Human T-lymphotropic virus 1/2 infection among prisoners of a major penitentiary complex of Goiás State, Central-West Brazil.

Introduction: Studies on human T-lymphotropic virus 1/2 (HTLV-1/2) infection are scarce in incarcerated population. Therefore, this study estimated the prevalence of HTLV-1/2 infection among prisoners of the major penitentiary complex of Goiás State, Central-West Brazil, comparing it with available data from other Brazilian regions. Methods: A cross-sectional study was conducted with 910 prisoners of the major penitentiary complex in the State of Goiás, Central-West Brazil. All participants were interviewed, and their serum samples were tested for anti-HTLV-1/2 using an enzyme-linked immunosorbent assay (ELISA; Murex HTLV-I + II, DiaSorin, Dartford, UK). Seropositive samples were submitted for confirmation by a line immunoassay (INNO-LIA HTLV I/II, Fujirebio, Europe N.V., Belgium). Results: The majority of participants were males (83.1%), between 25 and 39 years old (56.1%; mean age: 31.98 years), self-reported brown ethnicity (56.2%) and reported 9 years or less of formal education (41.4%). Most reported using non-injectable illicit drugs and various sexual behaviors that present risk for sexually transmitted infections (STIs). The prevalence of anti-HTLV-1/2 was 0.33% (95% CI: 0.07-0.96), HTLV-1 (0.22%) and HTLV-2 (0.11%). The two HTLV-1 seropositive prisoners reported high-risk sexual behaviors, and the HTLV-2 seropositive individual was breastfed during childhood (> 6 months) by her mother and three other women. Conclusion: These data revealed a relatively low seroprevalence of HTLV-1/2 in prisoners in Central-West Brazil, and evidence of HTLV-1 and HTLV-2 circulation in the major penitentiary complex of Goiás State. Given the prevalence of high-risk sexual behaviors, there is a crucial need to intensify education and health programs in prisons to effectively control and prevent HTLV-1/2 and other STIs.

Pain characterization in patients with Parkinson's disease.

BACKGROUND: Patients with Parkinson's disease (PD) often report chronic pain, which is one of the most complex non-motor symptoms. Therefore, this study aims to review the literature on the characteristics of pain in patients with PD. METHODS: A systematic literature review was conducted following MOOSE recommendations. Observational studies reporting pain in patients with PD were included. No time restrictions were applied, but studies in Portuguese, Spanish, and English were considered. The search was performed in PubMed®, LILACS, and SciELO databases. RESULTS: Twenty-six articles of observational studies were identified, reporting an average pain prevalence of 67.36%, emphasizing the significance of this symptom in the PD population. Pain was reported in various body regions, including lower limbs, upper limbs, lumbar spine, cervical spine, and other joints. Pain classification varied, encompassing musculoskeletal pain, PD-related pain, neuropathic pain, and dystonic pain, among others. DISCUSSION: Pain in patients with PD is a prevalent and multifactorial condition, significantly impacting patients' quality of life. CONCLUSION: Heterogeneity in data across included studies was observed, highlighting the need for additional research to elucidate the underlying mechanisms of pain in patients with PD and develop effective therapeutic strategies to address this symptom and improve the quality of life for individuals living with the disease.

Relationship between Na/K-ATPase in thawed sperm and fertility of Angus bulls.

Since bull fertility prediction remains challenging, the identification of potential fertility markers is important considering the economic benefits to the livestock industry. The main goal of this study was to determine the Na/K-ATPase activity and expression in thawed sperm of high (HF)- and low-fertility (LF) Angus bulls. Samples from three different batches/bulls with HF (n = 4) and LF (n = 4) were used. The Na/K-ATPase activity was determined after thawing, whereas sperm kinematics, membrane integrity, and expression of Na/K-ATPase on sperm surface were evaluated immediately post-thaw and after 120 minutes of incubation. Within the same incubation time, there was no difference on sperm membrane integrity, kinematics, and the expression of Na/K-ATPase on the sperm surface between HF and LF bulls. Kinematic parameters of LIN and VCL were not influenced by incubation time in samples from HF and LF, respectively. A tendency (P = 0.06) of higher Na/K-ATPase enzymatic activity for sperm of HF bulls compared to LF bulls was observed (0.49 ± 0.07 and 0.32 ± 0.06, respectively). In conclusion, Na/K-ATPase activity and expression in thawed sperm from Angus bulls are not related to the fertility index after fixed-time artificial insemination. However, sperm kinematics related to hyperactivation might indicate higher sperm cryotolerance for HF bulls.

