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1.
RSC Adv ; 9(72): 42524-42532, 2019 Dec 18.
Article in English | MEDLINE | ID: mdl-35542839

ABSTRACT

Ionic liquids are being proposed for the improvement of many refinery-related applications where water and oil coexist. However, the lack of relevant thermodynamic data on equilibrium processes involving water, oil and an ionic liquid is a stumbling block. Phase diagrams of these systems are complex, with many different regions, especially when the ionic liquid is solid at room conditions. This greatly complicates modelling, which is usually neglected or carried out only partially. In this work, for the first time, the simultaneous correlation not only of liquid-liquid and liquid-liquid-liquid but also solid-liquid equilibrium data for ternary systems involving ionic liquids has been carried out. To that end, the ionic liquid 1-hexyl-3-methylpyridinium trifluoromethanesulfonate, with an alkyl chain length that favours nano-segregation, was selected. Phase diagrams with water and different representative oils (octane, toluene and cyclohexane) have been determined at various temperatures and atmospheric pressure. The great capacity of the NRTL model, a powerful tool used in all chemical process simulators, was shown by simultaneously correlating data from all the equilibrium regions. However, adequate equilibrium equations and pivotal strategies were required. Low deviations and a good representation of phase diagrams was achieved. A topological analysis based on the Gibbs common tangent criterion and a stability test allowed validation of the proposed correlation parameters.

2.
Parasite Immunol ; 39(2)2017 Feb.
Article in English | MEDLINE | ID: mdl-27809346

ABSTRACT

Liver flukes represent a paraphyletic group of endoparasitic flatworms that significantly affect man either indirectly due to economic damage on livestock or directly as pathogens. A range of studies have focussed on how these macroscopic organisms can evade the immune system and live inside a hostile environment such as the mammalian liver and bile ducts. Recently, microRNAs, a class of short noncoding gene regulators, have been proposed as likely candidates to play roles in this scenario. MicroRNAs (miRNAs) are key players in development and pathogenicity and are highly conserved between metazoans: identical miRNAs can be found in flatworms and mammalians. Interestingly, miRNAs are enriched in extracellular vesicles (EVs) which are secreted by most cells. EVs constitute an important mode of parasite/host interaction, and recent data illustrate that miRNAs play a vital part. We have demonstrated the presence of miRNAs in the EVs of the trematode species Dicrocoelium dendriticum and Fasciola hepatica (Fhe) and identified potential immune-regulatory miRNAs with targets in the host. After our initial identification of miRNAs expressed by F. hepatica, an assembled genome and additional miRNA data became available. This has enabled us to update the known complement of miRNAs in EVs and speculate on potential immune-regulatory functions that we review here.


Subject(s)
Dicrocoelium/genetics , Dicrocoelium/immunology , Fasciola hepatica/genetics , Fasciola hepatica/immunology , Host-Parasite Interactions/immunology , Immune Evasion/genetics , Immune Evasion/immunology , MicroRNAs/genetics , Animals , Dicrocoeliasis/parasitology , Fascioliasis/parasitology , Humans , Liver/parasitology
3.
Phys Chem Chem Phys ; 18(31): 21610-7, 2016 Aug 03.
Article in English | MEDLINE | ID: mdl-27427420

ABSTRACT

The difficulty in achieving a good thermodynamic description of phase equilibria is finding a model that can be extended to a large variety of chemical families and conditions. This problem worsens in the case of systems containing more than two phases or involving complex compounds such as ionic liquids. However, there are interesting applications that involve multiphasic systems, and the promising features of ionic liquids suggest that they will play an important role in many future processes. In this work, for the first time, the simultaneous correlation of liquid-liquid and liquid-liquid-liquid equilibrium data for ternary systems involving ionic liquids has been carried out. To that end, the phase diagram of the water + [P6 6 6 14][DCA] + hexane system has been determined at 298.15 K and 323.15 K and atmospheric pressure. The importance of this system lies in the possibility of using the surface active ionic liquid to improve surfactant enhanced oil recovery methods. With those and previous measurements, thirteen sets of equilibrium data for water + ionic liquid + oil ternary systems have been correlated. The isoactivity equilibrium condition, using the NRTL model, and some pivotal strategies are proposed to correlate these complex systems. Good agreement has been found between experimental and calculated data in all the regions (one triphasic and two biphasic) of the diagrams. The geometric aspects related to the Gibbs energy of mixing function obtained using the model, together with the minor common tangent plane equilibrium condition, are valuable tools to check the consistency of the obtained correlation results.

