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1.
Article in English | MEDLINE | ID: mdl-37998308

ABSTRACT

Firefighters face many hazards on the job and have a high rate of work-related injuries and illnesses (WRII). We analyzed Washington workers' compensation claims from 2006-2020 to characterize WRII in firefighters compared to law enforcement officers and "all other" workers. There were 9187 compensable claims for firefighters, 7801 for law enforcement officers, and 586,939 for "all other" workers. Nearly 40% of claims for firefighters were work-related musculoskeletal disorders (WMSDs). The claim rate per 10,000 full-time equivalent (FTE) firefighters was 716.4, which is significantly higher than that of law enforcement officers (510.0) and "all other" workers (163.2). The rate per 10,000 FTE of WMSD claims was also higher in firefighters (277.0) than in law enforcement officers (76.2) and "all other" workers (57.6). Additional WRII among firefighters commonly included being struck or caught in objects, slipping or tripping, and exposure to caustic or noxious substances; and amongst law enforcement, transportation accidents and violence. Medical costs and time-loss days per claim were lower for firefighters and law enforcement than for "all other" workers. Common tasks associated with WMSDs in firefighters included lifting and transporting patients, using specific tools and equipment, and physical training. WMSDs stand out as an area for prevention and intervention activities.


Subject(s)
Firefighters , Occupational Diseases , Occupational Injuries , Humans , Occupational Injuries/epidemiology , Washington/epidemiology , Workers' Compensation , Police , Occupational Diseases/epidemiology
2.
J Occup Environ Med ; 65(2): e51-e56, 2023 02 01.
Article in English | MEDLINE | ID: mdl-36730597

ABSTRACT

OBJECTIVE: Occupational disease presumption laws for firefighters are common, yet little is known about the workers' compensation claims filed for them. METHODS: We identified Washington State's workers' compensation claims for conditions covered by presumption among firefighters during 2000 to 2017, and described them by disease type and claim characteristics. Claim rates for specific cancers were compared with statewide incidence rates using the Washington State cancer registry. RESULTS: Firefighters filed 330 claims for conditions covered by presumption (heart problems, 43%; cancer, 35%; respiratory disease, 15%; and infectious diseases, 8%), with acceptance rates varying by disease and insurer type. Cancer claims were associated with the highest claim costs and time loss. CONCLUSIONS: This study provides necessary baseline data to understand the impact of presumption laws on workers' compensation systems, and can inform future decisions presumption law expansions for firefighters.


Subject(s)
Firefighters , Occupational Diseases , Humans , Workers' Compensation , Washington/epidemiology , Occupational Diseases/epidemiology , Costs and Cost Analysis
3.
PLoS One ; 18(1): e0280307, 2023.
Article in English | MEDLINE | ID: mdl-36649295

ABSTRACT

BACKGROUND: Workers of color experience a disproportionate share of work-related injuries and illnesses (WRII), however, most workers' compensation systems do not collect race and ethnicity information, making it difficult to monitor trends over time, or to investigate specific policies and procedures that maintain or could eliminate the unequal burden of WRII for workers of color. The purpose of this study is to apply a Bayesian method to Washington workers' compensation claims data to identify racial and ethnic disparities of WRII by industry and occupation, improving upon existing surveillance limitations. Measuring differences in risk for WRII will better inform prevention efforts and target prevention to those at increased risk. METHODS: To estimate WRII by race/ethnicity, we applied the Bayesian Improved Surname Geocode (BISG) method to surname and residential address data among all Washington workers' compensation claims filed for injuries in 2013-2017. We then compare worker and injury characteristics by imputed race/ethnicity, and estimate rates of WRII by imputed race/ethnicity within industry and occupation. RESULTS: Black/African Americans had the highest rates of WRII claims across all industry and occupational sectors. Hispanic/Latino WRII claimants also had higher rates than Whites and Asian/Pacific Islanders in almost all industry and occupational sectors. For accepted claims with both medical and non-medical compensation, Bodily reaction/overexertion injuries accounted for almost half of the claims during this reporting period. DISCUSSION: The high rates of injury we report by racial/ethnic categories is a cause for major concern. Nearly all industry and occupation-specific rates of workers' compensation claims are higher for Black/African American and Hispanic/Latino workers compared to Whites. More work is needed to identify work-related, systemic, and individual characteristics.