Homozygous CDH2 variant may be associated with hypopituitarism without neurological disorders.

Context: Congenital hypopituitarism is a genetically heterogeneous condition. Whole exome sequencing (WES) is a promising approach for molecular diagnosis of patients with this condition. Objectives: The aim of this study is to conduct WES in a patient with congenital hypopituitarism born to consanguineous parents, CDH2 screening in a cohort of patients with congenital hypopituitarism, and functional testing of a novel CDH2 variant. Design: Genomic DNA from a proband and her consanguineous parents was analyzed by WES. Copy number variants were evaluated. The genetic variants were filtered for population frequency (ExAC, 1000 genomes, gnomAD, and ABraOM), in silico prediction of pathogenicity, and gene expression in the pituitary and/or hypothalamus. Genomic DNA from 145 patients was screened for CDH2 by Sanger sequencing. Results: One female patient with deficiencies in growth hormone, thyroid-stimulating hormone, adrenocorticotropic hormone, luteinizing hormone, and follicle-stimulating hormone and ectopic posterior pituitary gland contained a rare homozygous c.865G>A (p.Val289Ile) variant in CDH2. To determine whether the p.Val289Ile variant in CDH2 affects cell adhesion properties, we stably transfected L1 fibroblast lines, labeled the cells with lipophilic dyes, and quantified aggregation. Large aggregates formed in cells expressing wildtype CDH2, but aggregation was impaired in cells transfected with variant CDH2 or non-transfected. Conclusion: A homozygous CDH2 allelic variant was found in one hypopituitarism patient, and the variant impaired cell aggregation function in vitro. No disease-causing variants were found in 145 other patients screened for CDH2 variants. Thus, CDH2 is a candidate gene for hypopituitarism that needs to be tested in different populations. Significance statement: A female patient with hypopituitarism was born from consanguineous parents and had a homozygous, likely pathogenic, CDH2 variant that impairs cell aggregation in vitro. No other likely pathogenic variants in CDH2 were identified in 145 hypopituitarism patients.

Prevalence of overt and occult hepatitis B virus infection among an incarcerated population of Central-Western Brazil.

Brazilian prison complexes are characterized by overcrowded cells and precarious conditions, leading to chronically low vacancy. Brazilian studies involving overt and occult infection (OBI) in this population are still scarce despite the vulnerability of people deprived of liberty to hepatitis B. Therefore, this study estimated the prevalence of HBV infection (overall and OBI) in individuals deprived of liberty in prisons in Central-Western Brazil. In addition, factors associated with HBV infection were evaluated. This cross-sectional study was conducted with a total of 1083 prisoners who were tested for serological hepatitis B markers and HBV DNA from 2017 to 2020. Factors associated with lifetime HBV infection were investigated using logistic regression. An overall prevalence of HBV infection of 10.1% (95% CI: 8.42-12.11) was detected. Only 32.8% (95% CI: 30.08-35.76) had isolated anti-HBs positivity (serological evidence of HBV vaccination). Indeed, more than half of the population was susceptible to HBV infection (57.1%; 95% CI: 54.15-60.13). HBV DNA was detected in one HBsAg-positive sample (n=1/9; 11%). Also, HBV DNA was detected in five HBsAg-negative samples (n=5/1074), resulting in a prevalence of 0.5% (95% CI: 0.15-1.08) for occult infection. After the multivariate analysis, sexual intercourse with a partner living with HIV was a predictor independently associated with HBV exposure (OR: 4.3; 95% CI: 1.26-14.55; p<0.020). These data demonstrate the need for preventive measures, mainly aimed at health education and better strategies for hepatitis B screening to control this infection in prisons more effectively.

Tratamento ortodôntico de paciente portador de síndrome de Treacher Collins ­ relato de caso / Orthodontic treatment of a patient with Treacher Collins syndrome ­ case report

Resumo Este trabalho é uma descrição de um caso clínico de paciente portador de Síndrome de Treacher Collins (STC). O paciente em questão é menor de idade e foi submetido a tratamento ortodôntico interceptivo de má oclusão de classe II, característica da síndrome devido à retrognatia, no Centro de Atenção e Pesquisa em Anomalia Craniofacial (CEAPAC), Cascavel ­ PR. As disostoses faciais são um conjunto de anomalias raras do esqueleto craniofacial, a mais comumente descrita é a STC, que é uma doença rara, sem predisposição por sexo ou raça. Os sintomas e a severidade desta síndrome diferem de indivíduo para indivíduo, mesmo entre membros da mesma família. Suas características comuns são as anormalidades dos pavilhões auriculares, hipoplasia dos ossos da face, obliquidade antimongolóide das fendas palpebrais com coloboma palpebral inferior e fissura palatina e o principal problema anatômico é a hipoplasia do terço médio da face e o hipodesenvolvimento da mandíbula e mento, o que leva o paciente portador desta síndrome apresentar por suas características faciais uma má oclusão de classe II muitas vezes associadas à mordida aberta, além de outros problemas orais como, por exemplo, as patologia das glândulas salivares, respiração bucal e apinhamento dentário. O tratamento ortodôntico intercepetivo do menor, embora após terminado tenha permanecido com má oclusão de classe II, obteve mudanças em parâmetros cefalométricos e faciais do paciente.(AU)

