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4.
Arq Bras Cir Dig ; 34(2): e1582, 2021.
Article in English, Portuguese | MEDLINE | ID: mdl-34669878

ABSTRACT

BACKGROUND: The surgical treatment of hyperhidrosis by thoracic sympathectomy has brought, in addition to symptomatic relief for many, its main adverse effect: compensatory or reflex sweating. The clipping technique in place of the sympathetic nerve section gave rise to the hope of reversibility, but the positive results showed to be quite divergent, evidencing the academic deficiency regarding the study of this phenomenon. AIM: To observe micro and macroscopic damage caused by the polymer clip on sympathetic nerve of rabbits seven days after their clipping and the findings after three weeks of clip removal. METHOD: In this experimental study, 20 rabbits were divided into two groups of 10, group 1 (clipping) and group 2 (de-clipping). The right cervical sympathetic nerve of all animals was clamped with polymeric clip, and in group 2 the nerve was unclipped seven days later. Group 1 rabbits were induced to death on the 7th postoperative day, and group 2 on the 21st after removal of the polymer clip. Macroscopic variables were: clip appearance, presence of discontinuity lesion, infection and adhesions around the nerve. H&E was used in the evaluation of the phases and degree of the inflammatory process and presence of necrosis, and picrosirius red F3BA for quantification of collagen. RESULTS: The cervical sympathetic nerve was intact, without necrosis or infection in all animals of the experiment; there were adhesions in both groups, being minimal in eight animals of each group and moderate or intense in two; the clip was completely closed in all animals at the 7th postoperative day; the inflammatory process shown was chronic, with monomorphonuclear predominance. There was no significant difference between groups regarding the intensity the inflammatory process, but the amount of collagen type I and type III was significantly higher in group 2. CONCLUSIONS: The injury caused by the polymer clip on the sympathetic nerve may be reversible, allowing functional return in the areas involved in the simulated cervical sympathectomy. Clipping of the cervical sympathetic nerve using a polymer clip does not cause discontinuity injury.


Subject(s)
Hyperhidrosis , Sympathectomy , Animals , Hyperhidrosis/surgery , Necrosis , Rabbits , Recovery of Function , Sympathetic Nervous System , Treatment Outcome
5.
ABCD (São Paulo, Impr.) ; 34(2): e1582, 2021. tab, graf
Article in English, Portuguese | LILACS | ID: biblio-1345011

ABSTRACT

ABSTRACT Background: The surgical treatment of hyperhidrosis by thoracic sympathectomy has brought, in addition to symptomatic relief for many, its main adverse effect: compensatory or reflex sweating. The clipping technique in place of the sympathetic nerve section gave rise to the hope of reversibility, but the positive results showed to be quite divergent, evidencing the academic deficiency regarding the study of this phenomenon. Aim: To observe micro and macroscopic damage caused by the polymer clip on sympathetic nerve of rabbits seven days after their clipping and the findings after three weeks of clip removal. Method: In this experimental study, 20 rabbits were divided into two groups of 10, group 1 (clipping) and group 2 (de-clipping). The right cervical sympathetic nerve of all animals was clamped with polymeric clip, and in group 2 the nerve was unclipped seven days later. Group 1 rabbits were induced to death on the 7th postoperative day, and group 2 on the 21st after removal of the polymer clip. Macroscopic variables were: clip appearance, presence of discontinuity lesion, infection and adhesions around the nerve. H&E was used in the evaluation of the phases and degree of the inflammatory process and presence of necrosis, and picrosirius red F3BA for quantification of collagen. Results: The cervical sympathetic nerve was intact, without necrosis or infection in all animals of the experiment; there were adhesions in both groups, being minimal in eight animals of each group and moderate or intense in two; the clip was completely closed in all animals at the 7th postoperative day; the inflammatory process shown was chronic, with monomorphonuclear predominance. There was no significant difference between groups regarding the intensity the inflammatory process, but the amount of collagen type I and type III was significantly higher in group 2. Conclusions: The injury caused by the polymer clip on the sympathetic nerve may be reversible, allowing functional return in the areas involved in the simulated cervical sympathectomy. Clipping of the cervical sympathetic nerve using a polymer clip does not cause discontinuity injury.


