A high salt intake in early life affects stress-coping response in males but not in female rats.

Eating diets high in salt has been associated with alterations in the immune system and the potential development of neuropsychiatric disorders. This area of research shows promise, but there is currently a limited amount of research on this topic. The present study investigated whether a high salt diet (HSD) affects anhedonia and stress-coping response behaviors in young male and female Wistar rats. In this study, male and female Wistar rats were fed an HSD (8 % NaCl w/w) from weaning to post-natal day (PND) 64. From PND 60 to 64, the rats underwent a spontaneous locomotor activity test (SLA), sucrose splash test (SST), sucrose preference test (SPT), and forced swim test (FST), followed by euthanasia at PND 65. Male and female rats consuming the HSD exhibited an increase in water intake compared to the corresponding control diet (CD) groups. Male rats had lower body weight despite having similar food intakes compared to the CD group. Male rats displayed an active stress-coping behavior in the FST, characterized by increased mobility. Additionally, HSD-fed males exhibited a greater preference for sucrose solution in the SPT. However, no effect of diet and sex were detected in the SST and the SLA, and hypothalamic levels of leptin and ghrelin receptors. On the other hand, female rats were less susceptible to the experimental conditions applied in this protocol than males.

Perceived impact of dental pain on the quality of life of children aged 1-3 years and their families.

PURPOSE: To evaluate the perceived impact of dental pain on oral health-related quality of life (OHRQoL) among children aged 1-3 years and their families. METHODS: A cross-sectional study was conducted with 306 randomly selected preschool and day care children in the city of Diamantina, Brazil. Parents/caregivers answered a questionnaire on socio-demographic information, the Brazilian version of the Early Childhood Oral Health Impact Scale, their child's general/oral health and the Brazilian version of the Dental Discomfort Questionnaire. The children underwent an oral examination. Descriptive statistics, Spearman's correlations, the Kruskal-Wallis test, Mann-Whitney test and Poisson regression were used to determine associations between the variables (prevalence ratio [PR], α = 5%). RESULTS: The prevalence of dental caries and dental pain was 43.1% and 40.2%, respectively. Dental pain (PR: 1.96, 95% CI 1.52-2.54; p < 0.001), monthly household income (PR: 1.38, 95% CI 1.04-1.83; p = 0.026) and dental caries (PR: 1.06, 95% CI 1.03-1.10; p < 0.001) were significantly associated with the perceived impact on OHRQoL. CONCLUSIONS: Dental pain, household income and dental caries were associated with a negative impact on the OHRQoL of children aged 1-3 years and their families.

Association between malocclusion and dental caries in adolescents: a systematic review and meta-analysis.

AIM: To evaluate the scientific evidence regarding the association between malocclusion and dental caries in adolescents. METHODS: Searches were conducted of six electronic databases, complemented by manual searching of the reference lists of the selected articles and grey literature. Two independent reviewers performed the selection of the articles, data extraction and the evaluation of the risks of bias through an assessment of methodological quality. Meta-analysis was performed considering the mean decayed, missing and filled teeth (DMFT) index for caries and the Dental Aesthetic Index (DAI) for malocclusion. Heterogeneity was tested using the I2 statistic and a random effect model was employed. Summary effect measures were calculated as differences in means. RESULTS: The initial search retrieved 2644 studies, only 15 of which were selected for full-text analysis. Four cross-sectional studies were included in the qualitative systematic review. Only one of these studies found no association between malocclusion and dental caries. The meta-analysis of three studies demonstrated that a lower DAI value was significantly associated with a lower mean DMFT index, except for the comparison of DAI 26-30 vs. 31-35. CONCLUSIONS: Based on the studies analysed, the scientific evidence indicates an association between malocclusion and dental caries.

Gene Expression of Monocarboxylate Transporters and Oocyte-secreted Factors in Bovine Cumulus-oocyte Complexes Selected by Brilliant Cresyl Blue.

