ABSTRACT
A fast method for processing biologic material for electron microscopy for precise and specific diagnosis of infectious agents is an increasing necessity. After different, reportedly fast methods were tested, a useful and quick technique was developed that provides well-preserved cellular structures, enabling the etiologic diagnosis of infectious agents even in necrotic tissue or other biologic material such as sputum, bronchoalveolar lavage, and the like. This procedure takes less than 3 hours.
Subject(s)
Histocytological Preparation Techniques , Infections/diagnosis , Microscopy, Electron , Acquired Immunodeficiency Syndrome/complications , Bronchoalveolar Lavage Fluid/microbiology , Bronchoalveolar Lavage Fluid/parasitology , Humans , Intestine, Large/microbiology , Intestine, Large/parasitology , Intestine, Large/ultrastructure , Liver/microbiology , Liver/parasitology , Liver/ultrastructure , Lung/microbiology , Lung/parasitology , Lung/ultrastructure , Lymph Nodes/microbiology , Lymph Nodes/parasitology , Lymph Nodes/ultrastructure , Pneumonia, Pneumocystis/complications , Pneumonia, Pneumocystis/diagnosis , Sputum/microbiology , Sputum/parasitology , Toxoplasmosis/complications , Toxoplasmosis/diagnosisABSTRACT
Normal human liver fragments obtained through intra surgical needle biopsies from five selected hospital cases were fixed in glutaraldehyde and subsequently in osmium tetroxide and embedded in Araldite. These samples were studied in semithin sections under light microscopy. Stereological methods applied on photomicrographs were used to estimate the hepatocyte nuclear volume, the nuclear and cytoplasmic volume densities as well as the hepatocyte numerical density in the liver intermediate lobular zone. The average absolute volume of the mean hepatocyte in the intermediate lobular zone is 2850 +/- 99.9 microns 3. In one cubic centimeter of this zone there are 233 x 10(6) +/- 81.57 parenchymal cells.
Subject(s)
Liver/cytology , Biopsy, Needle , Humans , Microscopy, Electron , Reference ValuesABSTRACT
Liver parenchymal cell parasitism with the amastigotes form of L. donovani was detected by electron microscopy in human visceral leishmaniasis. Endocytosis was considered to be the mechanism by which the leishmania entered the cell. Evidence of well preserved parasites within hepatocytes suggest this parasitism as a possible reservoir for recrudescence.
Subject(s)
Leishmaniasis, Visceral/parasitology , Liver/parasitology , Humans , Leishmaniasis, Visceral/pathology , Liver/pathology , Liver/ultrastructure , Microscopy, ElectronSubject(s)
Antigen-Antibody Complex/immunology , Chagas Disease/immunology , Immune Complex Diseases , Animals , Arterioles/immunology , Capillaries/immunology , Complement C3/analysis , Fluorescent Antibody Technique , Immunoglobulin G/analysis , Male , Mice , Myocardium/pathology , Trypanosoma cruzi/immunologyABSTRACT
Complexos imunes circulantes (IC), apresentando capacidade reativa para C1q homologo e humano, foram observados em soros de quatro caes infectados com Leishmania donovani, a partir da 3a. semana, persistindo durante doze meses de evolucao da doenca. Os complexos imunes, previamente concentrados com Polietilenoglicol 6.000, sao constituidos de antigenos de L.donovani, anticorpos das classes IgM e IgG e fragmentos do componente C3 do Complemento. Durante o periodo observado, formaram-se pelo menos quatro tipos de IC; dois, nos quais os anticorpos sao de classe IgM,porem ligados a antigenos diferentes entre si: antigenos do parasita ou IgG; os outros dois sao formados por anticorpos de classe IgG, tambem ligados a antigenos diferentes: parasitarios ou a propria IgG