ABSTRACT
The present study searches the effects of international oil prices on the performance of financial sector firms in the Amman Stock Exchange (ASE). Using the daily data which range from July 3, 2006 to April 12, 2018, we found that financial performance of the ASE firms is downturn during this data period and oil prices do not impact on these performances. It is found that downward movement of financial performances in the ASE is totally independent of the movements in international oil prices.
Subject(s)
Financial Management , Petroleum/economics , Commerce , Financial Management/statistics & numerical data , Jordan , Petroleum/statistics & numerical dataABSTRACT
The effects of electrical stimulation on muscle fiber type, meat quality, and composition of Longissimus thoracis muscles from one-humped camels and Dofari Omani cattle of a comparable age range were investigated. A low-voltage electrical stimulation with 90 V, 14 Hz (pulse of 7.5-millisecond duration every 70 milliseconds) 20 min postmortem was applied. Samples from the left muscle were collected from 20 (2 to 3 y) camels and 24 cattle (1 to 3 y). For chemical composition, muscle samples were dried in a freeze dryer, and then ground to determine moisture, protein, fat, and ash. Macro- and micro-minerals were determined using an Inductively Coupled Plasma Emission Spectrometer. Quality characteristics of the meat were evaluated using shear force value, pH, sarcomere, myofibrillar fragmentation index, expressed juice, cooking loss percent, and CIE L*, a*, b* color values. Electrical stimulation resulted in a significantly (P < 0.05) more rapid pH fall in the muscle during the first 24 h after slaughter in both species. Muscles from electrically stimulated carcasses had significantly (P < 0.05) lower ultimate pH, longer sarcomere, and lower shear force values than those from nonstimulated carcasses. Lightness (L*), myofibrillar fragmentation, and expressed juice were significantly (P < 0.05) higher for stimulated than for nonstimulated muscles. Muscles of camels had significantly (P < 0.05) higher expressed juice, cooking loss percent, redness color (a*), and lower fat, Mg, K, and P than those from cattle. Electrical stimulation improved quality characteristics of meat from both species. This indicates that meat quality of local camel and cattle can be improved by electrical stimulation and consequently improves their acceptability to consumers and better marketability.
Subject(s)
Camelus , Cattle , Electric Stimulation/methods , Meat/analysis , Meat/standards , Muscle, Skeletal/physiology , Animals , Consumer Behavior , Hydrogen-Ion Concentration , Microscopy, Electron, Transmission , Muscle Fibers, Skeletal/physiology , Muscle Fibers, Skeletal/ultrastructure , Quality Control , Species SpecificityABSTRACT
A comparison of crude curcuminoid extract and purified curcumin was made to evaluate hepato- and immunoprotective effect of Curcuma longa (turmeric) Zingiberaceae. Carbon tetrachloride (CCl4) induced cellular hepatic damage was evaluated by transmission electron microscopy, hepatic enzymes and thiobarbituric acid reactive species (TBAR) values. A selective cytolytic effect of CCl4 was observed among immature (PNA+) thymocytes and peripheral helper (CD4+) T lymphocytes in spleen and was paralleled by a significant reduction in CD25, CD71 and Con A receptor expression. Treatment with curcuminoid crude extract at two different doses, showed a significant cellular recovery among hepatocytes, which was reflected in a reduction of hepatic enzymes and TBAR values. A significant restoration of lymphocyte viability and CD25, CD71 and Con A receptor expression in both immature (PNA+) thymocytes and splenic helper (CD4+) T lymphocytes was observed. Turmeric crude extract, at both low and high dose, was found to be more efficient as compared to purified curcumin.
