoskeleton-membrane linker protein ezrin has been shown to associate with phosphatidyl-inositol 4,5-bisphosphate (PIP(2))-containing liposomes via its NH(2)-terminal domain. Using internal deletions and COOH-terminal truncations, determinants of PIP(2) binding were located to amino acids 12-115 and 233-310. Both regions contain a KK(X)(n)K/RK motif conserved in the ezrin/radixin/moesin family. K/N mutations of residues 253 and 254 or 262 and 263 did not affect cosedimentation of ezrin 1-333 with PIP(2)-containing liposomes, but their combination almost completely abolished the capacity for interaction. Similarly, double mutation of Lys 63, 64 to Asn only partially reduced lipid interaction, but combined with the double mutation K253N, K254N, the interaction of PIP(2) with ezrin 1-333 was strongly inhibited. Similar data were obtained with full-length ezrin. When residues 253, 254, 262, and 263 were mutated in full-length ezrin, the in vitro interaction with the cytoplasmic tail of CD44 was not impaired but was no longer PIP(2) dependent. This construct was also expressed in COS1 and A431 cells. Unlike wild-type ezrin, it was not any more localized to dorsal actin-rich structures, but redistributed to the cytoplasm without strongly affecting the actin-rich structures. We have thus identified determinants of the PIP(2) binding site in ezrin whose mutagenesis correlates with an altered cellular localization.


Subject(s)
Phosphatidylinositol 4,5-Diphosphate/metabolism , Phosphoproteins , Actins/metabolism , Amino Acid Sequence , Animals , Binding Sites/physiology , COS Cells , Cytoplasm/metabolism , Cytoskeletal Proteins , Cytoskeleton/chemistry , Cytoskeleton/metabolism , Hyaluronan Receptors/metabolism , Liposomes/chemistry , Liposomes/metabolism , Molecular Sequence Data , Mutagenesis, Site-Directed/physiology , Phosphoproteins/chemistry , Phosphoproteins/genetics , Phosphoproteins/metabolism , Protein Structure, Quaternary , Protein Structure, Tertiary
7.
Nature ; 406(6797): 742-7, 2000 Aug 17.
Article in English | MEDLINE | ID: mdl-10963601

ABSTRACT

Syk is a protein tyrosine kinase that is widely expressed in haematopoietic cells. It is involved in coupling activated immunoreceptors to downstream signalling events that mediate diverse cellular responses including proliferation, differentiation and phagocytosis. Syk expression has been reported in cell lines of epithelial origin, but its function in these cells remains unknown. Here we show that Syk is commonly expressed in normal human breast tissue, benign breast lesions and low-tumorigenic breast cancer cell lines. Syk messenger RNA and protein, however, are low or undetectable in invasive breast carcinoma tissue and cell lines. Transfection of wild-type Syk into a Syk-negative breast cancer cell line markedly inhibited its tumour growth and metastasis formation in athymic mice. Conversely, overexpression of a kinase-deficient Syk in a Syk-positive breast cancer cell line significantly increased its tumour incidence and growth. Suppression of tumour growth by the reintroduction of Syk appeared to be the result of aberrant mitosis and cytokinesis. We propose that Syk is a potent modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas.


Subject(s)
Breast Neoplasms/enzymology , Breast/enzymology , Enzyme Precursors/physiology , Protein-Tyrosine Kinases/physiology , Animals , Apoptosis , Breast/cytology , Breast Neoplasms/pathology , Catalysis , Cell Division/genetics , Cell Division/physiology , Cell Transformation, Neoplastic , Enzyme Precursors/genetics , Female , Genes, Tumor Suppressor , Humans , In Situ Nick-End Labeling , Intracellular Signaling Peptides and Proteins , Mice , Mice, Nude , Neoplasm Transplantation , Protein-Tyrosine Kinases/genetics , RNA, Messenger/metabolism , Syk Kinase , Transfection , Tumor Cells, Cultured
8.
EMBO J ; 19(2): 199-212, 2000 Jan 17.
Article in English | MEDLINE | ID: mdl-10637224

ABSTRACT

The current study focuses on the molecular mechanisms responsible for actin assembly on a defined membrane surface: the phagosome. Mature phagosomes were surrounded by filamentous actin in vivo in two different cell types. Fluorescence microscopy was used to study in vitro actin nucleation/polymerization (assembly) on the surface of phagosomes isolated from J774 mouse macrophages. In order to prevent non-specific actin polymerization during the assay, fluorescent G-actin was mixed with thymosin beta4. The cytoplasmic side of phagosomes induced de novo assembly and barbed end growth of actin filaments. This activity varied cyclically with the maturation state of phagosomes, both in vivo and in vitro. Peripheral membrane proteins are crucial components of this actin assembly machinery, and we demonstrate a role for ezrin and/or moesin in this process. We propose that this actin assembly process facilitates phagosome/endosome aggregation prior to membrane fusion.


