[Low-intensive laser irradiation in combined treatment of lower limbs atherosclerotic lesions]. / Nizkointensivnoe lazernoe izluchenie v kompleksnom lechenii obliteriruiushchego ateroskleroza nizhnikh konechnostei.

Seventy-one subjects entered this study. The control group consisted of 12 healthy subjects, the comparative group included 15 patients who received standard therapy of vascular diseases but without physiotherapy. The study group consisted of 44 patients whose treatment was supplemented with laser irradiation. Angiography, ultrasonic dopplerography, laser flowmetry, oxygenometry were applied for control of treatment efficacy. Regional ischemia was evaluated with detection of pO2 of foot. LT increased oxygenation of foot soft tissues in patients with low primary pO2 and decreased in ones with higher. As a result the number of patients with low pO2 (0 < pO2 < 20) decreased from 13.7 to 4.5%, with middle pO2 (20 < pO2 < 40) increased from 27.3 to 50.0%, with high pO2 (pO2 = 40) decreased from 59.0 to 45.5%. Redistribution in favor of 20 < pO2 < 40 is regarded as normalizing effect of LT. It is concluded that LT increases oxygenation of foot soft tissues in patients with low primary pO2 and decreased in ones with higher.

[Use of data bank on tuberculosis patients and of computer program for clinical and immunological analysis]. / Ispol'zovanie banka dannykh o bol'nykh tuberkulezom i komp'iuternoí programmy dlia kliniko-immunologicheskogo analiza.

Three hundred and twenty patients with pulmonary tuberculosis were clinically and immunologically compared. The findings support the significance and prospects for using a personal computer while making clinical and laboratory comparisons in different groups of patients for a prompt and objective analysis of available data. Comparative evaluation of the informative value of immunologic tests have indicated that in relation to the major clinical parameters of a tuberculous process, differences are great in those characterizing both T- and B-cell, and specific antimycobacterial immunity. The clinical and immunological findings regarding the specific features of a tuberculosis process may serve as the basis for multidimensional analysis and development of criteria for computer-aided clinical and immunological diagnosis of tuberculosis.

[Laser and chemotherapy and the immune status of patients with fibrous-cavernous tuberculosis]. / Khimiolazerterapiia i immunnyi status bol'nykh fibrozno-kavernoznym tuberkulezom.

Treatment outcomes were analysed for 245 patients with fibro-cavernous tuberculosis (FCT). 103 of them were exposed to adjuvant low-energy laser radiation. The addition of the radiation to combined treatment of FCT patients contributes to more efficient treatment. The benefit lies in less severe intoxication, infiltration, discontinuation of bacterial discharge, more intensive normalisation of T-cell immunity, arrest of active inflammation, diminished antigenemia, normalisation of specific immunological reactivity.

[Identification of mycobacteria in sputum by determining antigens in the culture medium]. / Identifikatsiia mikobakterii v mokrote s pomoshchíu opredeleniia antigenov v kulturalnoi zhidkosti.

Antigens to M. tuberculosis in culture fluid of sputum samples were compared to bacterioscopy and inoculation results for 64 new-onset cases of tuberculosis and 60 chronics with bronchitis. The authors used diagnostic preparations of monoclonal and polyclonal antibodies to M. tuberculosis. The use of the above preparations in the enzyme immunoassay reduced the time of M. tuberculosis detection in the patients' sputum and increased the frequency of the bacteria registration against standard methods of bacterioscopy and inoculation.

[The level and spectrum of antituberculosis antibodies in BCG-vaccinated mice and in mice and rabbits infected with a virulent mycobacterial culture]. / Uroven' i spektr protivotuberkuleznykh antitel u vaktsnirovannykh BTsZh myshei i zarazhennykh virulentnoi kul'turoi mikobakterii myshei i krolikov.

The authors examined CBA mice infected with a virulent culture (H37 Rv), BCG-vaccinated mice and intact mice and rabbits infected with a virulent culture Bovinus-8 after preliminary sensitization. It was established that the level of antituberculous antibodies in the enzyme-linked immunoassay was higher in H37 Rv infected mice than in BCG-vaccinated animals (regardless of the used antigen, the ultrasonic disintegrator BCG or H37 Rv). The spectrum of antituberculous antibodies was different in the infected and vaccinated mice, the infected mice showed additional bands in the 20 kD area. The level of antituberculous antibodies was higher in rabbits with a limited process than in those with a disseminated process. With the latter, antibodies to antigenic determinants that had lower molecular weight (25-35 kD) were detectable.

