ABSTRACT
The walls of the third ventricle have been proposed to serve as a bidirectional conduit for exchanges between the neural parenchyma and the cerebrospinal fluid. In immunohistochemical studies of mice, we observed that light exposure and circadian phase affected peptide staining surrounding the third ventricle at the level of the suprachiasmatic nuclei. Under high magnification, we observed robust staining for the neurohormone oxytocin and the calcium-binding protein parvalbumin associated with cilia extending into the third ventricle from the surrounding ventricular wall; no similar staining was observed for vasopressin or calbindin. Retinal illumination had opposite effects on levels of parvalbumin and oxytocin in the cilia: light exposure during late subjective night increased oxytocin staining, but decreased parvalbumin staining in the cilia. Preventing cellular transport with colchicine eliminated immunohistochemical staining for oxytocin in the cilia. There was also a significant daily rhythm of oxytocin immunostaining in the third ventricle wall, and in magnocellular neurons in the anterior hypothalamus. The results suggest that environmental lighting and circadian rhythms regulate levels of oxytocin in the cerebrospinal fluid, possibly by regulating movement of oxytocin through the third ventricle wall.
Subject(s)
Cerebral Ventricles/physiology , Cerebral Ventricles/radiation effects , Circadian Rhythm , Ependyma/physiology , Ependyma/radiation effects , Oxytocin/metabolism , Parvalbumins/metabolism , Animals , Colchicine/pharmacology , Immunohistochemistry , Light , Male , Mice , Mice, Inbred C57BL , Oxytocin/cerebrospinal fluid , Oxytocin/radiation effects , Parvalbumins/radiation effects , Retina/physiology , Retina/radiation effectsABSTRACT
Rats anticipate a scheduled daily meal by entrainment of a circadian pacemaker separate from the light-entrainable circadian pacemaker located in the suprachiasmatic nuclei (SCN). The site and molecular mechanisms of the food-entrainable pacemaker are unknown. The intrinsic period (tau) of the SCN pacemaker is significantly lengthened by deuteriation. Sensitivity of food-entrained circadian rhythms to D(2)O (25% in drinking water) was evaluated in intact and SCN-ablated rats entrained to daily feeding schedules. In intact rats fed ad-libitum, D(2)O lengthened tau sufficiently to drive activity rhythms out of entrainment to the light-dark cycle. By contrast, food-entrained rhythms were surprisingly resistant to modulation by D(2)O. The mean daily onset time of food anticipatory activity in rats with complete SCN-ablations was not affected by up to 28 days of D(2)O intake. Transient delays and disruption of anticipatory activity were evident in intact and one partial SCN-ablated rat during D(2)O treatment, but these are interpretable as effects of coupling and/or masking interactions between a D(2)O-sensitive light-entrainable pacemaker, and a D(2)O-resistant food-entrained pacemaker. Differential sensitivity to D(2)O suggests diversity in the molecular mechanisms of food- and light-entrainable circadian pacemakers in mammals. D(2)O may have utility as a screening test to identify putative food-entrainable pacemakers from among those central and peripheral tissues that can express circadian oscillations of clock genes independent of the SCN.
Subject(s)
Biological Clocks/drug effects , Circadian Rhythm/drug effects , Deuterium Oxide/pharmacology , Drug Resistance/physiology , Eating/drug effects , Suprachiasmatic Nucleus/drug effects , Trans-Activators/drug effects , Animals , Biological Clocks/physiology , CLOCK Proteins , Circadian Rhythm/physiology , Eating/physiology , Food, Formulated , Male , Motor Activity/drug effects , Motor Activity/physiology , Rats , Rats, Sprague-Dawley , Suprachiasmatic Nucleus/injuries , Suprachiasmatic Nucleus/physiology , Trans-Activators/physiologyABSTRACT
Pituitary adenylate cyclase activating polypeptide (PACAP38) is a putative neurochemical of the main retinal input to the mammalian circadian pacemaker housed in the suprachiasmatic nucleus (SCN). We assessed the phase-resetting effects of microinjection of PACAP38 into the SCN region on hamster wheel-running rhythms. When administered during the middle of the subjective day, PACAP38 evoked large but transient phase advances ( approximately 60 min), that were followed by small, steady-state phase delays. During the early subjective night, PACAP38 elicited small to moderate phase delays without any detectable concentration-dependence. Late in the subjective night, PACAP38 had no significant effects. Saline microinjection had no effect at any phase tested. These findings show that PACAP38 has small to moderate effects on the phase of the hamster SCN circadian pacemaker, including significant phase delays early in the subjective night.
