ABSTRACT
A direct method using 99mtechnecium to label the synthetic antimicrobial oligopeptide ubiquicidine with a molecular mass of 1.7 kDa has been developed. The radiochemical purity of the resultant labeled compound was more than 98%. A combination of ubiquicidine and 99mtechnecium showed stability in blood plasma. The biological study indicated that the complex was renally excreted from blood flow rapidly (the half-life is 30 min). It accumulated in the experimental inflammatory focus of laboratory animals, peaking 1-2 hours after intravenous injection when the inflammatory focus/intact tissue ratio amounted up to 2.6-3.4, which could yield satisfactory abscess scintigrams. The findings make it possible to consider ubiquicidine as a promising compound for the design of radiopharmaceutical to image inflammatory foci.
Subject(s)
Radiopharmaceuticals , Ribosomal Proteins/pharmacokinetics , Sodium Pertechnetate Tc 99m , Staphylococcal Infections , Animals , Disease Models, Animal , Injections, Intravenous , Radionuclide Imaging , Radiopharmaceuticals/administration & dosage , Radiopharmaceuticals/pharmacokinetics , Rats , Rats, Wistar , Ribosomal Proteins/therapeutic use , Sodium Pertechnetate Tc 99m/administration & dosage , Sodium Pertechnetate Tc 99m/pharmacokinetics , Staphylococcal Infections/diagnostic imaging , Staphylococcal Infections/drug therapy , Staphylococcal Infections/metabolism , Staphylococcus/pathogenicity , Treatment OutcomeABSTRACT
IgG diagnosticum for measuring the concentration of 131I-labeled IgG antibodies to enteric antigen beta 1MA by the latex agglutination inhibition (LAI) test has been prepared on the basis of polyacrolein latexes. A method for the titration of anti-IgG antibodies with the use of the above diagnosticum has been developed, based on the late, agglutination (LA) test. The optimum conditions for the microtitration variant of the LA and LAI tests have been defined. High sensitivity, specificity and simplicity of analysis with the use of latex IgG diagnosticum have been demonstrated. The newly developed methods have been successfully used in laboratory trials of a new diagnostic radiopharmaceuticals for the assay of 131I-labeled antibodies in this preparation and for the detection of side effects of immunization on the recipients.
Subject(s)
Immunoglobulin G/analysis , Latex Fixation Tests/methods , Acrolein , Animals , Antigens/immunology , Dogs , Evaluation Studies as Topic , Humans , Latex , Latex Fixation Tests/instrumentation , Meconium/immunology , Mice , Particle Size , Polymers , Rabbits , RatsABSTRACT
It has been shown by direct radiometry that 99m Tc-pertechnetate is excreted from the rat testes according to the exponential dependence, the maximum accumulation being observed 10 minutes after the injection (0.5% per 1 g tissue of the injected activity). Autoradiography has revealed that most of the radionuclide is located in the vascular system of the testes, whilst the minor part in the seminiferous tubules, the maximum accumulation being reached one hour after injection. It is concluded that 99mTc-pertechnetate behaviour pattern in the testes is first of all determined by its content in the vascular system of the organ.