Subject(s)
Actins/metabolism , Intracellular Membranes/metabolism , Microfilament Proteins/metabolism , Phagosomes/metabolism , Phosphoproteins/metabolism , Actins/biosynthesis , Actins/chemistry , Animals , Cell Line , Cytochalasin D/pharmacology , Cytoskeletal Proteins , Cytoskeleton/metabolism , Intracellular Membranes/ultrastructure , Kinetics , Macrophages , Membrane Fusion , Mice , Microscopy, Immunoelectron , Molecular Sequence Data , Phagosomes/drug effects , Phagosomes/ultrastructure , Recombinant Proteins/metabolism , Thymosin/metabolism , Transfection
9.
Trends Cell Biol ; 9(7): 289, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10370247
10.
Trends Cell Biol ; 9(5): 187-92, 1999 May.
Article in English | MEDLINE | ID: mdl-10322453

ABSTRACT

Ezrin, radixin and moesin, collectively known as the ERM proteins, are a group of closely related membrane-cytoskeleton linkers that regulate cell adhesion and cortical morphogenesis. ERM proteins can self-associate through intra- and inter-molecular interactions, and these interactions mask several binding sites on the proteins. ERM activation involves unfolding of the molecule, and allows the protein to bind to plasma membrane components either directly, or indirectly through linker proteins. The discovery that the tumour-suppressor NF2, also known as merlin/schwannomin, is related to ERM proteins has added a new impetus to investigations of their roles. This review discusses current understanding of the structure and function of members of the ERM family of proteins.


Subject(s)
Blood Proteins/physiology , Cell Membrane/physiology , Cytoskeletal Proteins , Membrane Proteins/physiology , Microfilament Proteins/physiology , Phosphoproteins/physiology , Cell Adhesion/physiology , Cell Membrane/chemistry , Humans , Tumor Cells, Cultured/chemistry , Tumor Cells, Cultured/cytology , Tumor Cells, Cultured/physiology
12.
Infect Immun ; 67(1): 357-67, 1999 Jan.
Article in English | MEDLINE | ID: mdl-9864237

ABSTRACT

The biochemical composition of the apical membranes of epithelial M cells overlying the gut-associated lymphoid tissues (GALT) is still largely unknown. We have prepared monoclonal antibodies (MAbs) directed against carbonate-washed plasma membranes from epithelial cells detached with EDTA from rabbit appendix, a tissue particularly rich in GALT. As determined by immunofluorescence microscopy, several MAbs specifically recognized either M cells or enterocyte-like cells of the domes from rabbit appendix, sacculus rotundus, and Peyer's patches. M cells were identified by their large ventral pocket containing lymphoid cells and by specific labeling with antivimentin. Among various characterized MAbs, MAb 104 recognized rabbit immunoglobulins and was used as an apical marker for M cells in the rabbit appendix, MAb 58 selectively stained an integral membrane glycoprotein of greater than 205 kDa located at the apex of M cells, and MAb 214 stained a smaller soluble glycoprotein associated with the apical surfaces from neighboring enterocytes. In addition, both MAbs 58 and 214 also labeled luminal mucus and secretory granules in goblet cells. The selective association of mucin-related molecules at the surfaces of either M cells or enterocyte-like cells of the follicle-associated epithelium suggests that specific carbohydrate antigens are differentially expressed by epithelial cells and could account for the differential binding properties of pathogens.


