The effect of reduced loading density on pig welfare during long distance transport.

Transport of animals is a stressful procedure often resulting in significant losses for the slaughter plant. This study aimed to determine whether or not pigs would benefit from a loading density (low density (LD)) (179 kg/m2) below the normal EU standard loading density (normal density (ND)) (235 kg/m2). Eight similar, 550-km-long road journeys, were followed in which fattening pigs were transported across Germany from farm to slaughter plant. During each journey all pigs were transported at LD (n=4) or ND (n=4). Twelve female pigs per journey (total n=96) were randomly selected for measurement and monitoring of body temperature, behaviour, heart rate and blood parameters. Throughout the journeys, LD pigs displayed more resting behaviour than ND pigs. Average body temperature was lower (P<0.05) for pigs transported at LD (38.0±0.07°C) than those transported at ND (38.3±0.06°C). During loading heart rate increased in both ND and LD pigs and declined after the vehicle had been closed before departure but remained slightly elevated in ND pigs. Pigs transported at ND displayed signs of stress (elevated HR and body temperatures) during the drivers' break. Blood parameters were only slightly (not significant) effected by loading density. Results indicate that pigs are more capable of adapting to long (550 km) transport conditions when loaded at a density below the present EU requirement.

Risk assessment in animal welfare - especially referring to animal transport.

The increasing awareness of animal welfare in public and science induces a need for objective evaluation. Particularly animal welfare during transport is discussed due to the variable transport conditions in Europe. Therefore an objective evaluation of animal transport is relevant and the first attempts to develop animal welfare risk assessment guidelines for animal transport are made. A report of the development of a guideline on animal welfare risk assessment during animal transport (Dalla Villa et al., 2009) bases on other recent reports and guidelines of animal welfare risk assessment published by EFSA (2009) and adapts the method to animal transport condition. For the implementation of a model for quantitative risk assessment, transport conditions were defined to develop multiple scenarios. Hazard identification was prepared for each scenario and for three selected scenarios a complete risk assessment was performed. Within the exemplary risk assessment potential hazards were initially identified and furthermore hazard description and exposure assessment were evaluated by expert opinion which resulted in risk characterisation. Within risk characterisation of each hazard, magnitude and risk estimate were calculated to afford an individual ranking of these values in order to obtain a graphical illustration of the ten highest estimated risks.

Special problems of long-distance road transports of cattle.

Physiological and behavioural field studies on long distance road transport of 116 heifers, 135 bulls and 64 steers in 10 goose-necked double decked semitrailers from northern Germany to mediteranean ports showed different impacts of handling during pre-transport (i.e. collection of animals, weighing, loading), transport journey itself and post transport handling (i.e. lairage time) on coping strategies of the different categories of cattle. It was found loss of body weight in steers (-6.65%) coming from pasture was higher compared to bulls (-4.6%) during transport, but they recovered during lairage time in a better way. All categories of cattle showed catabolic energy metabolism during transport, but only in bulls and to a farer extent in heifers this leads to a tendency of a ketotic metabolism during second parts of transport and lairage time. During whole transport time no more than 20% of bulls and steers were laying down, and less than 5% were feeding during driving intervals. In all parts of transport general stress parameters like heart rate (with exception to steers) and cortisol were elevated as a part of adaptation to the transport environment, but indicating high physical and emotional loads on the animals with no resting possibilities. In this context animals have to be prepared carefully to be transported, i.e. in reference to energy and fluid balance, and to be feed in sufficient time intervals (breaks) and lengths to maintain fundamental behavioural and physiological needs of the animals during transport. The lairage facilities are very important for the bulls and in the case of heifers the feeding regime during lairage time must be improved to ensure the possibility for a real resting and recovery of the animals after transport.

Recommendations for future development in cattle transport in Europe.

