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1.
Endocrinology ; 152(11): 4046-52, 2011 Nov.
Article in English | MEDLINE | ID: mdl-21914773

ABSTRACT

The maintenance of appropriate glucose levels is necessary for survival. Within the brain, specialized neurons detect glucose fluctuations and alter their electrical activity. These glucose-sensing cells include hypothalamic arcuate nucleus neurons expressing neuropeptide Y (NPY) and lateral hypothalamic area (LHA) neurons expressing orexin/hypocretins (ORX) or melanin-concentrating hormone (MCH). Within the LHA, a population of NPY-expressing cells exists; however, their ability to monitor energy status is unknown. We investigated whether NPY neurons located in the LHA, a classic hunger center, detect and respond to fluctuations in glucose availability and compared these responses with those of known LHA glucose sensors expressing ORX or MCH. Using mice expressing green fluorescent protein under the control of NPY regulatory elements, we identified LHA NPY cells and explored their anatomical distribution, neurochemical and electrical properties, in vivo responses to fasting and insulin-induced hypoglycemia, and in situ electrical responses to extracellular glucose. We report that NPY, ORX, and MCH are expressed in nonoverlapping populations within the LHA. Subpopulations of LHA NPY neurons were activated in vivo by both a 6-h fast and insulin-induced hypoglycemia. Likewise, increased extracellular glucose suppressed the electrical activity of approximately 70% of LHA NPY neurons in situ, eliciting hyperpolarization and activating background K+ currents. Furthermore, we report that the glucose sensitivity of LHA NPY neurons is significantly different from neighboring ORX and MCH neurons. These data suggest that NPY-expressing cells in the LHA are a novel population of glucose-sensing neurons that represent a new player in the brain circuitry integrating information about glucose homeostasis.


Subject(s)
Glucose/metabolism , Hypothalamic Area, Lateral/metabolism , Neurons/metabolism , Neuropeptide Y/metabolism , Animals , Female , Food Deprivation/physiology , Glucose/pharmacology , Hypoglycemia/chemically induced , Hypoglycemia/metabolism , Hypothalamic Area, Lateral/drug effects , Hypothalamic Hormones/metabolism , Intracellular Signaling Peptides and Proteins/metabolism , Male , Melanins/metabolism , Mice , Mice, Transgenic , Neurons/drug effects , Neuropeptides/metabolism , Orexins , Pituitary Hormones/metabolism
2.
Cell Metab ; 13(5): 584-91, 2011 May 04.
Article in English | MEDLINE | ID: mdl-21531340

ABSTRACT

Serotonin (5-HT) and leptin play important roles in the modulation of energy balance. Here we investigated mechanisms by which leptin might interact with CNS 5-HT pathways to influence appetite. Although some leptin receptor (LepRb) neurons lie close to 5-HT neurons in the dorsal raphe (DR), 5-HT neurons do not express LepRb. Indeed, while leptin hyperpolarizes some non-5-HT DR neurons, leptin does not alter the activity of DR 5-HT neurons. Furthermore, 5-HT depletion does not impair the anorectic effects of leptin. The serotonin transporter-cre allele (Sert(cre)) is expressed in 5-HT (and developmentally in some non-5-HT) neurons. While Sert(cre) promotes LepRb excision in a few LepRb neurons in the hypothalamus, it is not active in DR LepRb neurons, and neuron-specific Sert(cre)-mediated LepRb inactivation in mice does not alter body weight or adiposity. Thus, leptin does not directly influence 5-HT neurons and does not meaningfully modulate important appetite-related determinants via 5-HT neuron function.


Subject(s)
Appetite , Brain/drug effects , Leptin/pharmacology , Neurons/drug effects , Receptors, Leptin/physiology , Serotonin Plasma Membrane Transport Proteins/physiology , Serotonin/metabolism , Animals , Body Weight/drug effects , Brain/cytology , Brain/metabolism , Electrophysiology , Hypothalamus/cytology , Hypothalamus/drug effects , Hypothalamus/metabolism , Immunoenzyme Techniques , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Neural Pathways , Neurons/cytology , Neurons/metabolism , Obesity/etiology , Obesity/metabolism
3.
Eur J Pharmacol ; 660(1): 70-9, 2011 Jun 11.
Article in English | MEDLINE | ID: mdl-21216242

ABSTRACT

Body weight homeostasis is critically dependent upon the convergence and integration of multiple central and peripheral signalling systems that collectively function to detect and elicit physiological and behavioural responses to nutritional state. To date, only a minority of these signals have been pharmacologically targeted for the treatment of human obesity. One signal that has been effectively manipulated to reduce body weight is the neurotransmitter serotonin (5-hydroxytryptamine; 5-HT); however, the relevant downstream signalling pathways are incompletely understood. Recently, the melanocortin system, a nexus for multiple modulators of energy balance, has emerged as one key mediator of serotonin's effects on appetite. Here we review the serotonin and melanocortin systems with reference to their roles in energy balance and discuss the evidence that the two systems are functionally linked.


