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1.
Mol Cell Biol ; 43(5): 185-199, 2023.
Article in English | MEDLINE | ID: mdl-37140180

ABSTRACT

Rif1 mediates telomere length, DNA replication, and DNA damage responses in budding yeast. Previous work identified several posttranslational modifications of Rif1, however none of these was shown to mediate the molecular or cellular responses to DNA damage, including telomere damage. We searched for such modifications using immunoblotting methods and the cdc13-1 and tlc1Δ models of telomere damage. We found that Rif1 is phosphorylated during telomere damage, and that serines 57 and 110 within a novel phospho-gate domain (PGD) of Rif1 are important for this modification, in cdc13-1 cells. The phosphorylation of Rif1 appeared to inhibit its accumulation on damaged chromosomes and the proliferation of cells with telomere damage. Moreover, we found that checkpoint kinases were upstream of this Rif1 phosphorylation and that the Cdk1 activity was essential for maintaining it. Apart from telomere damage, S57 and S110 were essential for Rif1 phosphorylation during the treatment of cells with genotoxic agents or during mitotic stress. We propose a speculative "Pliers" model to explain the role of the PGD phosphorylation during telomere and other types of damage.


Subject(s)
Repressor Proteins , Saccharomyces cerevisiae Proteins , Telomere-Binding Proteins , Telomere , DNA Replication , Phosphorylation , Repressor Proteins/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Telomere/metabolism , Telomere-Binding Proteins/genetics , Telomere-Binding Proteins/metabolism
2.
Genetics ; 203(4): 1659-68, 2016 08.
Article in English | MEDLINE | ID: mdl-27334270

ABSTRACT

Inverted chromosome duplications or palindromes are linked with genetic disorders and malignant transformation. They are considered by-products of DNA double-strand break (DSB) repair: the homologous recombination (HR) and the nonhomologous end joining (NHEJ). Palindromes near chromosome ends are often triggered by telomere losses. An important question is to what extent their formation depends upon DSB repair mechanisms. Here we addressed this question using yeast genetics and comparative genomic hybridization. We induced palindrome formation by passaging cells lacking any form of telomere maintenance (telomerase and telomere recombination). Surprisingly, we found that DNA ligase 4, essential for NHEJ, did not make a significant contribution to palindrome formation induced by telomere losses. Moreover RAD51, important for certain HR-derived mechanisms, had little effect. Furthermore RAD52, which is essential for HR in yeast, appeared to decrease the number of palindromes in cells proliferating without telomeres. This study also uncovered an important role for Rev3 and Rev7 (but not for Pol32) subunits of polymerase ζ in the survival of cells undergoing telomere losses and forming palindromes. We propose a model called short-inverted repeat-induced synthesis in which DNA synthesis, rather than DSB repair, drives the inverted duplication triggered by telomere dysfunction.


Subject(s)
DNA Ligase ATP/genetics , DNA-Directed DNA Polymerase/genetics , Inverted Repeat Sequences/genetics , Saccharomyces cerevisiae Proteins/genetics , Telomerase/genetics , DNA End-Joining Repair/genetics , Homologous Recombination/genetics , Rad51 Recombinase/genetics , Rad52 DNA Repair and Recombination Protein/genetics , Recombinational DNA Repair/genetics , Saccharomyces cerevisiae/genetics , Telomere , Telomere Homeostasis
3.
Aging Cell ; 15(3): 553-62, 2016 06.
Article in English | MEDLINE | ID: mdl-27004475

ABSTRACT

Telomere attrition is linked to cancer, diabetes, cardiovascular disease and aging. This is because telomere losses trigger further genomic modifications, culminating with loss of cell function and malignant transformation. However, factors regulating the transition from cells with short telomeres, to cells with profoundly altered genomes, are little understood. Here, we use budding yeast engineered to lack telomerase and other forms of telomere maintenance, to screen for such factors. We show that initially, different DNA damage checkpoint proteins act together with Exo1 and Mre11 nucleases, to inhibit proliferation of cells undergoing telomere attrition. However, this situation changes when survivors lacking telomeres emerge. Intriguingly, checkpoint pathways become tolerant to loss of telomeres in survivors, yet still alert to new DNA damage. We show that Rif1 is responsible for the checkpoint tolerance and proliferation of these survivors, and that is also important for proliferation of cells with a broken chromosome. In contrast, Exo1 drives extensive genomic modifications in survivors. Thus, the conserved proteins Rif1 and Exo1 are critical for survival and evolution of cells with lost telomeres.