Subject(s)
Occupational Injuries , Workers' Compensation , Humans , Bayes Theorem , Industry , Occupations , Washington/epidemiology , Occupational Injuries/epidemiology
4.
Workplace Health Saf ; 68(2): 92-102, 2020 Feb.
Article in English | MEDLINE | ID: mdl-31583973

ABSTRACT

Background: The demand for home-based health care support providers (HB providers) is growing as the locus of care shifts to private homes. However, industry representative data of these workers are limited. Methods: Washington Behavioral Risk Factor Surveillance System (WA BRFSS) data from 2011 to 2016 were analyzed to compare HB providers (n = 385) with health care support occupations not based in the home, non-HB providers (n = 229), and all other occupations (n = 32,011). Findings: More HB providers were overweight (4.2%, 95% confidence interval [CI] = [3.3%, 5.2%]) than non-HB providers and all other occupations. Significantly more HB providers had arthritis (33.2%, 95% CI = [27.0%, 39.4%]) and diabetes (9.4%, 95% CI = [5.7%, 13.2%]) than non-HB providers. Nearly twice as many HB providers currently smoked (31.3%, 95% CI = [24.2%, 38.4%]) than non-HB providers. Significantly more HB providers reported serious mental illness (6.8%, 95% CI = [2.8%, 10.7%]) than all other occupations (2.2%). Conclusion/Application to Practice: It is imperative to maintain good health in this home-based health care population as these workers are aging and their professional time becomes limited.


Subject(s)
Allied Health Personnel , Health Status , Home Health Aides , Adult , Behavioral Risk Factor Surveillance System , Chronic Disease/epidemiology , Female , Humans , Male , Mental Disorders/epidemiology , Middle Aged , Occupational Health/statistics & numerical data , Occupational Injuries/epidemiology , Washington/epidemiology
5.
J Occup Environ Med ; 61(9): 747-753, 2019 09.
Article in English | MEDLINE | ID: mdl-31233009

ABSTRACT

OBJECTIVE: Janitorial workers have a high burden of occupational injury and illness, but little information exists on their overall health. METHODS: Data from the Washington State Behavioral Risk Factor Surveillance System (BRFSS) were analyzed to characterize the working population of Janitors in Washington State from 2011 to 2017 (n = 490) as compared with all other workers (n = 38,885). RESULTS: Compared with other workers, Janitors were significantly more diverse, had lower socioeconomic status, and reported poorer general health and higher rates of arthritis and depression. Janitors were less likely to have adequate sleep, health insurance, and access to technology. Janitors reported higher rates of smoking and marijuana use. CONCLUSIONS: Multiple risk factors compound the vulnerability of Janitors. BRFSS data can be used to characterize the health of occupational groups. Identifying overall health needs can better inform policy and help formulate strategies to improve workers' health.


Subject(s)
Behavioral Risk Factor Surveillance System , Household Work , Occupational Health , Occupational Injuries/epidemiology , Occupational Injuries/etiology , Adult , Female , Humans , Male , Middle Aged , Surveys and Questionnaires , Washington/epidemiology , Workers' Compensation
6.
J Agromedicine ; 24(2): 205-214, 2019 04.
Article in English | MEDLINE | ID: mdl-30624159

ABSTRACT

OBJECTIVES: Current industry classification systems in the United States do not differentiate mechanized and nonmechanized logging operations. The objectives of this article are to quantify injury risk differences between mechanized and nonmechanized logging operations in Washington State and to evaluate for potential injury risk tradeoffs, such as decreasing traumatic injuries while increasing nontraumatic injuries that might occur when mechanized logging operations are substituted for nonmechanized logging operations. METHODS: Using Washington State workers' compensation insurance risk classes to differentiate mechanized and nonmechanized logging operations, injury and illness claims data and employer reported hours were used to compare claim rates and to characterize injuries by type of logging operation. RESULTS: From 2005 to 2014, the accepted Washington State worker's compensation claim rate for nonmechanized logging was 46.4 per 100 full-time equivalent employees compared to 6.7 per 100 full-time equivalent (FTE) for mechanized logging activities. The rate ratio for comparing nonmechanized to mechanized logging claims rates for all accepted claims was 6.9 (95% Confidence Interval 6.4-7.5). Claim rates for traumatic injury and nontraumatic injuries in nonmechanized logging exceeded comparable rates in mechanized logging activities, although the distribution of types of injury differed by type of logging operation. A greater percentage of accepted claims in nonmechanized logging were traumatic injuries than in mechanized logging (92.2% vs. 85.0%, respectively). In addition, nonmechanized logging had higher total claim and medical costs per FTE and had a higher proportion of claims with lost work time than mechanized logging. CONCLUSION: Mechanized logging offers a considerable safety advantage over nonmechanized logging operations. Continued efforts to increase the mechanization of logging operations will result in decreased injury rates.