Oocyte selection based on the brilliant cresyl blue (BCB) staining test has been successfully used to differentiate between competent and incompetent bovine oocytes. Here, the expression of genes involved in transport of monocarboxylates (Mct1-4) and oogenesis specific genes (Bmp15, Gdf9 and Has2) in BCB+ and BCB- selected immature and mature bovine cumulus-oocyte complexes (COC) was evaluated. In order to find specific molecular markers to characterize successful oocyte maturation, our study was also aimed at identifying the expression of Mcts and oogenesis specific genes in denuded oocytes and cumulus cells. Immature COCs morphological appropriate were (i) stained with 26 mm BCB for 90 min before IVM, (ii) exposed to same incubation conditions as stained COCs, but without BCB (holding group) or (iii) transferred into a maturation medium immediately after morphological selection (control group). mRNA expression was investigated by RT-PCR in COCs before and after IVM. No relationship was observed in the relative expression of Has2, Gdf9, Bmp15 or Mct1, 2 and 4 transcripts between BCB+ and BCB- COCs. Transcripts analysis showed that Gdf9 and Bmp15 in BCB+, BCB- and holding groups were up-regulated (p < 0.05) before IVM, while Has2 was up-regulated (p < 0.01) after IVM in the control group. Other genes remained stable during maturation (Mct1, 2 and 4). Our results showed, for the first time, Mct1, 2 and 4 expression in bovine COCs. Mct1 and Mct4 transcripts were present in denuded oocytes and cumulus cell, while Mct2 was detected only in cumulus cells. These differences between the three isoforms in localization suggest unique roles for each in monocarboxylate transport during maturation.

Malocclusion in preschool children: prevalence and determinant factors.

PURPOSE: To evaluate the prevalence of malocclusion and associated factors in the primary dentition of preschoolers in the city of Diamantina, Brazil. METHODS: A cross-sectional study was carried out with 381 children aged 3-5 years treated at the 10 basic health care units in the city during immunisation campaigns. The dependent variables (presence of malocclusion, open bite, crossbite and crowding) were evaluated through a clinical oral exam. The independent variables (gender, age, health problems, breastfeeding, bottle feeding, harmful oral habits, mother's schooling, household income and number of children in the home) were collected through interviews. Statistical analysis involved descriptive analysis, Chi square test and Poisson regression. RESULTS: The prevalence of malocclusion was 32.5%. Open bite was the most frequent type of malocclusion. Children with a history of bottle feeding (PR 1.74; 95% CI 1.24-2.44) and those with harmful oral habits (PR 1.49; 95% CI 1.23-1.99) had greater prevalence rates of malocclusion. Greater prevalence rates of open bite were also found in children with a history of bottle feeding (PR 5.00; 95% CI 1.99-12.5) and those with harmful oral habits (PR 2.90; 95% CI 1.59-5.29), whereas greater prevalence rates of crossbite were found in boys (PR 1.79; 95% CI 1.10-2.91) and were associated with mother's schooling (PR 1.91; 95% CI 1.20-3.06). CONCLUSION: A history of bottle feeding and the presence of harmful oral habits were identified as determinants for the occurrence of malocclusion in preschoolers.

Evaluation of prenucleic acid extraction for increasing sensitivity of detection of virus in bovine follicular fluid pools.

Bovine viral diarrhea virus (BVDV), bovine herpesvirus type 1 (BoHV-1), and bovine herpesvirus type 5 (BoHV-5) are major cattle pathogens that can be present in biological materials used in assisted reproduction biotechnologies. The aim of the present study was to increase the sensitivity of the polymerase chain reaction (PCR) for detection of BVDV, BoHV-1, and BoHV-5 in bovine follicular fluid (FF) collected during oocyte retrieval for in vitro embryo production. Ovaries were collected immediately after slaughter at a commercial abattoir, aspirated, and the 7336 samples of FF were pooled in 84 samples. Before testing the FF field samples, sensitivity of the protocol was determined using a prenucleic acid extraction procedure that was directly compared with standard RNA or DNA extraction protocols. The prenucleic acid extraction procedure increased sensitivity of reverse transcription (RT)-PCR for BVDV and nested PCR for BoHV-1 and BoHV-5 by 100 and 10 times, respectively. The 84 FF pools were assayed for BVDV, BoHV-1, and BoHV-5 using virus isolation and RT-PCR or nested PCR. Fourteen (16.7%) FF pools were positive for BVDV RNA, and one (1.2%) was positive for BoHV-1 DNA. Two of the BVDV RT-PCR positive samples and the one BoHV-1 PCR positive sample were also positive in cell culture, demonstrating that FF contained infectious viruses. In this study, the prenucleic acid extraction procedure increased the sensitivity of RT-PCR and PCR detection. This study highlighted the importance of assuring biosecurity by detecting the presence of viral pathogens in biological materials used during in vitro embryo production.