Subject(s)
Exodeoxyribonucleases/metabolism , Genomic Instability , Repressor Proteins/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/genetics , Telomere-Binding Proteins/metabolism , Telomere/metabolism , Cell Cycle Checkpoints/genetics , Cell Proliferation/genetics , Cellular Senescence/genetics , Chromosomes, Fungal/metabolism , DNA Breaks, Double-Stranded , Endonucleases/metabolism , Gene Deletion , Microbial Viability/genetics , Models, Biological , Phenotype , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/growth & development
4.
Nucleic Acids Res ; 44(8): 3728-38, 2016 05 05.
Article in English | MEDLINE | ID: mdl-26883631

ABSTRACT

Damaged DNA can be repaired by removal and re-synthesis of up to 30 nucleotides during base or nucleotide excision repair. An important question is what happens when many more nucleotides are removed, resulting in long single-stranded DNA (ssDNA) lesions. Such lesions appear on chromosomes during telomere damage, double strand break repair or after the UV damage of stationary phase cells. Here, we show that long single-stranded lesions, formed at dysfunctional telomeres in budding yeast, are re-synthesized when cells are removed from the telomere-damaging environment. This process requires Pol32, an accessory factor of Polymerase δ. However, re-synthesis takes place even when the telomere-damaging conditions persist, in which case the accessory factors of both polymerases δ and ε are required, and surprisingly, salt. Salt added to the medium facilitates the DNA synthesis, independently of the osmotic stress responses. These results provide unexpected insights into the DNA metabolism and challenge the current view on cellular responses to telomere dysfunction.


Subject(s)
DNA Polymerase III/metabolism , DNA Polymerase II/metabolism , DNA Repair , Sodium Chloride/pharmacology , Telomere/enzymology , Cell Proliferation/drug effects , Chromosomes, Fungal/drug effects , Chromosomes, Fungal/enzymology , Chromosomes, Fungal/metabolism , DNA Polymerase I/physiology , DNA, Fungal/biosynthesis , DNA-Binding Proteins/metabolism , DNA-Directed DNA Polymerase/metabolism , DNA-Directed DNA Polymerase/physiology , Mitogen-Activated Protein Kinases/metabolism , Phleomycins/pharmacology , Saccharomyces cerevisiae/drug effects , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae Proteins/physiology , Telomere/drug effects , Telomere/metabolism , Telomere Homeostasis , Transcription Factors/metabolism
5.
PLoS Genet ; 7(12): e1002417, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22194703

ABSTRACT

Cells accumulate single-stranded DNA (ssDNA) when telomere capping, DNA replication, or DNA repair is impeded. This accumulation leads to cell cycle arrest through activating the DNA-damage checkpoints involved in cancer protection. Hence, ssDNA accumulation could be an anti-cancer mechanism. However, ssDNA has to accumulate above a certain threshold to activate checkpoints. What determines this checkpoint-activation threshold is an important, yet unanswered question. Here we identify Rif1 (Rap1-Interacting Factor 1) as a threshold-setter. Following telomere uncapping, we show that budding yeast Rif1 has unprecedented effects for a protein, inhibiting the recruitment of checkpoint proteins and RPA (Replication Protein A) to damaged chromosome regions, without significantly affecting the accumulation of ssDNA at those regions. Using chromatin immuno-precipitation, we provide evidence that Rif1 acts as a molecular "band-aid" for ssDNA lesions, associating with DNA damage independently of Rap1. In consequence, small or incipient lesions are protected from RPA and checkpoint proteins. When longer stretches of ssDNA are generated, they extend beyond the junction-proximal Rif1-protected regions. In consequence, the damage is detected and checkpoint signals are fired, resulting in cell cycle arrest. However, increased Rif1 expression raises the checkpoint-activation threshold to the point it simulates a checkpoint knockout and can also terminate a checkpoint arrest, despite persistent telomere deficiency. Our work has important implications for understanding the checkpoint and RPA-dependent DNA-damage responses in eukaryotic cells.