Subject(s)
Curcuma/chemistry , Curcumin/pharmacology , Cytoprotection/drug effects , Immunity/drug effects , Oxidative Stress/drug effects , Animals , Antioxidants/pharmacology , Antioxidants/therapeutic use , Carbon Tetrachloride , Carbon Tetrachloride Poisoning , Curcumin/therapeutic use , Hepatocytes/drug effects , Hepatocytes/metabolism , Immunity/physiology , Immunologic Factors/pharmacology , Liver/cytology , Oxidative Stress/immunology , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Rats , Rats, Wistar , T-Lymphocytes/drug effects , T-Lymphocytes/metabolismABSTRACT
Introduction : Le melanome malin primitif naso-sinusien est une affection tres rare et de mauvais pronostic. Elle compte pour environ 90des tumeurs cutaneo-muqueuses et seulement 4des cancers naso-sinusiens. Cas clinique : Les auteurs rapportent un cas de melanome malin des fosses nasales chez une patiente de 53 ans. Le tableau clinique etait domine par une obstruction nasale gauche associee a une epistaxis intermittente. Le diagnostic a ete confirme par l'examen histologique et le traitement a ete base uniquement sur la chimiotherapie. L'evolution etait defavorable avec deces de la patiente a un an de suivi. Discussion : Les melanomes malins des fosses nasales representent 8des melanomes cervico-faciaux. Cette tumeur se voit surtout chez l'adulte a partir de la 6eme decade sans predilection de sexe. Les symptomes les plus communement observes sont l'obstruction nasale et les epistaxis. L'aspect endoscopique est generalement celui d'un bourgeon charnu polypoide tres pigmente brun noir ou achromique rose unilateral. Le traitement reste essentiellement chirurgical consistant une exerese large par voie externe. La radiotherapie et la chimiotherapie sont parfois proposees pour des cas non chirurgicaux ou evolues
Subject(s)
Drug Therapy , Melanoma/diagnosis , Melanoma/radiotherapyABSTRACT
Ring graft is a modified cartilage-perichondrium composite graft (CPCG) with only a peripheral ring shaped cartilage. In this series, tympanic membrane perforations were repaired using (ring graft) during treatment of 18 cases of non-cholesteatomatous chronic suppurative otitis media (CSOM). This study showed that ring graft has the advantages of both CPCG and perichondrial graft but without their disadvantages. Complete closure of the perforations was achieved in all cases without delay in hearing improvement. It is recommended to use the ring graft whenever needed to repair central tympanic membrane perforations even with difficult anterior or total perforations.
Subject(s)
Ear Cartilage/transplantation , Myringoplasty/methods , Tympanic Membrane Perforation/surgery , Humans , Otitis Media, Suppurative/complications , Otitis Media, Suppurative/surgery , Transplantation, Autologous , Treatment Outcome , Tympanic Membrane Perforation/complicationsABSTRACT
Employing a purified lgG fraction of a polyclonal anti-AT1 receptor anti-body, raised against a synthetic octapeptide encompassing residues 14-21 of the first extracellular domain of the AT1 polypeptide, selective AT1 receptor expression was immunohistochemically demonstrable within renal structures in Sprague-Dawley (SD) rats and the desert rodent Meriones crassus. In both animal models, prominent AT1 receptor labelling was evident in renal vascular elements, particularly cortical inter-lobular arteries (IA) as well as vasa recta bundles in the inner stripe of the outer medulla. Less intense labelling was observed among peritubular capillary endothelia within the deep cortex, and at both the outer stripe and the inter-bundle regions of the inner stripe of the outer medulla. The binding of the anti-peptide anti-body was, however, lacking among glomeruli and, except for the intense labelling confined to basement membranes of Bowman's capsule of deep nephrons, was virtually absent in all renal tubular structures of both animal models. Structural assessment of the expressed AT1 receptors by two-dimensional Western blotting revealed that a spectrum of structurally distinct AT1 receptor isoforms is expressed in the renal tissues of both animal models. This spectrum was constituted by isoforms of equal size (70 kDa) but distinct pls in SD rats, and of both different sizes (67-73 kDa) and isoelectric points in M. crassus. In either species, the charge and/or size heterogeneity of AT1 receptor isoforms may be attributed in part to differential post-translational glycosylation mechanisms of the AT1 receptor polypeptide backbone. The potential for the differential glycosylation state of AT1 receptors to alter recognition properties may add another level of complexity to tissue-specific and/or species-specific mechanisms underlying angiotensin II interactions in the kidney.