Impact of early childhood caries on the oral health-related quality of life of preschool children and their parents.

The aim of the present population study was to evaluate the impact of early childhood caries (ECC) on the oral health-related quality of life (OHRQoL) of preschool children and their parents/caregivers. A random sample of 638 children (aged 2-5 years) underwent a clinical oral examination to assess ECC, and their parents were invited to answer two questionnaires: one on the OHRQoL of the child, the Early Childhood Oral Health Impact Scale, and another on the characteristics and sociodemographic conditions of the child. Descriptive analysis, χ(2) test, Mann-Whitney test, Kruskal-Wallis test, and hierarchically adjusted Poisson regression models were used. The prevalence of ECC was 52.2%. The number of teeth with decay ranged from 1 (n = 42; 6.6%) to 20 (n = 5; 0.8%), averaging 2.86 (SD = 4.04). There was a significant difference between the severity of ECC and OHRQoL in terms of the impact on both child and family (p < 0.001). An increase in the severity of ECC resulted in an increased negative impact on the quality of life of the child (rate ratio, RR = 5.32; 95% confidence interval, CI: 3.67-7.71). Greater age of the mother had a positive impact on the OHRQoL of preschool children (RR = 0.72; 95% CI: 0.54-0.97). Increased age resulted in an increased negative impact on the quality of life of the child (RR = 2.97; 95% CI: 1.61-5.47). ECC has a negative impact on the OHRQoL of children aged 2-5 years and their parents. Mothers aged 30 or older reported better OHRQoL, independent of the presence of ECC and the age of the child.

Masticatory performance and taste perception in patients submitted to cancer treatment.

The aim of this study was to analyse mastication and the sense of taste in 39 patients submitted to cancer treatment in different areas of the body and to compare these variables with those of 44 control individuals within the same age range. The following aspects were assessed: dental status (DMFT); stimulated and non-stimulated salivary flow; sense of taste (salty, sweet, bitter and sour); and masticatory performance (MP), through the calculation of X(50). Logistic regression models were established to test the association between the independent variables and cancer treatment. Cancer patients had lesser stimulated salivary flow, a smaller number of teeth and occlusal units, worse MP, higher salty, sweet and sour taste scores and a lower bitter taste score (P < 0·05). A significant positive correlation was found between MP and the DMFT index in both groups (P < 0·05), meaning that a lower DMFT index value denoted a smaller X(50) value (better masticatory performance). The logistic regression model revealed that patients who had undergone cancer treatment had a greater probability of exhibiting a smaller number of teeth, higher salty and smaller bitter taste scores (P < 0·05). It was concluded that patients who were submitted to cancer treatment presented oral physiology alterations when compared with control subjects at the same age range.

Untreated dental caries: impact on quality of life of children of low socioeconomic status.

PURPOSE: This study's purpose was to evaluate the impact of untreated dental caries on the oral health-related quality of life (OHRQoL) of children of low socioeconomic status. METHODS: One hundred twelve impoverished 8- to 10-year-olds from a city in southeastern Brazil were selected. OHRQoL was measured using the Child Perceptions Questionnaire (CPQ8-10; total and subscale scores) and its global question on oral health. The clinical examination recorded the prevalence of untreated dental caries (corresponding to a non-zero D or d component in the DMFT or dmft indices). The children were categorized as without untreated dental caries=0 or with untreated dental caries ≥1. Malocclusions and dental trauma were determined using the Dental Aesthetic Index and Andreasen and Andreasen (1994) classification, respectively. Descriptive analysis, Mann-Whitney, chi-square test, and hierarchically adjusted Poisson regression models were employed. RESULTS: Eighty-four children (75%) had 1 or more carious teeth. The relative risk of having a negative perception of oral health was greater among the children with untreated dental caries (RR-1:41; 95% confidence interval=1.13-1.75), regardless of gender or malocclusion. CONCLUSION: Eight- to 10-year-olds with untreated dental caries have a greater relative risk of having a negative perception of oral health status than those without dental caries.