Subject(s)
Replication Protein A/metabolism , Repressor Proteins/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Telomere-Binding Proteins/metabolism , Cell Cycle Checkpoints/genetics , Cell Cycle Proteins/genetics , Cell Cycle Proteins/metabolism , Chromosomes/genetics , DNA Damage/genetics , DNA Repair/genetics , DNA Replication/genetics , DNA, Single-Stranded/genetics , DNA, Single-Stranded/metabolism , Gene Expression Regulation, Fungal , Replication Protein A/genetics , Repressor Proteins/genetics , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae Proteins/genetics , Shelterin Complex , Telomere/genetics , Telomere-Binding Proteins/genetics , Transcription Factors/metabolism
6.
PLoS Genet ; 7(4): e1001362, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21490951

ABSTRACT

To better understand telomere biology in budding yeast, we have performed systematic suppressor/enhancer analyses on yeast strains containing a point mutation in the essential telomere capping gene CDC13 (cdc13-1) or containing a null mutation in the DNA damage response and telomere capping gene YKU70 (yku70Δ). We performed Quantitative Fitness Analysis (QFA) on thousands of yeast strains containing mutations affecting telomere-capping proteins in combination with a library of systematic gene deletion mutations. To perform QFA, we typically inoculate 384 separate cultures onto solid agar plates and monitor growth of each culture by photography over time. The data are fitted to a logistic population growth model; and growth parameters, such as maximum growth rate and maximum doubling potential, are deduced. QFA reveals that as many as 5% of systematic gene deletions, affecting numerous functional classes, strongly interact with telomere capping defects. We show that, while Cdc13 and Yku70 perform complementary roles in telomere capping, their genetic interaction profiles differ significantly. At least 19 different classes of functionally or physically related proteins can be identified as interacting with cdc13-1, yku70Δ, or both. Each specific genetic interaction informs the roles of individual gene products in telomere biology. One striking example is with genes of the nonsense-mediated RNA decay (NMD) pathway which, when disabled, suppress the conditional cdc13-1 mutation but enhance the null yku70Δ mutation. We show that the suppressing/enhancing role of the NMD pathway at uncapped telomeres is mediated through the levels of Stn1, an essential telomere capping protein, which interacts with Cdc13 and recruitment of telomerase to telomeres. We show that increased Stn1 levels affect growth of cells with telomere capping defects due to cdc13-1 and yku70Δ. QFA is a sensitive, high-throughput method that will also be useful to understand other aspects of microbial cell biology.


Subject(s)
Saccharomyces cerevisiae Proteins/metabolism , Telomere-Binding Proteins/metabolism , Telomere/metabolism , Telomere/pathology , Transcription Factors/metabolism , Gene Expression Regulation, Fungal , Models, Biological , Mutation/genetics , RNA Stability/genetics , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Telomere/genetics , Telomere-Binding Proteins/genetics , Temperature
7.
Mol Cell Biol ; 31(8): 1637-45, 2011 Apr.
Article in English | MEDLINE | ID: mdl-21321081

ABSTRACT

Replicative senescence is a permanent cell cycle arrest in response to extensive telomere shortening. To understand the mechanisms behind a permanent arrest, we screened for factors affecting replicative senescence in budding yeast lacking telomere elongation pathways. Intriguingly, we found that DNA polymerase epsilon (Pol ε) acts synergistically with Exo1 nuclease to maintain replicative senescence. In contrast, the Pol ε-associated checkpoint and replication protein Mrc1 facilitates escape from senescence. To understand this paradox, in which DNA-synthesizing factors cooperate with DNA-degrading factors to maintain arrest, whereas a checkpoint protein opposes arrest, we analyzed the dynamics of double- and single-stranded DNA (ssDNA) at chromosome ends during senescence. We found evidence for cycles of DNA resection, followed by resynthesis. We propose that resection of the shortest telomere, activating a Rad24(Rad17)-dependent checkpoint pathway, alternates in time with an Mrc1-regulated Pol ε resynthesis of a short, double-stranded chromosome end, which in turn activates a Rad9(53BP1)-dependent checkpoint pathway. Therefore, instead of one type of DNA damage, different types (ssDNA and a double-strand break-like structure) alternate in a "vicious circle," each activating a different checkpoint sensor. Every time resection and resynthesis switches, a fresh signal initiates, thus preventing checkpoint adaptation and ensuring the permanent character of senescence.