Subject(s)
Appendix/immunology , Epithelial Cells/immunology , Epitopes/analysis , Intestinal Mucosa/immunology , Mucins/immunology , Peyer's Patches/immunology , Animals , Antibodies, Monoclonal/biosynthesis , Antigens, Bacterial/chemistry , Antigens, Bacterial/ultrastructure , Appendix/chemistry , Appendix/ultrastructure , Bacterial Proteins/chemistry , Bacterial Proteins/ultrastructure , Cell Line , Cell Membrane/immunology , Cell Separation/methods , Edetic Acid , Epithelial Cells/chemistry , Epithelial Cells/ultrastructure , Epitopes/biosynthesis , Epitopes/ultrastructure , Female , Gerbillinae , Immunoglobulin A/analysis , Intestinal Mucosa/chemistry , Intestinal Mucosa/ultrastructure , Mice , Mice, Inbred BALB C , Peyer's Patches/chemistry , Peyer's Patches/ultrastructure , Rabbits
13.
Infect Immun ; 65(11): 4813-21, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9353070

ABSTRACT

Suppression of the host defense is one of the hallmarks of Yersinia enterocolitica infection. This enteric pathogen resists phagocytosis and interferes with macrophage functions from an extracellular localization (oxidative-burst generation and tumor necrosis factor alpha production). In this study, we investigated the fate of the Y. enterocolitica-infected macrophage. We found that murine J774A.1 macrophages and macrophages derived from human monocytes were killed by infection with Y. enterocolitica. Analysis of cellular morphology and DNA fragmentation revealed that macrophage cell death occurs through the induction of apoptosis. A total of 92% +/- 5% (mean +/- standard deviation) of murine J774A.1 macrophages and 74% +/- 6% of human monocyte-derived macrophages underwent apoptosis upon Yersinia infection after 4 and 20 h, respectively. The broad-spectrum caspase inhibitor Z-Val-Ala-DL-Asp-fluoromethylketone blocked completion of the Yersinia-induced apoptotic program but not the surface exposure of phosphatidylserine as an early-stage apoptotic event. Analysis of different Yersinia mutants showed that macrophage apoptosis depends on a functional Y. enterocolitica type III protein secretion system. Apoptotic cell death of macrophages was not related to the YopE-mediated cytotoxic effect of Yersinia, since disruption of actin microfilaments by a Y. enterocolitica strain expressing a restricted repertoire of yop genes, including YopE, did not result in macrophage apoptosis. Furthermore, Yersinia-induced cytotoxic alterations in epithelial HeLa cells, which are conferred by YopE, did not lead to apoptosis. Our data demonstrate for the first time that Y. enterocolitica promotes the apoptosis of macrophages, an effect which is clearly distinct from the morphological alterations mediated by Yersinia on epithelial HeLa cells.


Subject(s)
Apoptosis , Macrophages/pathology , Yersinia enterocolitica/pathogenicity , Actins/metabolism , Amino Acid Chloromethyl Ketones/pharmacology , Animals , Bacterial Outer Membrane Proteins/physiology , Cell Line , DNA Fragmentation , Humans , Mice , Virulence , Yersinia Infections/immunology
14.
Curr Biol ; 7(9): 682-8, 1997 Sep 01.
Article in English | MEDLINE | ID: mdl-9285722

ABSTRACT

BACKGROUND: Changes in cell shape and motility are important manifestations of oncogenic transformation, but the mechanisms underlying these changes and key effector molecules in the cytoskeleton remain unknown. The Fos oncogene induces expression of ezrin, the founder member of the ezrin/radixin/moesin (ERM) protein family, but not expression of the related ERM proteins, suggesting that ezrin has a distinct role in cell transformation. ERM proteins have been suggested to link the plasma membrane to the actin-based cytoskeleton and are substrates and anchoring sites for a variety of protein kinases. Here, we examined the role of ezrin in cellular transformation. RESULTS: Fos-mediated transformation of Rat-1 fibroblasts resulted in an increased expression and hyperphosphorylation of ezrin, and a concomitant increased association of ezrin with the cortical cytoskeleton. We tagged ezrin with green fluorescent protein and examined its distribution in normal and Fos-transformed fibroblasts: ezrin was concentrated at the leading edge of extending pseudopodia of Fos-transformed Rat-1 cells, and was mainly cytosolic in normal Rat-1 cells. Functional ablation of ezrin by micro-CALI (chromophore-assisted laser inactivation) blocked plasma-membrane ruffling and motility of Fos-transformed fibroblasts. Ablation of ezrin in normal Rat-1 cells caused a marked collapse of the leading edge of the cell. CONCLUSIONS: Ezrin plays an important role in pseudopodial extension in Fos-transformed Rat-1 fibroblasts, and maintains cell shape in normal Rat-1 cells. The increased expression, hyperphosphorylation and subcellular redistribution of ezrin upon fibroblast transformation coupled with its roles in cell shape and motility suggest a critical role for ezrin in oncogenic transformation.


