Raw and Pre-Treated Styrene Butadiene Rubber (SBR) Dust as a Partial Replacement for Natural Sand in Mortars.

The circular economy (CE) is widely known for its emphasis on reducing waste and maximizing the use of resources by reusing, recycling, and repurposing materials to create a sustainable and efficient system. The CE is based on 3R-reuse, reduce, and recycle. The aim of this article is to use styrene butadiene rubber dust (SBR) in building material, constituting secondary waste in the production of SBR, which is currently disposed of as landfill. SBR is partly intended to replace the natural raw material sand. The purpose of the final material is to use it for its light weight, insulating properties, or ability to absorb vibrations and sounds. Various shares of SBR dust in mortars were tested. Some of the mortars used SBR thermal pre-treatment at temperatures of 200, 275, and 350 °C. The strength and SEM results are presented. The best pre-treatment for SBR dust is thermal treatment at 275 °C. The maximum usage of rubber dust with thermal treatment is 60% as a sand substitute. The novel finding of this study is the possibility to use more than 30% rubber dust (as a substitute for sand) thanks to pre-treatment, whereby 30% is a common maximum ratio in mortars.

In Vitro Evaluation of Poly(lactide-co-glycolide) In Situ Forming Gels for Bedaquiline Fumarate Salt and Pharmacokinetics Following Subcutaneous Injection in Rats.

This study evaluated in vitro and in vivo drug release of bedaquiline from in situ forming gels (ISGs) containing 200 mg eq./g bedaquiline fumarate salt prepared with four different grades of poly(d,l-lactide) (PDLLA) or poly(d,l-lactide-co-glycolide) (PLGA) with a lactide/glycolide ratio of 50/50 or 75/25 and acid (A) or ester (E) end-capping in N-methyl-2-pyrrolidone at a polymer/solvent ratio of 20/80% (w/w). Mean in vitro drug release in 0.05 M phosphate buffer pH 7.4 with 1% (w/v) sodium lauryl sulphate was 37.3, 47.1, 53.3, and 62.3% within 28 days for ISGs containing PLGA5050A, PDLLA, PLGA7525A, and PLGA7525E, respectively. The data suggested that drug release was primarily controlled by precipitated drug redissolving, rather than polymer erosion. In vivo pharmacokinetic profiles after subcutaneous injections in rats were comparable for all ISGs (mean half-lives (t1/2) ranged from 1411 to 1695 h) and indicated a sustained drug release when compared to a solution of bedaquiline fumarate salt in polyethylene glycol 400/water 50/50% (v/v) (mean t1/2 of 895 h). In conclusion, PLGA or PDLLA-based ISGs have shown potential for parenteral sustained delivery of bedaquiline, suggesting further preclinical and clinical studies. From a formulation point of view, this case example highlights the importance of the interplay between drug solubility in biological media and dissolution of drug precipitates, which, in addition to the incorporation of diffusion controlling polymers, governs the release of the active drug.

A label-free potentiometric sensor principle for the detection of antibody-antigen interactions.

We report here on a new potentiometric biosensing principle for the detection of antibody-antigen interactions at the sensing membrane surface without the need to add a label or a reporter ion to the sample solution. This is accomplished by establishing a steady-state outward flux of a marker ion from the membrane into the contacting solution. The immunobinding event at the sensing surface retards the marker ion, which results in its accumulation at the membrane surface and hence in a potential response. The ion-selective membranes were surface-modified with an antibody against respiratory syncytial virus using click chemistry between biotin molecules functionalized with a triple bond and an azide group on the modified poly (vinyl chloride) group of the membrane. The bioassay sensor was then built up with streptavidin and subsequent biotinylated antibody. A quaternary ammonium ion served as the marker ion. The observed potential was found to be modulated by the presence of respiratory syncytial virus bound on the membrane surface. The sensing architecture was confirmed with quartz crystal microbalance studies, and stir effects confirmed the kinetic nature of the marker release from the membrane. The sensitivity of the model sensor was compared to that of a commercially available point-of-care test, with promising results.

A preliminary study for identifying olfactory markers of fear in the rat.

In stressful situations, many animals release alarm pheromones to warn conspecifics of impending danger. The authors sought to establish experimental conditions for a larger study aimed at identifying alarm pheromones emitted by the rat. They placed rats in a specially designed chamber and exposed them to aversive tactile, visual and acoustic stimuli over the course of a few days. The researchers observed rats' behavior and analyzed air samples taken from their immediate environment under the following conditions: (i) when rats were unstressed; (ii) immediately after rats were exposed to aversive stimuli; and (iii) when rats were left alone in the chamber after being conditioned to fear imminent aversive stimuli. Stressed rats emitted several substances that are known to function as alarm pheromones in insects. When previously unstressed control rats were exposed to these same substances, they had a distinct behavioral fear response.

["Alarm substances" as biological markers indicating emotional state before death]. / Substancje alarmowe jako wykladnik stanu emocjonalnego poprzedzajacego zgon.

This scientific project presents the results of preliminary examinations aimed to identify alarm pheromones secreted by mammals. Wistar male rats were used for the experiment. Animals were treated by aversive sensoric stimuli and fear conditioning procedures. The animals' behaviour was registered. Quantitative and qualitative analysis of air samples taken from their environment was conducted with the use of GC-FID and GC-MSD technics. In the rhinocephalon structures (olfactory bulb, olfactory tract) the concentration of glutamate was measured. During the progress of the experiment increasing behavioral reactions of anxiety were observed in the rats. In their atmosphere organic compounds were identified. Some of them can be considered to be alarm substances. In the examined structures of the rhinocephalon on increased concentration of glutamate in each individual was revealed.