Subject(s)
Kidney/metabolism , Receptors, Angiotensin/isolation & purification , Animals , Female , Gerbillinae , Immunohistochemistry , Isoelectric Point , Male , Molecular Weight , Protein Isoforms , Rabbits , Rats , Rats, Sprague-Dawley , Species Specificity , Tissue DistributionABSTRACT
The aim of this study was to determine the effects of age on chemical composition and quality characteristics of the Arabian one-humped camel's meat. Samples of longissimus thoracis (between the 10th and the 13th rib of the left side) were randomly collected from 21 Omani intact male camels of three different age groups: group 1 (1-3 years), group 2 (3-5 years) and group 3 (6-8 years). Samples were chilled (1-3°C) for 48h. Moisture, crude protein, fat and ash were determined on freeze dried ground muscle. Mineral contents were determined using an Inductively coupled plasma emission spectrometer (ICP). Meat quality including ultimate muscle pH, Warner-Bratzler shear force, sarcomere length, myofibrillar fragmentation index, expressed juice, cooking loss percent, and colour L(∗), a(∗), b(∗) were measured using standard methods. The moisture, protein, fat and ash ranged from 64.4% to 76.7%; 18.6% to 25.0%, 1.1% to 10.5% and 1.0% to 1.4% on dry matter basis, respectively. The Ca, Mg, Na, K, P, Cad, Cr, Ni, Pb, Co, Mo, Be and V ranged from, 9.2 to 46.6, 24.7 to 57.3, 104.7 to 257.0, 471.4 to 1053.0, 249.9 to 584.0, 0.005 to 0.024, 0.020 to 0.410, 0.016 to 0.187, 0.010 to 0.299, 0.010 to 0.018, 0.050 to 0.470, 0.005 to 0.030 and 0.013 to 0.141mg/100g on dry matter basis, respectively. The percentage of protein decreased and that of fat increased with increasing camel age. The ultimate pH, shear force, sarcomere length, fragmentation index, expressed juice, cooking loss, lightness (L(∗)), redness (a(∗)) and yellowness (b(∗)) ranged from 5.46 to 6.64, 4.25 to 17.82, 0.96 to 2.50, 55.91 to 94.81,19.50 to 33.63, 13.18 to 29.88, 27.86 to 43.21, 10.46 to 22.81, and 4.63 to 10.11, respectively. Muscles of younger camels (group 1) had significantly (P<0.05) lower shear force value, ultimate pH and higher sarcomere length, fragmentation index, expressed juice, cooking loss, and lightness color (L(∗)) by 48%, 3.4%, 43%, 25%, 28%, 14%, and 16% than those collected from older camels (group 3), respectively. Values of middle age camels (group 2) camels were in-between. This study confirmed that camel meat is healthy and nutritious as it contains low fat as well as being a good source of minerals. Age is an important factor in determining meat quality and composition.
ABSTRACT
A monoclonal antibody, specific to all conventional CD45 isoforms, was employed in two-dimensional (2D) sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting to investigate possible age-related differential expression of these isoforms among immature and mature thymocytes as well as CD4+ and CD8+ T cell subpopulations in the periphery of newly-born, young and aged BALB/c mice. In young mice, and to a lesser degree in newly-born mice, intra-thymic maturation seemed to be paralleled by the capacity of thymocytes to synthesize distinct CD45 isoforms constituted by extensively heterogeneous acidic charge entities. Thymocyte maturation in aged mice, on the other hand, was characterized by minimal heterogeneity, as the observed pattern was essentially similar to the immature population in 2D blots. As inferred from comparisons of 2D blots of sialylated and desialylated forms of the CD45 complex, age-related differences in isoforms expressed by the CD4+ and the CD8+ T cell subpopulations in the periphery resided mainly in the degree of sialylation of the constituent isoforms. Given the potential of the differential sialylation state of CD45 in altering the recognition properties of lymphocytes, regulation of CD45 sialylation with age may add another level of complexity to the lymphocyte surface phenotype, which in turn may be implicated in cell-cell interaction mechanisms during lymphocyte maturation and senescence.