Subject(s)
Cell Transformation, Neoplastic , Fibroblasts/physiology , Phosphoproteins/physiology , Animals , Cell Membrane/metabolism , Cell Movement , Cell Size , Cytoskeletal Proteins , Electrophoresis, Gel, Two-Dimensional , Fibroblasts/cytology , Lasers , Microscopy, Video , Oncogene Proteins v-fos/metabolism , Phosphoproteins/metabolism , Phosphorylation , Rats
15.
J Biol Chem ; 272(32): 20088-95, 1997 Aug 08.
Article in English | MEDLINE | ID: mdl-9242682

ABSTRACT

Human recombinant ezrin, or truncated forms, were coated in microtiter plate and their capacity to bind actin determined. F-actin bound ezrin with a Kd of 504 +/- 230 nM and a molecular stoichiometry of 10.6 actin per ezrin. Ezrin bound both alpha- and beta/gamma-actin essentially as F-form. F-actin binding was totally prevented or drastically reduced when residues 534-586 or 13-30 were deleted, respectively. An actin binding activity was detected in amino-terminal constructs (ezrin 1-310 and 1-333) provided the glutathione S-transferase moiety of the fusion protein was removed. Series of carboxyl-terminal truncations confirmed the presence of this actin-binding site which bound both F- and G-actin. The F- and G-actin-binding sites were differently sensitive to various chemical effectors and distinct specific ezrin antibodies. The internal actin-binding site was mapped between residues 281 and 333. The association of ezrin amino-terminal fragment to full-length ezrin blocked F-actin binding to ezrin. It is proposed that, in full-length ezrin, the F-actin-binding site required the juxtaposition of the distal-most amino- and carboxyl-terminal residues of the ezrin molecule.


Subject(s)
Actins/metabolism , Phosphoproteins/metabolism , Binding Sites , Cytoskeletal Proteins , Escherichia coli , Humans , Kinetics , Peptide Fragments/metabolism , Peptide Mapping , Protein Binding , Recombinant Proteins/metabolism
16.
Mol Biol Cell ; 8(8): 1543-57, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9285824

ABSTRACT

The ERM proteins--ezrin, radixin, and moesin--are key players in membrane-cytoskeleton interactions. In insect cells infected with recombinant baculoviruses, amino acids 1-115 of ezrin were shown to inhibit an actin- and tubulin-dependent cell-extension activity located in ezrin C-terminal domain (ezrin310-586), whereas full-length ezrin1-586 did not induce any morphological change. To refine the mapping of functional domains of ezrin, 30 additional constructs were overexpressed in Sf9 cells, and the resulting effect of each was qualitatively and semiquantitatively compared. The removal of amino acids 13-30 was sufficient to release a cell-extension phenotype. This effect was abrogated if the 21 distal-most C-terminal amino acids were subsequently deleted (ezrin31-565), confirming the existence of a head-to-tail regulation in the whole molecule. Surprisingly, the deletion in full-length ezrin of the same 21 amino acids provided strong cell-extension competence to ezrin1-565, and this property was recovered in N-terminal constructs as short as ezrin1-310. Within ezrin1-310, amino acid sequences 13-30 and 281-310 were important determinants and acted in cooperation to induce cytoskeleton mobilization. In addition, these same residues are part of a new actin-binding site characterized in vitro in ezrin N-terminal domain.


Subject(s)
Cytoskeleton/physiology , Phosphoproteins/physiology , Animals , Antibody Specificity , Baculoviridae , Blotting, Western , Cell Culture Techniques , Cytoskeletal Proteins , Gene Transfer Techniques , Genetic Vectors , Microfilament Proteins/physiology , Microscopy, Electron, Scanning , Phenotype , Phosphoproteins/genetics , Spodoptera/genetics , Spodoptera/virology
17.
J Cell Sci ; 110 ( Pt 16): 1867-77, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9296387