RESUMO Racional: O tratamento cirúrgico da hiperidrose pela simpatectomia torácica trouxe além do alívio sintomático para muitos, também seu principal efeito adverso: o suor compensatório ou reflexo. A técnica de clipagem do nervo simpático no lugar de sua secção deu margem à esperança de reversão do procedimento; porém, os resultados mostram-se bastante divergentes e pouco conclusivos Objetivo: Observar a lesão provocada pelo clipe de polímero em nervo simpático de coelhos sete dias após sua clipagem, comparando-a com os achados após três semanas da retirada do clipe. Método: Estudo experimental, com amostra formada por 20 coelhos, divididos em dois grupos de 10, sendo o grupo 1 chamado clipagem e o 2, desclipagem. Todos foram submetidos à clipagem do nervo simpático cervical direito com clipe polimérico, e no grupo 2 realizou-se a desclipagem sete dias após. Os coelhos do grupo 1 foram submetidos à eutanásia no 7º dia de pós-operatório, e os do grupo 2 no 21º dia após a remoção do clipe. Observou-se na macroscopia o aspecto do clipe, a presença de lesão de descontinuidade, infecção e aderências ao redor do nervo. Para estudo microscópico utilizou-se a coloração H&E na avaliação das fases, grau do processo inflamatório e presença de necrose, e a de picrosirius red F3BA para quantificação de colágeno. Resultados: O nervo simpático cervical foi identificado íntegro, sem necrose ou infecção em todos os animais do experimento; havia aderências em ambos os grupos, sendo mínimas em oito de cada grupo e moderadas ou intensas em dois; em toda a amostra o clipe encontrava-se completamente fechado no 7º dia de pós-operatório; o processo inflamatório presente foi do tipo crônico, com predomínio monomorfonuclear, não se observando diferença significativa em relação ao grau do processo inflamatório entre os grupos; porém, a quantidade de colágeno tanto do tipo I quanto do tipo III foi significativamente maior no grupo 2. Conclusões: A lesão provocada pelo clipe de polímero em nervo simpático pode ser reversível possibilitando o retorno funcional nas áreas envolvidas na simpatectomia cervical simulada. A clipagem do nervo simpático cervical com uso de clipe de polímero não causa lesão de descontinuidade.


Subject(s)
Animals , Sympathectomy , Hyperhidrosis/surgery , Rabbits , Sympathetic Nervous System , Treatment Outcome , Recovery of Function , Necrosis
8.
Rev. méd. Paraná ; 79(Supl): 5-7, 2021.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1371161

ABSTRACT

Doenças cardiovasculares são as causas mais comuns de óbitos no mundo. Técnicas de revascularização são utilizadas em casos avançados, porém frequentemente mostram complicações. Enxertos sintéticos, autólogos e heterólogos tradicionais, muitas vezes, não atendem às necessidades do paciente. O processo de descelularização representa uma via alternativa para enxertos heterólogos, sendo visado na engenharia de tecidos, devido à possibilidade de manter uma matriz orgânica bioativa e versátil, retirando apenas os agentes antigênicos. Este estudo teve o objetivo de desenvolver e validar, experimentalmente, protocolos de descelularização em vasos sanguíneos de animais e, posteriormente, avaliar o seu potencial de biocompatibilidade e recelularização in vivo. Foram extraídos segmentos arteriais da aorta torácica, aorta abdominal e carótidas comuns de coelho que foram submetidos a dois protocolos de descelularização: descelularização por método enzimático com tripsina 0,1% e pelo detergente aniônico Triton X-100 0,25%. Em conclusão, a descelularização permite a remoção de células antigênicas em enxertos vasculares, com capacidade de manter a integridade da estrutura do vaso.


