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1.
Rev Soc Bras Med Trop ; 52: e20180139, 2019 Mar 28.
Article in English | MEDLINE | ID: mdl-30942255

ABSTRACT

INTRODUCTION: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins. METHODS: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity. RESULTS: Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production. CONCLUSIONS: Potato apyrase and SmATPDases have IgE-binding epitopes.


Subject(s)
Antibodies, Helminth/immunology , Apyrase/immunology , Epitopes/immunology , Immunoglobulin E/immunology , Schistosoma mansoni/immunology , Schistosomiasis mansoni/immunology , Solanum tuberosum/enzymology , Animals , Blotting, Western , Cross Reactions , Enzyme-Linked Immunosorbent Assay , Female , Mice, Inbred C57BL
2.
Rev. Soc. Bras. Med. Trop ; 52: e20180139, 2019. graf
Article in English | LILACS | ID: biblio-1041506

ABSTRACT

Abstract INTRODUCTION: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins. METHODS: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity. RESULTS: Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production. CONCLUSIONS: Potato apyrase and SmATPDases have IgE-binding epitopes.


Subject(s)
Animals , Female , Apyrase/immunology , Schistosoma mansoni/immunology , Schistosomiasis mansoni/immunology , Solanum tuberosum/enzymology , Immunoglobulin E/immunology , Antibodies, Helminth/immunology , Epitopes/immunology , Enzyme-Linked Immunosorbent Assay , Blotting, Western , Cross Reactions , Mice, Inbred C57BL
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