Subject(s)
DNA Polymerase II/metabolism , DNA Replication , DNA, Fungal/metabolism , Saccharomyces cerevisiae/enzymology , Cell Cycle Proteins/genetics , Cell Cycle Proteins/metabolism , DNA, Single-Stranded/biosynthesis
8.
Genetics ; 182(3): 671-84, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19380905

ABSTRACT

Telomeres are essential features of linear genomes that are crucial for chromosome stability. Telomeric DNA is usually replenished by telomerase. Deletion of genes encoding telomerase components leads to telomere attrition with each cycle of DNA replication, eventually causing cell senescence or death. In the Saccharomyces cerevisiae strain W303, telomerase-null populations bypass senescence and, unless EXO1 is also deleted, this survival is RAD52 dependent. Unexpectedly, we found that the S. cerevisiae strain S288C could survive the removal of RAD52 and telomerase at a low frequency without additional gene deletions. These RAD52-independent survivors were propagated stably and exhibited a telomere organization typical of recombination between telomeric DNA tracts, and in diploids behaved as a multigenic trait. The polymerase-delta subunit Pol32 was dispensable for the maintenance of RAD52-independent survivors. The incidence of this rare escape was not affected by deletion of other genes necessary for RAD52-dependent survival, but correlated with initial telomere length. If W303 strains lacking telomerase and RAD52 first underwent telomere elongation, rare colonies could then bypass senescence. We suggest that longer telomeres provide a more proficient substrate for a novel telomere maintenance mechanism that does not rely on telomerase, RAD52, or POL32.


Subject(s)
Rad52 DNA Repair and Recombination Protein/genetics , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae/genetics , Telomerase/genetics , Telomere/genetics , Blotting, Southern , Cell Division/genetics , DNA, Fungal/genetics , DNA, Fungal/metabolism , DNA-Directed DNA Polymerase/genetics , DNA-Directed DNA Polymerase/metabolism , Diploidy , Exodeoxyribonucleases/genetics , Exodeoxyribonucleases/metabolism , Gene Deletion , Penetrance , Phenotype , Rad52 DNA Repair and Recombination Protein/metabolism , Saccharomyces cerevisiae/growth & development , Saccharomyces cerevisiae Proteins/metabolism , Signal Transduction/genetics , Telomerase/metabolism , Telomere/enzymology , Time Factors
9.
Methods Enzymol ; 409: 285-300, 2006.
Article in English | MEDLINE | ID: mdl-16793407

ABSTRACT

Single-stranded DNA (ssDNA) is an important intermediate in many DNA repair pathways. Here we describe protocols that permit the measurement of ssDNA that has arisen in the yeast genome in vivo, in response to telomere uncapping. Yeast strains defective in DNA damage response (DDR) genes can be used to infer the roles of the corresponding proteins in regulating ssDNA production and in responding to ssDNA. Using column based methods to purify yeast genomic DNA and quantitative amplification of single-stranded DNA (QAOS) it is possible to measure ssDNA at numerous single copy loci in the yeast genome. We describe how to measure ssDNA in synchronous cultures of cdc13-1 mutants, containing a temperature sensitive mutation in an essential telomere capping protein, and in asynchronous cultures of yku70Delta mutants also defective in telomere capping.


Subject(s)
DNA Damage/genetics , DNA Repair , DNA, Single-Stranded/metabolism , Saccharomyces cerevisiae/genetics , Telomere , Base Sequence , DNA Primers
10.
Cell ; 124(6): 1155-68, 2006 Mar 24.
Article in English | MEDLINE | ID: mdl-16564010

ABSTRACT

Telomere capping is the essential function of telomeres. To identify new genes involved in telomere capping, we carried out a genome-wide screen in Saccharomyces cerevisiae for suppressors of cdc13-1, an allele of the telomere-capping protein Cdc13. We report the identification of five novel suppressors, including the previously uncharacterized gene YML036W, which we name CGI121. Cgi121 is part of a conserved protein complex -- the KEOPS complex -- containing the protein kinase Bud32, the putative peptidase Kae1, and the uncharacterized protein Gon7. Deletion of CGI121 suppresses cdc13-1 via the dramatic reduction in ssDNA levels that accumulate in cdc13-1 cgi121 mutants. Deletion of BUD32 or other KEOPS components leads to short telomeres and a failure to add telomeres de novo to DNA double-strand breaks. Our results therefore indicate that the KEOPS complex promotes both telomere uncapping and telomere elongation.