Cardiovascular diseases are the most common causes of death in the world. Revascularization techniques are used in advanced cases, but they often show complications. Synthetic, autologous and traditional heterologous grafts often do not meet the patient's needs. The decellularization process represents an alternative route for heterologous grafts, being targeted in tissue engineering, due to the possibility of maintaining a bioactive and versatile organic matrix, removing only the antigenic agents. This study aimed to, experimentally, develop and validate decellularization protocols in animal blood vessels and, subsequently, evaluate their potential for biocompatibility and recellularization in vivo. Arterial segments were extracted from the thoracic aorta, abdominal aorta and common carotid arteries of rabbits that were submitted to two decellularization protocols: decellularization by enzymatic method with 0.1% trypsin and by the anionic detergent Triton X-100 0.25%. In conclusion, decellularization allows the removal of antigenic cells in vascular grafts, with the ability to maintain the integrity of the vessel structure.

9.
Rev. méd. Paraná ; 79(Supl): 17-18, 2021.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1371741

ABSTRACT

A artéria tibial anterior, originária da artéria poplítea, normalmente se ramifica abaixo da articulação do joelho, passando posteriormente ao músculo poplíteo. Desta forma, a existência da artéria tibial aberrante ocorre quando sua ramificação está alterada, podendo se formar em diferentes alturas da artéria poplítea e realizar cursos alterados em relação aos músculos e articulações. Essas variações podem aumentar o risco de lesões intra-operatórias. O objetivo deste estudo foi realizar revisão de literatura sobre o tema pouco abordado, visando promover maior conhecimento e prevenção de iatrogenia nos atos operatórios. Foi realizada busca em bases de dados eletrônicas (PubMed, Scielo e Biblioteca Virtual em Saúde) utilizando os seguintes descritores: artérias da tíbia, artéria poplítea, e variação anatômica. Para a seleção dos artigos, foram utilizados os seguintes critérios de inclusão: resultados de pesquisa com relevância ao tema proposto, por meio de leitura crítica de cada artigo selecionado e em seguida, foram realizadas comparações das informações de cada um deles. Foram selecionados 10 artigos. Em conclusão, a presença de artéria tibial anterior aberrante é alteração anatômica de baixa incidência, mas é responsável por intercorrências durante procedimentos cirúrgicos, devido à sua localização próxima à tíbia. Sugere-se realização de exames de imagens para diagnóstico pré-operatório dessa anormalidade anatômica.


The anterior tibial artery, originating from the popliteal artery, normally branches below the knee joint, passing posteriorly to the popliteal muscle. In this way, the existence of the aberrant tibial artery occurs when its branch is altered, being able to form at different heights of the popliteal artery and carry out altered courses in relation to the muscles and joints. These variations can increase the risk of intraoperative injuries. So, the proposal to this review was to promote greater knowledge and prevention of iatrogenesis in surgical procedures. A search was performed in electronic databases (PubMed, Scielo and Virtual Health Library) using the following descriptors: tibial arteries, popliteal artery, and anatomical variation. For the selection of articles, the following inclusion criteria were used: research results with relevance to the proposed topic, through a critical reading of each selected article and then comparisons of the information on each of them were carried out. 10 articles were selected. In conclusion, the presence of an aberrant anterior tibial artery is an anatomical alteration of low incidence, but it is responsible for complications during surgical procedures, due to its location close to the tibia. It is suggested to perform imaging tests for preoperative diagnosis of this anatomical abnormality.

10.
Rev. méd. Paraná ; 79(Supl): 19-20, 2021.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1371749

ABSTRACT

A tetralogia de Fallot é a cardiopatia congênita cianótica mais comum. Caracteriza-se por tétrade de malformações anatômicas que incluem estenose pulmonar, defeito do septo ventricular, hipertrofia do ventrículo direito e dextroposição da aorta (cavalgamento da aorta). O objetivo deste estudo foi analisar e descrever as alterações anatômicas cardíacas, tratamento e prognóstico de pacientes com a doença. É revisão narrativa de artigos indexados na base de dados PubMed, Google Acadêmico e LILACS utilizando os descritores "tetralogy of Fallot", "congenital heart disease", "cyanotic disease". Em conclusão, a tetralogia de Fallot é cardiopatia congênita comum e com prognóstico ruim caso não seja realizado diagnóstico e tratamento precoces. O seguimento médico contínuo e próximo ao paciente é importante para acompanhar a evolução e programar novas intervenções caso necessárias.


