Effect of raising dietary protein, amino acids and/or energy levels as an attempt to alleviate severity of the chronic aflatoxicosis by broiler chicks. 1. Performance and toxicity symptoms.

In an attempt to alleviate the aflatoxicosis symptoms by broiler chicks fed on low (50 ppb) aflatoxin-contaminated diet; the dietary nutrients were elevated by 2.6% for protein, 0.54 MJ ME/kg for energy or 0.5% for each of methionine and lysine. These supplements were mixed altogether and applied as well. All the experimental diets were offered for a 6-week period followed by a 2-week period on aflatoxin--free diet without supplements as a withdrawal period. It was proved after the treatment period that the raising of energy concentration was beneficial to overcome the aflatoxin effects on the body weight, feed conversion rate, and dressing (eviscerated weight) percentage and stability of relative weights of liver and kidneys. Additionally, the supplements' mixture improved the body weight and the relative weight of the liver comparing with the chicks fed on the aflatoxin diet without additives. All of the contaminated diets, except that of the high energy, caused apparently toxic symptoms besides the internal gross pathology. The high energy diet reflected the lowest mortality rate, whereas the highest was with the amino acids supplemented one. The withdrawal period resulted in lower differences among treatments in the body weight. It improved also the dressing percentage (significantly) and to some extent the results of the post-mortem examination.

Effect of raising dietary protein, amino acids and/or energy levels as an attempt to alleviate severity of the chronic aflatoxicosis by broiler chicks. 2. Biochemical characteristics.

Aflatoxin caused some reduction in moisture contents of chest and liver, lipids of thigh and blood, blood glucose, muscular protein and GOT in liver. It led also to increase of moisture contents of thigh and kidneys; chest lipids; blood cholesterol; protein of liver, kidneys and blood and blood creatinine. The different supplements used herein led to increasing moisture of muscles, liver and kidneys (except on oil addition); lipids of muscles (except of chest on high energy diet) and blood (except on amino acids-supplemented diet); blood cholesterol (except on high energy one); protein of thigh (except on high protein one) and blood (except on high energy or amino acids diets) and liver GPT (except on high energy diet). The additives led also to low blood glucose; protein of chest (except on high energy), liver, and kidneys; blood creatinine; liver GOT (particularly with high energy or amino acids); plasma GOT (on high amino acids) and plasma GPT. The 2-week withdrawal period led to low moisture contents of muscles and kidneys of most treatments, although they continued higher than in the control for chest, liver and kidneys. It increased blood glucose and cholesterol with continuous higher lipid content of muscles and blood and blood cholesterol than in the control. It led to elevated protein content of muscles, liver (except on the control or supplements mixture), kidneys (on the aflatoxin alone or with the amino acids) and blood (except on the control or aflatoxin alone or with high protein) and blood creatinine (except on the control or on the high energy or the supplements mixture).(ABSTRACT TRUNCATED AT 250 WORDS)