Abstract This work is a description of a clinical case of a patient with Treacher Collins Syndrome (CTS). The patient in question is younger and underwent interceptive orthodontic treatment of class II malocclusion, characteristic of the syndrome due to retrognathia, at the Center for Attention and Research in Craniofacial Anomaly (CEAPAC), Cascavel ­ PR. Facial dysostosis is a set of rare anomalies of the craniofacial skeleton, the most commonly described being CTS, it is a rare disease, without predisposition by sex or race. The symptoms and severity of this syndrome differ from individual to individual, even among members of the same family. Its common features are auricular pavilion abnormalities, facial bone hypoplasia, antimongoloid obliquity of the palpebral fissures with lower palpebral coloboma and cleft palate, and the main anatomical problem is hypoplasia of the middle third of the face and hypodevelopment of the mandible and chin, which Due to their facial characteristics, patients with this syndrome have a Class II malocclusion, often associated with an open bite, in addition to other oral problems such as salivary gland pathology, mouth breathing and dental crowding. The minor's interceptive orthodontic treatment, although after it ended, he remained with class II malocclusion, resulted in changes in the patient's cephalometric and facial parameters.(AU)

PET-Saúde Interprofissionalidade e a disponibilidade dos estudantes para a aprendizagem interprofissional / PET-Health Interprofessionality and the readiness of students for interprofessional learning

Resumo A interprofissionalidade foi tema central do nono edital do Programa de Educação pelo Trabalho para a Saúde. O objetivo deste estudo foi descrever a disponibilidade dos estudantes que participaram do programa para a aprendizagem interprofissional. A pesquisa, de abordagem quantitativa e de perspectiva descritivo-exploratória, coletou dados através da readiness for interprofessional learning scale, adaptada para o português do Brasil. Os resultados indicam que há disponibilidade para o trabalho em equipe e a colaboração. Também foram encontrados percentuais significativos relacionados à identidade profissional, o que indica disponibilidade para a aprendizagem interprofissional, embora aspectos relacionados à competição tenham aparecido através de percentuais menores de concordância em relação à aprendizagem compartilhada e interdependência das práticas profissionais. Para a atenção centrada no usuário também foram encontrados resultados que indicam boa disponibilidade entre os participantes; porém, aspectos relacionados à colaboração do usuário na produção dos serviços de saúde ainda expressam a tendência de uma lógica centrada nos profissionais. Conclui-se que há disponibilidade positiva para a educação interprofissional, que precisa ser cada vez mais estimulada nas políticas e estratégias de reformas curriculares.

Abstract Interprofessionality was the central theme of the ninth call for proposals of the Education by Working for Health Program. The objective of this study was to describe the readiness of students who participated in the program for interprofessional learning. The research, of a quantitative approach and descriptive-exploratory perspective, collected data by applying the readiness for interprofessional learning scale (RIPLS), adapted for Brazilian Portuguese. The results indicate an important readiness for teamwork and collaboration. Relevant percentages were also found regarding professional identity, indicating good readiness for interprofessional learning, although aspects related to competition appeared in lower percentages of agreement regarding shared learning and interdependence of professional practices. For user-centered care, results were also found indicating good readiness among participants; however, aspects related to the collaboration of the user in the production of health services still express the tendency of a professional-centered logic. In conclusion, there is a positive readiness for interprofessional education, which needs to be increasingly stimulated in policies and strategies of curricular reforms.

Comparison of the sedative effects of three nalbuphine doses, alone or combined with acepromazine, in dogs.