4.
Waste Manag ; 48: 285-299, 2016 Feb.
Article in English | MEDLINE | ID: mdl-26603567

ABSTRACT

The thermal decomposition of chrome tanned leather before and after a soaking treatment with NaOH was studied using thermogravimetric analysis (TGA). The effect of the solution concentration (0.2M and 0.5M) and the soaking time (5min and 15min) was evaluated. TGA experiments at four heating rates (5, 10, 15 and 20°Cmin(-1)) were run in a nitrogen atmosphere for every treatment condition. A kinetic model was developed considering the effect of the three variables studied, i.e.: the NaOH solution concentration, the soaking time and the heating rate. The proposed model for chrome tanned leather pyrolysis involves a set of four reactions, i.e.: three independent nth order reactions, yielding the corresponding products and one of them undergoing a successive cero order reaction. The model was successfully applied simultaneously to all the experimental data obtained. The evaluation of the kinetic parameters obtained (activation energy, pre-exponential factor and reaction order) allowed a better understanding of the effect of the alkali treatment on these wastes.


Subject(s)
Textile Industry/methods , Thermogravimetry/methods , Waste Products , Chromium , Kinetics , Models, Theoretical , Sodium Hydroxide/chemistry , Spectroscopy, Fourier Transform Infrared , Waste Management , Waste Products/analysis
5.
Development ; 142(18): 3210-21, 2015 Sep 15.
Article in English | MEDLINE | ID: mdl-26395145

ABSTRACT

During embryo implantation, the blastocyst interacts with and regulates the endometrium, and endometrial fluid secreted by the endometrial epithelium nurtures the embryo. Here, we propose that maternal microRNAs (miRNAs) might act as transcriptomic modifier of the pre-implantation embryo. Microarray profiling revealed that six of 27 specific, maternal miRNAs were differentially expressed in the human endometrial epithelium during the window of implantation--a brief phase of endometrial receptivity to the blastocyst--and were released into the endometrial fluid. Further investigation revealed that hsa-miR-30d, the expression levels of which were most significantly upregulated, was secreted as an exosome-associated molecule. Exosome-associated and free hsa-miR-30d was internalized by mouse embryos via the trophectoderm, resulting in an indirect overexpression of genes encoding for certain molecules involved in the murine embryonic adhesion phenomenon--Itgb3, Itga7 and Cdh5. Indeed, this finding was supported by evidence in vitro: treating murine embryos with miR-30d resulted in a notable increase in embryo adhesion. Our results suggest a model in which maternal endometrial miRNAs act as transcriptomic modifiers of the pre-implantation embryo.


Subject(s)
Blastocyst/metabolism , Embryonic Development/physiology , Endometrium/metabolism , Gene Expression Regulation, Developmental/physiology , MicroRNAs/metabolism , Transcriptome/physiology , Animals , Blotting, Western , Female , Humans , Immunohistochemistry , Mice , Microarray Analysis , Microscopy, Electron, Transmission , Microscopy, Fluorescence , Polymerase Chain Reaction , Transcriptome/genetics
6.
Int J Parasitol ; 45(11): 697-702, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26183562

ABSTRACT

MicroRNAs (miRNAs) are gene regulators that have recently been shown to down-regulate the immune response via extracellular vesicles in the mammalian host of helminthic parasites. Using the miRNA prediction pipeline miRCandRef, we expanded the current miRNA set of the liver fluke Fasciola hepatica (Platyhelminthes, Trematoda) from 16 to 54 miRNAs (42 conserved and 13 novel). Comparing the cellular expression levels with extracellular vesicles, we found all miRNAs expressed and enriched for miRNAs with immuno-regulatory function, tissue growth and cancer. Our findings support the hypothesis that miRNAs are the molecular mediators of the previously demonstrated immune modulatory function of extracellular vesicles.