Subject(s)
Circadian Rhythm/drug effects , Motor Activity/drug effects , Neuropeptides/pharmacology , Animals , Circadian Rhythm/physiology , Cricetinae , Male , Mesocricetus , Microinjections , Motor Activity/physiology , Pituitary Adenylate Cyclase-Activating Polypeptide , Sodium Chloride/pharmacology , Suprachiasmatic Nucleus/physiologyABSTRACT
FOS protein is synthesized in neuronal nuclei in response to a variety of environmental stimuli and has been used as a marker of stimulus-specific brain function. The present studies were initiated to examine the effects of ultraviolet light on the induction of FOS protein immunoreactivity (FOS-IR) in several brain regions of adult male hamsters. Experiment 1 confirmed previous observations of FOS-IR induced in visual cortex in response to ultraviolet light. However, protein was also induced by ultraviolet or white light in a variety of other areas and induction occurred in both sighted and enucleated animals. Therefore, experiments were conducted to evaluate the effects of a 514 nm light on FOS-IR induction in blind or sighted animals. Experiments 2 and 3 were performed during the early subjective night and mid-subjective day, respectively, using animals about 4 days after bilateral enucleation or sham surgery. In Experiment 2, light and enucleation independently and interactively resulted in increased FOS-IR neuronal nuclei counts. In Experiment 3, there was a main effect of enucleation and an interaction between enucleation and light condition, but no main effect of light. In Experiment 4, conducted during the early subjective night using animals enucleated 60 days earlier, there were neither effects of light or enucleation. The results support the view that, under certain conditions related to subjective time of day and time since enucleation, light can act through unknown extraocular mechanisms to modify brain activity. Further, short term enucleation itself induces widespread alteration in brain function as indicated by increased FOS-IR expression. The results specifically do not support a role for extraretinal photoreception with respect to direct circadian rhythm regulation.
Subject(s)
Blindness/metabolism , Brain/metabolism , Gene Expression Regulation/radiation effects , Nerve Tissue Proteins/biosynthesis , Proto-Oncogene Proteins c-fos/biosynthesis , Animals , Blindness/genetics , Cricetinae , Eye Enucleation , Genes, fos , Light , Male , Mesocricetus , Photic Stimulation , Ultraviolet RaysABSTRACT
Kindling dramatically increases fearful behavior in rats. Because kindling-induced fear increases in magnitude as rats receive more stimulations, kindling provides a superb opportunity to study the nature and neural mechanisms of fear sensitization. Interestingly, these changes in behavior are accompanied by increased binding to inhibitory receptors and decreased binding to excitatory receptors in the CA1 and dentate gyrus regions of the hippocampus. This led us to hypothesize that kindling-induced fear may result from an increased inhibitory tone within hippocampal circuits. To test this hypothesis, we investigated FOS protein immunoreactivity in hippocampal and amygdalar regions of kindled rats that were exposed to an unfamiliar open field. We found that FOS immunoreactivity was significantly decreased in the CA1 region, dentate gyrus, and perirhinal cortex of kindled rats compared to sham-stimulated rats. These results support our hypothesis that kindling-induced fear may be produced by inhibition within hippocampal circuits. They also suggest that neural changes within the hippocampus may be important for the sensitization of fear.
