ABSTRACT
Silica and phosphate based bioactive glass nanoparticles (58SiO2-33CaO-9P2O5) with doping of neem (Azadirachta indica) leaf powder and silver nanoparticles were prepared and characterised. Bioactive glass nanoparticles were produced using sol-gel technique. In vitro bioactivity of the prepared samples was investigated using simulated body fluid. X-ray diffraction (XRD) pattern of prepared glass particles reveals amorphous phase and spherical morphology with a particle size of less than 50 nm. When compared to neem doped glass, better bioactivity was attained in silver doped glass through formation of hydroxyapatite layer on the surface, which was confirmed through XRD, Fourier transform infrared (FTIR), and scanning electron microscopy (SEM) analysis. However, neem leaf powder doped bioactive glass nanoparticles show good antimicrobial activity against Staphylococcus aureus and Escherichia coli and less bioactivity compared with silver doped glass particles. In addition, the biocompatibility of the prepared nanocomposites reveals better results for neem doped and silver doped glasses at lower concentration. Therefore, neem doped bioactive glass may act as a potent antimicrobial agent for preventing microbial infection in tissue engineering applications.
Subject(s)
Anti-Infective Agents/pharmacology , Azadirachta/chemistry , Glass/chemistry , Plant Leaves/chemistry , Body Fluids/drug effects , Cell Line, Tumor , Cell Shape/drug effects , Cell Survival/drug effects , Escherichia coli/drug effects , Humans , Hydrogen-Ion Concentration , Microbial Sensitivity Tests , Nanoparticles/ultrastructure , Powders , Silver/pharmacology , Spectrometry, X-Ray Emission , Spectroscopy, Fourier Transform Infrared , Staphylococcus aureus/drug effects , X-Ray DiffractionABSTRACT
In this present investigation, the colloidal silica and nano ZrO2 embedded silica solution were prepared using sol-gel method followed by the sonication process. The particle size was measured for the prepared silica sol with and without ZrO2 nanoparticles. The prepared nanoparticles were coated on the cotton fabric through pad dry method. The phase and functional group analysis of the cotton fabrics after coating reveals the presence of metal oxides on the surface. The surface morphology of the coated fabrics analysed using SEM shows that the nanoparticles were in spherical morphology with slight agglomerations. The element analysis confirms the presence of silica (SiO2) and ZrO2/SiO2 nanoparticles along with cellulose on the surface. The washing durability of the coated fabrics after 5th and 10th wash indicates that the nanoparticles were strongly adhered on the fabric surface. The burning performance of coated fabrics is in the order of ZrO2/SiO2 (19.5 s) > SiO2 (11.3 s) before and after wash; UV resistance of fabric was in the order of ZrO2/SiO2 > SiO2 > uncoated fabric. Cotton fabrics coated with ZrO2/SiO2 particles show better UV and flammability protection for textile applications.