Subject(s)
Aging/immunology , Leukocyte Common Antigens/biosynthesis , T-Lymphocytes/immunology , Animals , CD4-Positive T-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Male , Mice , Mice, Inbred BALB C , Protein Conformation , Protein Isoforms/biosynthesisABSTRACT
A Biomphalaria alexandrina-derived lectin (BaSII), of proven specificity to a Schistosoma mansoni-associated fucosyllactose [(Fuc alpha1-2) Gal beta1-4 Glc] determinant, was employed to investigate the putative antigenic cross-reactivity between Schistosoma mansoni and Fasciola hepatica in terms of this structurally-defined oligosaccharide sequence. BaSII affinity column chromatography of extracts of adult worms metabolically radiolabelled with 35S-methionine and analysis by two-dimensional gels established the expression of the fucosyllactose determinant in multiple copies among heterogeneous, acidic glycoproteins synthesized by adult Fasciola hepatica. Direct fluorescence microscopy revealed that determinant-bearing glycoproteins were localized to the external glycocalyx and perikarya of the tegument as well as the epithelial lining of the intestinal caeca and vitelline ducts and glands. Determinant expression was also evident in embryonated cells of eggs and miracidia as well as the intermediate cellular wall of encysted metacercariae, suggesting its conservation during the course of development of the parasite. Based on the structural relatedness of the cross-reactive fucosyllactose determinant to the antigenic mammalian blood group H trisaccharide, our observations may have implications in serodiagnosis and immunoprophylaxis of schistosomiasis/fascioliasis.
Subject(s)
Fasciola hepatica/immunology , Fascioliasis/diagnosis , Lectins/isolation & purification , Schistosoma mansoni/immunology , Schistosomiasis/diagnosis , Snails/parasitology , Animals , Cross Reactions , Fasciola hepatica/isolation & purification , Fascioliasis/prevention & control , Lectins/immunology , Microscopy, Fluorescence , Molecular Weight , Schistosoma mansoni/isolation & purification , Schistosomiasis/prevention & controlABSTRACT
Increasing reports of latex-induced anaphylaxis make preoperative identification of latex-sensitive individuals an important concern. The incidence of latex sensitivity and the efficacy of questionnaires in identifying this in ambulatory surgical populations have not been determined. To clarify these issues, 996 ambulatory surgical patients were studied preoperatively. A questionnaire addressing demographic information, previous surgeries, history of atopy, previous exposure or reactions to latex, congenital abnormalities, and food allergies was administered. These data were then compared with serum anti-latex immunoglobin E (IgE) levels (AlaSTAT test), and risk factors, sensitivity, and specificity were determined. Of this population, 6.7% had IgE antibodies against latex (i.e., latex sensitivity). Male gender, non-Caucasian race, age, asthma, spinal cord abnormalities, food allergies, stated latex allergy, and symptoms when exposed to latex increased the risk of latex sensitivity. The specificity and positive predictive value of history were low. No systemic allergic reactions occurred, a finding that could be attributed to chance alone. The incidence of latex sensitivity in this population suggests that latex allergy is a significant potential problem in ambulatory surgical patients. History, however, does not appear to be a reliable predictor of the presence of anti-latex antibodies.
Subject(s)
Ambulatory Surgical Procedures , Hypersensitivity, Immediate/diagnosis , Immunoglobulin E/blood , Latex/adverse effects , Enzyme-Linked Immunosorbent Assay , Female , Humans , Hypersensitivity, Immediate/etiology , Male , Medical History Taking , Odds Ratio , Predictive Value of Tests , Risk Factors , Sensitivity and Specificity , Surveys and QuestionnairesABSTRACT
STUDY OBJECTIVE: To evaluate the safety and efficacy of single inferior injection peribulbar block administered by anesthesiologists prior to cataract surgery. DESIGN: Retrospective chart review. SETTING: Freestanding surgery center (teaching). PATIENTS: 1,074 consecutive patients who were treated over a two-year period. INTERVENTIONS: Single inferior injection peribulbar block and one inferior peribulbar supplement when indicated. MEASUREMENTS AND MAIN RESULTS: The efficacy of a single inferior injection peribulbar block was 74%; 96% with one inferior peribulbar injection supplement. There were no ocular or systemic complications. CONCLUSION: Single inferior injection peribulbar block is safe and effective when administered by anesthesiologists.