Subject(s)
Evolution, Molecular , Gene Expression Regulation, Enzymologic , Genomic Library , Saccharomyces cerevisiae Proteins/physiology , Telomere/physiology , Multiprotein Complexes/chemistry , Multiprotein Complexes/genetics , Multiprotein Complexes/metabolism , Mutation , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/physiology , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Telomerase/metabolism , Telomere-Binding Proteins/genetics , Telomere-Binding Proteins/metabolism
11.
Methods Mol Biol ; 313: 65-73, 2006.
Article in English | MEDLINE | ID: mdl-16118425

ABSTRACT

Pulsed-field gel electrophoresis (PFGE) can be used to separate the 16 budding yeast chromosomes on the basis of size. Here we describe a detailed, practical protocol that will allow a novice to perform informative PFGE experiments. We first describe the culture of yeast prior to analysis, along with details of embedding cells in agarose before removal of cell walls. We then detail the procedure to remove protein and RNA from chromosomes and how naked chromosomes are loaded into agarose gels before being subjected to electrophoresis. Finally, we describe how the separated chromosomes can be visualized and photographed.


Subject(s)
Chromosomes, Fungal/chemistry , Electrophoresis, Gel, Pulsed-Field/methods , Saccharomyces cerevisiae/chemistry , Cell Fractionation/methods , DNA Restriction Enzymes , Mycology/methods , Saccharomyces cerevisiae/growth & development , Sepharose , Staining and Labeling
12.
Cell Cycle ; 4(6): 747-51, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15970690

ABSTRACT

It is generally accepted that cells with extensive, un-repaired DNA damage can escape cell cycle arrest only by disabling checkpoint pathways and they usually perish, after several divisions, presumably due to catastrophic events on their chromosomes. Our recently discovered PAL-mechanism opens a new perspective, that some eukaryotic cells with short chromosome ends (telomeres), usually detected as DNA damage, can escape permanent cell cycle arrest (senescence) under special conditions, despite having intact checkpoints and even immortalize, despite lacking telomerase or other telomere elongation mechanisms. Here we present the first evidence that telomerase-lacking, senescent cells generate DNA damage (single stranded DNA) at internal chromosomal regions, while the telomere proximal single stranded DNA appears to be either lost or repaired. This first evidence is from the budding yeast model system. We also discuss the possible involvement of the PAL-mechanism in carcinogenesis.


Subject(s)
Chromosomes, Fungal/genetics , Telomere/genetics , Chromosome Aberrations , Humans , Recombination, Genetic , Telomerase/metabolism
13.
Genes Dev ; 18(21): 2663-75, 2004 Nov 01.
Article in English | MEDLINE | ID: mdl-15489288

ABSTRACT

It is generally assumed that there are only two ways to maintain the ends of chromosomes in yeast and mammalian nuclei: telomerase and recombination. Without telomerase and recombination, cells enter senescence, a state of permanent growth arrest. We found that the decisive role in preventing senescent budding yeast cells from dividing is played by the Exo1 nuclease. In the absence of Exo1, telomerase- and recombination-defective yeast can resume cell cycle progression, despite degradation of telomeric regions from many chromosomes. As degradation progresses toward internal chromosomal regions, a progressive decrease in viability would be expected, caused by loss of essential genes. However, this was not the case. We demonstrate that extensive degradation and loss of essential genes can be efficiently prevented through a little-studied mechanism of DNA double-strand-break repair, in which short DNA palindromes induce formation of large DNA palindromes. For the first time, we show that large palindromes form as a natural consequence of postsenescence growth and that they become essential for immortalization in the absence of telomerase activity.


