ABSTRACT
OBJECTIVES: This study examined the effects of one-month naringin administration and exercise training on cognitive impairment and H2S signaling pathway in an Amyloid ß (Aß)-injected rat. METHODS: Rats were divided into four groups: control group; rats underwent Aß microinjection surgery, exercise group; rats underwent Aß microinjection surgery and trained by treadmill for four weeks, naringin group; rats underwent Aß microinjection surgery, and rats orally administrated 80 mg.kg-1 naringin for four weeks, naringin+exercise group; rats underwent Aß microinjection surgery and were trained by treadmill for four weeks, and also, rats orally administrated 80 mg.kg-1 naringin for four weeks. After one month of treatment, spatial learning and memory were measured, and then hippocampi were sampled. S-adenosylmethionine (SAM), cystathionine-ß-synthase (CBS), hydrogen sulfide (H2S) levels, and neuronal death were detected in the hippocampi of rats. RESULTS: Naringin and exercise improved spatial learning (latency time, P < .001) and memory (P < .001) in the Morris Water Maze test in Aß-injected rats compared with the control group. SAM (P < .01), CBS (P < .001), and H2S (P < .01) levels are increased in the naringin+exercise group compared with the control group. CONCLUSION: The result of this study supports the effect of exercise and/or naringin to improve cognitive dysfunction and cell death through the production of H2S.