[Level and spectrum of tuberculosis antibodies in mice sensitive and resistant to tuberculosis]. / Uroven' i spektr protivotuberkuleznykh antitel u chuvstvitel'nykh i rezistentnykh k tuberkulezu myshei.

The level and spectrum of antituberculous antibodies (AA) were assessed in mice and their hybrids sensitive or resistant to tuberculosis. The AA level in resistant animals (CBA) and hybrids (CBA x C57Bl/6) F1 surpassed that in the sensitive mice (C57Bl/6). The spectrum in resistant and hybrid mice appeared more varied than that in the sensitive animals. Resistant mice had bands in the range 15-20 kD.

[Determination of antibodies in blood serum of patients with pulmonary tuberculosis using mycobacterial antigen affinity isolation]. / Opredelenie antitel v syvorotke krovi u bol'nykh tuberkulezom legkikh s ispol'zovaniem affinno vydelennogo mikobakterial'nogo antigena.

Possible use of radio- and enzyme immunoassays (RIA and EIA, respectively) in serodiagnosis of tuberculosis with antigen BCG isolated by affinity was studied in comparison to the use of the routine antigen PPD. The specific IgG antibodies were determined in serum specimens of 150 patients with various forms of pulmonary tuberculosis and 50 healthy donors. A statistically reliable difference in the efficiency of tuberculosis serodiagnosis (high levels of specificity and sensitivity) by the RIA and EIA with using antigen BCG isolated by affinity was observed.

[Antitubercular antibodies to antigens of various molecular weights in patients with active pulmonary tuberculosis]. / Protivotuberkuleznye antitela k antigenam raznoi molekuliarnoi massy u bol'nykh s aktivnym tuberkulezom legkikh.

Antibodies were determined in 72 tuberculous patients and 27 healthy donors using enzyme immunoassay (EIA) and immunoblotting (IB). It was shown that the levels of the antibodies to BCG and H37Rv sonicates were lower in the patients with disseminated tuberculosis than in the patients with fibrocavernous or infiltrative tuberculosis. Significant differences in the antibody spectra in the patients with different forms of tuberculosis were also shown. Thus, in the patients with disseminated tuberculosis IB with BCG and H37Rv sonicates revealed only single bands in the region of the reaction with the antigens of low molecular weights. In the patients with infiltrative tuberculosis the reaction with H37Rv or BCG sonicates most frequently revealed the antibodies to the antigen determinants with molecular weights of 50, 30, 41, 54, 52 and 60.43 or 45.5, 40.5, 57 and 52 kD, respectively. In the patients with fibrocavernous tuberculosis the reaction with H37Rv or BCG sonicates most frequently revealed the antibodies to the antigen determinants with molecular weights of 54, 42, 48, 30 and 13.5 or 24, 57, 37 and 14.5 kD respectively.

[Immunological tolerance and antituberculosis immunity]. / Immunologicheskaia tolerantmost' i protivotuberkuleznyi immunitet.

Marked tuberculosis immunity and resistance to tuberculous infection during infecting with virulent culture of mycobacteria H(37)Rv were studied in experimental immunologic tolerance indiced in CBA mice by administering a large dose of the polyantigen BCG and the immunodepressant cyclophosphane. After BCG vaccination the tolerant animals do not develop tuberculosis cellular immunity (in accordance to blast transformation and skin tuberculin tests), with the protective effect of vaccination not brought up in a group of tolerant mice.

In vitro and in viro effects of the fifth thymosin fraction.

Using the 5th fraction of thymosin, it was possible to enhance the capacities of peripheral blood lymphocytes to form rosettes with sheep erythrocytes and to respond to PHA in those cases of cancer or sarcoidosis in which these functions were depressed. Moreover, the 5th thymosin fraction increased the resistance of CBA mice to infection with virulent H37Rv mycobacteria and augmented their ability to acquire delayed hypersensitivity.