Subject(s)
Extracellular Vesicles/chemistry , Fasciola hepatica/genetics , MicroRNAs/analysis , MicroRNAs/genetics , Animals , Computational Biology , Gene Expression Regulation , Genomics , Humans , Immunomodulation
7.
Toxicol Rep ; 2: 152-164, 2015.
Article in English | MEDLINE | ID: mdl-28962347

ABSTRACT

The effect of two zeolites, HUSY, NaY and a mesoporous synthesized Al-MCM-41 material on the smoke composition of ten commercial cigarettes brands has been studied. Cigarettes were prepared by mixing the tobacco with the three powdered materials, and the smoke obtained under the ISO conditions was analyzed. Up to 32 compounds were identified and quantified in the gas fraction and 80 in the total particulate matter (TPM) condensed in the cigarettes filters and in the traps located after the mouth end of the cigarettes. Al-MCM-41 is by far the best additive, providing the highest reductions of the yield for most compounds and brands analyzed. A positive correlation was observed among the TPM and nicotine yields with the reduction obtained in nicotine, CO, and most compounds with the three additives. The amount of ashes in additive free basis increases due to the coke deposited on the solids, especially with Al-MCM-41. Nicotine is reduced with Al-MCM-41 by an average of 34.4% for the brands studied (49.5% for the brand where the major reduction was obtained and 18.5 for the brand behaving the worst). CO is reduced by an average of 18.6% (ranging from 10.3 to 35.2% in the different brands).

8.
Trans R Soc Trop Med Hyg ; 108(12): 774-82, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25344696

ABSTRACT

BACKGROUND: The present study was carried out to assess the prevalence of intestinal parasite infections among children in Department of Rio San Juan (Nicaragua), to explore the extent of polyparasitism, and to identify the risk factors that might favour transmission of these parasites. METHODS: A coprological study of single stool specimen, collected at random from 382 children (167 boys and 215 girls) aged 2-15 years from two municipalities, and a questionnaire, concerning demographic and epidemiological data on sanitary and behavioural habits, was filled in to identify risk factors. RESULTS: About 93% of the children presented infection. Blastocystis hominis, the most frequently protozoa, and Trichuris trichiura, Ascaris lumbricoides and hookworm, the most prevalent helminths. Polyparasitism (85.4%[326/382]), with the highest percentages detected in females (p=0.001), in the 6-11 year age group (p<0.001) and having a rural background (p<0.001). A firm relationship between any parasite and age (OR=1.2, p=0.036) and rural background (OR=0.3, p=0.027) was detected. Living in rural areas and drinking river water were also positively associated with each of the STH species detected, and walking barefoot was also associated with hookworm infection. CONCLUSIONS: Government efforts should be focused on controlling the risk factors associated with these enteroparasites, with health education programmes in rural areas of Nicaragua.


Subject(s)
Feces/parasitology , Intestinal Diseases, Parasitic/epidemiology , Adolescent , Ancylostomatoidea/parasitology , Animals , Ascaris lumbricoides/parasitology , Child , Child, Preschool , Cross-Sectional Studies , Female , Humans , Male , Nicaragua/epidemiology , Parasites/classification , Parasites/isolation & purification , Risk Factors , Rural Population , Sanitation/statistics & numerical data , Trichuris/parasitology , Water Supply/standards
9.
Toxicol Rep ; 1: 122-136, 2014.
Article in English | MEDLINE | ID: mdl-28962233

