ABSTRACT
The aims were to assess the prevalence of resistances to antiretroviral drugs in HIV patients in the province of Cuenca and to determine the most frequently detected primary resistances. A total of 79 samples collected from April 2000 to July 2004 were analyzed. Resistance was detected using the genotypic technique VERSANTTM HIV1 (LiPA). Mutations more frequently found were M184V in the reverse transcriptase gene and V82A in the protease gene. The prevalence of primary resistances was 39.4%. Mutations more frequently detected were similar to those reported by other authors. The rate of primary resistance was higher than those found by other authors in Spain.
Subject(s)
Anti-HIV Agents/pharmacology , Drug Resistance, Viral/genetics , HIV Infections/virology , HIV-1/drug effects , Amino Acid Substitution , Drug Resistance, Multiple, Viral/genetics , Female , Genotype , HIV Infections/epidemiology , HIV Protease/genetics , HIV Reverse Transcriptase/genetics , HIV-1/genetics , Humans , Male , Mutation, Missense , Point Mutation , Prevalence , RNA, Viral/genetics , Spain/epidemiologyABSTRACT
Los objetivos del estudio fueron determinar la prevalencia de la resistencia a los fármacos antirretrovirales en pacientes VIH positivos en la provincia de Cuenca y describir las resistencias primarias observadas con más frecuencia. Se analizaron en total 79 muestras remitidas desde abril de 2000 a julio de 2004. Las resistencias se detectaron utilizando la técnica genotípica VERSANTTM HIV1 (LiPA). Las mutaciones que más se detectaron fueron M184V para la región de la transcriptasa inversa y V82A para la región de la proteasa. La prevalencia de las resistencias primarias fue del 39,4%. Las mutaciones más observadas son similares a las detectadas por otros autores. El porcentaje de pacientes con resistencias primarias es superior a lo hallado por otros autores en España
The aims were to assess the prevalence of resistances to antiretroviral drugs in HIV patients in the province of Cuenca and to determine the most frequently detected primary resistances. A total of 79 samples collected from April 2000 to July 2004 were analyzed. Resistance was detected using the genotypic technique VERSANTTM HIV1 (LiPA). Mutations more frequently found were M184V in the reverse transcriptase gene and V82A in the protease gene. The prevalence of primary resistances was 39.4%. Mutations more frequently detected were similar to those reported by other authors. The rate of primary resistance was higher than those found by other authors in Spain
Subject(s)
Male , Female , Adult , Humans , Genotype , Anti-Retroviral Agents/pharmacology , Acquired Immunodeficiency Syndrome/drug therapy , Acquired Immunodeficiency Syndrome/complications , AIDS-Related Opportunistic Infections/drug therapy , Epidemiology, Descriptive , Acquired Immunodeficiency Syndrome/genetics , Mutation/genetics , DNA-Directed RNA Polymerases/genetics , Spain/epidemiologyABSTRACT
Two cases of meningitis due to Listeria monocytogenes in a 73-year-old man and a 77-year-old woman are described. Both patients were admitted to an Emergency Department in acute state, probably caused by cerebral stroke. Neither presented clinical symptoms of meningitis. No other concomitant conditions which might weaken the patient's general state were known to be present. The first patient died after 16 hours, the second after 5. The analysis of the cerebrospinal fluid gave the following information: Case 1: 1,500 cells, 80 percent of which were polynuclear neutrophils; Pandy's reaction was positive; albumin 1.5 g/l and glucose 0.65 g/l. Case 2: 197 cells/mm3, 90 percent of which were polynuclear neutrophils; Pandy's reaction was positive; albumin and glucose were 0.60 and 0.10 g/l respectively. Samples of the cerebrospinal fluid were cultured in various media and pure cultures of Listeria monocytogenes, subtype 4b, were isolated. The source of the infection could not be determined in either of the two cases.