ABSTRACT

Endocytosis of the Tf/TfR complex is essentially the only pathway active in maturing reticulocytes, while exosomes, formed by invagination of the endosomal membrane, provide a mechanism to eliminate seemingly obsolescent proteins, including the TfR, when their function is completed. In this study, we examined molecular trafficking in the recycling and exosome-directed pathways during endocytosis in maturing reticulocytes. To this end, the flow of two exogenously inserted fluorescent lipid analogs, N-(N-[6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]caproyl]) sphingomyelin (C6-NBD-SM) and N-(lissamine rhodamine B sulfonyl) phosphatidyl ethanolamine (N-Rh-PE) was monitored and compared to that of the transferrin (Tf)/Tf receptor (TfR) complex. Prior to elimination via exosomes, the TfR actively recycles with a half-time of approx. 2 minutes. The recycling kinetics of C6-NBD-SM, as bulk plasma membrane marker, are identical to those of the apoTf/TfR complex, as shown by fluorescence microscopy and biochemical analysis. By contrast, although efficiently internalized along the same pathway, N-Rh-PE does not return to the cell surface. More specifically, sucrose gradient analysis and immunoisolation experiments demonstrated that N-Rh-PE accumulates in exosomes, which are eventually released into the extracellular medium. Fluorometric measurements showed that exogenously inserted N-Rh-PE is present in the reticulocyte plasma membrane as small molecular clusters. Moreover, a close correlation was observed between the fate of crosslinked proteins, including the TfR and acetylcholinesterase (AChE), and the fate of the clustered lipid N-Rh-PE. Thus antibody-induced aggregation of specific proteins like the TfR and AChE, which are normally sorted into exosomes during reticulocyte maturation, enhances their shedding by the exosomal pathway. Taken together, the results support the hypothesis that aggregation of either proteins or lipids act as a general sorting signal for exosomal processing, thereby inhibiting reentry in a recycling pathway and providing an effective means for clearing molecules from the cell surface and their eventual elimination from the cells.


Subject(s)
Coated Vesicles/physiology , Erythrocyte Aggregation/physiology , Reticulocytes/physiology , Transferrin/metabolism , Animals , Endocytosis , Endosomes/physiology , Erythrocyte Membrane/physiology , Erythrocyte Membrane/ultrastructure , Exocytosis , Fluorescent Dyes , Intracellular Membranes/physiology , Models, Biological , Phosphatidylethanolamines , Rats , Rats, Sprague-Dawley , Receptors, Transferrin/metabolism , Reticulocytes/ultrastructure , Rhodamines
18.
Mol Cell Endocrinol ; 129(1): 17-26, 1997 Apr 25.
Article in English | MEDLINE | ID: mdl-9175625

ABSTRACT

The trafficking of the androgen receptor (AR) in transfected cells was studied using a green fluorescent protein (GFP)-AR chimera. The reporter molecule GFP enabled the localization of AR in living cells with a good spatial and temporal resolution. After the construction of GFP-AR and verification of the size of the fusion protein produced, we demonstrated that GFP-AR conserves the functional properties of the AR: GFP-AR had the same androgen-binding affinity as AR, and GFP-AR efficiently transactivated an androgen-responsive gene in response to synthetic androgen at 30 degrees C. The fusion protein was first detected throughout the cytoplasm without hormone, fluorescence becoming nuclear rapidly after androgen incubation. This hormone dependence of AR trafficking was confirmed by the use of the mutant GFP-AR-del4, which lacked the androgen-binding function. The mutant was localized in the cytoplasm in the absence of hormone, but the distribution was not modified by androgen incubation. An ACAS 570 scanning laser cytometer was used to quantify fluorescence in a single living cell, first without and then with hormone. Different hormones and antihormones were tested to determine the dynamics of GFP-AR translocation into the nucleus. All the drugs used were able to induce nuclear translocation, and steady state level was rapidly attained within 1 h. The ratio of receptors in cytoplasmic and nuclear compartments was related to both affinity and concentration of ligand. The data from this follow-up study demonstrated for the first time the intracellular dynamics of the hormone-dependent trafficking of AR in a single living cell.


