[Donor age affects on the «behavior¼ and the sensibility bone marrow cells in on copper ion of the primary culture].

The changes of bone marrow cells (BMC) number in the primary culture from 0 to 96 hours, the pattern (the distribution of cells) of cells morphotypes and «lifespan¼ (the time of cell life after isolation) of myelocytes, metamyelocytes, band and segmented neutrophils, isolated of the young (3 months) and old (20months) animals, were investigated. The number of the BMC obtained from intact old animals increased faster in primary culture, than from young animals. The Cu induced fibrosis had different influence on the rate of BMC culture growth of old and young animals. The adding of 4 mM and 8 mM CuSO4x5H2O in the BMC culture of young and old animals resulted in a dose-dependent inhibition of growth rate of young animal cells. If copper ions were added into the culture of BMC of old animals, the decreased of the BMC number was described less than for cells of young animals. The adding of 8 mM CuSO4x5H2O inhibited proliferation less, than the adding of 4 mM CuSO4x5H2O. The Cu-induced liver fibrosis had accelerated the BMC rate death of both old and young animals. However, this effect was more pronounced in young animals. It is suggested, that during the ontogenesis the BMC undergo such epigenetic changes, which change functional properties.

[The T-cell response in parasitization by sheep bot (Oestrus ovis) larvae]. / T-kletochnyi otvet pri parazitirovanii lichinok ovech'ego ovoda Oestrus ovis.

The kinetics of immunocompetent cells in sheep artificially infested with 80, 160, 1000 specimens of Oestrus ovis larvae and immunized with antigen from larvae protein was traced by stableons (E-REC) and antigen-fixing (Ag-REC) rosette formation reaction. It was found that E-REC level changes only a little in both infested and reinfested animals. Ag-REC kinetics mates with the rated larvae biomass changes in all age groups. Ag-REC level of infested animals is much higher and then regularly declines in the following cycles of invasion. T-lymphocytes level and death level of immunized lambs is much higher than in intact animals at the beginning of parasitism.

[Diagnostic value of studies of amino acid metabolism during prolonged contact with pesticides]. / Diagnosticheskoe znachenie issledovaniia aminokislotnogo obmena pri dlitel'nom kontakte s pestitsidami.

Amino acid metabolism was studied experimentally during prolonged administration of a mixture of organochlorine, -metallic, and -phosphorus pesticides and in subjects occupationally exposed to pesticides for a long time. A marked increase of blood serum and liver levels of ser, glu, gly, ala, val, isolei, tyr, phen and a decrease of cystine were detected in white rats. Blood serum serine and threonine hydratases activities increased in parallel with increase of pesticide mixture dose. Reduced levels of taurine, cystine, methionine, alanine and increased levels of phenylalanine were revealed in subjects after prolonged exposure to pesticides. Analysis of blood serum amino acid metabolism parameters may be used as diagnostic criteria of metabolic disorders in a body exposed to pesticides.

[The kinetics of specific serum antibodies in sheep infested with sheep bot larvae]. / Kinetika spetsificheskikh syvorotochnykh antitel u ovets, invazirovannykh lichinikami ovech'ego ovoda.

The kinetics of specific antibodies of the blood serum of sheep experimentally infested with 80, 160 and 1000 specimens of Oestrus ovis larvae was examined. The affinity pure serum IgG and the immunoferment analysis (ELISA) were used for qualitative estimation of specific antibodies. It has been shown that the level of specific antibodies correlates with the larval biomass and is connected with ontogenesis of this parasite. The younger animals, which were infested for the first time, are characterized by more intensive production of specific IgG than adult reinfested ones. The ways of immunity response formation in animals infested with Oestrus ovis larvae are considered.

[The trypsin-antitrypsin system and the content of blood middle-molecular peptides in experimental traumatic shock]. / Tripsin-antitripsinovaia sistema i soderzhanie srednemolekuliarnykh peptidov v krovi pri éksperimental'nom travmaticheskom shoke.

It was established that the activity of trypsin and the content of its inhibitors--alpha 1-inhibitor of proteinases and alpha 2-macroglobulin--undergo phase changes of different meaning and direction with the gradual development of traumatic shock. The character of these changes indicates that trypsinemia is an important pathogenetic link of traumatic shock and corresponds to its severity. The compensatory activation of the trypsin inhibitors is directed to increasing the resistance of the body to trypsinemia. Deficiency of the inhibition system leads to activation of organic proteolysis and growth of the content of middle-molecular peptides in the blood. Such a character of homeostasis disorders substantiates the need for the search for effective measures of correction of the trypsin-antitrypsin system and freeing the organism of the excess of middle-molecular molecules.

[Survival of the larvae of the ship botfly Oestrus ovis L. depending on the function of the immune system of the host's body]. / Vyzhivaemost' lichinok ovech'ego ovoda Oestrus ovis L. v zavisimosti ot sostoianiia immunnoi sistemy organizma khoziaina.

In the experiments on artificial infection the survival of larvae of Oestrus ovis L. in sheep with depressed, normal and stimulated immune system was studied. The maximum number of larvae survived in immune depressed animals (62.9%), the minimum number survived in immune stimulated animals (0.4%). For the estimation of specific immune response the reaction of indirect hemagglutination (IHA), the reaction of diffused precipitation (RDP) and the immune ferment analysis (ELISA) were used.