Malocclusion: social, functional and emotional influence on children.

OBJECTIVES: To determine the association between types of malocclusion and quality of life in children between 8-10 years of age and establish correlations between the severity of the malocclusion and particular bio-psychosocial variables. STUDY DESIGN: The sample was made up of 102 schoolchildren aged 8-10 years. Clinical exams were performed using the criteria of the Dental Aesthetic Index (DAI) to determine the presence and severity of malocclusions. The impact on quality of life was assessed using the Child Perceptions Questionnaire (CPQ8-10). Statistical analysis involved the chi-square test, Fisher's exact test and Spearman's correlation analysis. RESULTS: Malocclusions affected 61% of the children examined. There was a positive correlation between total CPQ8-10 and DAI scores (P = 0.034). The following types of malocclusion had a significant effect on the quality of life of the children: upper anterior irregularity > or = 2 mm, anterior open bite > or = 2 mm and diastema > or = 2 mm. Children with malocclusion experienced a greater negative impact on quality of life in comparison to those without malocclusion. CONCLUSIONS: Malocclusions had a negative influence over the quality of life of children between 8-10 years of age. More severe malocclusions had a greater impact with regard to social, emotional and functional aspects.

Palatal radicular groove: clinical implications of early diagnosis and surgical sealing.

Palatal radicular groove is a discreet alteration in tooth morphology, characterized by an invagination that begins near the cingulum of the tooth and moves in an apical direction. Clinically, palatal radicular groove may be associated with periodontal and/or endodontic problems. This paper describes a clinical case of a young patient with palatal radicular groove with no signs of periodontal disease or endodontic impairment. An early diagnosis was made and treatment consisted of surgical sealing of the defect. After a 2-year period, reexaminations demonstrated adequate hygiene, maintenance of tooth vitality and periodontal health. The early diagnosis and sealing of the groove observed surgically made the root surface smooth, avoiding subgingival bacterial plaque buildup, and preventing possible periodontal and/or pulp impairment stemming from the defect.

Avaliação da adequação legal de receitas e notificações de receita de medicamentos sujeitos a controle especial dos setores públicos e privados / Evaluation of the legal prescription and notifications adequacy for special control medicinesin the public and private sectors

Os objetivos desta pesquisa foram avaliar a adequação legal de receitas de controle especial e notificações de receitas dispensadas em uma Drogaria de Varginha ? MG, estabelecer uma comparação entre as prescrições provenientes da rede pública e privada e detectar as classes de medicamentos mais prescritas. Analisaram-se 960 prescrições através de um formulário com itens obrigatórios de acordo com a legislação vigente. Os dados obtidos foram analisados pelo Teste Qui-quadrado, utilizando a origem das notificações e receitas como desfecho. Considerando as receitas; a única variável que apresentou diferença entre os setores público e privado foi a descrição por extenso da quantidade de medicamento, que foi mais adequadamente cumprida pelo setor privado. Em relação às notificações, a legibilidade foi significativamente mais freqüente em notificações provenientes de centros públicos, enquanto a designação da Unidade Federativa e a descrição por extenso da quantidade de medicamento foram mais presentes no setor privado. Por outro lado, a descrição completa da posologia, da data da notificação, a presença de carimbo e descrição da forma farmacêutica foram mais adequadas no setor público. Nenhuma das prescrições foi considerada totalmente adequada aos dados exigidos pela Portaria 344/98. As classes medicamentosas mais prescritas foram os benzodiazepínicos em notificações de receita e os antidepressivos em receituário de controle especial. Concluiu-se que a prescrição de medicamentos sujeitos a controle especial apresenta falhas de acordo com a legislação vigente.