Every year millions of calves and cattle are transported across, from and to Europe. Most of these animals are going to slaughter houses in the respective countries or in another community state or coming or going abroad (extra-EU). These transports give cause for concern for at least three reasons: First, it can cause severe stress in animals entailing poor welfare. Second, stressful transports may have a negative effect on meat quality. Third, there is the risk of spread of infectious diseases over large distances. Existing legislation does not provide enough protection to transported animals especially over long distances largely because considerable parts of the regulations are not sufficiently based on scientific evidence. In recent years some research is carried out including the EU financed CATRA research project (contract QLK5-CT 1999-0157) concentrating on the welfare and meat quality aspects of cattle transport. This paper summarises important results of this recent research and gives some recommendations for future legislation. The welfare of the animals is limited by their needs not by a fixed maximum transport time, if vehicle and transport conditions are appropriate. Bulls, steers and heifers are reacting differently on transport. Adapt transport schemes to the needs of the animals. Meat quality is only effected in extreme situations. Some animals develop an energy deficit after 6 h of transport. Develop appropriate feeding regimes for long transport. Abolish stressful loading and unloading in staging posts (injuries, infectious diseases). Staging posts are particularly stressful for bulls. Educate handlers and drivers more intensively. Pay drivers inverse to losses. Develop monitor systems for long and short distance transport (e.g. records, GPS). Improve vehicle design (e.g. vibration).

Transport stress modulates adrenocorticotropin secretion from peripheral bovine lymphocytes.

The influence of transport stress on the secretion of adrenocorticotropin (ACTH) from peripheral bovine lymphocytes was evaluated by exposing cows to short- (30 min) or long-term (14 h) transport. After transporting animals for 14 h they were given a mandatory rest for 24 h in two different situations, either by off-loading them and allowing them to rest in a stall or by keeping them in the truck. Blood samples were withdrawn before and after transport and after the rest period in long-term transported cows and before and after transport in cows transported for 30 min. Peripheral blood lymphocytes were separated and cultured for 72 h in serum-free medium. Adrenocorticotropin was measured using highly sensitive and specific immunoradiometric assay in culture supernates. We noticed no effect of short-term transport on ACTH secretion from lymphocytes. The ACTH concentration in animals transported for 14 h increased (P < or = 0.01) from 4.72 +/- 0.48 pg x mL(-1)/2 x 10(6) lymphocytes before the transport to 8.24 +/- 1.40 pg x mL(-1) directly after the transport. When animals were off-loaded and rested in a stall for 24 h, ACTH secretion from cultured lymphocytes returned to the basal value of 4.24 +/- 0.31 pg x mL(-1), whereas the animals rested in the truck had ACTH levels of 8.9 +/- 1.43 pg x mL(-1). Phytohemagglutinin, a plant lectin that stimulates lymphocytes, did not affect the lymphocytic ACTH secretion in this study. Heart rate and rectal temperature measured telemetrically increased in cows directly after 14 h of transport but decreased to pretransport values in cows rested for 24 h in cows rested in stalls and those rested inside the truck. This experiment is the first to show lymphocytic ACTH secretion in cows, and the results indicate that ACTH secretion from peripheral lymphocytes could be used as a reliable measurement in stress studies.

[Stress response of slaughter pigs in two different access systems to electrical stunning]. / Belastungsreaktionen von Schlachtschweinen in zwei unterschiedlichen Zutriebssystemen zur elektrobetäubung.

Investigations on two batches of 25 pigs each were carried out to characterize two different access systems for electrical stunning by physiological responses such as heart frequency (HF) and the blood constituents lactate and cortisol. All animals were of the same hybrid breeding program. The access race in system A, where the animals are separated was 11 m long. The personnel used different equipments including electrical rods to move the pigs forward. The lairage time after transport was between 1 to 2 h. In system B the animals arrived the evening before slaughter and spent the night in groups in straw-littered lairage boxes. The access race was 3.5 m long. The heart frequencies of the pigs in system A were between 80 and 240 beats/min, in system B the average HF were between 60 and 170 beats/min. In the lairage the HF were at about 113 beats/min (system A) and 66 beats/min (system B). Blood samples were taken immediately after stunning. The lactate concentrations differed by 6 mmol/l (8.6 mmol/l in system A, 2.6 mmol/l in system B). Cortisol was distinctly higher in the blood of the animals from system A (166 ng/ml) compared to system B (126 ng/ml). The results show that the used techniques are suitable to examine stress indicators of pigs under practical conditions. Access systems with long races seem to pose a higher stress than short access ways. The influence of handling (human factor) and management (size of groups, origin of pigs) is equally important. The role of longer resting times in the lairage should be investigated in more detail. This should also include the keeping conditions on the farm where the animals are raised.