Subject(s)
Recombination, Genetic , Saccharomycetales/genetics , Telomerase/metabolism , Base Sequence , Cell Proliferation , Cellular Senescence/genetics , Chromosomes, Fungal , DNA Repair/genetics , Exodeoxyribonucleases/genetics , Exodeoxyribonucleases/metabolism , Gene Expression Regulation, Fungal , Molecular Sequence Data , Repetitive Sequences, Nucleic Acid , Saccharomycetales/physiology , Telomerase/genetics , Telomere/genetics
14.
Genetics ; 166(4): 1641-9, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15126386

ABSTRACT

Telomerase-defective budding yeast cells escape senescence by using homologous recombination to amplify telomeric or subtelomeric structures. Similarly, human cells that enter senescence can use homologous recombination for telomere maintenance, when telomerase cannot be activated. Although recombination proteins required to generate telomerase-independent survivors have been intensively studied, little is known about the nucleases that generate the substrates for recombination. Here we demonstrate that the Exo1 exonuclease is an initiator of the recombination process that allows cells to escape senescence and become immortal in the absence of telomerase. We show that EXO1 is important for generating type I survivors in yku70delta mre11delta cells and type II survivors in tlc1delta cells. Moreover, in tlc1delta cells, EXO1 seems to contribute to the senescence process itself.


Subject(s)
Aging/genetics , Exodeoxyribonucleases/metabolism , Recombination, Genetic/genetics , Saccharomycetales/growth & development , Telomere/genetics , Blotting, Southern , Exodeoxyribonucleases/genetics , Genotype , Models, Genetic , Telomere/metabolism
15.
Genes Dev ; 16(15): 1919-33, 2002 Aug 01.
Article in English | MEDLINE | ID: mdl-12154123

ABSTRACT

We have examined the role of checkpoint pathways in responding to a yku70Delta defect in budding yeast. We show that CHK1, MEC1, and RAD9 checkpoint genes are required for efficient cell cycle arrest of yku70Delta mutants cultured at 37 degrees C, whereas RAD17, RAD24, MEC3, DDC1, and DUN1 play insignificant roles. We establish that cell cycle arrest of yku70Delta mutants is associated with increasing levels of single-stranded DNA in subtelomeric Y' regions, and find that the mismatch repair-associated EXO1 gene is required for both ssDNA generation and cell cycle arrest of yku70Delta mutants. In contrast, MRE11 is not required for ssDNA generation. The behavior of yku70Delta exo1Delta double mutants strongly indicates that ssDNA is an important component of the arrest signal in yku70Delta mutants and demonstrates a link between damaged telomeres and mismatch repair-associated exonucleases. This link is confirmed by our demonstration that EXO1 also plays a role in ssDNA generation in cdc13-1 mutants. We have also found that the MAD2 but not the BUB2 spindle checkpoint gene is required for efficient arrest of yku70Delta mutants. Therefore, subsets of both DNA-damage and spindle checkpoint pathways cooperate to regulate cell division of yku70Delta mutants.


Subject(s)
Antigens, Nuclear , Carrier Proteins , Cell Cycle/physiology , DNA Damage , DNA Helicases , DNA Repair , DNA, Fungal/genetics , DNA, Single-Stranded/genetics , DNA-Binding Proteins/physiology , Exodeoxyribonucleases/physiology , Genes, cdc , Nuclear Proteins/physiology , Saccharomyces cerevisiae Proteins/physiology , Saccharomyces cerevisiae/genetics , Telomere/metabolism , Base Pair Mismatch , Calcium-Binding Proteins/physiology , Cell Cycle/genetics , Cell Cycle Proteins/physiology , Checkpoint Kinase 1 , DNA, Single-Stranded/metabolism , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/deficiency , DNA-Binding Proteins/genetics , Dimerization , Fungal Proteins/physiology , Intracellular Signaling Peptides and Proteins , Ku Autoantigen , Mad2 Proteins , Nuclear Proteins/chemistry , Nuclear Proteins/deficiency , Nuclear Proteins/genetics , Protein Kinases/physiology , Protein Serine-Threonine Kinases , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae Proteins/chemistry , Saccharomyces cerevisiae Proteins/genetics , Sequence Deletion
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