Subject(s)
Fear/physiology , Fear/psychology , Hippocampus/physiology , Kindling, Neurologic/physiology , Amygdala/metabolism , Animals , Behavior, Animal/physiology , Electrodes, Implanted , Hippocampus/metabolism , Immunohistochemistry , Male , Proto-Oncogene Proteins c-fos/metabolism , Rats , Rats, Long-EvansABSTRACT
The clock regulating mammalian circadian rhythmicity resides in the suprachiasmatic nucleus. The intergeniculate leaflet, a major component of the subcortical visual system, has been shown to be essential for certain aspects of circadian rhythm regulation. We now report that midbrain visual nuclei afferent to the intergeniculate leaflet are also components of the hamster circadian rhythm system. Loss of connections between the intergeniculate leaflet and visual midbrain or neurotoxic lesions of pretectum or deep superior colliculus (but not of the superficial superior colliculus) blocked phase shifts of the circadian activity rhythm in response to a benzodiazepine injection during the subjective day. Such damage did not disturb phase response to a novel wheel stimulus. The amount of wheel running or open field locomotion were equivalent in lesioned and control groups after benzodiazepine treatment. Electrical stimulation of the deep superior colliculus, without its own effect on circadian rhythm phase, greatly attenuated light-induced phase shifts. Such stimulation was associated with increased FOS protein immunoreactivity in the suprachiasmatic nucleus. The results show that the circadian rhythm system includes the visual midbrain and distinguishes between mechanisms necessary for phase response to benzodiazepine and those for phase response to locomotion in a novel wheel. The results also refute the idea that benzodiazepine-induced phase shifts are the consequence of induced locomotion. Finally, the data provide the first indication that the visual midbrain can modulate circadian rhythm response to light. A variety of environmental stimuli may gain access to the circadian clock mechanism through subcortical nuclei projecting to the intergeniculate leaflet and, via the final common path of the geniculohypothalamic tract, from the leaflet to the suprachiasmatic nucleus.
Subject(s)
Circadian Rhythm , Visual Pathways/physiology , Animals , Brain/metabolism , Circadian Rhythm/drug effects , Circadian Rhythm/radiation effects , Cricetinae , Darkness , Electric Stimulation , Exploratory Behavior/physiology , Light , Male , Mesocricetus , Motor Activity/physiology , Proto-Oncogene Proteins c-fos/metabolism , Superior Colliculi/physiology , Time Factors , Triazolam/pharmacologyABSTRACT
This study examines the effects of the leptin receptor mutation in obese Zucker rats on entrainment of food-anticipatory rhythms to daily feeding schedules. Leptin is secreted by adipocytes in proportion to fat content, exhibits a daily rhythm in plasma that is synchronized to feeding time, and inhibits activity of arcuate neuropeptide Y neurons that stimulate feeding behavior and regulate metabolism. Activity within this neuropeptide Y system is enhanced by food deprivation and attenuated by overfeeding and diet-induced obesity. Diet-induced obesity, in turn, attenuates food-anticipatory rhythms. If the effects of obesity on food-entrained rhythms are mediated by leptin inhibition of neuropeptide Y neurons, then these rhythms may be enhanced in leptin-insensitive Zucker obese rats. Alternatively, if daily rhythms of leptin mediate the generation or entrainment of these rhythms, Zucker rats may fail to anticipate daily feedings. Zucker obese and lean rats received food for 3 h/day during the midlight period. Both groups exhibited significant food-anticipatory activity that persisted during three cycles of food deprivation, but this rhythm was significantly more robust in obese rats, when expressed as anticipation and persistence ratios, and as peak values. Anticipatory rhythms did not persist in either group when food was provided ad lib. These results indicate that central actions of leptin may mediate the inhibitory effects of obesity on the expression of food-anticipatory rhythms in rats, but do not mediate the inhibitory effects of ad lib food access, and do not serve as necessary internal entrainment cues or clock components for the food-entrainable circadian system.
Subject(s)
Circadian Rhythm/genetics , Circadian Rhythm/physiology , Feeding Behavior/psychology , Obesity/genetics , Obesity/psychology , Animals , Female , Food Deprivation/physiology , Rats , Rats, ZuckerABSTRACT
A variety of observations from several rodent species suggest that a serotonin (5-HT) input to the suprachiasmatic nucleus (SCN) circadian pacemaker may play a role in resetting or entrainment of circadian rhythms by non-photic stimuli such as scheduled wheel running. If 5-HT activity within the SCN is necessary for activity-induced phase shifting, then it should be possible to block or attenuate these phase shifts by reducing 5-HT release or by blocking post-synaptic 5-HT receptors. Animals received one of four serotonergic drugs and were then locked in a novel wheel for 3 h during the mid-rest phase, when novelty-induced activity produces maximal phase advance shifts. Drugs tested at several doses were metergoline (5-HT1/2 antagonist; i.p.), (+)-WAY100135 (5-HT1A postsynaptic antagonist, which may also reduce 5-HT release by an agonist effect at 5-HT1A raphe autoreceptors; i.p.), NAN-190 (5-HT1A postsynaptic antagonist, which also reduces 5-HT release via an agonist effect at 5-HT1A raphe autoreceptors; i.p.) and ritanserin (5-HT2/7 antagonist; i.p. and i.c.v.). Mean and maximal phase shifts to running in novel wheels were not significantly affected by any drug at any dose. These results do not support a hypothesis that 5-HT release or activity at 5HT1, 2 and 7 receptors in the SCN is necessary for the production of activity-induced phase shifts in hamsters.