Tetralogy of Fallot is the most common cyanotic congenital heart disease. It is characterized by tetrads of anatomical malformations that include pulmonary stenosis, ventricular septal defect, right ventricular hypertrophy, and aortic dextroposition (aortic cavalcade). The objectives of this study were to analyze and describe cardiac anatomical changes, treatment and prognosis of patients with the disease. It was a narrative review of articles indexed in PubMed, Academic Google and LILACS databases using the descriptors "Tetralogy of Fallot", "congenital heart disease", "cyanotic disease". In conclusion, tetralogy of Fallot is a common congenital heart disease with poor prognosis if early diagnosis and treatment are not performed. Continuous medical follow-up and to be close to the patient are important to monitor the evolution and schedule new interventions if necessary.

11.
Rev. méd. Paraná ; 79(2): 43-44, 2021.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1368670

ABSTRACT

Antitopoisomerase-1 (ou Scl-70) é um autoanticorpo considerado como biomarcador da forma difusa de esclerodermia. Alguns autores o têm encontrado em pacientes com lúpus. O objetivo deste estudo foi estudar a presença do anticorpo Scl-70 em lúpus eritematoso sistêmico (SLE). É pesquisa com 94 pacientes com LES para anticorpo anti Scl-70 usando o kit comercial de ELISA Virgo™, Columbia, USA. Dados clínicos, epidemiológicos e sorológicos foram obtidos dos prontuários. Como resultado, somente 2 pacientes (2.1%) tinham anticorpos anti Scl-70 em baixos títulos. Nenhum deles tinha características de esclerodermia. Em conclusão, não se confirmam achados anteriores acerca da presença de anti Scl-70 em lúpus. Este anticorpo parece ser específico para esclerodermia.


Antitopoisomerase-1 (or Scl-70) is an autoantibody considered as a biomarker of the diffuse form of scleroderma. Some authors have found it in lupus patients. The aim of this study was to study the presence of the Scl-70 antibody in systemic lupus erythematosus (SLE). It is screened with 94 SLE patients for anti Scl-70 antibody using the commercial Virgo™ ELISA kit, Columbia, USA. Clinical, epidemiological and serological data were obtained from medical records. As a result, only 2 patients (2.1%) had anti-Scl-70 antibodies at low titers. None of them had features of scleroderma. In conclusion, previous findings regarding the presence of anti Scl-70 in lupus are not confirmed. This antibody appears to be specific for scleroderma.

12.
Rev. méd. Paraná ; 79(2): 69-72, 2021.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1368979

ABSTRACT

O uso de midazolam e fentanil é para realização de sedação consciente em endoscopia digestiva é opção; porém, o propofol vem sendo cada vez mais utilizado. O objetivo deste estudo foi identificar dos fatores de risco para prever a necessidade de uso de propofol em pacientes submetidos à endoscopia digestiva. È estudo retrospectivo em que foram avaliados 620 pacientes submetidos à endoscopia digestiva alta e colonoscopia ou ambos os procedimentos. Foram analisados variáveis epidemiológicas e doenças associadas. Em conclusão, epidemiológicas não se apresentaram como fatores de risco relacionados ao aumento da dose e uso do propofol, mas houve necessidade de aumento naqueles com doença psiquiátrica, quando não receberam sedação com midazolam, fentanil, e quando foram submetidos a procedimento combinado.


The use of midazolam and fentanyl for conscious sedation in digestive endoscopy is an option; however, propofol has been increasingly used. The aim of this study was to identify risk factors to predict the need for propofol use in patients undergoing digestive endoscopy. It is a retrospective study in which 620 patients undergoing upper digestive endoscopy and colonoscopy or both procedures were evaluated. Epidemiological variables and associated diseases were analyzed. In conclusion, epidemiological factors did not present themselves as risk factors related to the increase in the dose and use of propofol, but there was a need for those with psychiatric disease, when they did not receive sedation with midazolam, fentanyl, and when they underwent a combined procedure.

