Subject(s)
Chromosome Aberrations , Chromosomes, Human, Pair 6 , Lipoma/genetics , Soft Tissue Neoplasms/genetics , Female , Humans , Middle AgedABSTRACT
Twenty-six bone marrow samples from 21 patients with Burkitt's lymphoma were examined cytogenetically after short-term culture. Clonal chromosomal changes were detected in one of 18 samples from morphologically normal marrows, and in three out of six samples with verified tumor invasion. Three patients had the translocation (8;14)(q24;q32) and one had t(2;8)(p12;q24). Two samples with suspect tumor involvement (less than 5% putative tumor cells) showed no clonal abnormalities. In two samples without morphologic bone marrow infiltration and with only normal metaphases in short-term cultures, clones with t(8;14)(q24;q32) were revealed after a longer time (3-12 weeks) spent in vitro.
Subject(s)
Bone Marrow/pathology , Burkitt Lymphoma/genetics , Cytogenetics , Adolescent , Adult , Burkitt Lymphoma/pathology , Child , Child, Preschool , Chromosome Aberrations/genetics , Chromosome Aberrations/pathology , Chromosome Disorders , Clone Cells/pathology , Female , Humans , Karyotyping , MaleABSTRACT
The malignant epithelial cells of two anaplastic EBV-carrying nasopharyngeal carcinomas (NPC) were investigated cytogenetically by Giemsa banding technique. Both tumors had a similar 3q+ marker chromosome with an involvement of band q25, either a duplication of the region q25-q27, or insertion of an unidentified segment at band q25.
Subject(s)
Carcinoma/genetics , Chromosomes, Human, 1-3 , Genetic Markers , Nasopharyngeal Neoplasms/genetics , Animals , Carcinoma/microbiology , Cell Line , Chromosome Banding , Herpesvirus 4, Human , Humans , Karyotyping , Kenya , Male , Mice , Mice, Nude , Nasopharyngeal Neoplasms/microbiology , Neoplasm TransplantationSubject(s)
Burkitt Lymphoma/genetics , Translocation, Genetic , Algeria , Burkitt Lymphoma/immunology , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Receptors, Antigen, B-Cell/analysisABSTRACT
Sister chromatid exchanges (SCE) and structural chromosome aberrations were analyzed in peripheral blood lymphocytes of 100 individuals, and correlated to age and sex. No correlation was found between the frequency of SCE and age, but older individuals had significantly more structural aberrations than younger. Females had significantly more SCE as well as structural chromosome aberrations than males. The positive correlations of SCE and structural aberrations to age and sex were also significant when these factors, as well as smoking habits, were taken into consideration in an analysis of covariance.
Subject(s)
Chromosome Aberrations , Crossing Over, Genetic , Sister Chromatid Exchange , Adolescent , Adult , Age Factors , Aged , Female , Humans , Male , Middle Aged , Sex Factors , SmokingSubject(s)
Chromosome Aberrations , Crossing Over, Genetic , Lymphocytes/ultrastructure , Sister Chromatid Exchange , Adolescent , Adult , Aged , Alcoholism/genetics , Crossing Over, Genetic/drug effects , Epoxy Resins/toxicity , Ethylene Oxide/toxicity , Female , Humans , Lymphocytes/drug effects , Male , Middle Aged , Occupations , Sister Chromatid Exchange/drug effects , Solvents/toxicityABSTRACT
A methods for chromosome analysis of malignant cells by means of fine needle aspiration biopsy has been developed. The method has some obvious advantages compared to conventional surgical biopsy. During a 1-year period 25 consecutive non-Hodgkin, non-Burkitt lymphoma biopsies from 19 patients have been studied. In 14 out of 21 lymph node biopsies chromosome analysis was successful; whereas, four biopsies of subcutaneous lymphomas were unsuccessful.
Subject(s)
Biopsy, Needle , Cytogenetics , Karyotyping/methods , Lymphoma/genetics , Humans , MetaphaseABSTRACT
Chromosomal aberrations in lymphocytes from peripheral blood were significantly more frequent in six workers from a plant manufacturing polyester resin boats (average 10.8 per 100 cells) than in six age-and sex-matched referents (5.2 per 100 cells). The contamination of the workroom air with styrene, as measured on three occasions within three years in different areas of the plant, was 50--400 mg/m3.
Subject(s)
Chromosome Aberrations , Styrenes/adverse effects , Adult , Air Pollutants, Occupational/adverse effects , Environmental Exposure , Humans , Male , Middle Aged , SwedenABSTRACT
A cytogenetic study, including prophase-prometaphase chromosome analysis, of a patient with EBV-genome-negative acute lymphocytic leukemia of B-cell type with Burkitt-type cells is presented. All bone-marrow mitoses examined had a 14q+ marker chromosome which was identified as a reciprocal 8;14 translocation of exactly the same type as in Burkitt's lymphoma.