ABSTRACT

In this study 11 commercial roll-your-own (RYO) tobacco brands sold in Spain and the reference tobacco 3R4F have been smoked and several components of the mainstream tobacco smoke have been analyzed. Cigarettes were prepared using commercial tubes, and were smoked under smoking conditions based on the ISO 3308. The gaseous and condensed fractions of the smoke from RYO brands and 3R4F have been analyzed and compared. RYO tobaccos, as opposed to 3R4F, present lower amounts of condensed products in the traps than in the filters. In general, RYO tobaccos also provide lower yields of most of the compounds detected in the gas fraction. The yield of CO is between 15.4 and 20.4 mg/cigarette. In most of the cases studied, RYO tobaccos deliver higher amounts of nicotine than the 3R4F tobacco. On average, the yield of the different chemical families of compounds appearing in the particulate matter retained in the cigarette filters tends to be around three times higher than those obtained from 3R4F, whereas similar values have been obtained in the particulate matter retained in the traps located after the filters. It can be concluded that RYO tobaccos are not less hazardous than the reference tobacco, which may be contrary to popular belief.

10.
Bioresour Technol ; 131: 86-93, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23340106

ABSTRACT

Microalgae have many applications, such as biodiesel production or food supplement. Depending on the application, the optimization of certain fractions of the biochemical composition (proteins, carbohydrates and lipids) is required. Therefore, samples obtained in different culture conditions must be analyzed in order to compare the content of such fractions. Nevertheless, traditional methods necessitate lengthy analytical procedures with prolonged sample turn-around times. Results of the biochemical composition of Nannochloropsis oculata samples with different protein, carbohydrate and lipid contents obtained by conventional analytical methods have been compared to those obtained by thermogravimetry (TGA) and a Pyroprobe device connected to a gas chromatograph with mass spectrometer detector (Py-GC/MS), showing a clear correlation. These results suggest a potential applicability of these techniques as fast and easy methods to qualitatively compare the biochemical composition of microalgal samples.


Subject(s)
Biopolymers/analysis , Biopolymers/chemistry , Gas Chromatography-Mass Spectrometry/methods , Heating/methods , Stramenopiles/metabolism , Thermogravimetry/methods , Species Specificity , Stramenopiles/classification
11.
Ann Hematol ; 92(1): 19-24, 2013 Jan.
Article in English | MEDLINE | ID: mdl-22948274

ABSTRACT

Morphology is the basis of the diagnosis of myelodysplastic syndromes (MDS). The WHO classification offers prognostic information and helps with the treatment decisions. However, morphological changes are subject to potential inter-observer variance. The aim of our study was to explore the reliability of the 2008 WHO classification of MDS, reviewing 100 samples previously diagnosed with MDS using the 2001 WHO criteria. Specimens were collected from 10 hospitals and were evaluated by 10 morphologists, working in five pairs. Each observer evaluated 20 samples, and each sample was analyzed independently by two morphologists. The second observer was blinded to the clinical and laboratory data, except for the peripheral blood (PB) counts. Nineteen cases were considered as unclassified MDS (MDS-U) by the 2001 WHO classification, but only three remained as MDS-U by the 2008 WHO proposal. Discordance was observed in 26 of the 95 samples considered suitable (27 %). Although there were a high number of observers taking part, the rate of discordance was quite similar among the five pairs. The inter-observer concordance was very good regarding refractory anemia with excess blasts type 1 (RAEB-1) (10 of 12 cases, 84 %), RAEB-2 (nine of 10 cases, 90 %), and also good regarding refractory cytopenia with multilineage dysplasia (37 of 50 cases, 74 %). However, the categories with unilineage dysplasia were not reproducible in most of the cases. The rate of concordance with refractory cytopenia with unilineage dysplasia was 40 % (two of five cases) and 25 % with RA with ring sideroblasts (two of eight). Our results show that the 2008 WHO classification gives a more accurate stratification of MDS but also illustrates the difficulty in diagnosing MDS with unilineage dysplasia.