13.
Rev. méd. Paraná ; 79(2): 76-79, 2021.
Article in Portuguese | LILACS-Express | LILACS | ID: biblio-1369355

ABSTRACT

Novos preditores de sobrevida são desenvolvidos com a finalidade de auxiliar o processo de decisão do transplante hepático. Apesar disso, até o momento utilizam-se apenas o Model for End-Stage Liver Disease (MELD) como clinicamente útil. O objetivo deste estudo foi verificar a epidemiologia dos pacientes submetidos ao transplante hepático, verificar o melhor ponto de corte dos escores prognósticos BAR, SOFT, e D-MELD e avaliar se o escore D-MELD é melhor preditor de mortalidade em 1 ano comparativamente com os escores BAR e SOFT. É estudo retrospectivo transversal com base na análise dos prontuários médicos. Como conclusão, todos os escores avaliados apresentaram capacidade abaixo do ideal em predizer mortalidade em 1 ano após transplante hepático.


New survival predictors are developed in order to help the liver transplant decision process. Despite this, to date, only the Model for End-Stage Liver Disease (MELD) has been used as clinically useful. The aim of this study was to verify the epidemiology of patients undergoing liver transplantation, verify the best cutoff point for BAR, SOFT, and D-MELD prognostic scores, and assess whether the D-MELD score is a better predictor of 1-year mortality compared with the BAR and SOFT scores. It is a cross-sectional retrospective study based on the analysis of medical records. In conclusion, all scores evaluated had a suboptimal ability to predict 1-year mortality after liver transplantation.

16.
Acta cir. bras ; 31(5): 320-326, May 2016. tab, graf
Article in English | LILACS | ID: lil-783801

ABSTRACT

ABSTRACT PURPOSE : To compare ileal anastomoses in the immediate postoperative healing period after meloxicam use. METHODS: Forty two male Wistar rats were randomly divided into two groups of 21, COX and control group. To COX meloxicam in combination with morphine was given in 3 days period. Control group received only morphine during the same period. Each group was divided into three sub-groups of 7, which were euthanized at 5, 10, and 21 days postoperatively. Comparison was based in histological evaluation of collagen type I and III using sirius red, immunohistochemical through vascular endothelial growth factor and matrix metalloproteinase-9. RESULTS: Healing process in scheduled periods did not show significant differences (p>0.05) between the COX and control groups during any of the periods. CONCLUSION: The use of meloxicam in the postoperative period following ileal anastomosis did not affect healing.


Subject(s)
Animals , Male , Thiazines/pharmacology , Thiazoles/pharmacology , Wound Healing/drug effects , Anti-Inflammatory Agents, Non-Steroidal/pharmacology , Cyclooxygenase Inhibitors/pharmacology , Ileum/surgery , Postoperative Period , Time Factors , Anastomosis, Surgical , Random Allocation , Rats, Wistar , Neovascularization, Physiologic/drug effects , Matrix Metalloproteinase 9/drug effects , Matrix Metalloproteinase 9/metabolism , Models, Animal , Collagen Type I/metabolism , Collagen Type III/metabolism , Receptors, Vascular Endothelial Growth Factor/drug effects , Receptors, Vascular Endothelial Growth Factor/metabolism , Ileum/blood supply
17.
Rev. Col. Bras. Cir ; 42(6): 393-397, Nov.-Dec. 2015. tab, graf
Article in Portuguese | LILACS | ID: lil-771145

ABSTRACT

Objective: to evaluate liver regeneration in rats after partial hepatectomy of 60% with and without action diet supplemented with fatty acids through the study of the regenerated liver weight, laboratory parameters of liver function and histological study. Methods: thirty-six Wistar rats, males, adults were used, weighing between 195 and 330 g assigned to control and groups. The supplementation group received the diet by gavage and were killed after 24h, 72h and seven days. Evaluation of regeneration occurred through analysis of weight gain liver, serum aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidase, and mitosis of the liver stained with H&E. Results: the diet supplemented group showed no statistical difference (p>0.05) on the evolution of weights. Administration of fatty acids post-hepatectomy had significant reduction in gamma glutamyltransferase levels and may reflect liver regeneration. Referring to mitotic index, it did not differ between period of times among the groups. Conclusion: supplementation with fatty acids in rats undergoing 60% hepatic resection showed no significant interference related to liver regeneration.