Subject(s)
Anesthesia, Spinal , Cataract Extraction , Nerve Block , Aged , Anesthesia, Spinal/adverse effects , Female , Humans , Male , Nerve Block/adverse effects , Retrospective StudiesABSTRACT
A nested polymerase chain reaction (PCR) protocol was developed for detecting the presence of Schistosoma mansoni sporocysts in intermediate host snails of the genus Biomphalaria. To accomplish this, rDNA genes encoding the 18S rRNA of S. mansoni and Biomphalaria alexandrina from Egypt were sequenced, as were 18S-encoding genes of the 13-16-R1 and Salvador strains of Biomphalaria glabrata. Based on a comparison of host and parasite sequences, a nested set of PCR primers was designed to allow specific amplification of portions of S. mansoni 18S rDNA. These primers allowed detection of as little as 10 fg of S. mansoni DNA diluted in 100 ng of snail DNA and did not allow amplification of snail 18S sequences. Using nested PCR, the presence of a single S. mansoni sporocyst within an adult snail could be detected at 1 day postexposure. In DNA samples extracted from each of 74 snails of the M-line strain of B. glabrata exposed to from 1 to 10 S. mansoni miracidia for intervals ranging from 1 to 44 days, use of the outside primer pair alone detected the parasite's presence in 51% of the snails, whereas the sequential use of outside and nested primer pairs detected parasites in 92% of the snails. This approach has utility in determining if snails in endemic areas bear prepatent or inactive infections and in assessing the degree of compatibility between local snail and schistosome populations. It will also facilitate studies of resistance of snails to infection.
Subject(s)
Biomphalaria/parasitology , DNA, Ribosomal/analysis , Polymerase Chain Reaction , RNA, Ribosomal, 18S/genetics , Schistosoma mansoni/isolation & purification , Animals , Base Sequence , Cloning, Molecular , DNA Primers/chemistry , DNA Primers/genetics , DNA, Helminth/analysis , DNA, Helminth/chemistry , DNA, Ribosomal/chemistry , Female , Male , Molecular Sequence Data , RNA, Helminth/genetics , Schistosoma mansoni/genetics , Sensitivity and Specificity , Sequence AlignmentABSTRACT
Utilizing a Biomphalaria alexandrina-derived lectin (BaSII) of proven specificity to a Schistosoma mansoni-associated fucosyllactose [(Fuc alpha 1-2)Gal beta 1-4 Glc] determinant, a 37-kDa determinant-bearing glycoprotein (Sm 37) was identified selectively on adult male schistosomes. Sm 37 was purified to homogeneity from extracts of adult male worms metabolically radiolabeled with [35S] methionine by BaSII affinity chromatography followed by separation on an HPLC column. Treatments with endoglycosidases, alkaline borohydride, as well as serial lectin affinity chromatography and analysis on 2-dimensional gels indicated that Sm 37 is synthesized as a 33-kDa polypeptide backbone that expresses the fucosyllactose determinant on the outer chain of a single N-linked complex-type glycan unit of either the biantennary or, to a lesser extent, the tri- or tetra-antennary types. The distinct structures of the complex oligosaccharides accounted for the expression of 2 isomorphs of Sm 37. the glycoprotein lacks other conventional high mannose-type or O-linked oligosaccharides and, as deduced from the N-terminal amino acid sequence, the Sm 37 polypeptide may be distinct from other schistosome polypeptides of known sequence. Based on the structural relatedness of the Sm 37-associated fucosyllactose determinant to the antigenic blood group H trisaccharide, these observations may have implications for mechanisms of these host-parasite interactions.
