Enhancing the effectiveness of Alkaline Phosphatase and bone matrix proteins by tunable metal-organic composite for accelerated mineralization.

The irregular expression of bone matrix proteins occurring during the mineralization of bone regeneration results in various deformities which poses a major concern of orthopedic reconstruction. The limitations of the existing reconstruction practice paved a way for the development of a metal-organic composite [TQ-Sr-Fe] with Metal ions strontium [Sr] and iron [Fe] and a biomolecule Thymoquinone [TQ] in an attempt to enhance the bone mineralization due to their positive significance in osteoblast differentiation, proliferation and maturation. TQ-Sr-Fe was synthesized by in-situ coprecipitation and subjected to various characterization to determine their nature, compatibility and osteogenic efficiency. The crystallographic and electron microscopy analysis reveals sheet like structure of the composite. The negative cytotoxicity of TQ-Sr-Fe in the MG 63 cell line signified their biocompatibility. Cell adhesion and proliferation rate affirmed osteoconductive and osteoinductive nature of the composites and it was further supported by the gene expression of osteoblastic differentiation. The sequential expression of bone matrix proteins such as OCN, SPARC, COL 1, and Alkaline Phosphatase elevate the calcium deposition of MG-63 osteoblast like cells and initiates mineralization compared to control. Thus, the metal-organic composite TQ-Sr-Fe would make a suitable composite for accelerating mineralization process which would leads to faster bone regeneration.

Effects of structural distinction in neodymium nanoparticle for therapeutic application in aberrant angiogenesis.

In the present study we analyzed the effect of structural distinction in neodymium nanostructures for modulating angiogenic process as the strategy for identifying biocompatible Nano therapeutics for biomedical applications. We observed structural dependence of Nd nanoparticles on biocompatibility, the spherical polymorphs showed better biocompatibility when compared with cuboidal and nanorod shaped polymorphs of neodymium. The Nd nanopolymorphs in spherical morphology exhibited least redox modulating effect compared to cuboidal shaped that was higher when compared to Nd nanorods. The efficacy of the Nd Nanopolymorphs to induce biological effect in particular on angiogenic process was observed to be directly related to the polymorphs ability to modulate redox signaling. The redox signaling was observed to be via PKM2-NOX4 signaling pathways. Further the results demonstrated that ROS generated by cuboid and rod shaped nanopolymorphs activated the pro-angiogenic factors namely VE-cadherin, HIF 1α, VEGF and VEGFR-2 to facilitate the angiogenic process. The manuscript highlights the importance of rare earth metal nanoparticles in modulating biological process for therapeutic interventions. The present study opens up a new domain in developing novel biocompatible therapeutics based on rare earth metal nanoparticles for regulating disease pathophysiology.