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Appl Microbiol ; 26(1): 116-7, 1973 Jul.
Article in English | MEDLINE | ID: mdl-4146938

ABSTRACT

One hundred twenty-five strains of Diplococcus pneumoniae and one hundred twenty-five strains of other alpha-hemolytic streptococci were planted on horse red cell-Brucella agar, and a disk of methicillin was placed on each inoculated plate. All strains were incubated anaerobically at 37 C for 24 h and then exposed to air at 6 C for 48 h. All strains of pneumococci produced a ring of beta-hemolysis surrounding the zone of inhibition produced with the methicillin disk, whereas no strain of alpha-hemolytic streptococci produced beta-hemolysis. It is suggested that this new criterion is useful for the differentiation of pneumococci from other alpha-hemolytic streptococci in those cases where other characteristics were insufficient for an unequivocal identification.


Subject(s)
Bacteriological Techniques , Streptococcus pneumoniae/isolation & purification , Streptococcus/isolation & purification , Aerobiosis , Agar , Air , Animals , Erythrocytes , Hemolysin Proteins/analysis , Hemolysin Proteins/biosynthesis , Horses , Humans , Methicillin , Methods , Sputum/microbiology , Streptococcus/classification , Streptococcus/metabolism
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