ABSTRACT
Based on the performed studies, the authors developed test systems to analyze the expression of mRNA of the p53, C-myc, mad1, max, and E2F1 genes. These test systems could reveal a statistically significant difference between follicular adenoma and carcinoma of the thyroid in their expression of p53 mRNA. It should be noted that the use of our developed test systems is promising when searching for the diagnostic and prognostic markers of cancer, analyzing, and creating the genetic networks characterizing this or that cancer.
Subject(s)
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/biosynthesis , Cell Cycle Proteins/biosynthesis , E2F1 Transcription Factor/biosynthesis , Nuclear Proteins/biosynthesis , Proto-Oncogene Proteins c-myc/biosynthesis , Tumor Suppressor Protein p53/biosynthesis , Adenoma/metabolism , Biomarkers, Tumor/biosynthesis , Cell Line, Tumor , Humans , Reagent Kits, Diagnostic , Reverse Transcriptase Polymerase Chain Reaction , Thyroid Neoplasms/metabolismABSTRACT
Demonstrated, that puncture thyroid hystobiopsy is safe and informative method of preoperative thyroid nodes diagnostics. Telomerase activity in tissue samples was significantly higher in case of malignant thyroid disease, although a positive correlation of telomerase activity was also with the amount of lymphocytes in bioptates. Combination of thyroid hystobiopsy with tissue telomerase activity measurement proved to be an effective means of preoperative diagnostic of patients with nodular goiter.