The objectives of the present study were to evaluate the legal prescription adequacy in one drugstore located in the south of Minas Gerais state, in order to establish comparisons between the prescriptions of public and private services and to detect the most commonly classes of medicines prescribed. It was analyzed 960 prescriptions in accordance with the current law. The data were analyzed by descriptive statistics and compared using frequency distribution test(Chi-square test) considering private and public sources of prescription as the dependent variable. Considering special control prescriptions, the extensive description of the amount of medicine was fulfilled more frequently by the private sector. However, for the notifications; legibility, dosage, pharmaceutical form, date of issue and presence of stamp were more frequently present in the public service, while assignment of the Federative Union, the extensive description of the amount of medicine and the dosage was more present in the private sector. None of the evaluated prescriptions was considered totally adjusted with law 344/98. The prescribed medicines more commonly found were benzodiazepines for prescription notifications and antidepressants in special control prescriptions. It was concluded that the medicine prescriptions subjected to special control are frequently not adequate to the current law.

Impact of exposure to positive images on dental anxiety among children: a controlled trial.

AIM: To determine whether exposing children to images of positive dental care would have an effect on their degree of anxiety, assessing anxiety three separate times. STUDY DESIGN: Controlled trial. METHODS: The 70 participants from 4-11 years of age were randomly assigned to one of two conditions. The intervention consisted of viewing positive images of dentistry and dental treatment (n=35). The control condition consisted of dentally neutral images (n=35). OUTCOME MEASURES: Anxiety was assessed using the Venham Picture Test (VPT) prior to the intervention, immediately following the intervention and following the dental appointment. STATISTICS: Statistical analysis (chisquare and Mann-Whitney tests) was conducted blind to group allocation. RESULTS: No significant difference was detected between the scores of the VPT in the two groups at any evaluation time (p>0.05). Dental anxiety at the three evaluation times was not correlated to age. There was no difference in level of anxiety between male and female participants (p>0.05). CONCLUSIONS: Viewing positive images of dentistry and dentists did not have a greater effect on child anxiety in the dental setting than viewing neutral images, however, showed lower rates of anxiety for all children although this was not significant.

Prevalence and risk indicators of temporomandibular disorder signs and symptoms in a pediatric population with spastic cerebral palsy.

OBJECTIVE: To determine risk indicators for signs and symptoms of temporomandibular disorders (TMD) in children with cerebral palsy (n = 60) and control subjects (n = 60). STUDY DESIGN: The subjects were assessed by means of questionnaire and clinical exam: 1) signs and symptoms of TMD; 2) malocclusions [Dental Aesthetic Index (DAI)]; 3) harmful habits; and 4) bio-psychosocial characteristics. Statistical analysis involved the chi-square, Fisher's exact tests (p < or = 0.05) and multivariate logistic regression (forward stepwise procedure). Variables that achieved a p-value < or = 0.20 were used as potential predictors of signs and symptoms of TMD and applied as co-variables in the multivariate analysis. RESULTS: The prevalence of at least one sign and/or symptom of TMD in the present sample was 1.7% (n = 1) among the individuals in the control group and 13.3% (n = 8) among the individuals with cerebral palsy. The presence of cerebral palsy (Odds Ratio: 9.08; p = 0.041), male gender (OR: 6.21; p = 0.027), severity of the malocclusion (OR: 4.75; p = 0.031), mouth breathing (OR: 5.40; p = 0.022) and mixed dentition (OR: 4.73; p = 0.035) were identified as risk indicators for signs and symptoms of TMD. CONCLUSIONS: It was concluded that children with cerebral palsy had a significantly greater chance of developing signs and symptoms of TMD.

Self-perception regarding the need for orthodontic treatment among impoverished schoolchildren in Brazil.

AIM: The objectives of the present study were to assess predictive factors associated to self-perception regarding the need for orthodontic treatment among 170 impoverished schoolchildren. MATERIALS AND METHODS: A cross- sectional study was conducted in public schools in the city of Belo Horizonte-MG (Brazil). Children's self-perception regarding the need for treatment was assessed using the Aesthetic Component of the Index of Orthodontic Treatment Need (IOTN-AC) and a questionnaire addressing biopsychosocial factors. The chi-square test and multiple logistic regression were used in the data analysis. RESULTS: The predictive factors for the self-perception of orthodontic treatment need on the part of the impoverished schoolchildren included ridicule from persons close to the children regarding the appearance of the teeth (OR 2.81; 95%CI 1.2 to 6.4), dissatisfaction with oral aesthetics due to the alignment of the teeth (OR 2.45; 95%CI 1.1 to 5.4) and missing teeth (OR 3.02; 95%CI 1.0 to 9.23). CONCLUSION: The alignment of the teeth, missing teeth and ridicule from others regarding the appearance of the teeth are the factors that most strongly influence self- perception regarding the need for orthodontic treatment among impoverished schoolchildren.