OBJECTIVE: To compare sedative, cardiopulmonary, and adverse effects of 3 nalbuphine doses, administered alone or in combination with acepromazine, in dogs. ANIMALS: 6 healthy dogs. PROCEDURES: Dogs were administered nalbuphine (1.0, 1.5, or 2.0 mg/kg, intravenously [IV]) combined with physiologic saline solution (1 mL, IV; treatments SN1.0, SN1.5, and SN2.0, respectively) or acepromazine (0.05 mg/kg, IV; treatments AN1.0, AN1.5, and AN2.0, respectively) in random order, with a 1-week washout interval between treatments. Sedation scores, heart rate, mean arterial pressure, respiratory rate, and rectal temperature were recorded before and 20 minutes after administration of saline solution or acepromazine (T0), and nalbuphine was administered at T0. Measurements were repeated 15, 30, 60, 90, and 120 minutes after nalbuphine administration. RESULTS: Treatments SN and AN resulted in at least 120 minutes of mild sedation and 60 minutes of moderate sedation, respectively. Sedation scores were greater for treatments AN1.0, AN1.5, and AN2.0 at various times, compared with scores for treatments SN1.0, SN1.5, and SN2.0, respectively. Administration of nalbuphine alone resulted in salivation and panting in some dogs. CLINICAL RELEVANCE: All nalbuphine doses promoted mild sedation when administered alone, and moderate sedation when combined with acepromazine. Greater doses of nalbuphine did not increase sedation scores. All treatments resulted in minimal changes in heart rate, respiratory rate, rectal temperature, and mean arterial pressure. Nalbuphine alone resulted in few adverse effects.

Ventilatory Muscle Training for Early Cardiac Rehabilitation Improved Functional Capacity and Modulated Vascular Function of Individuals Undergoing Coronary Artery Bypass Grafting: Pilot Randomized Clinical Trial.

Background: Cardiac rehabilitation with aerobic exercises is the first strategy for nonpharmacological treatment in the postoperative period of individuals undergoing coronary artery bypass grafting (CABG) to improve functional capacity and vascular health. However, other exercise modalities remain uncertain regarding the same benefits. Objectives: Evaluation of the effect of different modalities of exercise, such as early cardiac rehabilitation on subjects submitted to CABG in the six-minute walk test (6-MWT) and on the percentage of flow-mediated dilatation (FMD) of the brachial artery. Methods: A randomized clinical trial in which 15 patients (62.7 ± 6.7 years) who underwent CABG were randomly assigned to the following groups: isometric (IG, Handgrip Jamar®), ventilatory muscle training (VG, PowerBreathe®) and control (CG, conventional respiratory and motor physiotherapy). All patients were attended to physically twice a day (20 min/session) for a consecutive week after the CABG (hospital admission). Functional capacity was assessed by 6-MWT and endothelial function was assessed through the technique of FMD, before and after (~7 days) admission to CABG. The doppler ultrasound videos were analyzed by Cardiovascular Suite® software (Quipu, Pisa, Italy) to measure %FMD. Statistics: Generalized estimation equation, followed by Bonferroni post hoc (p < 0.05). Results: Systolic, diastolic and mean arterial pressure (SBP/DBP/MAP, respectively) were 133, 76 and 95 mmHg. The groups presented walking meters (m) distance before and after intervention of: IGbasal 357.80 ± 47.15 m vs. IGpost 306.20 ± 61.63 m, p = 0.401 (+51 m); VGbasal 261.50 ± 19.91 m vs. VGpost 300.75 ± 26.29 m, p = 0.052 (+39 m); CG basal 487.83 ± 83.23 m vs. CGpost 318.00 ± 31.08, p = 0.006 (−169 m). %FMD before and after intervention was IGbasal 10.4 ± 4.8% vs. IGpost 2.8 ± 2.5%, p = 0.152; VGbasal 9.8 ± 5.1% vs. VGpost 11.0 ± 6.1%, p = 0.825; CGbasal 9.2 ± 15.8% vs. CGpost 2.7 ± 2.6%, p = 0.710 and resting mean basal blood flow was IGbasal 162.0 ± 55.0 mL/min vs. IGpost 129.9 ± 63.7 mL/min, p = 0.662; VGbasal 83.74 ± 12.4 mL/min vs. VGpost 58.7 ± 17.1 mL/min, p = 0.041; CGbasal 375.6 ± 183.7 mL/min vs. CGpost 192.8 ± 115.0 mL/min, p = 0.459. Conclusions: Ventilatory muscle training for early cardiac rehabilitation improved acute functional capacity and modulated mean flow of individuals undergoing CABG.

PMMA Microcapsules for the Inactivation of SARS-CoV-2.

