Response of Muscle Damage Markers to an Accentuated Eccentric Training Protocol: Do Serum and Saliva Measurements Agree?

ABSTRACT: González-Hernández, JM, Jiménez-Reyes, P, Cerón, JJ, Tvarijonaviciute, A, Llorente-Canterano, FJ, Martínez-Aranda, LM, and García-Ramos, A. Response of muscle damage markers to an accentuated eccentric training protocol: do serum and saliva measurements agree? J Strength Cond Res 36(8): 2132-2138, 2022-This study aimed (a) to examine the acute and delayed responses of 3 muscle damage biomarkers: creatine kinase (CK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) to an accentuated eccentric training protocol in serum, and (b) to explore the changes of these biomarkers in saliva and compare them with serum. Sixteen resistance-trained university students (10 men [age = 26.6 ± 4.8 years, full squat one repetition maximum [1RM] = 103.4 ± 14.4 kg] and 6 women [age = 22.7 ± 1.4 years, full squat estimated 1RM = 68.3 ± 10.5 kg]) completed an accentuated eccentric strength training protocol with the full squat exercise consisting of 8 sets of 10 repetitions against the 120% estimated 1RM load with 5 minutes of interset rest. The activity of muscle damage biomarkers (CK, AST, and LDH) was measured in serum and saliva before training (Pre), 24 hours after training (Post24), and 96 hours after training (Post96). In serum, lower values of the 3 muscle damage markers were observed at Pre compared to Post24 and Post96, whereas no significant differences were observed between Post24 and Post96 for any analyte. In saliva, there was a significant increase in men at Post96 compared with Pre in CK. The correlations between the measurements in serum and saliva ranged from trivial to small ( r = -0.034 to 0.212). These results suggest that the measurement of muscle damage markers in serum and saliva do not provide the same information in the conditions of our study.

Differences between adjusted vs. non-adjusted loads in velocity-based training: consequences for strength training control and programming.

Strength and conditioning specialists commonly deal with the quantification and selection the setting of protocols regarding resistance training intensities. Although the one repetition maximum (1RM) method has been widely used to prescribe exercise intensity, the velocity-based training (VBT) method may enable a more optimal tool for better monitoring and planning of resistance training (RT) programs. The aim of this study was to compare the effects of two RT programs only differing in the training load prescription strategy (adjusting or not daily via VBT) with loads from 50 to 80% 1RM on 1RM, countermovement (CMJ) and sprint. Twenty-four male students with previous experience in RT were randomly assigned to two groups: adjusted loads (AL) (n = 13) and non-adjusted loads (NAL) (n = 11) and carried out an 8-week (16 sessions) RT program. The performance assessment pre- and post-training program included estimated 1RM and full load-velocity profile in the squat exercise; countermovement jump (CMJ); and 20-m sprint (T20). Relative intensity (RI) and mean propulsive velocity attained during each training session (Vsession) was monitored. Subjects in the NAL group trained at a significantly faster Vsession than those in AL (p < 0.001) (0.88-0.91 vs. 0.67-0.68 m/s, with a ∼15% RM gap between groups for the last sessions), and did not achieve the maximum programmed intensity (80% RM). Significant differences were detected in sessions 3-4, showing differences between programmed and performed Vsession and lower RI and velocity loss (VL) for the NAL compared to the AL group (p < 0.05). Although both groups improved 1RM, CMJ and T20, NAL experienced greater and significant changes than AL (28.90 vs.12.70%, 16.10 vs. 7.90% and -1.99 vs. -0.95%, respectively). Load adjustment based on movement velocity is a useful way to control for highly individualised responses to training and improve the implementation of RT programs.

Chronic effects and optimal dosage of strength training on SBP and DBP: a systematic review with meta-analysis.

BACKGROUND AND OBJECTIVES: Hypertension is a disease affecting a large part of the world's population that causes millions of deaths annually. Physical exercise is proposed as an alternative to pharmacologic therapies used to reduce blood pressure. The main objective was to compare the effect of different types of strength training in blood pressure, as well as to analyse several variables that can modify the effect of strength training not combined with medication in SBP and DBP (SBP-DBP). METHODS: The search was carried out in two scientific databases: PubMed and Web of Science. Articles were included following three criteria: analysing the chronic effect of strength training in blood pressure; the studies were conducted at least during 4 weeks; and the articles were published in English. RESULTS: The analysis showed a significant decrease of blood pressure for all types of training. The effect on SBP was greater when training without medication was carried out with isometric exercises than when training was performed with dynamic exercises. Moreover, the effects were no longer significant when the duration of the training programme exceeded 20 weeks as well as when training frequency was lower than three times per week were found. CONCLUSION: Strength training is effective in reducing both blood pressures (SBP-DBP). Training programmes, consisting of dynamic strength training without medication at a moderate intensity and with a frequency of three times per week, seem to be optimal in order to reduce blood pressure.Prospective register of Systematic Reviews: CRD42019122421.

Evolution of contractile properties of the lower limb muscles throughout a season in elite futsal players.

