ABSTRACT
Carbon tetrachloride (CCl4) is a known environmental biohazard, which induces lipid peroxidation (LPO) and oxidative damage in rat liver. In this study, the hepatoprotective effect of Gossypitrin, a flavonoid extracted from Hibiscus elatus S.W, was investigated against the CCl4-induced in vivo hepatotoxicity. The levels of malondialdehyde (MDA) were assayed as an index of LPO and the levels of catalase (CAT) activity as a biomarker of oxidative damage. Leakage of aspartate aminotransferase (ALT) and lactate dehydrogenase (LDH), liver weight/body weight ratio as well as morphological parameters were used as signs of hepatotoxicity. CCl4 (1 ml/kg), intraperitoneally injected into rats, caused increased MDA production and CAT activity, and also a significant ALT and LDH leakage as compared to levels of these constituents in the control group. Changes in morphology, including steatosis, cells forming balloon cells and necrosis were evaluated in the hepatotoxin-induced damage. Treatment of rats with Gossypitrin (3.98, 5.97 and 8.95 mg/kg) 2 h before and 2 h after CCl4 injection, protected hepatocytes against cell injury induced by CCl4 and its efficacy as an antioxidant was similar to vitamin E (used as a reference antioxidant). These results are consistent with the conclusion that the toxicity of CCl4 is due to LPO and the generation of reactive oxygen species (ROS), and that Gossypitrin's protective effects relate to its direct radical scavenging ability and other antioxidative processes induced by its structure.
Subject(s)
Carbon Tetrachloride/toxicity , Flavonoids/pharmacology , Flowers/chemistry , Hibiscus/chemistry , Liver/drug effects , Plant Preparations/pharmacology , Animals , Biomarkers , Body Weight , Carbon Tetrachloride/pharmacology , Female , Flavonoids/chemistry , Liver/metabolism , Liver/pathology , Molecular Structure , Organ Size , Plant Preparations/chemistry , Rats , Rats, Sprague-Dawley , Thiobarbituric Acid Reactive Substances/metabolismABSTRACT
Las antraciclinas son fármacos antineoplásicos de probada eficacia clínica, aunque ésta se halla limitada por su cardiotoxicidad. Para este trabajo, estudio piloto diseñado para determinar si los indicadores del estrés oxidativo pudieran ser marcadores tempranos del daño miocárdico, inducido por una de estas antraciclinas: la doxorrubicina, se seleccionó una muestra de 12 niños, divididos en 4 grupos. Estos fueron monitoreados con electrocardiogramas y ecocardiogramas, los valores de transaminasas, se les determinaron los niveles de malonildialdehído y glutatión reducido antes del tratamiento y después del segundo ciclo quimioterapéutico. Estos resultados, no permitieron arribar a conclusiones finales. Sólo se puede señalar que las concentraciones, evaluadas como indicadores del estrés oxidativo, parecen ser los candidatos potenciales para la detección temprana del daño celular inducido por adriamicina, además, la vitamina E, como agente antioxidante, mostró cierta protección frente al desafío tóxico inducido por doxorrubicina, sustentado esto en los hallazgos obtenidos con relación a las concentraciones de malonildialdehido y glutatión reducido(AU)
