ABSTRACT
The ADAM (A Disintegrin and Metalloprotease) family of transmembrane proteins plays important roles in embryogenesis and tissue formation based on their multiple functional domains. In the present study, for the first time, the expression patterns of the premature and the active forms of six members of the ADAM proteins - ADAM9, ADAM10, ADAM12, ADAM17, ADAM22 and ADAM23 - in distinct parts of the developing chicken brain were investigated by quantitative Western blot analysis from embryonic incubation day (E) 10 to E20. The results show that the premature and the active forms of various ADAM proteins are spatiotemporally regulated in different parts of the brain during development, suggesting that the ADAMs play a very important role during embryonic development.
Subject(s)
ADAM Proteins/genetics , Brain/embryology , Brain/metabolism , Chickens/metabolism , Gene Expression Profiling , Gene Expression Regulation, Developmental , ADAM Proteins/chemistry , ADAM Proteins/metabolism , Amino Acid Sequence , Animals , Antibody Specificity/immunology , Blotting, Western , Chick Embryo , Embryonic Development/genetics , Isoenzymes/genetics , Isoenzymes/metabolism , Molecular Sequence Data , Organ Specificity/genetics , Peptides/chemistryABSTRACT
ADAM19 (also named meltrin ß) is a member of the ADAM (a disintegrin and metalloprotease) family of metalloproteases and is involved in morphogenesis and tissue formation during embryonic development. In the present study, chicken ADAM19 is cloned by reverse transcription-polymerase chain reaction and identified by sequencing. Its expression patterns in different parts of the developing chicken embryo are investigated by Western blot analysis and immunohistochemistry. Results show that ADAM19 protein is widely expressed in chicken embryos. It is detectable in the central nervous system, including the brain, spinal cord, cochlea, and retina. Furthermore, ADAM19 protein is also found in other tissues and organs such as digestive organs, the thymus, the lung bud, the dorsal aorta, the kidney, the gonad, muscles, and in the feather buds. All these data suggest that ADAM19 plays an important role in the embryonic development of chicken.
Subject(s)
ADAM Proteins/biosynthesis , Chickens/metabolism , Embryonic Development/physiology , Gene Expression Regulation, Developmental/physiology , Gene Expression Regulation, Enzymologic/physiology , ADAM Proteins/genetics , Animals , Chick Embryo , Chickens/genetics , Organ Specificity/physiologyABSTRACT
The expression patterns of five members of the ADAM (a disintegrin and metalloprotease) family including ADAM9, ADAM10, ADAM17, ADAM22, and ADAM23 were analyzed in different anatomical structures of the developing chicken cochlea by in situ hybridization and immunohistochemistry. Results show that ADAM9, ADAM10, and ADAM17 are widely expressed in the sensory epithelium of the basilar papilla, by homogene cells, spindle-shaped cells, and acoustic ganglion cells, and in the tegmentum vasculosum, each with a different pattern. ADAM22 expression is restricted to spindle-shaped cells and acoustic ganglion cells, while ADAM23 is prominently expressed by hair cells and acoustic ganglion cells. Furthermore, ADAM10 protein is coexpressed with several members of the classic cadherins, including cadherin-7, N-cadherin, and R-cadherin in distinct anatomical regions of the cochlea except for acoustic ganglion cells. The expression of the ADAMs in the developing cochlea suggests a contribution of the ADAMs to the development of distinct cochlear structures.
Subject(s)
ADAM Proteins/analysis , Cochlea/growth & development , Animals , Cadherins/analysis , Cochlea/chemistry , Gene Expression , Gene Expression Regulation, Developmental , Organ of Corti/chemistry , Tissue DistributionABSTRACT
The expression patterns of seven members of the ADAM (a disintegrin and metalloprotease) family, including ADAM9, ADAM10, ADAM12, ADAM13, ADAM17, ADAM22, and ADAM23, were analyzed in the developing chicken lumbar spinal cord by in situ hybridization and immunohistochemistry. Results show that each individual ADAM is expressed and regulated spatiotemporally in the lumbar cord and its surrounding tissues. ADAM9, ADAM10, ADAM22, and ADAM23 are expressed predominantly by motoneurons in the motor column and by sensory neurons in the dorsal root ganglia, each with a different expression pattern. ADAM12 and ADAM13 are mainly expressed in the meninges around the lumbar cord and in the condensed sheets of chondroblasts around the vertebrae. ADAM17 expression is strong in the ventricular layer and limited to early stages. The differential expression of the ADAMs in the lumbar cord suggests that the ADAMs play a regulatory role in development of the spinal cord.
Subject(s)
ADAM Proteins/genetics , Chick Embryo , Spinal Cord/embryology , ADAM Proteins/metabolism , Animals , Blood Vessels/embryology , Blood Vessels/metabolism , Chick Embryo/metabolism , Dermis/embryology , Dermis/metabolism , Ganglia, Spinal/embryology , Ganglia, Spinal/metabolism , Ganglia, Sympathetic/embryology , Ganglia, Sympathetic/metabolism , Gene Expression Profiling , Gene Expression Regulation, Developmental , Models, Biological , Multigene Family/genetics , RNA, Messenger/analysis , RNA, Messenger/metabolism , Spinal Cord/metabolismABSTRACT
During chicken spinal cord development, cadherin-20 is expressed by motor neurons of the lateral motor column and other cells in the basal and alar plates. To investigate the regulation of cadherin-20 expression, Sonic hedgehog (Shh) signaling was altered in chicken spinal cord and hindbrain by in-vivo electroporation. Our results show that, at an early embryonic stage 12, Shh induces cadherin-20 expression by motor neurons. Later, at stage 24 when the sorting of motor neuron pools begins, cadherin-20 expression is induced in the neural progenitors of the ventricular zone but not in motor neurons. Blockage of Shh signaling inhibits cadherin-20 expression in the motor column. Therefore, cadherin-20 expression in motor neurons is regulated by Shh in a time-dependent manner.