ABSTRACT
Materials used for wound care have evolved from simple covers to functional wound dressings with bioactive properties. Electrospun nanofibers show great similarity to the natural fibrillar structure of skin extracellular matrix (ECM); therefore, by mimic, the morphology of ECM, nanofibers show high potential for facilitating the healing of skin injuries. Besides morphology, scaffold composition is another important parameter in the production of bioactive wound dressings. Collagen type I is the main structural protein of skin ECM is biocompatible, biodegradable, and its extraction from animal sources is relatively simple. The fabrication of electrospun wound dressings based on collagen and its blends have been studied for skin tissue engineering applications. This review focus on the new advances of collagen electrospun materials for skin wound treatment. It summarizes the recent research on pristine collagen, collagen blends, and collagen surface modifications on nanofibers mats. Finally, the strategies for three-dimensional nanofibers production will also be discussed.
Subject(s)
Collagen/pharmacology , Skin/pathology , Tissue Engineering , Tissue Scaffolds/chemistry , Wound Healing/drug effects , Animals , Collagen/chemistry , Humans , Skin/drug effectsABSTRACT
BACKGROUND: Decatropis bicolor (Zucc.)Radlk is a plant that has been traditionally used for the treatment of breast cancer in some communities of Mexico. So, the aim of this study was to determine the cytotoxic and apoptotic effect of the essential oil of Decatropis bicolor against breast cancer cell line, MDA-MB-231. METHODS: The essential oil obtained from hydrodestillation of leaves of Decatropis bicolor was studied for its biological activity against breast cancer cells MDA-MB-231 by MTT assay, Hematoxylin-eosin stain, Annexin V-FITC, TUNEL and western blot assays and for its chemical composition by GC-MS. RESULTS: The results showed a relevant cytotoxic effect of the essential oil towards MDA-MB-231 cells in a dose- and time- dependent manner, with an IC50 of 53.81 ± 1.691 µg/ml but not in the epithelial mammary cell line MCF10A (207.51 ± 3.26 µg/ml). Morphological examination displayed apoptotic characteristics in the treated cells like cell size reduction, membrane blebbing and apoptotic bodies. In addition, the apoptotic rate significantly increased as well as DNA fragmentation and western blot analysis revealed that the essential oil induced apoptosis in the MDA-MB-231 cells via intrinsic pathways due to the activation of Bax, caspases 9 and 3. Phytochemical analysis of the Decatropis bicolor essential oil showed the presence of twenty-three compounds. Major components of the oil were 1,5-cyclooctadiene,3-(methyl-2)propenyl (18.38 %), ß-terpineol (8.16 %) and 1-(3-methyl-cyclopent-2-enyl)-cyclohexene (6.12 %). CONCLUSIONS: This study suggests that essential oil of Decatropis bicolor has a potential cytotoxic and antitumoral effect against breast cancer cells, with the presence of potential bioactive compounds. Our results contribute to the validation of the anticancer activity of the plant in Mexican traditional medicine.
Subject(s)
Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Oils, Volatile/pharmacology , Plant Extracts/pharmacology , Rutaceae/chemistry , Antineoplastic Agents/chemistry , Cell Line, Tumor , Cell Survival/drug effects , Humans , Oils, Volatile/chemistry , Plant Extracts/chemistryABSTRACT
Water vapor diffusion coefficient (WVDC) and thermal diffusivity (alpha) were determined in gelatin-starch films through photothermal techniques. The effect of different variables in the elaboration of these films, such as starch and glycerol concentrations and pH, were evaluated through the response surface methodology. The results indicated that an increase in the glycerol concentration and pH favored the WVDC of the films. On the other hand, alpha was influenced principally by the starch content and pH of the film-forming solution. The minimum alpha value was 4.5 x 10(-4) cm2/s, which is compared with alpha values reported for commercial synthetic polymers.
Subject(s)
Food Packaging , Starch/chemistry , Temperature , Water/chemistry , Acoustics/instrumentation , Diffusion , Gelatin/chemistry , Glycerol/chemistry , Hydrogen-Ion Concentration , Light , Polymers/chemistry , PorositySubject(s)
Kidney Failure, Chronic/therapy , Kidney Transplantation , Registries , Renal Dialysis , Adolescent , Adult , Aged , Humans , Kidney Failure, Chronic/epidemiology , Kidney Transplantation/statistics & numerical data , Middle Aged , Renal Dialysis/statistics & numerical data , Spain/epidemiologyABSTRACT
No disponible
Subject(s)
Adult , Aged , Adolescent , Humans , Renal Insufficiency, Chronic/therapy , Kidney Transplantation/statistics & numerical data , Registries , Renal Dialysis/statistics & numerical data , Renal Insufficiency, Chronic/epidemiology , Spain/epidemiologySubject(s)
Kidney Transplantation/statistics & numerical data , Registries , Renal Dialysis/statistics & numerical data , Adolescent , Adult , Aged , Child , Child, Preschool , Humans , Incidence , Infant , Kidney Failure, Chronic/epidemiology , Kidney Failure, Chronic/etiology , Kidney Failure, Chronic/therapy , Middle Aged , Prevalence , SpainSubject(s)
Kidney Transplantation/statistics & numerical data , Peritoneal Dialysis/statistics & numerical data , Renal Dialysis/statistics & numerical data , Adolescent , Adult , Age Distribution , Aged , Cardiovascular Diseases/mortality , Child , Child, Preschool , Female , Global Health , Humans , Incidence , Infant , Infections/mortality , Kidney Diseases/complications , Kidney Diseases/epidemiology , Kidney Failure, Chronic/epidemiology , Kidney Failure, Chronic/etiology , Kidney Failure, Chronic/therapy , Kidney Transplantation/mortality , Male , Middle Aged , Neoplasms/mortality , Peritoneal Dialysis/mortality , Prevalence , Registries , Renal Dialysis/mortality , Retrospective Studies , Societies, Medical , Spain , Surveys and QuestionnairesABSTRACT
No disponible