BACKGROUND: Futsal is a team sport involving intermittent technical actions of high intensity, and high physical (strength) and muscular demands. In this regard, the tensiomyography (TMG) is a useful and non-invasive tool for the monitoring and assessment of the muscle's contractile capacity. This study aimed to analyze the changes in the contractile properties produced during the season, as well as to determine the potential cumulative effect of a resistance training (RT) program in futsal players. METHODS: Fourteen elite futsal players (2 goalkeepers, 4 defenders, 4 wingers and 3 pivots) were assessed by TMG at 11th, 18th, and 28th week of the season. The maximal radial displacement of the muscle belly (Dm); contraction time (Tc); delay time (Td) and radial displacement velocity (90%) Dm (VrD90) were assessed. After the second measurement, a RT program was included in the regular training sessions and focused on the lower body musculature. It was performed during 9 weeks (1-weekly). Finally, a third measurement was performed between 28th-29th weeks. Repeated measures analysis of variance was used to detect in-season changes. Two factors were included: Time (changes detected after resistance training program) was used as the within-subject factor and the specific position was used as the between-subject factor. RESULTS: An increment in Tc for several muscles: biceps femoris (BF; P=0.02), semitendinosus (ST; P=0.04), adductor longus (AL; P=0.008) and gastrocnemius medialis (GM; P=0.009) was observed throughout the season. Similarly, significant increments in Dm for GM (P=0.02) and AL (P=0.05), as well as increments in Td for BF (P=0.002) were found. Moreover, no significant changes in VrD90 between time points 2-3 (analysis of RT effect) were observed. Additionally, the player´s positions reported no significant changes for any of the variables analyzed. CONCLUSIONS: An increase respect to baseline levels was observed for Tc, Td and Dm during the season. However, the adaptations to contractile properties were muscle specific. In addition, an in-season 9-week RT program (1-weekly), had no significant effects (time points 2-3) on the contractile properties of futsal players. In addition, there were no differences when comparing different positions.

Effect of Sleep Quality on the Prevalence of Sarcopenia in Older Adults: A Systematic Review with Meta-Analysis.

Sarcopenia is an age-related condition. However, the prevalence of sarcopenia may increase due to a range of other factors, such as sleep quality/duration. Therefore, the aim of the study is to conduct a systematic review with meta-analysis to determine the prevalence of sarcopenia in older adults based on their self-reported sleep duration. Methods: Three electronic databases were used-PubMed-Medline, Web of Science, and Cochrane Library. We included studies that measured the prevalence of sarcopenia, divided according to sleep quality and excluded studies (a) involving populations with neuromuscular pathologies, (b) not showing prevalence values (cases/control) on sarcopenia, and (c) not including classificatory models to determine sleep quality. Results: high prevalence values in older adults with both long and short sleep duration were shown. However, prevalence values were higher in those with inadequate sleep (<6-8 h or low efficiency) (OR 0.76; 95% CI (0.70-0.83); Q = 1.446; p = 0.695; test for overall effect, Z = 6.01, p < 0.00001). Likewise, higher prevalence levels were shown in men (OR 1.61; 95% CI (0.82-3.16); Q = 11.80; p = 0.0189) compared to women (OR 0.77; 95% CI (0.29-2.03); Q = 21.35; p = 0.0003). Therefore, the prevalence of sarcopenia appears to be associated with sleep quality, with higher prevalence values in older adults who have inadequate sleep.

Influence of Sampling Conditions, Salivary Flow, and Total Protein Content in Uric Acid Measurements in Saliva.

Uric acid (UA) is the most abundant antioxidant compound in saliva and one of the most sensitive biomarkers for detecting changes in the oxidative status of the organism. The aim of this study was to evaluate the effect of: (i) different methods of saliva sampling and (ii) the correction by salivary flow or total protein on UA concentrations in saliva. Paired saliva (collected by two different methods, passive drooling and using Salivette cotton rolls) and serum samples were obtained from 12 healthy men after the performance of two resistance training exercises of different level of effort that can produce different concentrations in UA in saliva. There were no significant differences between values of uric acid in saliva using Salivette and passive drool. Correlations between UA in serum and saliva and increases in UA in saliva after exercise were detected when saliva samples were obtained by passive drool and Salivette and were not corrected by salivary flow or total protein concentration. Therefore for UA measurements in saliva it would not be recommended to normalize the results by salivary flow or protein concentration. This study highlights the importance of choosing an adequate sampling method selection as well as the expression of results when analytes are measured in saliva.

Effects of Inertial Setting on Power, Force, Work, and Eccentric Overload During Flywheel Resistance Exercise in Women and Men.

Exercise load is a key component in determining end-point adaptations to resistance exercise. Yet, there is no information regarding the use of different inertia (i.e., loads) during isoinertial flywheel resistance exercise, a very popular high-intensity training model. Thus, this study examined power, work, force, and eccentric overload produced during flywheel resistance exercise with different inertial settings in men and women. Twenty-two women (n = 11) and men (n = 11) performed unilateral (in both legs) isolated concentric (CON) and coupled CON and eccentric (ECC) exercise in a flywheel knee extension device employing 6 inertias (0.0125, 0.025, 0.0375, 0.05, 0.075, 0.1 kg·m). Power decreased as higher inertias were used, with men showing greater (p ≤ 0.05) decrements than women (-36 vs. -29% from lowest to highest inertia). In contrast, work increased as higher inertias were employed, independent of sex (p ≤ 0.05; ∼48% from lowest to highest inertia). Women increased CON and ECC mean force (46-55%, respectively) more (p ≤ 0.05) than men (34-50%, respectively) from the lowest to the highest inertia evaluated, although the opposite was found for peak force data (i.e., peak force increased more in men than in women as inertia was increased). Men, but not women, increased ECC overload from inertia 0.0125 to 0.0375 kg·m2. Although estimated stretch-shorting cycle use during flywheel exercise was higher (p ≤ 0.05) in men (6.6%) than women (4.9%), values were greater for both sexes when using low-to-medium inertias. The information gained in this study could help athletes and sport and health professionals to better understand the impact of different inertial settings on skeletal muscle responses to flywheel resistance exercise.