Subject(s)
Accidents, Occupational/economics , Forestry/instrumentation , Occupational Injuries/economics , Workers' Compensation/economics , Accidents, Occupational/statistics & numerical data , Costs and Cost Analysis , Forestry/economics , Humans , Washington
7.
J Interpers Violence ; 34(19): 3995-4019, 2019 10.
Article in English | MEDLINE | ID: mdl-27754922

ABSTRACT

The purpose of this study was to identify characteristics of investigations of sexual abuse concerning vulnerable adults residing in facility settings that were associated with case substantiation. Data on 410 reports of sexual abuse were collected prospectively from Adult Protective Services (APS) and state licensure agency staff in New Hampshire, Oregon, Tennessee, Texas, and Wisconsin over a six-month period. Specifically, we examined differences between reports that were substantiated and those that were not by comparing characteristics of alleged victims, alleged perpetrators, and aspects of investigation using logistic regression. We found that a relatively low proportion of cases (18%) were substantiated overall. Compared to cases that were not substantiated, cases that were substantiated were more likely to feature nursing home residents, older victims, female victims, and allegations of physical contact between the alleged perpetrator and victim. Despite the high proportion of alleged perpetrators who were facility staff (51%) compared to resident perpetrators (25%), cases with resident-to-resident allegations of abuse were much more likely to be substantiated, accounting for 63% of substantiated cases. In light of these findings, we believe it is important that investigators are trained to handle sexual abuse cases appropriately and that they are able to investigate the case thoroughly, promptly, and with as much information as possible. It is also critical that investigators make substantiation decisions using the appropriate standard for confirmation (e.g., preponderance of the evidence, beyond a reasonable doubt, clear and convincing evidence) as state law dictates.


Subject(s)
Crime Victims/psychology , Crime Victims/statistics & numerical data , Residential Facilities , Sex Offenses/psychology , Sex Offenses/statistics & numerical data , Age Distribution , Female , Humans , Logistic Models , Male , Middle Aged , Nursing Homes , Prospective Studies , Sex Distribution , United States
8.
J Occup Environ Med ; 60(12): 1128-1135, 2018 12.
Article in English | MEDLINE | ID: mdl-30252724

ABSTRACT

OBJECTIVE: Assess the effect of chronic comorbidities on hours and earnings recovery following a carpal tunnel syndrome (CTS) claim. METHODS: The hours and earnings profiles of Washington State workers' compensation claimants with CTS and controls, upper extremity fractures (UEF) claimants, were collected by linking to unemployment insurance data during 2007 to 2014. Chronic comorbidity status was determined from workers' compensation bills. RESULTS: More (43%) CTS claimants had diagnosed chronic comorbidities than UEF (24%). CTS claimants and claimants with multiple chronic comorbidities had significantly higher odds of not working post injury and poorer hours and earnings recovery compared with UEF claimants and those with no chronic comorbidities. CONCLUSIONS: This research suggests that chronic conditions should be considered as barriers to return to work among injured workers.


Subject(s)
Carpal Tunnel Syndrome/epidemiology , Chronic Disease/epidemiology , Fractures, Bone/epidemiology , Occupational Diseases/epidemiology , Workers' Compensation/economics , Adult , Carpal Tunnel Syndrome/economics , Chronic Disease/economics , Comorbidity , Female , Humans , Humeral Fractures/epidemiology , Male , Middle Aged , Occupational Diseases/economics , Radius Fractures/epidemiology , Retrospective Studies , Time Factors , Ulna Fractures/epidemiology , Washington/epidemiology
9.
J Safety Res ; 65: 53-58, 2018 06.
Article in English | MEDLINE | ID: mdl-29776529

ABSTRACT

INTRODUCTION: Construction is high-hazard industry, and continually ranks among those with the highest workers' compensation (WC) claim rates in Washington State (WA). However, not all construction firms are at equal risk. We tested the ability to identify those construction firms most at risk for future claims using only administrative WC and unemployment insurance data. METHODS: We collected information on construction firms with 10-50 average full time equivalent (FTE) employees from the WA unemployment insurance and WC data systems (n=1228). Negative binomial regression was used to test the ability of firm characteristics measured during 2011-2013 to predict time-loss claim rates in the following year, 2014. RESULTS: Claim rates in 2014 varied by construction industry groups, ranging from 0.7 (Land Subdivision) to 4.6 (Foundation, Structure, and Building Construction) claims per 100 FTE. Construction firms with higher average WC premium rates, a history of WC claims, increasing number of quarterly FTE, and lower average wage rates during 2011-2013 were predicted to have higher WC claim rates in 2014. CONCLUSIONS: We demonstrate the ability to leverage administrative data to identify construction firms predicted to have future WC claims. This study should be repeated to determine if these results are applicable to other high-hazard industries. Practical Applications: This study identified characteristics that may be used to further refine targeted outreach and prevention to construction firms at risk.


