[Stromal cell transplant in the 6-OHDA lesion model]. / Trasplante de células estromales en el modelo de lesión por 6-OHDA.

INTRODUCTION: A good deal of evidence currently exists to show that transplanting foetal mesencephalic tissue can produce symptomatic benefits both in patients and in disease models. Nevertheless, the technical and ethical difficulties involved in obtaining enough suitable foetal cerebral tissue have been a serious obstacle to its application. Stromal cells derived from bone marrow, due to their potential capacity to generate different types of cells, could be an ideal source of material for cell restoration in neurodegenerative diseases. AIMS: Our aim was to evaluate the effect of transplanting stromal cells derived from bone marrow on the behaviour of 6-OHDA rats, when they are inserted into the striatum. MATERIAL AND METHODS: In this study we used rats with a lesion in the substantia nigra induced by 6-hydroxydopamine, divided into several experimental groups. Rotary activity induced by D-amphetamine (5 mg/kg, intraperitoneally) was evaluated before and throughout the three months following the transplant in all the experimental groups, except in the group of healthy controls. Hemiparkinsonian rats received a total of 350 000 foetal ventral mesencephalic cells and 8 x 10(4) stromal cells/microL, which were implanted in the striatum. RESULTS AND CONCLUSIONS: Animals with stromal cells transplanted in the body of the striatum significantly reduced the number of turns induced by amphetamine (p < 0.05); yet this reduction was not greater than that induced by foetal mesencephalic cell transplants. We were also unable to demonstrate any significant improvement in the motor skills of the forelimbs.

Trasplante de células estromales en el modelo de lesión por 6-OHDA / Stromal cell transplant in the 6-OHDA lesion model

A good deal of evidence currently exists to show that transplanting foetal mesencephalic tissue can produce symptomatic benefits both in patients and in disease models. Nevertheless, the technical and ethical difficulties involved in obtaining enough suitable foetal cerebral tissue have been a serious obstacle to its application. Stromal cells derived from bone marrow, due to their potential capacity to generate different types of cells, could be an ideal source of material for cell restoration in neurodegenerative diseases. AIMS: Our aim was to evaluate the effect of transplanting stromal cells derived from bone marrow on the behaviour of 6-OHDA rats, when they are inserted into the striatum(AU)

Trasplante de células estromales en el modelo de lesión por 6-OHDA / Stromal cell transplant in the 6-OHDA lesion model

Introducción. En la actualidad, existe un cúmulo de evidencias de que el trasplante de tejido mesencefálico fetal puede producir un beneficio sintomático tanto en los pacientes con enfermedad de Parkinson como en los modelos de la enfermedad. Sin embargo, las dificultades técnicas y éticas en la obtención de tejido cerebral fetal apropiado y en cantidad suficiente ha dificultado su aplicación. Las células estromales derivadas de médula ósea, debido a su potencialidad para generar diferentes tipos de células, podrían ser una fuente ideal para la restauración celular en las enfermedades neurodegenerativas. Objetivo. Evaluar el efecto del trasplante de células estromales derivadas de médula ósea sobre la conducta de ratas con lesión por 6-OHDA, cuando se realiza en el estriado. Materiales y métodos. Se utilizaron ratas con lesión de la sustancia negra inducida por la 6-OHDA, divididas en varios grupos experimentales. La actividad rotatoria inducida por D-anfetamina (5 mg/kg intraperitonialmente) se evaluó antes y en los tres meses posteriores al trasplante en todos los grupos experimentales, excepto en el grupo de controles sanas. Las ratas hemiparkinsonianas recibieron un total de 350.000 células de mesencéfalo ventral fetal y 8 × 104 células estromales/µL, las cuales se implantaron en el estriado. Resultados y conclusiones. Los animales con trasplante de células estromales en el cuerpo estriado redujeron significativamente el número de vueltas inducidas por anfetamina (p < 0,05); sin embargo, esta reducción no fue mayor que la inducida por los trasplantes de células mesencefálicas fetales. Por otro lado, no fue posible demostrar una mejoría significativa de las habilidades motoras de las extremidades anteriores (AU)

Introduction. A good deal of evidence currently exists to show that transplanting foetal mesencephalic tissue can produce symptomatic benefits both in patients and in disease models. Nevertheless, the technical and ethical difficulties involved in obtaining enough suitable foetal cerebral tissue have been a serious obstacle to its application. Stromal cells derived from bone marrow, due to their potential capacity to generate different types of cells, could be an ideal source of material for cell restoration in neurodegenerative diseases. Aims. Our aim was to evaluate the effect of transplanting stromal cells derived from bone marrow on the behaviour of 6-OHDA rats, when they are inserted into the striatum. Materials and methods. In this study we used rats with a lesion in the substantia nigra induced by 6-hydroxydopamine, divided into several experimental groups. Rotary activity induced by D-amphetamine (5 mg/kg, intraperitoneally) was evaluated before and throughout the three months following the transplant in all the experimental groups, except in the group of healthy controls. Hemiparkinsonian rats received a total of 350,000 foetal ventral mesencephalic cells and 8 × 104 stromal cells/µL, which were implanted in the striatum. Results and conclusions. Animals with stromal cells transplanted in the body of the striatum significantly reduced the number of turns induced by amphetamine (p < 0.05); yet this reduction was not greater than that induced by foetal mesencephalic cell transplants. We were also unable to demonstrate any significant improvement in the motor skills of the forelimbs (AU)