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Nan Fang Yi Ke Da Xue Xue Bao ; 29(5): 856-8, 863, 2009 May.
Article in Chinese | MEDLINE | ID: mdl-19460692

ABSTRACT

OBJECTIVE: To investigate the therapeutic effect of sequential intratumoral injection of xenogeneic antigens in immunized tumor-bearing mice. METHODS: Sequential intratumoral injection of the xenoantigens was performed in immunized mice bearing S180 tumor. The tumor size changes were observed, and the tumor-infiltrating lymphocytes (TIL) including CD3+CD4+T, CD3+CD8+T, and CD3+CD4+CD25+T lymphocytes were counted with flow cytometry. The concentrations of IL-2 and TNF-alpha in the tumor was measured using ELISA. RESULTS: No significant difference was found in the number of CD3+T lymphocytes in the TILs between different groups. After the immunotherapy, the percentages of CD3+CD4+T, CD3+CD8+T and CD3+CD4+CD25+T lymphocytes were 54%, 22% and 2.91%, respectively, with the CD4+/CD8+ ratio of 2.49, significantly different from that in the control group (P<0.05). The concentrations of IL-2 and TNF-alpha were 100.61 pg/ml and 54.114 pg/ml, respectively, significantly different from those in the control group (P<0.05). CONCLUSION: Sequential intratumoral injection of heteragenetic antigena can significantly increase the amount of effector cells and cytokines in the micro-environment of the tumor, and decrease the expression of T regulatory.


Subject(s)
Antigens, Heterophile/immunology , Immunotherapy/methods , Lymphocytes, Tumor-Infiltrating/cytology , Sarcoma 180/therapy , Streptococcus/immunology , Animals , CD4-CD8 Ratio , Female , Male , Mice , Random Allocation , Sarcoma 180/immunology
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