Objetivo: avaliar a regeneração hepática em ratos submetidos à hepatectomia parcial de 60% com e sem ação de dieta suplementada com ácidos graxos ômega-3 através do estudo ponderal do fígado regenerado, parâmetros laboratoriais da função hepática e estudo histológico. Métodos: foram usados 36 ratos machos, distribuídos em dois grupos: grupo controle e grupo ômega-3. Cada um foi subdividido em mais três subgrupos com óbito em 24h, 72h e sete dias. O grupo ômega-3 recebeu água e dieta padrão suplementada com emulsão lipídica de ácidos graxos ômega-3 a 10% e o controle solução fisiológica a 0,9%. Em todos os subgrupos foi feita análise da regeneração hepática através da fórmula de Kwon, estudo da função hepática: dosagem de AST, ALT, gama-GT, bilirrubina total, bilirrubina indireta e indireta e albumina, e análise de mitose celular pela coloração de Hematoxilina-Eosina. Resultados: o grupo com dieta suplementada não apresentou diferença estatística (p>0,05) quanto à evolução dos pesos. Administração de ácidos graxos ômega-3 pós-hepatectomia mostrou que os níveis de gama-GT tiveram redução significante, podendo refletir na regeneração hepática. Na avaliação do índice mitótico não houve diferença entre os momentos estudados. Conclusão: a suplementação com ácidos graxos ômega-3 em ratos submetidos à ressecção hepática a 60% não apresentou papel expressivo relacionados à regeneração do fígado.


Subject(s)
Animals , Male , Rats , Fatty Acids, Omega-3 , Diet , Liver Regeneration , Rats, Wistar , Hepatectomy , Liver
18.
Rev Col Bras Cir ; 42(6): 393-7, 2015.
Article in English, Portuguese | MEDLINE | ID: mdl-26814992

ABSTRACT

OBJECTIVE: to evaluate liver regeneration in rats after partial hepatectomy of 60% with and without action diet supplemented with fatty acids through the study of the regenerated liver weight, laboratory parameters of liver function and histological study. METHODS: thirty-six Wistar rats, males, adults were used, weighing between 195 and 330 g assigned to control and groups. The supplementation group received the diet by gavage and were killed after 24h, 72h and seven days. Evaluation of regeneration occurred through analysis of weight gain liver, serum aspartate aminotransferase, alanine aminotransferase, gamma-glutamyltranspeptidase, and mitosis of the liver stained with H&E. RESULTS: the diet supplemented group showed no statistical difference (p>0.05) on the evolution of weights. Administration of fatty acids post-hepatectomy had significant reduction in gamma glutamyltransferase levels and may reflect liver regeneration. Referring to mitotic index, it did not differ between period of times among the groups. CONCLUSION: supplementation with fatty acids in rats undergoing 60% hepatic resection showed no significant interference related to liver regeneration.


Subject(s)
Diet , Fatty Acids, Omega-3 , Liver Regeneration , Animals , Hepatectomy , Liver , Male , Rats , Rats, Wistar
19.
Acta Cir Bras ; 27(7): 454-9, 2012 Jul.
Article in English | MEDLINE | ID: mdl-22760829

ABSTRACT

PURPOSE: To study the intraperitoneal use of polyester with collagen and polytetrafluoroethylene meshes in the correction of total ventral wall defects in rats. METHODS: Thirty two rats were evaluated and divided randomly into four groups and underwent laparotomy and preparation of total defects of the abdominal wall. Next, the correction of the defect with the intraperitoneal placement of the chosen mesh was performed. The rats were submitted to euthanasia at 30 and 90 days after surgery. Were analyzed the macroscopic adhesions and microscopic aspects, and applied stress rupture test RESULTS: All animals showed intraperitoneal adhesions in varying degrees, with no statistical significance difference. There was no difference also between groups in the evaluation of stress rupture tests. On the microscopic aspect, the A30 group had less inflammatory reaction and less formation of granulomas and foreign body reaction that the B30 group, with significant difference. CONCLUSIONS: There was no difference in intraperitoneal adhesion and tensile rupture strength among groups. Group B30 presented granulomatous inflammatory reaction at the site of mesh attachment to the wall significantly higher than the A30.


