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Rapid Detection of KPC, NDM, and OXA-48-Like Carbapenemases by Real-Time PCR from Rectal Swab Surveillance Samples.
Lee, Tracy D; Adie, Kathleen; McNabb, Alan; Purych, Dale; Mannan, Kulvinder; Azana, Robert; Ng, Corrinne; Tang, Patrick; Hoang, Linda M N.
J Clin Microbiol ; 53(8): 2731-3, 2015 Aug.
Article in English | MEDLINE | ID: mdl-26019195

ABSTRACT

We describe a multiplex real-time PCR assay for use on the ABI 7500 Fast TaqMan platform to detect all currently described Klebsiella pneumoniae carbapenemases (KPC), New Delhi metallo-ß-lactamases (NDM), and the OXA-48-like family of carbapenemases from bacterial culture lysates or sample enrichment broth lysates.


Subject(s)
Bacterial Proteins/analysis , Epidemiological Monitoring , Gram-Negative Bacteria/enzymology , Multiplex Polymerase Chain Reaction/methods , Real-Time Polymerase Chain Reaction/methods , Rectum/microbiology , beta-Lactamases/analysis , Bacterial Proteins/genetics , Gram-Negative Bacteria/genetics , Humans , beta-Lactamases/genetics
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