Surface disinfection currently plays a decisive role in the epidemiological situation caused by the SARS-CoV-2 coronavirus. However, most disinfection products available on the market have a high evaporation rate and only an immediate action and not continuous, creating the need for a high frequency of disinfection. To overcome this limitation, in the present work, poly(methyl methacrylate) (PMMA) microcapsules were developed with an active agent (hydrogen peroxide) encapsulated, which has the ability to inactivate/neutralize the SARS-CoV-2 virus. PMMA-H2O2 microcapsules have a spherical shape and a smooth structure with low porosity and were successfully attached to nonwoven fabrics, as observed from scanning electron microscopy. The thermogravimetric analysis shows that PMMA-H2O2 microcapsules have high thermal stability and can increase the stability of H2O2. Nonfabric substrates functionalized with PMMA-H2O2 microcapsules were tested by a highly sensitive and specific reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR)-based method to evaluate antiviral activity through the degradation of SARS-CoV-2 deoxyribonucleic acids. The highest percentage of viral nucleic acid elimination was obtained when exposing the viral sample for 1 h to PMMA-H2O2 microcapsules, resulting in an elimination of >97% of the coronavirus. In addition, the microcapsules are stable over a period of three weeks and retain the ability to eliminate SARS-CoV-2. Hence, it is demonstrated that this microcapsule system is efficient for SARS-CoV-2 elimination and inherent surface disinfection.

Microencapsulation of Essential Oils: A Review.

Essential oils (EOs) are complex mixtures of volatile compounds extracted from different parts of plants by different methods. There is a large diversity of these natural substances with varying properties that lead to their common use in several areas. The agrochemical, pharmaceutical, medical, food, and textile industry, as well as cosmetic and hygiene applications are some of the areas where EOs are widely included. To overcome the limitation of EOs being highly volatile and reactive, microencapsulation has become one of the preferred methods to retain and control these compounds. This review explores the techniques for extracting essential oils from aromatic plant matter. Microencapsulation strategies and the available technologies are also reviewed, along with an in-depth overview of the current research and application of microencapsulated EOs.

Non-immersive 3D virtual stimulus alter the time production task performance and increase the EEG theta power in dorsolateral prefrontal cortex.

AIM: The study investigated the cortical activity changes and time production task performance induced by changes in motion speed of a non-immersive 3D virtual stimulus. MATERIAL AND METHODS: Twenty-one individuals were participated in the crossover study with the visual-time reproduction task under three-speed conditions: original, slow and fast virtual stimulus. In addition, the electroencephalographic analysis of the theta band power in the dorsolateral prefrontal cortex was done simultaneously with time production task execution. RESULTS: The results demonstrated that in the slow speed condition, there is an increase in the error in the time production task after virtual reality (p < 0.05). There is also increased EEG theta power in the right dorsolateral prefrontal cortex in all speed conditions (p < 0.05). CONCLUSIONS: We propose that the modulations of speed of virtual stimulus may underlie the accumulation of temporal pulses, which could be responsible for changes in the performance of the production task of the time intervals and a substantial increase in right dorsolateral prefrontal cortex activity related to attention and memory, acting in cognitive domains of supraseconds.

Surface profile of different heat-treated nickel-titanium files before and after root canal preparation

Abstract The aim of this study was to evaluate surface wear, presence of microcracks and surface irregularities of WaveOne (WO) and WaveOne Gold (WOG) instruments before and after multiple uses. Eight Primary instruments of the WO and WOG systems were evaluated, each one was used to prepare six mesial canals of extracted human mandibular molars. The surface of the instruments was evaluated before use (T0), after instrumentation of three (T1) and six (T2) root canals. Surface wear was analyzed using a three-dimensional optical profiler and the presence of microcracks and surface irregularities were evaluated using a tabletop scanning electron microscopy. The Friedman test was used to assess surface wear and Kruskal-Wallis test to evaluate the presence of microcracks and surface irregularities, with a 5% significance level. There was a significant increase in wear in both groups at T2, compared to T0 (p=0.0003). The surface wear after instrumentation of six canals (T2-T0) was statistically greater in the WOG group, than in the WO group (p=0.02), where the presence of microcracks was significantly greater and increased after multiple uses (p<0.05). The presence of surface irregularities in the cutting blade before and after use was statistically greater in the WOG group than WO group (p<0.05). Wear of the cutting blade, microcracks and surface irregularities were observed on the surface topography of all the instruments after multiple uses. These surface changes may affect the cutting efficiency of WOG files and increase the risk of fracture of WO files.