Subject(s)
Abdominal Wall/surgery , Collagen/adverse effects , Polytetrafluoroethylene/adverse effects , Surgical Mesh/adverse effects , Wound Healing/physiology , Abdominal Wall/pathology , Animals , Hernia, Ventral/surgery , Herniorrhaphy/methods , Male , Materials Testing , Random Allocation , Rats , Rats, Wistar , Tensile Strength , Time Factors , Tissue Adhesions/etiology , Tissue Adhesions/pathology
20.
Acta cir. bras ; 27(7): 454-459, jul. 2012. ilus, graf, tab
Article in English | LILACS | ID: lil-640092

ABSTRACT

PURPOSE: To study the intraperitoneal use of polyester with collagen and polytetrafluoroethylene meshes in the correction of total ventral wall defects in rats. METHODS: Thirty two rats were evaluated and divided randomly into four groups and underwent laparotomy and preparation of total defects of the abdominal wall. Next, the correction of the defect with the intraperitoneal placement of the chosen mesh was performed. The rats were submitted to euthanasia at 30 and 90 days after surgery. Were analyzed the macroscopic adhesions and microscopic aspects, and applied stress rupture test RESULTS: All animals showed intraperitoneal adhesions in varying degrees, with no statistical significance difference. There was no difference also between groups in the evaluation of stress rupture tests. On the microscopic aspect, the A30 group had less inflammatory reaction and less formation of granulomas and foreign body reaction that the B30 group, with significant difference. CONCLUSIONS: There was no difference in intraperitoneal adhesion and tensile rupture strength among groups. Group B30 presented granulomatous inflammatory reaction at the site of mesh attachment to the wall significantly higher than the A30.


OBJETIVO: Estudar comparativamente o uso intraperitoneal das telas de poliéster com colágeno e politetrafluoretileno na correção de defeitos totais da parede ventral em ratos. MÉTODOS: Foram avaliados 32 ratos distribuídos em quatro grupos aleatórios e submetidos à laparotomia e confecção de defeitos totais da parede abdominal. Em seguida, foi realizada a correção do defeito com a colocação intraperitoneal das telas. A eutanásia ocorreu aos 30 e 90 dias do pós-operatório. Foram analisadas as aderências macroscópicas, aspectos microscópicos e aplicado teste de tensão de ruptura. RESULTADOS: Todos os animais apresentaram aderências abdominais intraperitoneais em vários graus, sem significância estatística. Não houve diferença entre os grupos na avaliação dos testes de tensão de ruptura. Na análise microscópica o grupo A30 teve menor reação inflamatória e formação de granulomas, e menor reação do tipo corpo estranho quando comparado ao grupo B30, com resultados significativos. CONCLUSÕES: Não houve diferença significativa entre os grupos tanto em relação à formação de aderências intraperitoneais quanto à tensão de ruptura. O grupo B30 apresentou reação inflamatória e formação granulomatosa no local de fixação na parede significativamente maior que o grupo A30.


Subject(s)
Animals , Male , Rats , Abdominal Wall/surgery , Collagen/adverse effects , Polytetrafluoroethylene/adverse effects , Surgical Mesh/adverse effects , Wound Healing/physiology , Abdominal Wall/pathology , Hernia, Ventral/surgery , Herniorrhaphy/methods , Materials Testing , Random Allocation , Rats, Wistar , Tensile Strength , Time Factors , Tissue Adhesions/etiology , Tissue Adhesions/pathology
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