ABSTRACT
This study was carried out to investigate fifteen cases of acute lethal infection of calves (Subject(s)
Cattle Diseases/parasitology
, Disease Outbreaks/veterinary
, Lymphocytes/pathology
, Skin Diseases/veterinary
, Theileria annulata/isolation & purification
, Theileriasis/pathology
, Animals
, Base Sequence
, Cattle
, Cattle Diseases/epidemiology
, Cattle Diseases/pathology
, Cell Growth Processes/physiology
, DNA, Protozoan/chemistry
, DNA, Protozoan/genetics
, Female
, Immunohistochemistry/veterinary
, Lymphocytes/parasitology
, Male
, Molecular Sequence Data
, Polymerase Chain Reaction/veterinary
, Portugal/epidemiology
, RNA, Ribosomal, 18S/chemistry
, RNA, Ribosomal, 18S/genetics
, Sequence Analysis, DNA
, Skin Diseases/epidemiology
, Skin Diseases/parasitology
, Skin Diseases/pathology
, Theileriasis/epidemiology
, Theileriasis/parasitology
ABSTRACT
An outbreak of severe respiratory disease in a goat herd was associated with Mycoplasma ovipneumoniae, Mycoplasma arginini, Mannheimia haemolytica and Pasteurella multocida with mortality rates exceeding 20% in kids. Post mortem features in affected kids included severe pleuropneumonia, lung consolidation, large quantities of pleural fluid and pericarditis. This is the first report of atypical proliferative pneumonia in goats in Portugal.
Subject(s)
Goat Diseases/epidemiology , Mannheimia haemolytica/isolation & purification , Mycoplasma ovipneumoniae/isolation & purification , Pasteurellosis, Pneumonic/epidemiology , Pneumonia, Mycoplasma/veterinary , Animals , Animals, Newborn , Disease Outbreaks/veterinary , Female , Goat Diseases/microbiology , Goats , Male , Pneumonia, Mycoplasma/epidemiology , Portugal/epidemiologyABSTRACT
A modified Rose Bengal test (mRB) and an indirect ELISA (iELISA) with Protein G as the conjugate, were evaluated for the diagnosis of Brucella melitensis infection in unvaccinated sheep with a known bacteriological status, and their diagnostic efficacy was compared with that of the standard Rose Bengal (RB) and Complement Fixation (CF) tests used in the current eradication campaign in EU countries. All tests showed 100% specificity when testing the sera from 212 Brucella-free sheep. When testing the sera from 219 Brucella melitensis culture-positive sheep, both the mRB and iELISA tests were more sensitive (98.6% and 96.8%, respectively) than the RB and CF tests (95.0% and 92.7%, respectively). These results were similar when testing the sera from 181 animals belonging to infected flocks but found bacteriologically negative, suggesting that the mRB or iELISA tests could advantageously replace the current RB procedure used as the screening test.