Subject(s)
Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Receptors, Androgen/genetics , Receptors, Androgen/metabolism , Androgens/metabolism , Animals , Biological Transport, Active , COS Cells , Cell Line , Cell Nucleus/metabolism , Cytoplasm/metabolism , Fluorescence , Green Fluorescent Proteins , Kinetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Transcriptional Activation , Transfection
19.
EMBO J ; 16(1): 35-43, 1997 Jan 02.
Article in English | MEDLINE | ID: mdl-9009265

ABSTRACT

cAMP-dependent protein kinase (A-kinase) anchoring proteins (AKAPs) are responsible for the subcellular sequestration of the type II A-kinase. Previously, we identified a 78 kDa AKAP which was enriched in gastric parietal cells. We have now purified the 78 kDa AKAP to homogeneity from gastric fundic mucosal supernates using type II A-kinase regulatory subunit (RII) affinity chromatography. The purified 78 kDa AKAP was recognized by monoclonal antibodies against ezrin, the canalicular actin-associated protein. Recombinant ezrin produced in either Sf9 cells or bacteria also bound RII. Recombinant radixin and moesin, ezrin-related proteins, also bound RII in blot overlay. Analysis of recombinant truncations of ezrin mapped the RII binding site to a region between amino acids 373 and 439. This region contained a 14-amino-acid amphipathic alpha-helical putative RII binding region. A synthetic peptide containing the amphipathic helical region (ezrin409-438) blocked RII binding to ezrin, but a peptide with a leucine to proline substitution at amino acid 421 failed to inhibit RII binding. In mouse fundic mucosa, RII immunoreactivity redistributed from a predominantly cytosolic location in resting parietal cells, to a canalicular pattern in mucosa from animals stimulated with gastrin. These results demonstrate that ezrin is a major AKAP in gastric parietal cells and may function to tether type II A-kinase to a region near the secretory canaliculus.


Subject(s)
Cyclic AMP-Dependent Protein Kinases/chemistry , Cytoskeletal Proteins/chemistry , Parietal Cells, Gastric/chemistry , Phosphoproteins/chemistry , Amino Acid Sequence , Binding Sites , Cyclic AMP-Dependent Protein Kinase Type II , Cyclic AMP-Dependent Protein Kinases/metabolism , Cytoskeletal Proteins/metabolism , Molecular Sequence Data , Parietal Cells, Gastric/metabolism , Phosphoproteins/metabolism , Protein Binding , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism
20.
Dev Biol ; 177(2): 568-79, 1996 Aug 01.
Article in English | MEDLINE | ID: mdl-8806832

ABSTRACT

During preimplantation development in the mouse, two phenotypically distinct cell populations appear at the 16-cell stage: nonpolarized inner cells that give rise to the inner cell mass and polarized outer cells that give rise mainly to the trophectoderm. The divergence of these two cell lineages is due to asymmetrical cell divisions during the transition from the 8- to the 16 cell stage which can occur following blastomere polarization. During compaction, at the 8-cell stage, cytoplasmic organelles accumulate in the apical domain, a surface pole of microvilli forms, and blastomeres flatten onto one another. During the division from the 8- to the 16-cell stage, the only asymmetrical structure maintained is the pole of microvilli. At the 16-cell stage, only blastomeres inheriting a large part of this apical structure can reestablish a polarized organization. The mechanisms involved in the formation and stabilization of the apical pole of microvilli are still unknown. Ezrin is an actin-associated protein that has been proposed to play a role in the formation of microvillous structures. This led us to study the expression of ezrin during early development of the mouse embryo. We observed that ezrin mRNA and protein are present in the mouse oocyte and throughout preimplantation embryo development, although the amount of protein present decreases continuously during early development, particularly after the 8-cell stage, at the time of compaction. Two isoforms of ezrin phosphorylated on tyrosine residues are present during all of preimplantation development while a third non-tyrosine-phosphorylated isoform appears at the 8-cell stage and its relative amount increases from the 8-cell stage to the blastocyst stage. Before compaction, ezrin is distributed around the cell cortex. However ezrin becomes restricted to the microvilli of the apical pole after compaction. At later stages, ezrin is found in the microvilli of the apical surface of outer cells. Finally, ezrin remains associated with the microvillous pole during the transition from the 8- to 16-cell stage and is found only in the outer cells after division. Thus, ezrin is the first cytocortical protein described that is totally segregated in outer cells at the 16-cell stage after an asymmetrical division.


Subject(s)
Embryonic Development/physiology , Mice/embryology , Phosphoproteins/biosynthesis , RNA, Messenger/analysis , Animals , Base Sequence , Blastocyst/metabolism , Cytoskeletal Proteins , Female , Fertilization in Vitro , Fetal Proteins/chemistry , Membrane Proteins/chemistry , Microvilli/chemistry , Mitosis/physiology , Molecular Sequence Data , Oligonucleotides, Antisense/pharmacology , Phosphoproteins/chemistry , Phosphoproteins/immunology , Pregnancy
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