Resumo O objetivo deste estudo foi avaliar o desgaste de superfície, a presença de microtrincas e irregularidades superficiais dos instrumentos WaveOne (WO) e WaveOne Gold (WOG) antes e após múltiplos usos. Oito instrumentos Primary dos sistemas WO e WOG foram avaliados, cada um utilizado no preparo de seis canais mesiais de molares inferiores humanos extraídos. A superfície dos instrumentos foi avaliada antes do uso (T0), após a instrumentação de três (T1) e seis (T2) canais radiculares. O desgaste de superfície foi analisado utilizando um perfilômetro ótico tridimensional e a presença de microtrincas e irregularidades superficiais foram avaliadas usando um microscópio eletrônico de varredura de bancada. O teste de Friedman foi usado para avaliar o desgaste de superfície e o teste de Kruskal-Wallis para avaliar a presença de microtrincas e irregularidades superficiais, com nível de significância de 5%. Houve um aumento significativo no desgaste em ambos os grupos em T2, comparado a T0 (p=0.0003). O desgaste de superfície após a instrumentação de seis canais (T2-T0) foi estatisticamente maior no grupo WOG que no grupo WO (p=0.02), onde a presença de microtrincas foi estatisticamente maior e aumentou após os múltiplos usos (p<0.05). A presença de irregularidades na superfície da lamina de corte antes e após o uso aumento significativamente no grupo WOG comparado ao grupo WO (p<0.05). Desgaste da lâmina de corte, microtrincas e irregularidades superficiais foram observadas na topografia de superfície de todos os instrumentos após os múltiplos usos. Essas alterações na superfície podem afetar a eficiência de corte dos instrumentos WOG e amentar o risco de fratura dos instrumentos WO.

Fires dynamics in the Pantanal: Impacts of anthropogenic activities and climate change.

Anthropogenic activities responsible for modifying climatic regimes and land use and land cover (LULC) have been altering fire behavior even in regions with natural occurrences, such as the Pantanal. This biome was highlighted in 2020 due to the record number of fire foci and burned areas registered. Thus, this study aimed to understand how changes in LULC and climate affect the spatial, temporal and magnitude dynamics of fire foci. The Earth Trends Modeler (ETM) was used to identify trends in spatiotemporal bases of environmental and climatic variables. No trend was identified in the historical series of precipitation data. However, an increasing trend was observed for evapotranspiration, normalized difference vegetation index (NDVI) and temperature. For soil moisture, a decreasing trend was observed. The comparison between the mean of the historical series and the year 2020 showed that the variables precipitation, temperature, soil moisture and evapotranspiration had atypical behavior. Such behavior may have contributed to creating a drier environment with available combustible material, leading to a record number of burned areas, about three million hectares (248%) higher than the historical average. The 2020 fire foci data were used in two types of spatial statistical analyses: Grouping, showing that 76% of the registered fire foci were at high risk of fire and; Hot and Cold Spots, indicating high concentrations of Hot Spots in the northern region of the Pantanal, close to Cerrado and Amazon biomes agricultural frontier. The results of the Land Change Modeler (LCM) tool evidenced a strong transition potential from the natural vegetation to agriculture and pasture in the eastern region of the Pantanal, indicating that this could be, in the future, a region of high concentration of fire foci and possibly high risk of fire. This tool also allowed the prediction of a scenario for 2030 that showed that if measures for environmental protection and combating fires are not adopted, in this year, 20% of the Pantanal areas will be for agricultural and pasture use. Finally, the results suggest that the advance of agriculture in the Pantanal and changes in climatic and environmental variables boosted the increase in fire foci and burned areas in the year 2020.

Shaping efficiency of XP-endo shaper and Mtwo instruments in long oval root canals: A micro-CT study.

This study evaluated the shaping ability of XP-endo Shaper and Mtwo systems in long-oval-shaped canals of extracted human mandibular molars using micro-computed tomography (micro-CT). Images recorded before and after preparation were evaluated for morphometric measures regarding increase in canal volume, dentin removed, surface area, untouched walls and structure model index (SMI). Data were statistically analysed using the Mann-Whitney test with 5% of significance. On both groups, the root canal preparation significantly increased all analysed parameters (P > 0.05). There was no statistical difference between XP-endo Shaper and Mtwo systems in the increase of the canal volume (30.50%-27.82%), in volume of dentin removed (2.77%-2.40%), in the increase of the canal surface area (11.30%-8.86%), in canal untouched surfaces (9.57%-8.51%) and in the SMI (2.59%-2.68%), respectively. XP-endo Shaper and Mtwo systems showed similar shaping efficiency and were not able to completely prepare the walls of long-oval-shaped canals of extracted human mandibular molars.

Peritoneopericardial diaphragmatic hernia in a healthy adult feline (Felis catus domesticus): diagnosis to surgical treatment - Case report.