Subject(s)
Glycoproteins/isolation & purification , Helminth Proteins/isolation & purification , Schistosoma mansoni/chemistry , Amino Acid Sequence , Animals , Biomphalaria/chemistry , Chromatography, Affinity , Chromatography, High Pressure Liquid , Electrophoresis, Gel, Two-Dimensional , Electrophoresis, Polyacrylamide Gel , Female , Fluorescein-5-isothiocyanate , Glycoproteins/chemistry , Helminth Proteins/chemistry , Lectins , Male , Microscopy, Fluorescence , Molecular Sequence Data , Sex FactorsABSTRACT
Two alloantisera and a monoclonal antibody (mAb 53-6.7) of proven specificities to the murine Lyt-2/3 macromolecule labeled, in indirect immunofluorescent assays, a distinct lymphocyte population in the toad, Bufo regularis. Lyt-2/3 antigenic activities expressed by B. regularis lymphocytes have been solubilized and purified by mAb 53-6.7 affinity chromatography and found to be associated with a single 67 kDa macromolecule in SDS-PAGE. Upon reduction, this macromolecule resolved into 38 kDa, 34 kDa and 28 kDa subunits corresponding to the alpha, alpha' and beta subunits of the murine Lyt-2/3 complex. Comparisons based on the S delta Q index of differences in amino acid compositions of HPLC-purified alpha- and alpha'-subunits of the amphibian Lyt-2/3 molecule indicated a significant structural relatedness to their murine counterpart as well as to the human CD8 polypeptide. Our observations point to an early phylogenetic emergence of Lyt-2/3 as an important component of the T cell cytolytic apparatus during vertebrate evolution.
Subject(s)
Antigens, Ly/blood , Bufonidae/immunology , Lymphocytes/immunology , Amino Acids/analysis , Animals , Antibodies, Monoclonal , Female , Male , Mice , Molecular Weight , Sequence Homology, Amino AcidABSTRACT
A novel family of isolectins that selectively recognize a schistosome-associated fucosyllactose determinant was identified in the hemolymph of Biomphalaria alexandrina, a snail vector of Schistosoma mansoni. Three lectins of this family were purified by serial affinity chromatography on a column of L-fucose and elution with a gradient of 0.1-1 M L-fucose (designated BaSII and BaSIII), followed by a column of D-glucose and elution with 0.3 M D-glucose (designated BaSI). Assessment of the structural characteristics by one- and two-dimensional gels indicated that, inspite of similarities in native molecular weights, the three lectins were tetramers of noncovalently-associated subunits that were of different sizes and pIs in BaSI, and of equal size but distinct pIs in BaSII and BaSIII. Comparisons of two-dimensional gels of the glycosylated and deglycosylated forms were consistent with the presence of an invariant alpha subunit (13.2 kDa, pI 7.2) constituting the three deglycosylated lectins, which associates with other subunits unique to each lectin, namely a beta subunit (10.1 kDa, pI 5.8) in BaSI, an alpha 1 subunit (13.2 kDa, pI 6.8) in BaSII and BaSIII, and an alpha 2 subunit (13.2 kDa, pI 7.0) in BaSIII. Each of these subunits is subjected to differential post-translational N-linked glycosylations, which accounts for the additional heterogeneity expressed by the glycosylated lectins. Based on miracidial glycoprotein binding and inhibition assays, the three lectins exhibited optimum binding at similar pH and temperature, but were distinct in their binding affinities towards the fucose moiety constituting the fucosyllactose target. These observations indicate that an oligomorphic family of recognition molecules may have evolved to regulate the snails' response to schistosomes.