Subject(s)
Circadian Rhythm/drug effects , Serotonin Antagonists/pharmacology , Animals , Cricetinae , Male , Mesocricetus , Metergoline/pharmacology , Piperazines/pharmacology , Ritanserin/pharmacologyABSTRACT
Wheel running activity can shorten the period (tau) of circadian rhythms in rats and mice. The role of serotonin (5HT), in this effect of behavior on circadian pacemaker function, was assessed by measuring tau during wheel-open and wheel-locked conditions in mice sustaining neurotoxic 5HT lesions directed at the suprachiasmatic nucleus (SCN). Intact mice exhibited a significant lengthening of tau (approximately 10 min) within 3 weeks when running wheels were locked. Mice with immunocytochemically confirmed 5HT depletion showed significantly longer tau than intact mice during wheel access, and did not show a significant change in tau up to 6 weeks after wheels were locked. In these mice, variability of tau across wheel access conditions was similar in magnitude to tau variability in intact mice at two time points without wheel access (+/- 3 min). 5HT-depleted mice also exhibited significantly longer activity periods (alpha), and a significantly delayed peak of activity within alpha. Previous studies show that a delayed peak of activity within alpha is associated with longer tau. Group differences in tau, and apparent failure of wheel-locking to lengthen tau in mice with 5HT lesions, may thus be due to loss of a serotonergic behavioral input pathway to the SCN, or to a lesion-induced change in the waveform of the activity rhythm.
Subject(s)
Behavior, Animal/physiology , Circadian Rhythm/physiology , Feedback/physiology , Serotonin/physiology , 5,7-Dihydroxytryptamine/toxicity , Animals , Immunohistochemistry , Male , Mice , Mice, Inbred C57BL , Motor Activity/drug effects , Serotonin Agents/toxicityABSTRACT
This study investigated the role of the suprachiasmatic nucleus (SCN) circadian pacemaker and its neuropeptide Y (NPY) and serotonin (5-HT) afferents in entrainment (synchronization) of mouse circadian rhythms by treadmill running. Blind C57BL/6j mice were run in treadmills for 3 hr/d for 3-10 weeks after receiving radio-frequency lesions of the SCN or the intergeniculate leaflet (IGL, the source of SCN NPY) or infusions of the 5-HT neurotoxin 5,7-DHT into the SCN area. Of 25 intact mice, 22 entrained and three showed period (tau, the mean duration of the circadian cycle) modulations to scheduled running. Arrhythmic SCN-ablated mice did not synchronize to scheduled running in a way suggestive of circadian pacemaker mediation. Of 15 mice with IGL lesions, only two with partial lesions entrained. Mice with complete IGL lesions (five), confirmed by immunocytochemistry, showed no entrainment or tau changes. Of 19 mice with 5-HT lesions, only two with partial lesions entrained. All but two mice with complete (10) or nearly complete (4) 5-HT denervation, confirmed by immunocytochemistry, showed tau modulations during the treadmill schedule. Failure to entrain was not explained by group differences in tau before the treadmill schedules. The results indicate that the SCN and both NPY and 5-HT are necessary for entrainment to 24 hr schedules of forced running but that complete loss of 5-HT does not prevent modulations of pacemaker motion by behavioral stimuli. Treadmill entrainment in mice may involve synergistic interactions between 5-HT and NPY afferents at some site within the circadian system.
Subject(s)
Circadian Rhythm/physiology , Motor Activity/physiology , Neuropeptide Y/physiology , Serotonin/physiology , Animals , Behavior, Animal/physiology , Geniculate Bodies/physiology , Male , Mice , Mice, Inbred C57BL , Running , Suprachiasmatic Nucleus/physiology , Time FactorsABSTRACT
To characterize properties and mechanisms of non-photic entrainment of circadian rhythms, the effects of scheduled feeding were assessed in intact and suprachiasmatic nuclei (SCN) ablated C57BL/6j mice. During ad libitum food access, mice with no or partial SCN damage exhibited free-running activity and drinking rhythms, whereas mice with complete ablations were arrhythmic. When food was restricted to 4 h/day for 5-9 weeks, intact and partial SCN-ablated mice exhibited anticipatory activity to mealtime, concurrent with free-running rhythms. In some cases, free-running rhythms became entrained to feeding time; this was more prevalent in intact than partial ablated mice and was related to free-running period. Free-running phase or period were modified in other cases, revealing a phase-response profile consistent with other non-photic zeitgebers. Five of 12 mice with complete or near complete SCN ablations showed anticipatory activity. Mice that failed to anticipate were less active generally and sustained larger lesions. Sites of damage unique to non-anticipators were not evident. The results indicate that the SCN is not necessary for anticipatory rhythms in mice, but that cell populations distributed across several hypothalamic areas may be important for at least some behavioral markers of this circadian function.