Peritoneopericardial diaphragmatic hernia (PPDH) is a communication between the abdomen and the pericardial sac generated by congenital anomalies triggered during diaphragmatic and pericardial development. This report aimed to present the case of an adult, mixed-breed cat, affected by PPDH, focusing on the period from diagnosis to successful surgical correction. The patient had a capricious appetite and weight loss for about four months and started, at the end of this period, a state of apathy. On abdominal ultrasound, the gallbladder (GB) was close to the heart, suggesting diaphragmatic discontinuity. On thoracic radiography, there were changes suggestive of PPDH, pericardial efusion or cardiomegaly with probable dilated cardiomyopathy. Based on these findings, an echocardiogram was performed, highlighting the hepatic lobe and GB internally to the pericardium, causing cardiac compression, although without severe cardiac changes. During surgery, a diaphragmatic defect of 4 cm in diameter was observed with the congested right medial hepatic lobe and hyperemic GB in the pericardial sac. The defect was sutured using the sultan pattern in separate stitches and polyamide threads. The feline returned to feeding with greater interest soon after the surgery, and after 15 days it was fed with dry food and had normal behavior. PPDH can be diagnosed in healthy adult cats, even if there are no apparent respiratory, gastrointestinal, or cardiac signs. The echocardiogram is relevant in the definitive diagnosis, in addition to excluding differential diagnoses, and simple surgical treatment with polyamide thread and sultan suture is successful.

A hérnia periotônio-pericárdica diafragmática (HPPD) comunica o abdome e o saco pericárdico, é gerada por anomalias congênitas deflagradas no desenvolvimento diafragmático e pericárdico. O objetivo deste relato é apresentar o caso de uma gata adulta, mestiça, acometida por HPPD, destacando do diagnóstico à correção cirúrgica bem sucedida. A paciente exibia apetite caprichoso e emagrecimento há cerca de 4 meses, iniciando ao final deste período, apatia. Na ultrassonografia abdominal a vesícula biliar (VB) estava próxima ao coração sugerindo ruptura diafragmática. Na radiografia torácica evidenciou-se alterações sugestivas de HPPD ou cardiomegalia com provável miocardiopatia dilatada. Devido tais achados realizou-se ecocardiograma destacando o lobo hepático e a VB no pericárdio comprimindo o coração sem comprometer sua função. Na cirurgia observou-se defeito diafragmático (4 cm), passagem do lobo hepático medial direito e da VB para o pericárdio. Suturou-se o defeito com padrão sultan e fio poliamida 3.0. A felina voltou a se alimentar com maior interesse logo após a cirurgia. A HPPD pode ser diagnosticada em felinos adultos saudáveis, mesmo que não haja sinais respiratórios, gastrointestinais ou cardíacos aparentes. O ecocardiograma é relevante no diagnóstico definitivo, além de excluir diagnósticos diferenciais, sendo o simples tratamento cirúrgico com fio poliamida e sutura sultan bem sucedido.

Surface profile of different heat-treated nickel-titanium files before and after root canal preparation.

The aim of this study was to evaluate surface wear, presence of microcracks and surface irregularities of WaveOne (WO) and WaveOne Gold (WOG) instruments before and after multiple uses. Eight Primary instruments of the WO and WOG systems were evaluated, each one was used to prepare six mesial canals of extracted human mandibular molars. The surface of the instruments was evaluated before use (T0), after instrumentation of three (T1) and six (T2) root canals. Surface wear was analyzed using a three-dimensional optical profiler and the presence of microcracks and surface irregularities were evaluated using a tabletop scanning electron microscopy. The Friedman test was used to assess surface wear and Kruskal-Wallis test to evaluate the presence of microcracks and surface irregularities, with a 5% significance level. There was a significant increase in wear in both groups at T2, compared to T0 (p=0.0003). The surface wear after instrumentation of six canals (T2-T0) was statistically greater in the WOG group, than in the WO group (p=0.02), where the presence of microcracks was significantly greater and increased after multiple uses (p<0.05). The presence of surface irregularities in the cutting blade before and after use was statistically greater in the WOG group than WO group (p<0.05). Wear of the cutting blade, microcracks and surface irregularities were observed on the surface topography of all the instruments after multiple uses. These surface changes may affect the cutting efficiency of WOG files and increase the risk of fracture of WO files.

Additional Improvement of Respiratory Technique on Vascular Function in Hypertensive Postmenopausal Women Following Yoga or Stretching Video Classes: The YOGINI Study.

