Contribution of Primary Biological Aerosol Particles to airborne particulate matter in indoor and outdoor environments.

The atmospheric concentration of bioparticles was determined in some outdoor and indoor sites by using a commercial low-volume sampler and a detection method based on particle collection on polycarbonate filters, propidium iodide staining, observation by fluorescence microscopy and image analysis. Outdoor sampling was continuously carried out from May 2015 to October 2016 by cumulating monthly samples over individual filters. PBAPs contribution to PM10 concentration was in the range 0.7-13%. Seasonal differences were found in PBAPs concentration, shape and mass distribution. Higher concentrations were recorded during the warm period, when the bioparticles were more numerous, larger and more elongated. Simultaneous indoor and outdoor daily samples were collected during the spring of 2014 and 2017 in domestic environments. In indoor sites PBAPs were much higher in concentration than outdoors and showed a different visual appearance, with very wide polyhedral-shaped particles identifiable as skin flakes. Indoor/outdoor ratio (I/O) of PBAPs was in the range 6-16. Indoors, PBAPs contributed 21-77% to organic matter and 16-68% to PM10. When sampling into a sealed room, I/O was only 0.01 for individual bioparticles heavier than100 ng, while it was in the range 0.24-0.43 for PBAPs below 20 ng. This suggests that the infiltration factor of wide bioparticles was very low and that their concentration increase in indoor environments was due to indoor sources, namely the presence of human beings. Samplings carried out in different rooms of an apartment showed that most of the PBAPs mass was due to particles heavier than 100 ng, particularly in the bedroom.

Evaluating a filtering and recirculating system to reduce dust drift in simulated sowing of dressed seed and abraded dust particle characteristics.

BACKGROUND: The pneumatic precision drills used in maize sowing can release dust owing to abrasion of dressed seed; the drift of dust containing insecticide active ingredients is harmful to honey bees. Therefore, we developed a device for drills that uses partial recirculation and filtration of the air by means of an antipollen and an electrostatic filter. RESULTS: Tests were carried out by simulating the sowing of seed treated with imidacloprid, thiamethoxam, clothianidin and fipronil. Dust released by the drill in different configurations was analysed to assess its mass and active ingredient concentration, size distribution and particle number concentration. In general, particles with a diameter smaller than 2.5 and 10 µm represent about 40 and 75% of the total dust mass respectively. The finest size fraction (<1 µm) contains a higher content of active ingredient. The prototype equipped with both antipollen and electrostatic filters always showed a reduction in dust emission greater than 90% in terms of both total mass and active ingredient amount, with a greater efficiency in the reduction in particles below 4 µm. CONCLUSION: This study presents an engineering solution addressing dust losses during sowing, contributes to the description of abrasion dust fractions and provides suggestions for further development of the prototype. © 2016 Society of Chemical Industry.

A new method for assessing the contribution of Primary Biological Atmospheric Particles to the mass concentration of the atmospheric aerosol.

Primary Biologic Atmospheric Particles (PBAPs) constitute an interesting and poorly investigated component of the atmospheric aerosol. We have developed and validated a method for evaluating the contribution of overall PBAPs to the mass concentration of atmospheric particulate matter (PM). The method is based on PM sampling on polycarbonate filters, staining of the collected particles with propidium iodide, observation at epifluorescence microscope and calculation of the bioaerosol mass using a digital image analysis software. The method has been also adapted to the observation and quantification of size-segregated aerosol samples collected by multi-stage impactors. Each step of the procedure has been individually validated. The relative repeatability of the method, calculated on 10 pairs of atmospheric PM samples collected side-by-side, was 16%. The method has been applied to real atmospheric samples collected in the vicinity of Rome, Italy. Size distribution measurements revealed that PBAPs was mainly in the coarse fraction of PM, with maxima in the range 5.6-10 µm. 24-h samples collected during different period of the year have shown that the concentration of bioaerosol was in the range 0.18-5.3 µg m(-3) (N=20), with a contribution to the organic matter in PM10 in the range 0.5-31% and to the total mass concentration of PM10 in the range 0.3-18%. The possibility to determine the concentration of total PBAPs in PM opens up interesting perspectives in terms of studying the health effects of these components and of increasing our knowledge about the composition of the organic fraction of the atmospheric aerosol.