Subject(s)
Construction Industry/statistics & numerical data , Workers' Compensation/statistics & numerical data , Adult , Data Systems , Humans , Middle Aged , Risk , Washington , Young Adult
11.
Am J Ind Med ; 60(5): 457-471, 2017 May.
Article in English | MEDLINE | ID: mdl-28295479

ABSTRACT

BACKGROUND: Work-related musculoskeletal disorders (WMSDs) are common and place large economic and social burdens on workers and their communities. We describe recent WMSD trends and patterns of WMSD incidence among the Washington worker population by industry. METHODS: We used Washington State's workers' compensation compensable claims from 1999 to 2013 to describe incidence and cost of WMSD claims by body part and diagnosis, and to identify high-risk industries. RESULTS: WMSD claim rates declined by an estimated annual 5.4% (95% CI: 5.0-5.9%) in Washington State from 1999 to 2013, but WMSDs continue to account for over 40% of all compensable claims. High risk industries identified were Construction; Transportation and Warehousing; Health Care and Social Assistance; and Manufacturing. CONCLUSIONS: As documented in other North American contexts, this study describes an important decline in the incidence of WMSDs. The Washington State workers' compensation system provides a rich data source for the surveillance of WMSDs.


Subject(s)
Musculoskeletal Diseases/epidemiology , Musculoskeletal Diseases/etiology , Occupational Diseases/epidemiology , Adolescent , Adult , Aged , Databases, Factual , Female , Humans , Industry/statistics & numerical data , Insurance Claim Reporting , Male , Middle Aged , Musculoskeletal Diseases/economics , Occupational Diseases/economics , Occupational Injuries/economics , Occupational Injuries/epidemiology , Sex Distribution , Washington/epidemiology , Workers' Compensation , Young Adult
12.
J Occup Environ Med ; 58(8): 760-4, 2016 08.
Article in English | MEDLINE | ID: mdl-27414007

ABSTRACT

OBJECTIVE: The aim of this study was to identify relationships between work organizational variables (job rotation, overtime work, having a second job, and work pacing) (These work organizational variables and their relationships with biomechanical and psychosocial exposures were studied previously and published in a separate paper.) and health outcome measures [carpal tunnel syndrome (CTS), lateral and medial epicondylitis (LEPI/MEPI)]. METHODS: Using a pooled baseline cohort of 1834 subjects, the relationships were studied using logistic regression models. RESULTS: Varied degrees of associations between the work organizational and outcomes variables were found. Job rotation was significantly associated with being a CTS case [odds ratio (OR) = 1.23, 95% confidence interval (95% CI): 1.00 to 1.50]. Overtime work was significantly associated with lower LEPI prevalence (OR = 0.48, 95% CI: 0.28 to 0.84). No statistically significant associations were found between having a second job and different work pacing and any of the three health outcome measures. CONCLUSIONS: Work organizational variables were only partially associated with the studied health outcomes.


Subject(s)
Carpal Tunnel Syndrome/epidemiology , Occupational Diseases/epidemiology , Tennis Elbow/epidemiology , Workload , Adult , Female , Humans , Male , Middle Aged , Prevalence , Risk Factors , Workplace/organization & administration
13.
Disaster Med Public Health Prep ; 10(3): 378-85, 2016 06.
Article in English | MEDLINE | ID: mdl-27074115