Subject(s)
Circadian Rhythm/physiology , Feeding Behavior/physiology , Suprachiasmatic Nucleus/physiology , Animals , Mice , Mice, Inbred C57BLABSTRACT
Circadian rhythms in hamsters can be entrained by restricted daily feeding schedules. Phase control may be exerted by feeding per se, or by wheel running in anticipation of food access. Phase modulation by feeding was examined here by depriving hamsters of food for 9-24 h and refeeding at 1 of 7 different zeitgeber times on the first day of constant dim light. Significant group mean phase-advance shifts were observed only following 24 h and 17 h deprivations ending in the mid-subjective day, 7 h before the usual time of lights off (mean shifts 28 min and 66 min, respectively). The largest phase shifts were associated with wheel running during the first 6 h of refeeding. When running wheels were locked during this time in an additional group, no phase shifts were observed. A trend for small phase delays was evident for 14 h deprivations ending at the beginning of the subjective night, but no significant group mean or individual shifts were observed at other refeeding times. Refeeding after food deprivation, thus, appears to have minimal effects on circadian phase in hamsters; wheel running associated with refeeding may account for occasional shifts observed.
Subject(s)
Circadian Rhythm/physiology , Eating/physiology , Feeding Behavior/physiology , Food Deprivation/physiology , Mesocricetus/physiology , Animals , Arousal/physiology , Cricetinae , Male , Motor Activity/physiology , Reinforcement ScheduleABSTRACT
To determine whether sleep deprivation (SD) affects the response of circadian rhythms to light, hamsters were forced to walk on a slowly rotating treadmill for 6 or 24 h, ending early in the night, with or without a light pulse during the last 30 min. SD alone did not produce a significant phase shift. Light pulses (300 and 50 lx) alone induced significant delay shifts (55 and 35 min, respectively). Twenty-four hours SD significantly attenuated the delay to brighter light and 6 h SD significantly attenuated the delay to moderate light. Sleep loss or attendant low-intensity continuous activity appear to modulate the response of the hamster circadian system to light.
Subject(s)
Behavior, Animal/physiology , Circadian Rhythm/physiology , Cricetinae/physiology , Photoperiod , Sleep Deprivation/physiology , Animals , Male , Motor ActivityABSTRACT
This study examined whether the circadian system of rats can serve as a consulted clock for discriminating time of day. Food restricted rats housed in activity wheels were trained to lever press for food in a two-lever T-maze in which the left arm was correct in a morning feeding session, and the right arm in an afternoon session (7 h interval). All six rats learned the task (discrimination ratios > chance on 85-95% of sessions) and exhibited anticipatory wheel running prior to most sessions. Performance was not disrupted by inverting the LD cycle or by omitting 1-3 sessions, indicating that learning was not dependent on light-dark cues, alternation strategies, or physiological states associated with intermeal interval. Five of six additional rats with ablations of the suprachiasmatic nucleus light-entrainable pacemaker acquired the discrimination, indicating that time-of-day cues can be provided by another circadian pacemaker (likely food-entrainable). The results provide the first clear evidence that the circadian system in a mammal can function as a consulted clock that provides discriminative time cues for cognitive processes subserving behavioral plasticity.