Subject(s)
Bone Marrow Examination , Bone Marrow/pathology , Myelodysplastic Syndromes/diagnosis , Observer Variation , Anemia, Refractory, with Excess of Blasts/diagnosis , Anemia, Refractory, with Excess of Blasts/pathology , Biopsy , Cell Lineage , Chromosome Aberrations , Cytogenetic Analysis , Hematology , Humans , Laboratories, Hospital , Laboratory Proficiency Testing , Myelodysplastic Syndromes/classification , Myelodysplastic Syndromes/pathology , Reproducibility of Results , Single-Blind Method , Spain , World Health Organization
12.
Bioresour Technol ; 119: 1-6, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22728174

ABSTRACT

Nannochloropsis oculata was grown in an outdoor bubble column photobioreactor. To obtain information about the behaviour of microalgae/photobioreactor system related to the CO(2) net balance, an analysis of the pH profiles during microalgae growth was carried out. The use of the carbonate equilibrium chemistry and the overall CO(2) volumetric mass transfer in the photobioreactor has permitted to obtain information of the CO(2) losses/CO(2) microalgae consumption ratios. The simplicity of the technique used (a pH probe) could extend the use of this methodology for the correct selection of the photobioreactor/microalgae parameters with the aim to maximize the [CO(2) uptaken/(CO(2) uptaken+CO(2) stripped)] ratios.


Subject(s)
Biological Assay/instrumentation , Carbon Dioxide/chemistry , Carbon Dioxide/metabolism , Culture Media/chemistry , Microalgae/physiology , Photobioreactors/microbiology , Carbon Dioxide/analysis , Cell Culture Techniques , Culture Media/analysis , Equipment Design , Equipment Failure Analysis , Hydrogen-Ion Concentration , Light , Metabolic Clearance Rate , Microalgae/radiation effects
13.
Food Chem Toxicol ; 50(5): 1317-33, 2012 May.
Article in English | MEDLINE | ID: mdl-22342527

ABSTRACT

In this study 10 commercial top selling cigarette brands in Spain have been machine smoked and the mainstream smoke has been analyzed. Multifunctional analysis has been satisfactorily employed to correlate the effect of the main design features of the cigarettes including amount of tobacco, filter size or paper weight with the amount of smoked tobacco and with the ratio CO(2)/CO. The composition of the vapor phase and that of the particulate matter have been analyzed. The particulate matter retained in the filter of the cigarettes has also been analyzed showing a distinct behavior of the compounds condensed in the cigarette filters and in the traps, related to their retention time. A general trend for the relative yield of some compounds in the different brands have been identified and confirmed by multifunctional analysis. Nevertheless, there are some noticeable compounds that behave differently in the different brands.


Subject(s)
Nicotiana/chemistry , Smoke/analysis , Gas Chromatography-Mass Spectrometry , Spain
14.
Exp Parasitol ; 129(3): 307-11, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21875583

ABSTRACT

In order to investigate the factors determining the expulsion of intestinal helminths, we have analyzed the in vivo expression of IL-17, TGF-ß and IL-23 in several tissues of two host species displaying different compatibility with Echinostoma caproni (Trematoda). We did not observe upregulation of these cytokines in any of the tissues of the high compatible host (mice). In contrast, the responses in the host of low compatibility (rats) with the parasite were markedly different. Significant increases in the expression of IL-17 and TGF-ß were observed in the Peyer's patches and the intestine from the 2 to 8 weeks post-infection. The expression of IL-23 was upregulated from 2 to 4 weeks post-infection in the spleen, Peyer's patches and the intestine. Considering together our results with those published previously the development of chronic infections appears to be related with the development of local Th1 responses, whereas the early rejection of the worms is mediated by the development a biased Th17/Th2 phenotype. The Th17 response generated in rats may facilitate the worm expulsion via the suppression of the inflammatory Th1 responses and the increase in intestinal contractility.


