[Using rotation cross-advancement flap for repairing complete unilateral cleft lip and nasolabial deformity].

To evaluate the efficacy of rotation cross-advancement flap method in repairing the nasolabial deformity of complete unilateral cleft lip. A retrospective study was performed to analyze the children who were treated by using the rotating cross-advancement flap for repairing the complete unilateral cleft lip at the Fujian Medical University Union Hospital from October 2018 to July 2019. The clinical data such as patient's lip height, lip length, nostril height, nostril width, nostril area and so on were collected at the pre-operation, post-operation and following-up visits respectively and used to evaluate the efficacy of the treatment. Six children were included in the present study. The ratios of lip height on noncleft side to cleft side were 2∶1 at the pre-operation time. The ratios of nostril height on the noncleft side to the cleft side were about 2∶1. The ratios of the width and the area of the nostril were 1∶2 to 1∶3. At the post-operation time, the ratios of length and height of the lip at the cleft side to the noncleft side were around 1∶1. The shape of the nostrils and nasolabial fold were almost symmetrical between the cleft side and noncleft side. The shapes of the nasal sill were acceptable and the postoperative scars were not obvious. There were no obvious incision healing complications and the treatment effects were satisfactory. Rotation cross-advancement flap method was safe and reliable for repairing the nasolabial deformities in children with complete unilateral cleft lip.

[Morphological classification of mandible posterior region based on cone beam CT images].

Objective: To classify the morphology of mandible posterior region and provide reference for the planning of dental implantation. Methods: Cone beam CT data of 208 patients were collected. The CT data were imported into CS 3D imaging V3 software and then the morphology of mandible posterior region were analyzed. The types of premolar and molar mandible cross-section morphology were recorded, classified and analyzed. Results: The results showed that type A (vertical type) (79%-96%) was the most common in the premolars, whereas type B (inclined type) (36%-37%) and type C (lingual inverted concave) (30%-54%) were the most common types in the molars, followed type D (absorption severe type) (2%-5%). There was a statistically significant differences in tooth positions (P<0.001), tooth deficiency aspect (P<0.001) and different side (P=0.013), different age (P<0.001), and different gender (P=0.007). Conclusions: Using cone beam CT to determine the morphology of mandible may be a reference for the planning of dental implantation.

[Hedgehog pathway antagonist-induced oromandibular limb hypogenesis in mouse].

Objective: To analysis teratogenic effect of GDC-0449 to fetus and set up the animal model of GDC-0449 induced oromandibular limb hypogenesis in mouse for further research of its pathogenesis. Methods: Twenty-seven pregnant Institute of Cancer Research (ICR) mice were randomly divided into: control group, embryonic day 8.5 (E8.5) exposed groups, E9.5 exposed groups, E10.5 exposed groups, E11.5 exposed groups, E12.5 exposed groups, E13.5 exposed groups, E14.5 exposed groups and E15.5 exposed groups. Each group had 3 mice. Exposed groups were treated with the Hedgehog pathway antagonist GDC-0449 at a single dose 150 mg/kg by oral gavage from E8.5 to E15.5. At E16.5, embryonic phenotypes were analyzed in detail by stereo microscope and histology. After establish an optimal dysmorphogenic concentration, 6 pregnant ICR mice were randomly divided into control group and the optimal group, embryonic phenotypes were analyzed by whole-mount skeletal staining and micro-computed tomography at E18.5. Results: The mice were exposed to GDC-0449 on E11.5 and E12.5 had a high incidence of cleft palate. GDC-0449 exposed between E9.5 and E10.5 caused craniofacial and limb dysmorphology, including micrognathia, microglossia, ectrodactylia, partial anodontia and cleft palate. Most interestingly, these are extremely similar to oromandibular limb hypogenesis syndrome. Conclusions: The results of this study indicate that GDC-0449 can be used to induce micrognathia, microglossia, ectrodactylia, partial anodontia and cleft palate. This work established a novel mouse model for oromandibular limb hypogenesis.

[Impact of AFP promoter on the expressions of GFP in tumor cells].

OBJECTIVE: To study the impact of AFP 5'flanking promoter (enhancer) on the expression of GFP in hepatocarcinoma cell. METHODS: Green Fluorescent Protein (GFP) reporter gene expression plasmid pcDNA3-GFP-AFP-w under the direction of AFP 5' flanking promoter (enhancer) was constructed by recombinant DNA technology and confirmed by restriction analyses. pcDNA3-GFP-AFP-w, pcDNA3-GFP and pcDNA3 were transfected into Hela and Bel7402 cells by lipofectin and selected by G418 respectively, after amplification of the positive cell clones, expression of GFP was detected by Western blotting and quantitatively analysed by GEL Doc 2000 digital image systems. RESULTS: The expression of GFP was lower in Bel-GFP-AFP-w than in Bel-GFP but was significantly higher than in Hela-GFP-AFP-w. CONCLUSION: GFP reporter gene plasmid pcDNA3-GFP-AFP-w under the direction of the 3.1 kb AFP 5'flanking promoter (enhancer) can be expressed in HCC Bel7402 cell definitely and specifically.

Hybrid recombination of AcNPV transfer vector with Bombyx mori NPV and expression in the larvae and pupae of silkworm and the stability of the expression product.

Nucleopolyhedrosis virus (NPV) belongs to the group of baculoviruses. The genome of NPV consists of a circular supercoil double stranded DNA approximately 128 kb in length. During the replication of NPV two forms of virus progeny are produced. One is extracellular virus and the other is occluded virus in inclusion body. The occluded virus is embedded within the polyhedron so that the viruses are protected from environmental factors and kept stable for a long time. When the inclusion body is ingested by insects, the polyhedra is dissolved in midgut and under alkaline condition the virus particles are released and the sensitive tissues of the insect are infected. The polyhedrin is encoded by polyhedrin gene of NPV with a molecular weight about 30,000 daltons. The polyhedrin protein is not essential for the infection and replication of NPV. Therefore, the polyhedrin gene can be used as a vector for foreign genes when inserted into NPV DNA and expressing in insect cells or in larvae. The polyhedrin gene is a strong promoter. In general the polyhedrin protein accumulated in the infected cells reaches high levels, about 50% of total cell proteins on SDS-PAGE analysis. The expression product is kept in its natural conformation, and glycosylated. In addition, the baculovirus is not pathogenic to vertebrates and plants. A series of expression vectors of baculovirus developed by Dr. Summers et al. has been used for the expression of a wide variety of heterogenous genes. How to increase the expression efficiency of this system is not quite clear.(ABSTRACT TRUNCATED AT 250 WORDS)