ABSTRACT
The prevention and treatment of coronary heart disease (CHD) is a difficult problem to be solved. More and more studies have found that circular RNAs (circRNAs) may play important roles in the development of CHD. Here detection of vascular smooth muscle cells (VSMCs) showed that circ-SATB2 and STIM1 were up-regulated in proliferative VSMCs, while miR-939 were down-regulated. Circ-SATB2 and miR-939 did not affect the expression of each other, but circ-SATB2 could promote while miR-939 inhibited the expression of STIM1 (a target gene of miR-939). Circ-SATB2 overexpression could inhibit the expression of SM22-alpha (SM22α, a marker of contractile VSMCs), while the expression of SM22α was promoted by miR-939. STIM1 could promote cell proliferation and migration, and circ-SATB2 had similar effects, but its linear sequence had no such functions. MiR-939 had the opposite effects, could promote cell apoptosis and inhibit cell proliferation and migration, and siRNAs targeting circ-SATB2 had similar effects. When co-transfected with circ-SATB2 over-expression vector and miR-939 mimics or STIM1 siRNAs, the changes of cell proliferation, apoptosis and migration were not significant. Therefore, circ-SATB2 can regulate VSMC phenotypic differentiation, proliferation, apoptosis and migration by promoting the expression of STIM1. This discovery will provide a theoretical reference for exploring the role of circRNA in VSMCs and the pathogenesis of CHD.
Subject(s)
Cell Differentiation/genetics , Cell Proliferation/genetics , MicroRNAs/genetics , Myocytes, Smooth Muscle/metabolism , Neoplasm Proteins/genetics , RNA/genetics , Stromal Interaction Molecule 1/genetics , Base Sequence , Cell Line , Cell Movement/genetics , Gene Expression Regulation , Humans , Matrix Attachment Region Binding Proteins/genetics , Microfilament Proteins/genetics , Microfilament Proteins/metabolism , Muscle Proteins/genetics , Muscle Proteins/metabolism , Muscle, Smooth, Vascular/cytology , Neoplasm Proteins/metabolism , RNA Interference , RNA, Circular , Stromal Interaction Molecule 1/metabolism , Transcription Factors/genetics , Up-RegulationABSTRACT
This study investigated the effectiveness of neuromuscular electrical stimulation (NMES) for patients with wrist dysfunction after acute ischemic stroke (AIS).A total of 82 patient cases with wrist dysfunction after AIS were selected in this study. Of these, 41 cases in the intervention group received physical training and NMES treatment. The other 41 cases in the control group received physical training only. The primary outcome was measured by Action Research Arm Test (ARAT) score. The secondary outcomes were measured by the Barthel Index (BI), and numerical rating scale (NRS).After 4-week treatment, patients in the intervention group neither improved arm function recovery, measured by ARAT score (Pâ=â.79), and activities of daily living, measured by BI scale (Pâ=â.62), nor reduced pain, measured by the NRS scale (Pâ=â.11), compared with patients in the control group.The results of this study demonstrated that NMES might not benefit for patients with wrist dysfunction after AIS after 4-week treatment.