Subject(s)
Echinostoma/immunology , Echinostomiasis/immunology , Th17 Cells/immunology , Animals , Ileum/immunology , Interleukin-17/biosynthesis , Interleukin-17/genetics , Interleukin-23/biosynthesis , Interleukin-23/genetics , Lymph Nodes/immunology , Male , Mice , Mice, Inbred ICR , Peyer's Patches/immunology , RNA, Messenger/metabolism , Rats , Rats, Wistar , Spleen/immunology , Transforming Growth Factor beta/biosynthesis , Transforming Growth Factor beta/genetics
15.
Trop Biomed ; 28(1): 40-7, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21602767

ABSTRACT

A cross-sectional study of cryptosporidiosis was carried out in Nicaragua. The prevalence of Cryptosporidium infection was determined in 272 (110 boys and 162 girls) schoolchildren, aged between 4 to 15 years from department of Rio San Juan. The total percentage obtained for Cryptosporidium (35.7%) was one of the highest reported so far. Cryptosporidium appeared in 94.8% of multiparasitism cases. No significant statistical differences were detected in the cryptosporidiosis prevalence between loose/watery (22.2%) and soft/formed (36.7%) stool samples. No significant age and sex differences were observed. This is the first report to identify Cryptosporidium in Nicaragua at species level, providing a preliminary molecular characterization of all positive samples, such as Cryptosporidium parvum (genotype 2). The high prevalence of C. parvum suggests that animals may be potential sources of infection for human cryptosporidiosis, although C. parvum infections may have originated from humans themselves. The human health problem caused by Cryptosporidium in this region may be related to the poor human hygiene/sanitation and contamination of the environment, food, or water supplies. Continuous exposure to the parasite could have been protective against development of symptoms in the children examined.


Subject(s)
Cryptosporidiosis/epidemiology , Cryptosporidium/genetics , Cryptosporidium/isolation & purification , Adolescent , Animals , Child , Child, Preschool , Cross-Sectional Studies , Cryptosporidiosis/parasitology , Cryptosporidium/classification , Feces/parasitology , Female , Humans , Male , Nicaragua/epidemiology , Prevalence , Schools , Students
16.
Environ Geochem Health ; 32(6): 491-515, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20480386

ABSTRACT

Arsenic (As) speciation in surface and groundwater from two provinces in Argentina (San Juan and La Pampa) was investigated using solid phase extraction (SPE) cartridge methodology with comparison to total arsenic concentrations. A third province, Río Negro, was used as a control to the study. Strong cation exchange (SCX) and strong anion exchange (SAX) cartridges were utilised in series for the separation and preservation of arsenite (As(III)), arsenate (As(V)), monomethylarsonic acid (MA(V)) and dimethylarsinic acid (DMA(V)). Samples were collected from a range of water outlets (rivers/streams, wells, untreated domestic taps, well water treatment works) to assess the relationship between total arsenic and arsenic species, water type and water parameters (pH, conductivity and total dissolved solids, TDS). Analysis of the waters for arsenic (total and species) was performed by inductively coupled plasma mass spectrometry (ICP-MS) in collision cell mode. Total arsenic concentrations in the surface and groundwater from Encon and the San José de Jáchal region of San Juan (north-west Argentina within the Cuyo region) ranged from 9 to 357 µg l(-1) As. Groundwater from Eduardo Castex (EC) and Ingeniero Luiggi (LU) in La Pampa (central Argentina within the Chaco-Pampean Plain) ranged from 3 to 1326 µg l(-1) As. The pH range for the provinces of San Juan (7.2-9.7) and La Pampa (7.0-9.9) are in agreement with other published literature. The highest total arsenic concentrations were found in La Pampa well waters (both rural farms and pre-treated urban sources), particularly where there was high pH (typically > 8.2), conductivity (>2,600 µS cm(-1)) and TDS (>1,400 mg l(-1)). Reverse osmosis (RO) treatment of well waters in La Pampa for domestic drinking water in EC and LU significantly reduced total arsenic concentrations from a range of 216-224 µg l(-1) As to 0.3-0.8 µg l(-1) As. Arsenic species for both provinces were predominantly As(III) and As(V). As(III) and As(V) concentrations in San Juan ranged from 4-138 µg l(-1) to <0.02-22 µg l(-1) for surface waters (in the San José de Jáchal region) and 23-346 µg l(-1) and 0.04-76 µg l(-1) for groundwater, respectively. This translates to a relative As(III) abundance of 69-100% of the total arsenic in surface waters and 32-100% in groundwater. This is unexpected because it is typically thought that in oxidising conditions (surface waters), the dominant arsenic species is As(V). However, data from the SPE methodology suggests that As(III) is the prevalent species in San Juan, indicating a greater influence from reductive processes. La Pampa groundwater had As(III) and As(V) concentrations of 5-1,332 µg l(-1) and 0.09-592 µg l(-1) for EC and 32-242 µg l(-1) and 30-277 µg l(-1) As for LU, respectively. Detectable levels of MA(V) were reported in both provinces up to a concentration of 79 µg l(-1) (equating to up to 33% of the total arsenic). Previously published literature has focused primarily on the inorganic arsenic species, however this study highlights the potentially significant concentrations of organoarsenicals present in natural waters. The potential for separating and preserving individual arsenic species in the field to avoid transformation during transport to the laboratory, enabling an accurate assessment of in situ arsenic speciation in water supplies is discussed.


