Subject(s)
Antibodies, Anti-Idiotypic/genetics , Arthritis, Rheumatoid/genetics , Epitopes/genetics , Genetic Predisposition to Disease , Peptides, Cyclic/genetics , Age Factors , Age of Onset , Antibodies, Anti-Idiotypic/immunology , Arthritis, Rheumatoid/immunology , Cross-Sectional Studies , Epitopes/immunology , Female , Genotype , HLA-DR Antigens/genetics , HLA-DRB1 Chains , Humans , Male , Middle Aged , Peptides, Cyclic/immunology , Time FactorsABSTRACT
Introducción. La interleucina-10 (IL-10) es una citocina antiinflamatoria que se ha implicado en la patogenia de los aneurismas de la aorta abdominal (AAA). Se han descrito variaciones en el promotor de su gen que pueden condicionar su producción, y alterar el equilibrio entre citocinas pro y antiinflamatorias. Objetivo. Estudiar el genotipo de los microsatélites y polimorfismos del promotor de la IL-10 para valorar las posibles predisposiciones genéticas en la población española portadora de un AAA. Sujetos y métodos. Se realizó el tipado de los microsatélites asociados (IL-10R e IL-10G) y de las tres posiciones polimórficas (-1082, -819, -592) del promotor de la IL-10 en un grupo de 72 pacientes con un AAA, y se comparó con una muestra control de 349 sujetos sanos. Resultados. Microsatélites asociados: no se encontraron diferencias significativas al analizar el patrón fenotípico de los microsatélites IL-10G e IL-10R entre los pacientes con un AAA y el grupo control. Polimorfismos puntuales: no se encontraron diferencias significativas al analizar los diferentes haplotipos del promotor del gen de la IL-10 entre los pacientes con un AAA y el grupo control. Conclusiones. A la vista de nuestros resultados, en la población española no existe una relación entre ninguno de los diferentes alelos de los microsatélites IL-10R e IL-10G asociados al promotor de la IL-10 y la presencia de un AAA en dicha población. Tampoco existe una relación entre los diferentes haplotipos del promotor de la IL-10 y esta enfermedad en nuestro medio
INTRODUCTION. The anti-inflammatory cytokine interleukin-10 (IL-10) has been implicated in the pathogenesis of the abdominal aortic aneurysm (AAA). Changes in the promoter region of the IL-10 gene have been found to condition its production, altering the balance between pro- and anti-inflammatory cytokines. AIM. Associated microsatellites and polymorphisms in the IL-10 promoter were typed in an attempt to identify a possible genetic predisposition for AAA in the Spanish population. SUBJECTS AND METHODS. Associated microsatellites (IL-10R and IL-10G) and the three polymorphic loci (-1082, -819, -592) of the IL-10 promoter were determined in 72 patients with AAA and compared with those established in 349 healthy control subjects. RESULTS. No significant differences were observed between the AAA and control group in the phenotypic patterns shown by the microsatellites IL-10G and IL-10R nor in the different haplotypes of the IL-10 gene promoter. CONCLUSIONS. Our findings suggest no relationship between the different alleles of the IL-10R and IL-10G microsatellites and AAA in the Spanish population. We were also unable to detect a clear association between the different IL-10 promoter haplotypes and this disease
Subject(s)
Male , Middle Aged , Humans , Interleukin-10/administration & dosage , Aortic Aneurysm, Abdominal/complications , Aortic Aneurysm, Abdominal/diagnosis , Aortic Aneurysm, Abdominal/genetics , Autoimmunity/genetics , Risk Factors , Interleukin-10/genetics , Aneurysm/diagnosis , Aneurysm/genetics , Hypertension/complications , Myocardial Ischemia/complications , Tobacco Use Disorder/genetics , Tobacco Use Disorder/pathologyABSTRACT
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that may be an important regulator in multiple sclerosis (MS) pathogenesis. IL-10 gene contains three single nucleotide polymorphisms (SNPs) and two polymorphic microsatellites in the 5'-flanking region. Our aim was to ascertain if any of these polymorphic markers is associated or linked to MS among Spanish patients. We have genotyped 300 patients and 357 ethnically matched controls for the microsatellites, and most of them also for the promoter SNPs. We included in this study the parents of 63 patients in order to perform a TDT analysis as well. IL-10G12 allele was significantly increased in MS patients (17% versus 11% in controls; p=0.004; p(c)<0.05). No other allele showed a significant difference between patients and controls, and the TDT analysis yielded negative results. The data indicate that IL-10 is not a major susceptibility locus in MS, but in our population it might, however, have a minor role.
