ABSTRACT
A correlation has been shown between a reduced rate of movement of UV-irradiated neuroblastoma cells from G1 into S phase, an essential increase of cells in S phase while progressing through the cell cycle, and a defect in free DNA synthesis on a damaged template. These indices may reflect one and the same cell response to the UV light.
Subject(s)
DNA Repair/radiation effects , DNA, Neoplasm/radiation effects , Mitosis/radiation effects , Neuroblastoma/metabolism , Ultraviolet Rays , Animals , Cell Line , Dose-Response Relationship, Radiation , Flow Cytometry , Interphase/radiation effects , L Cells/cytology , L Cells/radiation effects , Mice , Neuroblastoma/pathology , S Phase/radiation effects , Tumor Cells, Cultured/cytology , Tumor Cells, Cultured/radiation effectsABSTRACT
The postreplication repair (PRR) of DNA has been studied in UV-irradiated (10 J/m2) and carcinogen (7-bromomethylbenz(alpha)anthracene -- BMBA) treated (1.65-3.3) 10(-6) M Chinese hamster clones with different UV-sensitivity. Ultracentrifugation in alkaline sucrose gradients has been used. Compared to the UV-resistent clone V-79, the UV-sensitive clone CHS2 displayed a lower effectivity of PRR of DNA after both UV-irradiation and carcinogen treatment, by 6 and 10 times, resp. Thus, there is a positive correlation between lethal and mutagenic effects of UV-irradiation and the effectivity of PRR of DNA. The same conclusion is apparently true in the case of carcinogen 7-BMBA treated Chinese hamster cells.