Subject(s)
Arsenic/analysis , Environmental Monitoring , Water Pollutants, Chemical/analysis , Water/chemistry , Argentina , Arsenic/chemistry , Mass Spectrometry , Solid Phase Extraction , Water Pollutants, Chemical/chemistry , Water Supply
17.
Environ Geochem Health ; 32(6): 479-90, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20490622

ABSTRACT

A field method is reported for the speciation of arsenic in water samples that is simple, rapid, safe to use beyond laboratory environments, and cost effective. The method utilises solid-phase extraction cartridges (SPE) in series for selective retention of arsenic species, followed by elution and measurement of eluted fractions by inductively coupled plasma mass spectrometry (ICP-MS) for "total" arsenic. The method is suitable for on-site separation and preservation of arsenic species from water. Mean percentage accuracies (n = 25) for synthetic solutions of arsenite (As(III)), arsenate (As(V)), monomethylarsonic acid (MA), and dimethylarsinic acid (DMA) containing 10 µg l(-1) As, were 98, 101, 94, and 105%, respectively. Data are presented to demonstrate the effect of pH and competing anions on the retention of the arsenic species. The cartridges were tested in the UK and Argentina at sites where arsenic was known to be present in surface and groundwaters, respectively, at elevated concentrations and under challenging matrix conditions. In Argentinean groundwater, 4-20% of speciated arsenic was present as MA and 20-73% as As(III). In UK surface waters, speciated arsenic was measured as 7-49% MA and 12-42% DMA. Comparative data from the field method using SPE cartridges and the laboratory method using liquid chromatography coupled to ICP-MS for all water samples provided a correlation of greater than 0.999 for As(III) and DMA, 0.991 for MA, and 0.982 for As(V) (P < 0.01).


Subject(s)
Arsenic/analysis , Environmental Monitoring/methods , Water Pollutants, Chemical/analysis , Water/chemistry , Argentina , Arsenic/chemistry , Environmental Monitoring/instrumentation , Ion Exchange , Mass Spectrometry , Solid Phase Extraction , United Kingdom , Water Pollutants, Chemical/chemistry
18.
Parasitology ; 137(10): 1577-83, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20388238

ABSTRACT

Strongyloidiasis can be perpetuated by autoinfection with the filariform larvae L3, causing asymptomatic chronic infections and creating a population of carriers, affecting not only developing countries. So far, very little is known about the proteins that interact with the human host, and few proteins from the infective Strongyloides stercoralis L3 have been characterized. Here, we report results obtained from a proteomic analysis of the proteins from S. stercoralis L3 larvae obtained from patients. Since the genome of S. stercoralis is not yet available, we used proteomic analysis to identify 26 different proteins, 13 of them released by short digestion with trypsin, which could represent surface-associated proteins. The present work extends our knowledge of host-parasite interactions by identifying proteins that could be of interest in the development of diagnostic tools, vaccines, or treatments for a neglected disease like strongyloidiasis.