ABSTRACT

OBJECTIVE: This project aimed to describe demographic patterns and circumstances surrounding injury deaths in New York City (NYC) related to Hurricane Sandy. METHODS: Injury deaths related to Hurricane Sandy were classified by using data from multiple sources: NYC's Office of Vital Statistics death records, Office of Chief Medical Examiner case investigation files, and American Red Cross disaster mortality data. Injury deaths were classified as being related to Hurricane Sandy if they were caused directly by the storm's environmental forces or if they were indirectly caused by an interruption of services, displacement, or other lifestyle disruption. RESULTS: We identified 52 injury deaths in NYC related to Hurricane Sandy. Most decedents were male (75%); nearly half were aged 65 years and older (48%). Most (77%) deaths were caused by injuries directly related to Hurricane Sandy. Ninety percent of direct deaths were caused by drowning; most (73%) occurred within 3 days of landfall. Half (50%) of the 12 indirect deaths that occurred up to 30 days after the storm were caused by a fall. Nearly two-thirds (63%) were injured at home. Three-quarters (75%) of fatal injuries occurred in evacuation Zone A. CONCLUSIONS: Risk communication should focus on older adults, males, and those living in evacuation zones; more evacuation assistance is necessary. NYC's fatal injury profile can inform future coastal storm planning efforts. (Disaster Med Public Health Preparedness. 2016;10:378-385).


Subject(s)
Cyclonic Storms/mortality , Wounds and Injuries/epidemiology , Adolescent , Adult , Aged , Child , Child, Preschool , Death Certificates , Female , Humans , Male , Middle Aged , New York City/epidemiology
14.
Inj Epidemiol ; 2(1): 11, 2015 Dec.
Article in English | MEDLINE | ID: mdl-27747743

ABSTRACT

BACKGROUND: The New York City emergency department (ED) syndromic surveillance (SS) system provides near real-time data on the majority of ED visits. The utility of ED SS for injury surveillance has not been thoroughly evaluated. We created injury syndromes based on ED chief complaint information and evaluated their utility compared to administrative billing data. METHODS: Six injury syndromes were developed: traffic-related injuries to pedal cyclists, pedestrians, and motor vehicle occupants; fall-related injuries; firearm-related injuries; and assault-related stabbings. Daily injury counts were compared for ED SS and the administrative billing data for years 2008-2010. We examined characteristics of injury trends and patterns between the two systems, calculating descriptive statistics for temporal patterns and Pearson correlation coefficients (r) for temporal trends. We also calculated proportions of demographic and geospatial patterns for both systems. RESULTS: Although daily volume of the injuries varied between the two systems, the temporal patterns were similar (all r values for daily volume exceeded 0.65). Comparisons of injuries by time of day, day of week, and quarter of year demonstrated high agreement between the two systems-the majority had an absolute percentage point difference of 2.0 or less. Distributions of injury by sex and age group also aligned well. Distribution of injury by neighborhood of residence showed mixed results-some neighborhood comparisons showed a high level of agreement between systems, while others were less successful. CONCLUSIONS: As evidenced by the strong positive correlation coefficients and the small absolute percentage point differences in our comparisons, we conclude that ED SS captures temporal trends and patterns of injury-related ED visits effectively. The system could be used to identify changes in injury patterns, allowing for situational awareness during emergencies, timely response, and public messaging.

15.
J Agromedicine ; 16(3): 163-73, 2011.
Article in English | MEDLINE | ID: mdl-21728868

ABSTRACT

The average age of United States farmers has been increasing for 20 years. The objective is to examine the factors associated with hours worked among farmers age 50 and older. A cohort of Kentucky and South Carolina farmers (n = 1394) over age 50 were surveyed annually during 2002-2005. Of those that reported any farm work, males worked 24 mean hours/week and females worked 14 mean hours/week. Greater satisfaction and more experience farming, increased acreage, and presence of animals significantly increased estimated hours farmed, whereas chronic health problems, although prevalent, had a minor role in determining work hours.


Subject(s)
Agriculture/statistics & numerical data , Workload/statistics & numerical data , Age Distribution , Aged , Aged, 80 and over , Cohort Studies , Female , Health Status , Humans , Kentucky , Male , Middle Aged , Personal Satisfaction , Regression Analysis , Sex Distribution , South Carolina , Workload/psychology
16.
Curr Aging Sci ; 4(2): 158-70, 2011 Jul.
Article in English | MEDLINE | ID: mdl-21235492