Subject(s)
Biomphalaria/chemistry , Lectins/isolation & purification , Schistosoma mansoni/chemistry , Animals , Chromatography, Affinity , Electrophoresis, Gel, Two-Dimensional , Hemagglutination Inhibition Tests , Hemolymph/chemistry , Hydrogen-Ion Concentration , Lectins/chemistry , Polysaccharides/metabolism , Protein Binding , TemperatureABSTRACT
Using PNA and anti-Thy-1 fluorescent binding assays, T lymphocytes of the lizard, Chalcides ocellatus were phenotypically distinguishable into four subpopulations (PNA+ Thy-1-, PNA+ Thy-1+, PNA- Thy-1+ and PNA-Thy-1-), which seemed to be affected independently by endogenous steroid levels. Indeed, the size of PNA+ thymocytes is maximal and coincides with the low level of circulating cortisol during spring through summer and decreases gradually with the elevation of the cortisol level. On the other hand, as the endogenous testosterone (TS) level begins its physiological rise, lympholysis of Thy-1+ thymic cells begins in spring with gradual increase in size and with the decrease in TS levels. Among splenocytes and bone marrow lymphocytes, seasonal-dependent alterations in the size of both lymphocyte subpopulations seemed to correlate in part with the status of the thymus. Direct support of this observation was derived from subsequent in vitro studies with exogenous hydrocortisone (HC) and testosterone propionate (TP) treatments in spring and autumn. In all incidents, the data were indicative of the selective susceptibility of the PNA+ Thy-1- subpopulation to HC in the thymus and not in the periphery, and the susceptibility of the PNA- Thy-1+ subpopulation to TP in all three lymphoid organs tested. In vivo studies with a purified fraction of thymosin alpha 1 (T alpha 1) suggested that the PNA+ Thy-1- subpopulation in the different organs was the selective target for the action of T alpha 1. Finally, the dual treatment with T alpha 1 in vivo followed by TP or HC in vitro confirmed that TP-sensitivity was confined to the PNA- Thy-1+ and HC to PNA(+)-Thy-1- subpopulations in any of the three lymphoid organs.
Subject(s)
Hydrocortisone/pharmacology , Seasons , T-Lymphocytes/drug effects , Testosterone/pharmacology , Animals , Antibodies, Monoclonal/chemistry , Arachis/immunology , Binding Sites, Antibody , Cell Differentiation/drug effects , Cell Differentiation/immunology , Cell Survival/drug effects , Cell Survival/immunology , Isoantibodies/chemistry , Lectins/chemistry , Lizards , Male , Peanut Agglutinin , Plant Lectins , Spleen/drug effects , T-Lymphocytes/immunology , Testosterone/blood , Thy-1 Antigens/immunologyABSTRACT
The expression of PNA-binding glycoproteins on lizard lymphocytes was investigated by studying the reactivity of FITC-PNA towards lizard lymphocytes obtained from the different lymphoid organs. Direct immunofluorescence assays have demonstrated that the majority of lizard thymocytes (70%) and only a fraction of lymphocytes in the spleen, peripheral blood and bone marrow were PNA-positive. This positivity was selectively inhibited by galactose as well as lactose, indicating the specificity of binding. Putative PNA receptors were purified from lizard thymocytes and splenocytes by affinity chromatography on a PNA-Sepharose 4B column and resulted in fractions enriched 1,792-fold and 3,141-fold for the PNA-binding component expressed on lizard thymocytes and splenocytes, respectively. Analysis on reducing and non-reducing SDS-PAGE revealed that both thymic and splenic PNA-binding glycoproteins migrated as a single component of 35 KDa, with no evidence for the association into higher multimers in both tissues. Analyses for amino acid and carbohydrate compositions indicated that the thymic and splenic glycoproteins have similar amino acid composition and differed in the content of neutral and amino-sugars as well as sialic acid. The content of the latter residue was relatively higher in the splenic form of the receptor compared to its thymic counterpart, and was inversely correlated with the content of galactosyl residues in both forms of the receptor. The functional significance of PNA-binding glycoproteins during vertebrate evolution is discussed.
