ABSTRACT
Inflammatory mediators constitute a recently coined term in the field of metal-based complexes with antiplatelet activities. Our strategy targets Platelet-Activating Factor (PAF) and its receptor, which is the most potent lipid mediator of inflammation. Thus, the antiplatelet (anti-PAF) potency of any substance could be exerted by inhibiting the PAF-induced aggregation in washed rabbit platelets (WRPs), which internationally is a well-accepted methodology. Herein, a series of mononuclear (mer-[Cr(pqx)Cl3(H2O]) (1), [Co(pqx)Cl2(DMF)] (2) (DMF = N,N'-dimethyl formamide), [Cu(pqx)Cl2(DMSO)] (3) (DMSO = dimethyl sulfoxide), [Zn(pqx)Cl2] (4)) and dinuclear complexes ([Mn(pqx)(H2O)2Cl2]2 (5), [Fe(pqx)Cl2]2 (6) and [Ni(pqx)Cl2]2 (7)) incorporating the 2-(2'-pyridyl)quinoxaline ligand (pqx), were biologically evaluated as inhibitors of the PAF- and thrombin-induced aggregation in washed rabbit platelets (WRPs). The molecular structure of the five-co-ordinate analog (3) has been elucidated by single-crystal X-ray diffraction revealing a trigonal bipyramidal geometry. All complexes are potent inhibitors of the PAF-induced aggregation in WRPs in the micromolar range. Complex (6) displayed a remarkable in vitro dual inhibition against PAF and thrombin, with IC50 values of 1.79 µM and 0.46 µM, respectively. Within the series, complex (5) was less effective (IC50 = 39 µM) while complex (1) was almost 12-fold more potent against PAF, as opposed to thrombin-induced aggregation. The biological behavior of complexes 1, 6 and 7 on PAF's basic metabolic enzymatic pathways reveals that they affect key biosynthetic and catabolic enzymes of PAF underlying the anti-inflammatory properties of the relevant complexes. The in vitro cytotoxic activities of all complexes in HEK293T (human embryonic kidney cells) and HeLa cells (cervical cancer cells) are described via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. The results reveal that complex 3 is the most potent within the series.
Subject(s)
Antineoplastic Agents , Coordination Complexes , Transition Elements , Animals , Humans , Rabbits , Platelet Aggregation , Platelet Activating Factor/pharmacology , Platelet Activating Factor/metabolism , Blood Platelets/metabolism , Thrombin/metabolism , Coordination Complexes/pharmacology , Coordination Complexes/metabolism , Ligands , Inflammation Mediators/metabolism , Dimethyl Sulfoxide/pharmacology , Quinoxalines/pharmacology , HEK293 Cells , HeLa Cells , Antineoplastic Agents/pharmacology , Transition Elements/metabolismABSTRACT
In this work, a voltammetric method based on a metal organic framework (Ca-MOF)-modified carbon paste electrode for lead determination was developed. The MOF-based electrode was packed in a new type of 3D-printed syringe-type integrated device, which was entirely fabricated by a dual extruder 3D printer. After optimization of the operational parameters, a limit of detection of 0.26 µg L-1 Pb(II) was achieved, which is lower than that of existing MOF-based lead sensors. The device was used for Pb(II) determination in fish feed and bottled water samples with high accuracy and reliability. The proposed sensor is suitable for on-site analyses and provides a low-cost integrated transducer for the ultrasensitive routine detection of lead in practical applications.