ABSTRACT

The current analysis reexamines the relationship between supplemental vitamin E and all-cause mortality. All randomized, controlled trials testing the treatment effect of vitamin E supplementation in adults for at least one year were sought. MEDLINE, the Cochrane Library, and Biological Abstracts databases were searched using the terms "vitamin E," "alpha-tocopherol," "antioxidants," "clinical trial," and "controlled trial" for studies published through April 2010; results were limited to English, German, or Spanish language articles. Studies were also obtained through reference mining. All randomized controlled trials using vitamin E, with a supplementation period of at least one year, to prevent or treat disease in adults were identified and abstracted independently by two raters. Mortality data from trials with a supplementation period of at least one year were pooled. The selected trials (n = 57) were published between 1988 and 2009. Sample sizes range from 28 to 39,876 (median = 423), yielding 246,371 subjects and 29,295 all-cause deaths. Duration of supplementation for the 57 trials range from one to 10.1 years (median = 2.6 years). A random effects meta-analysis produce an overall risk ratio of 1.00 (95% confidence interval: 0.98, 1.02); additional analyses suggest no relationship between dose and risk of mortality. Based on the present meta-analysis, supplementation with vitamin E appears to have no effect on all-cause mortality at doses up to 5,500 IU/d.


Subject(s)
Antioxidants/therapeutic use , Dietary Supplements , Vitamin E/therapeutic use , Antioxidants/adverse effects , Cause of Death , Dietary Supplements/adverse effects , Humans , Longevity , Risk Assessment , Risk Factors , Time Factors , Treatment Outcome , Vitamin E/adverse effects
17.
J Elder Abuse Negl ; 20(4): 353-76, 2008.
Article in English | MEDLINE | ID: mdl-19042662

ABSTRACT

This article reports research findings concerning 119 alleged sexual perpetrators who were reported to state authorities for abusing elderly individuals residing in care facilities. The largest group of accused was employees of the facilities, followed by facility residents. Family members of the alleged victims and visitors to the facilities also were among those reported as sexually abusive. Investigation of the allegations by Adult Protective Services and regulatory staff resulted in 32 of these individuals being confirmed as sexual perpetrators against vulnerable elders. Male and female alleged and confirmed sexual perpetrators were identified as well as both male and female elderly sexual abuse victims. Perpetrator characteristics, victim vulnerabilities, abuse acts, locations of assaults, and available case outcomes are presented. Implications of the findings are discussed.


Subject(s)
Coercion , Crime Victims/statistics & numerical data , Elder Abuse/statistics & numerical data , Sex Offenses/statistics & numerical data , Vulnerable Populations/statistics & numerical data , Adult , Aged , Aged, 80 and over , Elder Abuse/psychology , Female , Homes for the Aged , Humans , Interpersonal Relations , Male , Middle Aged , Nursing Homes , Sex Offenses/psychology , Sexual Partners , Socioeconomic Factors , United States/epidemiology , Vulnerable Populations/psychology
18.
Biochem Biophys Res Commun ; 334(2): 342-8, 2005 Aug 26.
Article in English | MEDLINE | ID: mdl-16018967

ABSTRACT

The hippocampus of Alzheimer's disease brain has been shown to be highly oxidized compared to age-matched controls. One of the most sensitive targets of oxidation is anionic sulfur which can be found within the active site of members of the cysteine-protease family. Thus, while members of the cysteine-protease family such as the calpains and caspases have been found to be in an active conformation in vulnerable brain regions in AD it is possible that their proteolytic activity is hampered due to the robust oxidative stress found at these locations. To address this issue, the amount of caseinolytic activity present in the hippocampus from post-mortem brain samples of AD and age-matched controls was determined. No difference in caseinolytic activity in the absence of exogenous reducing agent was observed between AD and control. However, after addition of the thiol-specific reducing agent, dithiothreitol (DTT), the amount of caseinolytic activity was significantly increased in AD compared to the DTT-mediated increase in control. This suggests that the cysteine proteases are more oxidized in AD brain and that latent proteolytic activity in AD brain can be released by antioxidants. Further testing revealed that the calcium-dependent caseinolytic activity was significantly lower in AD brain compared to controls. This is despite the fact that the major calcium-dependent thiol protease, calpain, is threefold more activated in AD brain based on autolytic activation measured by Western blotting. This calcium-dependent protease difference between AD and control brains was negated by addition of DTT. These data suggest that cysteine protease activity in AD brain is inactivated by oxidants, which is evident by the ability of thiol-specific reducing agents such as DTT to rescue and recover activity.


Subject(s)
Alzheimer Disease/enzymology , Cysteine Endopeptidases/chemistry , Hippocampus/enzymology , Sulfhydryl Compounds/chemistry , Aged , Aged, 80 and over , Calcium/chemistry , Caseins/chemistry , Cysteine Endopeptidases/analysis , Enzyme Activation , Female , Humans , In Vitro Techniques , Male , Oxidation-Reduction , Oxidative Stress , Reference Values
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