Subgingival Microbiota and Periodontal Clinical Status in Patients with Plaque Psoriasis: A Cross-Sectional Study.

Plaque Psoriasis (PP) and periodontitis are inflammatory disorders with a bidirectional association. They both have a qualitatively similar immune-modulatory cascade, cytokine profile, and a recently described dysbiosis. Different oral bacterial species compositions in the periodontal pocket might play a role in the development of PP. To describe the subgingival microbiota of the Mexican population with PP and the periodontal conditions. Subjects were divided into two groups: periodontal health (PH) (PH-non-PP, PH-PP) and periodontitis (PD) (P-non-PP, PD-PP). Following clinical examination, the patients were classified into three groups according to the degree of psoriasis as measured by the Psoriasis Area Severity Index (PASI) and the periodontal status according to the parameters of the American Academy of Periodontology (AAP). Subgingival microbiota samples of each patient were used to determine 40 species of periodontal bacteria by checkerboard DNA-DNA hybridization. IL-2 and IL-6 were measured by ELISA. Of the forty-eight patients with PP, 21 patients had PH and 27 patients had PD. PD-PP group has a significant increase in the percentage of plaque, gingival redness, pocket probing depth, and clinical attachment loss (P<0.001) compared to PH-PP group. Microbiologically PD-PP exhibited significantly higher mean counts for A. georgiae, A. israelii, A. naeslundii from blue complex (P<0.001) than PD-non-PP. Moreover, the counts of these Actinomyces in PD-PP increased according to the severity of index PASI. The concentration of IL-2 and IL-6 were increased in saliva from PH-PP and PD-PP patients compared to PH non-PP. PP individuals harbored a particular sub-gingival microbiota profile different from non-PP. The severity of psoriasis was related to dysbiosis of microbiota -PASI > 5 related to periodontitis with the predominance of Actinomyces periodontal, irrespective of their periodontal condition. Finally, the severity of psoriasis could be unbalanced in subgingival microbiota and increase the risk to develop periodontitis.

Detection of hepatitis C virus RNA in saliva of patients with active infection not associated with periodontal or liver disease severity.

BACKGROUND: Hepatitis C virus (HCV) is mainly transmitted by parenteral route, being blood transfusion and intravenous drug use the most frequent risk factors. However, it has been suggested that there are other routes of transmission. There are several studies where HCV RNA has been detected in saliva of patients infected with HCV, and epidemiological studies have proposed the dental treatments as possible risk factors for HCV transmission. The purpose of this study was to detect the presence of HCV RNA in saliva of patients with active infection and associating with periodontal or liver disease. METHODS: Patients with quantifiable HCV-RNA in serum were enrolled in the study. Periodontal disease was assessed using the modified gingival index (MGI). Presence of dental plaque was assessed with the use of disclosing tablets. Patients were clinically and laboratory evaluated to identify the stage of liver disease, the HCV RNA was determinate in saliva by nested RT-PCR. To determine associations between different parameters univariate and multivariate analysis were used. RESULTS: A total of 45 patients were included. Of these patients, 21 (46.6%) had hepatitis, 23 (51.1%) had cirrhosis and one patient (2.4%) presented hepatocellular carcinoma (HCC). Viral loads in serum ranged from 2.31-6.68 log IU/ml with a mean of 5.46 log IU/ml (95% CI 5.23-5.70). HCV RNA was positive in saliva of 29 patients (64.4%) and was not detected in 16 (35.6%). For univariate analysis three independent variables were associated with the detection of HCV-RNA in saliva: gender, viral load and dental plaque and multivariate analysis only one independent variable viral load >5.17 log IU/mL remained significantly associated with the detection of HCV in saliva (p = 0.0002). A statistical difference was observed when viral load was analyzed, log 5.85 IU/mL (95% CI 5.67-6.02) for patients with HCV in saliva vs. log 4.77 IU/mL (95% CI 4.35-5.19) for patients without HCV in saliva (p = 0.0001). The detection of HCV-RNA in saliva was more frequent in patients with relatively high serum viral loads. CONCLUSION: HCV-RNA in saliva was associated with the level of serum viral load but not with periodontal or liver disease severity.

