ABSTRACT
Approximately 40 genotypes of the human papillomavirus (HPV) have been identified in cervical mucosa. In particular, HPV-16 and HPV-18 have been associated with cervical neoplasia. Squamous intraepithelial lesions (SILs) are precursors of cervical cancer. This study aimed to identify the HPV by genotype in SILs using a linear array genotyping test in a population in Mexico. We performed a cross-sectional study of 129 female patients with or without SILs, as determined by colposcopy, who completed a risk factor questionnaire. Cervical swab samples were obtained and genotyped using a Linear Array HPV Genotyping assay. Forty-nine (37.98%) samples were positive for HPV, and 24 genotypes were identified among these samples. The most common genotype was HPV-16. Twelve genotypes were found in both high- and low-grade SILs (HPV-6, 16, 31, 39, 51, 52, 53, 58, 59, 61, 67, and 84), of which seven were high-risk SILs (HPV-16, 31, 39, 51, 52, 58, and 59). Among the populations studied, the most frequent genotype was HPV-16, multiple infections were found, and four patients without injury tested positive for HPV.
Subject(s)
Papillomaviridae/genetics , Papillomavirus Infections/virology , Squamous Intraepithelial Lesions/virology , Uterine Cervical Dysplasia/virology , Adult , Cervix Uteri/pathology , Cervix Uteri/virology , Cross-Sectional Studies , DNA, Viral/genetics , Female , Genotype , Genotyping Techniques , Humans , Mexico , Papillomaviridae/classification , Papillomavirus Infections/epidemiology , Risk Factors , Uterine Cervical Neoplasms/virologyABSTRACT
INTRODUCCIÓN: El objetivo del presente trabajo fue medir la expresión de interferón gamma en la infección por VPH y por Chlamydia trachomatis en pacientes con lesión intraepitelial escamosa. MÉTODO: Se incluyeron 100 muestras de pacientes diagnosticadas por colposcopía, con y sin lesión intraepitelial escamosa, en quienes se efectuó el diagnóstico de infección por VPH y/o C. trachomatis. Se cuantificó la expresión relativa de interferón gamma con la prueba de transcriptasa reversa-PCR en tiempo real (RT-PCR). RESULTADOS: Las unidades relativas de la expresión de interferón gamma fueron de 13, 1,8 y 0,3 en la coinfección por VPH y C. trachomatis, en la infección por VPH y en la infección por C. trachomatis, respectivamente. CONCLUSIÓN: La infección por VPH y por C. trachomatis puede constituir un factor estimulante de la expresión de interferón gamma
INTRODUCTION: The aim of this study was to mesure the expression of gamma interferon in HPV and Chlamydia trachomatis infection in squamous intraepithelial lesions. METHOD: Samples from 100 patients diagnosed by colposcopy with or without squamous intraepithelial lesions were used in the present study. Each patient was found to be infected by HPV and C. trachomatis. Relative gamma interferon mRNA expression was assessed using a real-time reverse transcriptase PCR assay (RT-PCR). RESULTS: The relative units of expression of gamma interferon mRNA were 13, 1.8 and 0.3, for HPV and C. trachomatis co-infection, or HPV or C. trachomatis infection, respectively. CONCLUSIÓN: HPV and C. trachomatis could overstimulate the expression of gamma interferon
Subject(s)
Humans , Interferon-gamma/analysis , Papillomavirus Infections/immunology , Chlamydia Infections/immunology , Uterine Cervical Neoplasms/microbiology , Papillomaviridae/pathogenicity , Chlamydia trachomatis/pathogenicity , Coinfection/microbiology , Sexually Transmitted Diseases/immunology , 31574/microbiologyABSTRACT
INTRODUCTION: The aim of this study was to mesure the expression of gamma interferon in HPV and Chlamydia trachomatis infection in squamous intraepithelial lesions. METHOD: Samples from 100 patients diagnosed by colposcopy with or without squamous intraepithelial lesions were used in the present study. Each patient was found to be infected by HPV and C.trachomatis. Relative gamma interferon mRNA expression was assessed using a real-time reverse transcriptase PCR assay (RT-PCR). RESULTS: The relative units of expression of gamma interferon mRNA were 13, 1.8 and 0.3, for HPV and C.trachomatis co-infection, or HPV or C.trachomatis infection, respectively. CONCLUSION: HPV and C.trachomatis could overstimulate the expression of gamma interferon.
Subject(s)
Chlamydia Infections/metabolism , Chlamydia trachomatis , Interferon-gamma/biosynthesis , Papillomavirus Infections/metabolism , Squamous Intraepithelial Lesions of the Cervix/microbiology , Adult , Chlamydia Infections/complications , Cross-Sectional Studies , Female , Humans , Papillomavirus Infections/complications , Squamous Intraepithelial Lesions of the Cervix/complicationsABSTRACT
Granulosa cell tumor of the ovary is an uncommon neoplasm characterized by a long natural history, late recurrence capacity and good prognosis. We report the case of a woman with adult-type granulosa cell tumor of the ovary, which developed late recurrence with contralateral ovarian disease, intra-abdominal spread and liver metastases.
