ABSTRACT
OBJECTIVE: To evaluate changes in ovarian reserve in patients following hysterectomy, with or without bilateral salpingectomy or fimbriectomy. STUDY DESIGN: Open-label, prospective cohort trial of patients undergoing hysterectomy for benign uterine indications. Levels of follicle-stimulating hormone (FSH), anti-Müllerian hormone (AMH), and ultrasonic measures of peak systolic flow velocity/diastolic velocity (S/D) ratio and resistance index (RI) in the ovarian artery were taken from patients before and 6 weeks after hysterectomy, hysterectomy+salpingectomy or hysterectomy+fimbriectomy. RESULTS: The study period was from November 2011 to May 2014. Sixty patients were included in the final analysis, after two patients were lost to follow-up and one patient underwent bilateral oophorectomy. Of these 60 patients, 16 underwent hysterectomy alone (control group), and 44 were included in the study group (22 patients underwent hysterectomy+fimbriectomy and 22 patients underwent hysterectomy+salpingectomy). The mean age of patients was 46 years (standard deviation 4.07 years). Between-group dfferences in FSH, AMH, ovarian volume, ovarian artery S/D ratio and ovarian artery RI were not significant. CONCLUSION: The addition of salpingectomy or fimbriectomy to routine hysterectomy in premenopausal patients does not diminish ovarian reserve.
Subject(s)
Hysterectomy/adverse effects , Ovarian Reserve , Salpingectomy/adverse effects , Adult , Female , Humans , Middle Aged , Premenopause , Prospective StudiesABSTRACT
The histone chaperone FACT plays an important role in facilitating nucleosome assembly and disassembly during transcription. FACT is a heterodimeric complex consisting of Spt16 and SSRP1. The N-terminal domain of Spt16 resembles an inactive aminopeptidase. How this domain contributes to the histone chaperone activity of FACT remains elusive. Here, the crystal structure of the N-terminal domain (NTD) of human Spt16 is reported at a resolution of 1.84â Å. The structure adopts an aminopeptidase-like fold similar to those of the Saccharomyces cerevisiae and Schizosaccharomyces pombe Spt16 NTDs. Isothermal titration calorimetry analyses show that human Spt16 NTD binds histones H3/H4 with low-micromolar affinity, suggesting that Spt16 NTD may contribute to histone binding in the FACT complex. Surface-residue conservation and electrostatic analysis reveal a conserved acidic patch that may be involved in histone binding.
Subject(s)
Cell Cycle Proteins/chemistry , Cell Cycle Proteins/metabolism , Crystallization/methods , Crystallography, X-Ray/methods , Histones/chemistry , Histones/metabolism , Transcription Factors/chemistry , Transcription Factors/metabolism , Histone Chaperones/chemistry , Histone Chaperones/metabolism , Humans , Nucleosomes , Protein Binding , Protein Conformation , Protein Structure, TertiaryABSTRACT
Progressive lung infiltration is a major cause of death in Niemann-Pick disease type A and B (NPA, NPB) and in the recently defined type C2. In type C1 (NPC1), the main manifestations are neurological. We report a patient with a classic, neurological, late infantile form of NPC1 disease, carrying the mutation P474L and the variant I642M in the NPC1 gene, who suffered recurrent respiratory manifestations. Bronchoalveolar lavage of a lung segment due to deteriorating respiratory condition revealed many foamy macrophages and was followed by an improvement in symptoms. Pneumopathy may therefore be considered a feature of NPC1 disease for which a partial bronchoalveolar lavage could be a useful treatment.
Subject(s)
Bronchoalveolar Lavage , Foam Cells/pathology , Lung Diseases/complications , Lung Diseases/therapy , Niemann-Pick Diseases/complications , Adolescent , Carrier Proteins/genetics , Child , Chronic Disease , Humans , Intracellular Signaling Peptides and Proteins , Lung Diseases/pathology , Male , Membrane Glycoproteins/genetics , Mutation , Niemann-Pick C1 Protein , Niemann-Pick Diseases/genetics , Treatment OutcomeABSTRACT
Mineralogical analysis of bronchoalveolar lavage fluid (BALF) by electron microscopy can represent the most suitable method for assessing asbestos exposure. However, it has been claimed that no standardized or systematic approach to the subject of mineralogical analysis exists. This study aimed to evaluate BALF mineralogical analysis by transmission electron microscopy as biomarker of asbestos fiber load. BALF was examined in 108 exposed workers and 57 patients who underwent diagnostic fiberoptic bronchoscopy for various clinical purposes. Asbestos bodies in BALF were counted with a phase-contrast microscope. Fibers were counted and analyzed by transmission electron microscopy, which showed a significant difference between the two populations and positive results for all exposed subjects. Only 82.2% of the exposed population tested positive for asbestos bodies. Subjects with long-term exposure had higher concentrations of fibers than did those with more recent exposure, probably because of the higher workplace exposure levels in the past. The results of the study confirm that fiber concentration in BALF can be considered as a reliable biomarker of past asbestos exposure, even many years after the end of exposure.
Subject(s)
Asbestos/analysis , Bronchoalveolar Lavage Fluid/chemistry , Adult , Aged , Aged, 80 and over , Bronchoscopy , Case-Control Studies , Female , Humans , Italy , Male , Microscopy, Electron , Middle Aged , Occupational Exposure , Statistics, NonparametricSubject(s)
Composite Resins/chemistry , Dental Bonding , Adhesiveness , Bicuspid , Color , Humans , Molar , PorositySubject(s)
Composite Resins , Dental Cavity Preparation , Bicuspid , Dental Bonding , Humans , MolarABSTRACT
The authors consider of trigeminal neurotrophic ulcerations features hypotising relationships with trophic diseases of ora mucosa, creating conditions for development of other oral mucosa diseases (lichen and mycosis).
Subject(s)
Mouth Mucosa/pathology , Trigeminal Nerve/physiopathology , Trigeminal Neuralgia/etiology , Candidiasis, Oral/complications , Humans , Lichen Planus/complications , Mandibular Nerve/physiopathology , Maxillary Nerve/physiopathology , Ophthalmic Nerve/physiopathologyABSTRACT
The AA. discuss how to use some esthetic materials, as the Masking Agents and Tints, considering their technique characteristics and tackling the problem of right dental color remark.