Subject(s)
Biological Clocks/physiology , Circadian Rhythm/physiology , Conditioning, Operant/physiology , Discrimination Learning/physiology , Maze Learning/physiology , Suprachiasmatic Nucleus/physiology , Animals , Food Deprivation/physiology , Male , Physical Exertion/physiology , Rats , Rats, Wistar , Reference Values , Running/physiologyABSTRACT
Circadian rhythms in rodents can be phase shifted by appropriately timed activity. This may be dependent on motivational context; running induced by a novel wheel is effective, whereas running induced by cold has been inferred to be ineffective. This issue was reexamined using a different cold exposure procedure. On the first day of constant dark, 6 h before usual dark onset, Syrian hamsters were exposed to cold (+/- 4 degrees C) in their home cages, or were confined to novel wheels for 3 h. Activity rhythms were significantly phase advanced by 92 +/- 10 min following cold exposure and 86 +/- 17 min following novel wheel running, compared to 13 +/- 18 min in a control condition. Most hamsters exhibited eating, drinking, and modest levels of wheel running (1367 +/- 292 counts/6 h) during and for 3 h after cold exposure. Phase shifts following cold were not affected by food and water deprivation but were significantly attenuated by locking the wheel for 6 h beginning at cold onset (24 +/- 12 min). These data indicate that cold-induced running, even at modest levels, is an effective nonphotic Zeitgeber and do not provide support for a hypothesis that motivational contexts determine the phase-shifting value of physical activity.
Subject(s)
Circadian Rhythm/physiology , Motivation , Motor Activity/physiology , Algorithms , Animals , Cold Temperature , Cricetinae , Drinking , Eating , Environment , MesocricetusABSTRACT
Daily schedules of spontaneous, drug-, or novelty-induced running can entrain circadian rhythms in rodents. Forced running, by contrast, has been reported to have weak or no effects, although a thorough comparative study in a single species is lacking. To fill this gap, drinking or activity rhythms were monitored in C57 mice subjected to daily, 3-h bouts of forced treadmill running or to 3-h daily access to home cage running wheels. Entrainment to treadmill running was observed in 17/27 mice, and to restricted wheel access in 11/20 mice. Entrainment was affected by availability of a home cage wheel (e.g., 14/16 mice with no wheel entrained to treadmill running). Phase angle of entrainment was related to prior circadian period (tau), and tau following entrainment exhibited aftereffects. No mice entrained to a 3-h daily schedule of water access, suggesting that entrainment to scheduled running was not related to water or associated food intake. Phase shifts in response to single 3-h bouts of treadmill running or wheel access were small and not reliably induced. The entrainment paradigm is thus recommended for further study of behavioral effects on the mouse circadian system; forced running, in particular, offers several methodological advantages. The results do not support prior suggestions that forced and voluntary activity differ in value as nonphotic zeitgebers.
Subject(s)
Circadian Rhythm/physiology , Physical Exertion/physiology , Animals , Drinking/physiology , Male , Mice , Mice, Inbred C57BL , Motor Activity/physiologyABSTRACT
The effect of morphine on circadial wheel-running rhythms of C57BL/6j mice was examined. Mice received morphine (25 mg kg-1, i.p.) or saline at eight different circadian phases in constant dark. Morphine injections in the middle of the inactive period induced significant advance phase shifts, whereas injections at other times induced small delay shifts or no responses. This phase-response relationship was not altered by optic enucleation. Morphine also induced hyperactivity. Restriction of activity prevented phase shifts. The results indicate that morphine shifts circadian rhythms by its effects on behaviour, rather than by a direct action on the circadian pacemaker. Morphine may represent a useful tool for further study of behaviourally induced phase-resetting in this species.
Subject(s)
Circadian Rhythm/drug effects , Enkephalin, Methionine/agonists , Morphine/pharmacology , Motor Activity/drug effects , Animals , Blindness , Male , Mice , Mice, Inbred C57BL , Reaction Time/drug effects , Running , Videotape RecordingABSTRACT
Rats anticipate a daily meal, provided that meal onset intervals are within a circadian (22-31 hr) range. Food-anticipatory activity (FAA) has been interpreted as evidence for a food-entrained circadian pacemaker or for a computational process that uses stored representations of pacemaker phase. The models make different predictions concerning the symmetry and history dependence of the circadian limits to FAA. To test those predictions, rats were entrained to 24-hr light-dark and feeding cycles and then exposed to feeding cycles of 21, 22, 25, 26, or 27 hr. Rats showed strong FAA to feeding cycles > or = 24 hr, but not to schedules < 24 hr. Prior exposure to long cycles did not promote anticipation under short cycles. Meal omission tests confirmed that failure to observe FAA to < 24-hr feeding cycles was not a result of masking by early mealtimes. These and other aspects of the results are consistent with the entrained oscillator model of FAA.