Predictive factors for child behaviour in the dental environment.

AIM: This was to evaluate the potential factors that influence behaviour in the paediatric dentistry environment of children aged 48 to 68 months who have never before been to a dentist. STUDY DESIGN: Cross sectional. METHODS: Child behaviour was evaluated using the Frankl scale during both clinical examination procedures and prophylaxis. All mothers of the 118 children investigated underwent a test to assess their anxiety (Manifest Anxiety Scale-MAS) and responded to a questionnaire on the medical history of the child as well as socioeconomic factors. The children were given a test to assess their anxiety (Venham Picture Test-VPT). STATISTICS: The statistical tests adopted were the Chi-squared test and both simple and multiple unconditional logistic regression. RESULTS: Children with high anxiety, those had previously experienced toothache and those had behaved uncooperatively during prior medical experiences, had a greater probability of behaving uncooperatively during their first dental consultation. CONCLUSIONS: Dental professionals can identify the presence of predictive factors for child behaviour prior to the first clinical examination by including relevant questions in the dental and medical history, thereby facilitating a more positive initial contact between paediatric dentists and their patients.

Determination of thorium concentrations and activity ratios in silicate rocks by alpha spectrometry.

A detailed radiochemical procedure for alpha spectrometry measurements of thorium concentrations and of 230Th/232Th activity ratios in silicates is presented. The Th behaviour, during each step of the chemical process, was investigated by using a 234Th tracer, which is a gamma-ray emitter. The described chemical processing provides relatively high thorium yields, which varied between 56% and 88%, in the analysis of GB-1 (granite) and BB-1 (basalt) Brazilian geological standards. Also, the application of the established radiochemical method allowed a determination of both Th concentrations and activity ratios with high reproducibility, on the order of 2%. The estimation of the concentration result accuracy is also about 2%, which was calculated by using published data obtained from neutron activation analysis as reference values.

Determination of uranium concentrations and activity ratios in silicates by alpha spectrometry: application to the volcanic rocks from the Trindade and Martin Vaz Islands (Brazil).

A detailed radiochemical procedure for alpha spectrometry measurements of uranium concentrations and of 234U/238U activity ratios in silicates is presented. The described chemical processing is characterized by relatively high uranium yields, in the range of 51-89%. The application of this chemical procedure to geological standards allowed estimating a reproducibility of the alpha spectrometry uranium measurements on the order of 3.5%. As an application example. the procedure was used in the analysis of quaternary volcanic rocks from the Trindade and Martin Vaz Islands, Brazil.

Investigacao da variabilidade da frequencia cardiaca de mulheres nos periodos manha e noite / Investigation of heart rate variability in women during morning and evening periods

A proposta desta investigacao foi comparar a variabilidade da frequencia cardiaca (VFC) de mulheres de manha e a noite. Foram estudadas sete mulheres saudaveis, com idade media de 23(+-2, 03) anos. As voluntarias foram avaliadas em repouso nas posicoes supina e sentada e durante o teste de esforco fisico dinamico do tipo degraus descontinuos, submaximo, nas potencias de 20, 30, 40 e 50 watts(W) em cicloergometro eletromagnetico na posicao sentada. A frequencia cardiaca foi coletada a partir do eletrocardiograma em tempo real pela manha (8h) e pela noite (20h). A VFC foi analisada pelo indice de RMSSD dos intervalos R-R(iR-R) em milssegundos (ms). Em relacao aos periodos do dia, foi observado que a VFC no periodo da manha, em comparacao ao periodo da noite, nas condicoes de repouso supino, sentado e durante o exercicio fisico na potencia de 40 W apresentou diferenca estatisticamente significativa (p< 0,05). Os resultados mostram ainda, que da transicao do repouso para o exercicio fisico nos dois periodos ocorre uma diminuicao crescente da VFC com o aumento dos niveis de potencia. Esses resultados sugerem maior modulacao vagal no periodo da manha, tanto no repouso quanto no exercicio fisico