ABSTRACT
Purposeto describe the clinical characteristics of patients with confirmed SARS-CoV-2 infection in primary care and to analyze the predictive role of different risk factors on prognosis, especially living conditions. MethodsRetrospective longitudinal observational retrospective study by reviewing medical records from a primary care center since March 1 to April 30, 2020. Case definition of confirmed SARS-CoV-2 infection, sociodemographic data, clinical characteristics, comorbidity and living conditions were collected. The statistical analysis consisted in description of the sample, comparison of prognosis groups and analysis of prognostic factors. ResultsA sample of 70 patients with confirmed SARS-CoV-2 infection was obtained, with comorbidity mainly related to arterial hypertension, overweight/obesity, hypercholesterolemia, diabetes and chronic pulmonary pathology. Pneumonia was present in 66%. Exitus occurred in 14% of the sample. Factors associated with mortality were advanced age (84 vs 55; p<0.0001), arterial hypertension (78% vs 41%; p=0.040), asthma-COPD (56% vs 13%; p=0.008) and atrial fibrillation (56% vs 5%; p=0.001). ConclusionsThe study reflects the clinical practice of a primary care center. This kind of studies are essential to strengthen and reorganize the Health System and to try to anticipate the medium- to long-term consequences of COVID-19 on global health.
ABSTRACT
BackgroundReal-time reverse transcription polymerase chain reaction (RT-qPCR) is the current recommended laboratory method to diagnose SARS-CoV-2 acute infection, several factors such as requirement of special equipment, time consuming, high cost and skilled staff limit the use of these molecular techniques. A more rapid and high-throughput method is in growing demand. MethodsEvaluate the performances of the Panbio COVID-19 AG Rapid Test Device (Nasopharyngeal), a rapid immunochromatographic test for the detection of SARS-CoV-2 antigen, in comparison to RT-qPCR. ResultsThe RDT evaluated in this study showed a high sensitivity and specificity in samples mainly obtained during the first week of symptoms and with high viral loads. ConclusionsThis assay seems to be an effective strategy for controlling the COVID-19 pandemic for the rapid identification and isolation of SARS-CoV-2 infected patients.
ABSTRACT
STATEMENT OF PROBLEM: Sodium hypochlorite (NaOCl) may alter resin bond strength by deproteination of demineralized dentin collagen. PURPOSE: To analyze the effect of NaOCl treatment on bond adhesion and tensile strength of different post cementation protocols in vitro. MATERIAL AND METHODS: The periconductual dentin of 120 single-rooted, caries-free, unrestored human teeth was etched with 37% orthophosphoric acid. The teeth in Group I (60) were left untreated, and the teeth in Group II (60) were treated with 10% NaOCl. Four post-cementation protocols were examined (15 teeth in each) for Groups I and II: ED Primer dentin adhesive + Panavia 21 Ex; ED Primer dentin adhesive + Dual Cement; Panavia 21 Ex; and Dual Cement. For each condition, resin tags, hybrid layers over the periconductual dentin and post surface, were analyzed by scanning electron microscopy (SEM). Specimens were loaded with a uniaxial tensile force using an electromechanical testing machine until cement failure. Bond adhesion and tensile strength with different protocols were examined statistically using multiple analysis of variance at a significance level of alpha<.05. RESULTS: SEM revealed morphologic differences; Group II exhibited cylindrical, solid tags and Group I, tapered, hollow tags. Uniaxial tensile strength tests showed that in Group I, Panavia 21 Ex cement was strongest, whereas in Group II, this protocol was the weakest and Dual Cement with adhesive was the strongest. In Group II, Panavia 21 Ex diminished the tensile bond strength; however, combination with dentin adhesive increased it. CONCLUSION: Within the limitations of this study, NaOCl treatment did not significantly alter tensile bond strength, but when combined with the dentin adhesive ED Primer, a significant rise in strength resulted. A positive relationship existed between increased tag numbers and higher tensile bond strength.