Subject(s)
Helminth Proteins/metabolism , Host-Parasite Interactions , Proteomics/methods , Strongyloides stercoralis/physiology , Strongyloidiasis/parasitology , Animals , Chronic Disease , Feces/parasitology , Gas Chromatography-Mass Spectrometry , Helminth Proteins/chemistry , Helminth Proteins/genetics , Humans , Larva/physiology , Spain , Strongyloides stercoralis/metabolism
19.
Parasitology ; 135(12): 1469-77, 2008 Oct.
Article in English | MEDLINE | ID: mdl-18752710

ABSTRACT

We cloned and expressed Echinostoma caproni HSP70 in Escherichia coli. This molecule presents an open reading frame (ORF) of 655 amino acids, and a theoretical molecular weight of 71 kDa. E. caproni HSP70 protein showed a high homology to other helminth molecules, major differences being located in the C-terminal region of the molecule, with a hydrophobic portion. Studies of protein and messenger RNA (mRNA) expression revealed a distinct pattern, depending on the host (low- or high-compatible). Specific polyclonal antisera raised against the recombinant protein expressed in Escherichia coli demonstrated its selective presence in excretory/secretory products (ESP) of adult parasites obtained from high-compatible hosts. Immunological studies showed clearly the association of HSP70 with the parasite surface and other structures, including eggs.


Subject(s)
Echinostoma/genetics , Echinostoma/metabolism , HSP70 Heat-Shock Proteins/genetics , HSP70 Heat-Shock Proteins/metabolism , Amino Acid Sequence , Animals , Cloning, Molecular , Cricetinae , Feces/parasitology , Gene Expression Regulation , HSP70 Heat-Shock Proteins/chemistry , Host-Parasite Interactions , Immunohistochemistry , Male , Mesocricetus , Molecular Sequence Data , Parasite Egg Count , Rats , Rats, Wistar
20.
Parasite Immunol ; 30(5): 271-9, 2008 May.
Article in English | MEDLINE | ID: mdl-18221450

ABSTRACT

Antigenic proteins of Echinostoma caproni (Trematoda) against mouse IgM, IgA, IgG, IgG1 and IgG2a were investigated by immunoproteomics. Excretory/secretory products (ESP) of E. caproni separated by two-dimensional (2D) gel electrophoresis were transferred to nitrocellulose membranes and probed with the different mouse immunoglobulin classes. A total of four proteins (enolase, 70 kDa heat-shock protein (HSP-70), actin and aldolase) were accurately identified. Enolase was recognized in eight different spots of which seven of them were detected in the expected molecular weight and were recognized by IgA, IgG or IgG and IgG1. Another spot identified as enolase at 72 kDa was only recognized by IgM. Digestion with N-glycosidase F of the 72 kDa band rendered a polypeptide with an apparent molecular weight similar to that expected for enolase recognized by Western immunoblotting using anti-enolase antibodies. This suggests that glycosylated forms of enolase may be involved in the early thymus-independent responses against E. caproni. Early IgM responses were also generated by actin and the HSP-70 which suggests that these proteins are exposed early to the host and may be of importance in the parasite establishment. The IgA responses also appear to be mediated by the HSP-70 and aldolase which could be related with the close contact of these proteins with the host mucosal surface after secretion.


Subject(s)
Antigens, Helminth/isolation & purification , Echinostoma/immunology , Echinostomiasis/immunology , Immunoglobulins/chemistry , Proteomics/methods , Actins/isolation & purification , Animals , Blotting, Western , Electrophoresis, Gel, Two-Dimensional , Fructose-Bisphosphate Aldolase/isolation & purification , HSP70 Heat-Shock Proteins/isolation & purification , Immunoglobulins/immunology , Male , Mice , Mice, Inbred ICR , Phosphopyruvate Hydratase/isolation & purification , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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