Subject(s)
Glycoproteins/metabolism , Lizards/blood , Lymphocytes/metabolism , Receptors, Mitogen/metabolism , Amino Acids/analysis , Animals , Carbohydrates/analysis , Female , Glycoproteins/chemistry , Glycoproteins/isolation & purification , Male , Protein Binding , Receptors, Mitogen/chemistry , Receptors, Mitogen/isolation & purification , Spleen/metabolism , Thymus Gland/metabolismABSTRACT
Two novel lectins that bind selectively to a schistosome-associated fucosyllactose-related determinant have been characterized and purified from the hemolymph of Biomphalaria alexandrina, the snail vector of Schistosoma mansoni. Both lectins were purified by affinity chromatography on a column of equimolar mixture of D- and L-glucose coupled to epoxy-activated Sepharose 6B and sequential elution by D-glucose (designated BaSI) and L-fucose (designated BaSII). Assessment of the structural characteristics, by one- and two-dimensional polyacrylamide gel electrophoresis, indicated that BaSI and BaSII were structurally distinct, and exist in their native forms as multimers of non-covalently associated subunits, that were of different sizes in BaSI and of equal size in BaSII. Removal of N-linked glycans by Endo-beta-N-acetylglucosaminidase F resolved the heterodisperse pattern of BaSI subunits into two spots of 13.2 kDa (pI 7.2) and 10.1 kDa (pI 5.8), and collapsed the acidic charge microheterogeneity of the BaSII subunit into a single spot that corresponded in terms of molecular weight and pI to the basic 13.2-kDa subunit of BaSI. In miracidial binding and inhibition assays with different sugars, both lectins exhibited selectivity towards a fucosyllactose sequence, but BaSII had a higher binding preference to fucose moieties. BaSII-Sepharose 4B affinity chromatography and analysis on two-dimensional gels indicated that multiple copies of the fucosyllactose-related determinant were expressed by heterogeneous, acidic glycoproteins in the miracidial stage of S. mansoni.
Subject(s)
Biomphalaria/immunology , Biomphalaria/metabolism , Lectins/metabolism , Schistosoma mansoni/immunology , Trisaccharides/immunology , Animals , Antigens, Helminth/chemistry , Binding Sites , Carbohydrate Sequence , Glycoproteins/immunology , Helminth Proteins/immunology , Hemolymph/immunology , Molecular Sequence Data , Polysaccharides/chemistry , Polysaccharides/immunology , Schistosoma mansoni/metabolism , Trisaccharides/metabolismABSTRACT
During a period of two years, 24 cases of antrochoanal polyps were diagnosed by clinical examination, nasal endoscopy and computerized tomography. Surgery started with endoscopic transnasal removal of the polyp. Every attempt was made to remove the antral portion of the polyp through the wide ostium. Then transcanine sinuscopy was performed. Remnants of the polyp were detected and removed in five cases. One or more other cysts were found and extirpated in 11 cases. Endoscopic follow-up for 18 months to three years revealed no recurrence. It is recommended that endoscopic middle meatal surgery should be combined with transcanine sinuscopy to ensure complete removal of antrochoanal polyps.
Subject(s)
Nasal Polyps/surgery , Nose/surgery , Adolescent , Adult , Child , Endoscopy , Female , Humans , Male , Maxillary Sinus/pathology , Middle Aged , Nasal Polyps/pathologyABSTRACT
This study compares the induction, hemodynamic, and recovery characteristics of a general anesthetic with desflurane to one with propofol. Sixty outpatients presenting for orthopedic surgery received either a propofol induction of anesthesia followed by desflurane and nitrous oxide (Group 1), a propofol induction followed by propofol infusion and nitrous oxide (Group 2), a desflurane and nitrous oxide induction and maintenance (Group 3), or a desflurane induction and maintenance (Group 4). The quality of induction was inferior in Groups 3 and 4 with more breath-holding and excitation than in Groups 1 and 2. However, there was a more rapid emergence in Group 4 patients than any of the other groups. Group 4 patients were able to say their names (5.6 +/- 2.0 min vs 10.3 +/- 3.3 min, 8.6 +/- 3.1 min, and 9.3 +/- 1.5 min for Groups 1, 2, and 3, respectively) sooner after the discontinuation of anesthesia. Nonetheless, intermediate recovery was similar in Groups 2 and 4 being numerically but not statistically more rapid than in Groups 1 and 3. This pattern of intermediate recovery was also demonstrated by psychomotor function test results. Although there was no difference between the groups in postoperative narcotic requirement, more patients in Group 3 vomited (50%) than in either Group 2 (0%) or Group 4 (12.5%). Hemodynamically, the anesthetics were very similar. Although desflurane was a difficult drug to use for induction of anesthesia, this study demonstrates that desflurane is a suitable maintenance anesthetic for ambulatory surgery because it provides a rapid awakening and an intermediate recovery similar to propofol.