Background: Hypertension remains highly prevalent in postmenopausal women, along with vascular dysfunction and increased oxidative stress. In such context, regular exercises, yoga practice, and slow breathing have been recommended to treat hypertension. However, the effects of the multiple components of yoga, including the respiratory techniques involved in the practice, on hypertension and on vascular and endothelial function have never been evaluated. Objective: This study aimed to investigate the additional effects of respiratory technique on vascular function and oxidative stress profile in hypertensive postmenopausal women (HPMWs) following yoga or stretching video classes. Study Design: Hypertensive postmenopausal women were recruited and randomized for 12 weeks, twice a week, of supervised yoga or stretching video classes of 75 min for 12 weeks associated or not with respiratory technique. Baseline and post-intervention measurements included pulse wave velocity (PWV), flow-mediated dilation (FMD), and oxidative stress parameters. Hypertensive postmenopausal women (59 ± 0.7 years) who ended the protocol were distributed into three groups: (1) control group (yoga or stretching, C, n = 14); (2) yoga + respiratory technique (Y+, n = 10); (3) stretching + respiratory technique (S+, n = 9). Results: Diastolic blood pressure and FMD [baseline: C: 6.94 ± 1.97%, Y+: 7.05 ± 1.65%, and S+: 3.54 ± 2.01% vs. post: C: 16.59 ± 3.46% (p = 0.006), Y+: 13.72 ± 2.81% (p = 0.005), and S+: 11.79 ± 0.99% (p = 0.0001)] have significantly increased in all groups when baseline and post-practice values were compared. However, resting heart rate and PWV [baseline: Y+: 10.44 ± 3.69 and S+: 9.50 ± 0.53 m/s vs. post: Y+: 9.45 ± 0.39 (p = 0.003) and S+: 8.02 ± 0.47 m/s (p = 0.003)] decreased significantly only in the Y+ and S+ groups (baseline vs. post). Systemic antioxidant enzyme activities (superoxide dismutase and catalase) increased in all groups, and hydrogen peroxide and lipoperoxidation reduced in Y+ and S+ (baseline vs. post). Conclusions: Twelve weeks of yoga or stretching video classes promoted positive changes in several outcomes generally regarded as cardiovascular risk factors in HPMWs, and these changes were even more pronounced by the association with respiratory technique.

Effectiveness of the automated drug dispensing system: systematic review and meta-analysis.

OBJECTIVES: to compare the effectiveness of the decentralized automated drug dispensing system with pockets. METHODS: an effectiveness study based on a systematic review guided by the question: for patients admitted to hospital units, is the use of automated drug dispensing effective for reducing medication errors when compared to manual unit dose dispensing? The evidence was evaluated by the Grading of Recommendations Assessment, Development and Evaluation and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses instrument, used in the report. RESULTS: the sample was composed of 15 studies and none of them directly compared both technologies; however, the meta-analysis showed that there is no difference in effectiveness between them [OR 1.03 95%CI (0,12 - 8,99)]. CONCLUSIONS: the conclusion is that the recommendation in favor of the automated dispensing system is weak.

Inhibition of Na+, K+ -ATPase with ouabain is detrimental to equine blastocysts.

Although equine blastocysts ≤ 300 µm in diameter can be successfully vitrified, larger equine blastocysts are not good candidates for cryopreservation. As Na+, K+-ATPase is involved in maintaining blastocyst expansion, perhaps inhibition of this enzyme would be a viable method of reducing blastocyst diameter prior to cryopreservation. Objectives were to evaluate effects of ouabain-induced inhibition of Na+, K+-ATPase in equine blastocysts. Sixteen mares were ultrasonographically monitored, given deslorelin acetate to induce ovulation, and inseminated. Embryos (D7 and D9) were harvested and Na+, K+-ATPase inhibited for 1 or 6 h by exposure to 10-6 M ouabain, either natural ouabain or conjugated to fluorescein (OuabainFL), during incubation at 37° C. Evaluations included morphometric characteristics (bright field microscopy) and viability (Hoescht 33342 + propidium iodide). Blastocysts incubated for 6 h in Holding medium + ouabain (n=3) had, on average, a 45.7% reduction in diameter, with adverse morphologic features and no re-expansion after subsequent incubation in Holding medium for 12 h. In subsequent studies, even a 1-h exposure to Ouabain or OuabainFL, caused similar reductions, namely 38.7 ± 6.7% (n=5) and 33.6 ± 3.3% (n=7) for D7 and D9 blastocysts, respectively. Ouabain binding was confirmed after OuabainFL exposition and all embryos (n=12) lost viability. We concluded that Na+, K+-ATPase inhibition with ouabain caused death of equine blastocysts and therefore was not a viable method of reducing blastocyst size prior to cryopreservation.