Subject(s)
Energy Metabolism , Melanocortins/metabolism , Serotonin/metabolism , Animals , Humans , Obesity/metabolism , Serotonin/biosynthesis
4.
Pharmacol Biochem Behav ; 97(1): 84-91, 2010 Nov.
Article in English | MEDLINE | ID: mdl-20837046

ABSTRACT

An inverse relationship between brain serotonin and food intake and body weight has been known for more than 30 years. Specifically, augmentation of brain serotonin inhibits food intake, while depletion of brain serotonin promotes hyperphagia and weight gain. Through the decades, serotonin receptors have been identified and their function in the serotonergic regulation of food intake clarified. Recent refined genetic studies now indicate that a primary mechanism through which serotonin influences appetite and body weight is via serotonin 2C receptor (5-HT(2C)R) and serotonin 1B receptor (5-HT(1B)R) influencing the activity of endogenous melanocortin receptor agonists and antagonists at the melanocortin 4 receptor (MC4R). However, other mechanisms are also possible and the challenge of future research is to delineate them in the complete elucidation of the complex neurocircuitry underlying the serotonergic control of appetite and body weight.


Subject(s)
Brain/metabolism , Eating/physiology , Receptors, Serotonin/metabolism , Serotonin/metabolism , Animals , Body Weight/physiology , Eating/psychology , Feeding Behavior/physiology , Feeding Behavior/psychology , Humans
5.
Trends Endocrinol Metab ; 20(5): 203-15, 2009 Jul.
Article in English | MEDLINE | ID: mdl-19541496

ABSTRACT

The rise in the global prevalence of human obesity has emphasized the need for a greater understanding of the physiological mechanisms that underlie energy homeostasis. Numerous circulating nutritional cues and central neuromodulatory signals are integrated within the brain to regulate both short- and long-term nutritional state. The central melanocortin system represents a crucial point of convergence for these signals and, thus, has a fundamental role in regulating body weight. The melanocortin ligands, synthesized in discrete neuronal populations within the hypothalamus and brainstem, modulate downstream homeostatic signalling via their action at central melanocortin-3 and -4 receptors. Intimately involved in both ingestive behaviour and energy expenditure, the melanocortin system has garnered much interest as a potential therapeutic target for human obesity.


Subject(s)
Energy Metabolism/physiology , Melanocortins/metabolism , Energy Intake/physiology , Humans , Signal Transduction/physiology
7.
Mol Brain ; 1: 19, 2008 Dec 03.
Article in English | MEDLINE | ID: mdl-19055781

ABSTRACT

Temporal control of brain and behavioral states emerges as a consequence of the interaction between circadian and homeostatic neural circuits. This interaction permits the daily rhythm of sleep and wake, regulated in parallel by circadian cues originating from the suprachiasmatic nuclei (SCN) and arousal-promoting signals arising from the orexin-containing neurons in the tuberal hypothalamus (TH). Intriguingly, the SCN circadian clock can be reset by arousal-promoting stimuli while activation of orexin/hypocretin neurons is believed to be under circadian control, suggesting the existence of a reciprocal relationship. Unfortunately, since orexin neurons are themselves activated by locomotor promoting cues, it is unclear how these two systems interact to regulate behavioral rhythms. Here mice were placed in conditions of constant light, which suppressed locomotor activity, but also revealed a highly pronounced circadian pattern in orexin neuronal activation. Significantly, activation of orexin neurons in the medial and lateral TH occurred prior to the onset of sustained wheel-running activity. Moreover, exposure to a 6 h dark pulse during the subjective day, a stimulus that promotes arousal and phase advances behavioral rhythms, activated neurons in the medial and lateral TH including those containing orexin. Concurrently, this stimulus suppressed SCN activity while activating cells in the median raphe. In contrast, dark pulse exposure during the subjective night did not reset SCN-controlled behavioral rhythms and caused a transient suppression of neuronal activation in the TH. Collectively these results demonstrate, for the first time, pronounced circadian control of orexin neuron activation and implicate recruitment of orexin cells in dark pulse resetting of the SCN circadian clock.


Subject(s)
Circadian Rhythm/physiology , Darkness , Intracellular Signaling Peptides and Proteins/metabolism , Neurons/metabolism , Neuropeptides/metabolism , Animals , Behavior, Animal , Circadian Rhythm/radiation effects , Hypothalamus/cytology , Hypothalamus/metabolism , Immunohistochemistry , Male , Mice , Mice, Inbred C57BL , Models, Biological , Neurons/cytology , Neurons/radiation effects , Orexins , Proto-Oncogene Proteins c-fos/metabolism , Raphe Nuclei/cytology , Raphe Nuclei/metabolism , Suprachiasmatic Nucleus/cytology , Suprachiasmatic Nucleus/metabolism
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