[Experimental model of recurrent acute otitis media: morphological study of the cochlear damage using scanning electron microscopy]. / Modelo experimental de otitis media aguda recurrente: estudio estructural del daño coclear con microscopia electrónica de escáner.

We designed an experimental model of acute otitis media to study its effects in the hair cells of the organ of Corti. Thirty Sprague-Dawley rats were used, and divided into three groups. The rats from the first group were exposed to one episode of acute otitis media, the second group to two episodes and the third group were induced with three episodes of acute otitis media. After that, all cochleas were processed by specific surface specimen method. Finally, each organ of Corti was examined using scanning electron microscopy. Damage to hearing cells was compared between the three groups involved in the study. Significant great loss of sensory cells was found when the episodes of acute otitis media were repeated, in both turns of the cochlea: basal (alpha = 0.002) and distal (alpha = 0.001). The results reported in this study indicate the importance of specialized treatment in children affected with recurrent acute otitis media to prevent cochlear damage.

Modelo experimental de otitis media aguda recurrente: estudio estructural del daño coclear con microscopia electrónica de escáner / Experimental model of recurrent acute otitis media: morphological study of the cochlear damage using scanning electron microscopy

Objetivo: Diseñamos un modelo experimental de otitis media aguda para estudiar la repercusión morfológica sobre las células ciliadas del órgano de Corti. Material y métodos: Treinta ratas Sprague-Dawley fueron divididas en tres grupos a los que se les provocó respectivamente uno, dos y tres episodios de otitis media pneumocócica. Las cócleas fueron procesadas mediante un método específico de preparación de muestras de superficie. Finalmente el órgano de Corti fue estudiado con microscopia electrónica de escáner. Resultados: En cada espira coclear, comparamos la proporción de las células ciliadas perdidas entre los tres grupos del estudio. Los resultados obtenidos demuestran una mayor pérdida de células ciliadas, en espira basal (α = 0,001) y en espira distal (α = 0,002), cuando los episodios de otitis media aguda se repiten. Conclusión: De los resultados obtenidos en el estudio, deducimos la necesidad de un tratamiento especializado de los niños que padecen otitis media aguda recidivante, para prevenir la aparición de un daño coclear irreversible

We designed an experimental model of acute otitis media to study its effects in the hair cells of the organ of Corti. Thirty Sprague-Dawley rats were used, and divided into three groups. The rats from the first group were exposed to one episode of acute otitis media, the second group to two episodes and the third group were induced with three episodes of acute otitis media. After that, all cochleas were processed by specific surface specimen method. Finally, each organ of Corti was examined using scanning electron microscopy. Damage to hearing cells was compared between the three groups involved in the study. Significant great loss of sensory cells was found when the episodes of acute otitis media were repeated, in both turns of the cochlea: basal (α = 0,002) and distal (α = 0,001). The results reported in this study indicate the importance of specialized treatment in children affected with recurrent acute otitis media to prevent cochlear damage