Necesidades de tratamiento periodontal en adultos de la región rural Mixteca del Estado de Puebla, México / Periodontal treatment needs in adults from Mixteca rural area in Puebla State, Mexico

Objetivo Determinar las necesidades de tratamiento periodontal (NTP) utilizando el índice Comunitario de Necesidades de Tratamiento Periodontal (ICNTP) en una muestra de habitantes de la región Mixteca del estado de Puebla. Material y Métodos Se realizó un estudio transversal en el que se incluyeron 50 pacientes de la clínica rural de Nativitas Cautempan, Puebla., México. Para determinar el estado de salud periodontal se utilizaron el índice Gingival y el índice Comunitario de Necesidades de Tratamiento Periodontal propuesto por la Organización Mundial de la Salud y la Federación Dental Internacional, aplicados por el mismo investigador previamente capacitado y estandarizado. En el análisis, se calculó media y desviación estándar para las variables cuantitativas y frecuencias y proporciones para las variables categóricas. Resultados La edad promedio fue 37,6± 13,6 años. El 60,0 por ciento fueron mujeres, cuya principal ocupación fue amas de casa (46,0 por ciento). El 14 por ciento fueron campesinos. El índice gingival reportó 50,0 por ciento, 32,0 por ciento y 14,0 por ciento para gingivitis leve, moderada y severa, respectivamente. Los sextantes posteriores superiores presentaron mas comúnmente bolsas de 4 a 5 mm, los sextantes anteriores, cálculo y los posteriores inferiores cálculo y hemorragia. El 94,0 por ciento de los pacientes requiere tratamiento periodontal en un promedio de 3,6 sextantes por paciente. Conclusiones Las necesidades de tratamiento periodontal fueron altas en este estudio, nueve de cada diez pacientes de la región Mixteca del Estado de Puebla lo requieren. Es necesario encaminar esfuerzos con el fin de mejorar la salud bucal en las comunidades indígenas.

Objective This study was aimed at determining periodontal treatment needs, as determined by the Community Periodontal Index of Treatment Need (CPITN), in a sample of adults from the Mixteca region of the State of Puebla, in Mexico. Materials and Methods This was a descriptive, cross-sectional, single-centre study. Previous informed consent was obtained; 60.0 percent of the sample were women whose main activity was housework (46 percent), 14.0 percent were farmers. Average age was 37.6 ± 13.6. Gingival and Community Periodontal Index of Treatment Need proposed by the World Health Organisation and the International Dental Federation were used; they were implemented by the same, previously-standardised researcher. Means, standard deviations and confidence intervals were calculated for dimensional variables and percentages for categorical ones. Results The gingival index gave 50.0 percent light gingivitis, 32.0 percent moderate and 14.0 percent severe gingivitis. The rear superior sextants commonly showed more 4 to 5 mm pockets, the front sextants calculus and the rear inferior sextants showed calculus and bleeding. 94.0 percent of the patients required periodontal treatment (3.6 sextants per patient average). Conclusions Periodontal treatment needs were high in this study; nine out of ten patients in the Mixteca region of the State of Puebla required periodontal treatment. Efforts must thus be guided towards improving oral health in indigenous communities.

[Periodontal treatment needs in adults from Mixteca rural area in Puebla State, Mexico]. / Necesidades de tratamiento periodontal en adultos de la región rural Mixteca del Estado de Puebla, México.

OBJECTIVE: This study was aimed at determining periodontal treatment needs, as determined by the Community Periodontal Index of Treatment Need (CPITN), in a sample of adults from the Mixteca region of the State of Puebla, in Mexico. MATERIALS AND METHODS: This was a descriptive, cross-sectional, single-centre study. Previous informed consent was obtained; 60.0 % of the sample were women whose main activity was housework (46 %), 14.0 % were farmers. Average age was 37.6 ± 13.6. Gingival and Community Periodontal Index of Treatment Need proposed by the World Health Organisation and the International Dental Federation were used; they were implemented by the same, previously-standardised researcher. Means, standard deviations and confidence intervals were calculated for dimensional variables and percentages for categorical ones. RESULTS: The gingival index gave 50.0 % light gingivitis, 32.0% moderate and 14.0 % severe gingivitis. The rear superior sextants commonly showed more 4 to 5 mm pockets, the front sextants calculus and the rear inferior sextants showed calculus and bleeding. 94.0 % of the patients required periodontal treatment (3.6 sextants per patient average). CONCLUSIONS: Periodontal treatment needs were high in this study; nine out of ten patients in the Mixteca region of the State of Puebla required periodontal treatment. Efforts must thus be guided towards improving oral health in indigenous communities.