ABSTRACT
A new chromatographic procedure is proposed for rapid (35 min) complete fractionation of flavonoids in plant raw material; the method well discriminates between components with antioxidant activity (rutin, apigenin, quercetin, hesperidin). The method is based on reverse-phase high performance liquid chromatography using ODS-S5CN carrier and linear 10-60% pyridine elution gradient.
Subject(s)
Antioxidants/metabolism , Chemical Fractionation/methods , Chromatography, High Pressure Liquid/methods , Flavonoids/isolation & purification , Plant Extracts/chemistry , Apigenin/analysis , Flavonoids/metabolism , Hesperidin/analysis , Quercetin/analysis , Rutin/analysisABSTRACT
A simple in vitro test system based on the use of peroxide-eliminating oxidoreductases (glutathione reductase, glutathione peroxidase) is described. The system allows detection of antioxidant properties of aqueous extracts from raw medicinal plants. The system was used for testing water extract of Baikalskii-6 tea. Antioxidant and, presumably, adaptogenic activity of this tea was detected. In addition to activation of glutathione reductase and glutathione peroxidase, the preparation inhibits erythrocyte catalase and therefore should not be used for detection of antioxidant activity in in vitro screening tests.
Subject(s)
Antioxidants/analysis , Beverages/analysis , Biosensing Techniques/methods , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Plant Extracts/analysis , Plants, Medicinal/chemistry , Antioxidants/isolation & purification , Antioxidants/metabolismSubject(s)
Adenosine Triphosphate/biosynthesis , Magnesium/chemistry , Magnesium/pharmacology , Magnetics , Mitochondria/drug effects , Mitochondria/metabolism , Adenosine Diphosphate/metabolism , Animals , Creatine Kinase/metabolism , Isotopes/chemistry , Isotopes/pharmacology , Mitochondria/enzymology , Myocardium/cytology , Oxidative Phosphorylation/drug effects , RatsABSTRACT
Amino acid composition of a new Russian nephroprotective drug Nephrophyt (a complex mixture of dry plant extracts) was studied for the first time in order to validate its pharmacological effects in rats with mercuric chloride-induced nephropathy. The concentration of pyrrolidone amino acids and serine in this preparation was extremely high, which is uncommon for plant raw material. These data are in good correlation with pronounced nephroprotective effect and agree with published reports on pharmacological effects of individual amino acids and their metabolites. The prospects of the use of this preparation based on the data on its amino acid composition are discussed.
Subject(s)
Acute Kidney Injury/drug therapy , Phytotherapy , Plant Preparations/chemistry , Plant Preparations/pharmacology , Acute Kidney Injury/chemically induced , Amino Acids/analysis , Animals , Female , Male , Mercuric Chloride/toxicity , Rats , Serine/analysisABSTRACT
Antioxidant activity of a new plant preparation nephrophyt was evaluated by its effect on the rate of superoxide anion radical generation in vitro, level of Fe(2+)-induced chemiluminescence, and content of lipid peroxidation products (malonic dialdehyde and conjugated dienes) in the blood of control and experimental animals. This preparation produces a direct antioxidant effect and possesses no prooxidant activity.
